Category: Techniques

 

How to obtain the best resolution with your microscope

The resolution that a microscope is capable of achieving is probably the single most important factor that determines the quality of a microscopic image. Without a sufficiently high resolution, magnification is not possible without loss of quality. There are a variety of different factors that determine the achievable resolution. Some of these factors can not be actively influenced by the microscopist, others can. Some of the factors play a larger role, others a smaller one. In the following post, I want to summarize some of these factors.



Cover glass thickness and resolution

The thickness of the cover glass can have a significant impact on the resolution.



Ranunculus pollen mounted in air, with cover glass.

The effect of the mounting medium on specimen and image quality

The mounting medium can have a significant effect both on the image quality and on the specimen itself.



Köhler illumination to reduce reflections

Köhler illumination reduces stray light and therefore increases contrast when taking pictures.



How to make microscope filters

In this post I’d like to show you a method of making patch stop and color filters using a printer.



Bacteria in phase contrast

Phase Contrast microscopy makes specimens of low contrast appear with greater contrast.



Digitizing photographic slides with a digital camera

There are different ways of digitizing analog slides.



Potato starch grains in bright field.

Potato Stach Grains

Here I would like to show you two images of potato starch grains taken with different optical contrasting methods.



Drawing Microscopic Images

Drawing is still a useful method for documenting microscopic specimens, despite advances in (digital) imaging technologies. There are certain advantages in drawings that photographs do not possess.



Enhancing Photomicrographs

Image editing software can be useful to enhance the contrast of photomicrographs. This article presents a short overview of possible adjustments.




 
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