Posts of the Category: Photography

This category deals with everything related to taking pictures through the microscope

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Testing the iPad camera

The two images show the fresh water polyp Hydra sp taken with the iPad held in front of the eyepiece (40x and 400x total magnification). The images were uploaded directly and not edited (except resize).

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The camera has a reduction lens and can be mounted on the phototube.

Connecting a camera to a microscope

There are several solutions for connecting a camera to a microscope. In this post I want to show you some of the ways that I used in the past and the advantages and disadvantages of each approach.

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rose

How to make macro images

This time I’d like to talk about a topic which is only indirectly related to microscopy: macro imaging. Taking high-quality macro images can be quite a challenge and can involve quite a bit of trial and error until one has found the ideal conditions. The pictures of the rose have been taken with a Sigma [...]

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dandelion

Dandelion parachute ball up close

This is one of the first tries taking pictures with my new Sigma objective, and I have to admit that I’m very satisfied with the lens. The lens does not include image stabilization, a steady tripod is therefore a must. Contrast was slightly enhanced to make the background (my computer screen!) completely black. Mirror lock-up [...]

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human hair

Human hair under the microscope

Today I’d like to show you a nice microscopic picture, which I took several years ago of two human hair. The pictures on the right show you the original, unprocessed image at the top, and a second cleaned-up image with a nice background on the bottom. The top image shows darkened corers from the field [...]

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Field diaphragm is wide open. Reflections from the side of the tube are very strong.

Köhler illumination to reduce reflections

The Köhler (or Koehler or Kohler) field diaphragm is located above the light source. It is responsible for controlling the width of the light beam (but not its intensity). The light source of a microscope without Köhler illumination will illuminate the whole specimen, which may be the source of stray light and excessive heating of [...]

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Digital methods for improving microscopic photographs

Digital photography gives the users many new possibilities in improving photographs taken through the microscope. This post gives an overview of the different image processing functions that can be applied to microscopic images. This post places a focus on what is possible, but does not explain the “how” part. This is something that I plan [...]

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Comparison of resolution. There is practically no visible difference between 3MP and 12 MP. The limiting factor is therefore the microscope or specimen, and not the camera resolution.

Required camera resolution for photography through the microscope

My digital camera (a Canon EOS 450d) is capable of taking pictures at 3MP, 6 MP and 12 MP (MP=megapixels). Which setting should one choose to obtain the optimum results? If the camera resolution is too low, then this will result in the loss of image detail (but a small file). A resolution setting which [...]

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