The resolution that a microscope is capable of achieving is probably the single most important factor that determines the quality of a microscopic image. Without a sufficiently high resolution, magnification is not possible without loss of quality. There are a variety of different factors that determine the achievable resolution. Some of these factors can not be actively influenced by the microscopist, others can. Some of the factors play a larger role, others a smaller one. In the following post, I want to summarize some of these factors.
The thickness of the cover glass can have a significant impact on the resolution.
The mounting medium can have a significant effect both on the image quality and on the specimen itself.
Köhler illumination reduces stray light and therefore increases contrast when taking pictures.
In this post I’d like to show you a method of making patch stop and color filters using a printer.
Phase Contrast microscopy makes specimens of low contrast appear with greater contrast.
There are different ways of digitizing analog slides.
Here I would like to show you two images of potato starch grains taken with different optical contrasting methods.
Drawing is still a useful method for documenting microscopic specimens, despite advances in (digital) imaging technologies. There are certain advantages in drawings that photographs do not possess.
Image editing software can be useful to enhance the contrast of photomicrographs. This article presents a short overview of possible adjustments.