Category: Theory

 

Observing Brownian motion

Brownian motion is the random movement of particles. This motion can be observed under the microscope, when looking at very small particles. In this case we will have a look at the fat droplets in milk.



What are the differences between aqueous and non-aqueous monting media?

Mounting media for microscopy can roughly be divided into those that are water soluble (aqueous) and those that are not (non-aqueous).



Ranunculus pollen mounted in air, with cover glass.

The importance of mounting medium refractive index

The refractive indes (RI) of the mounting medium can have a significant impact on the image quality.



Glass bulb

Why should you never touch the bulb of a microscope lamp?

Microscope bulbs should never be touched with bare hands, as the grease deposited on the lamp will cause its breakage.



Blue daylight filter

What are the advantages of using a blue filter for microscopy?

A blue daylight filter is able to remove red parts of the color spectrum when using tungsten or halogen lamps, giving the micrograph a cooler color.



Concave slide

What are the pros and cons of concave microscope slides?

Concave microscope slides have either one or two depressions to hold larger specimens.



What is Köhler illumination?

Köhler (Koehler) illumination significantly increases the image quality when doing photographic work.



What objective types are there for compound microscopes?

There are several ways in which the different microscopes objectives can be grouped.



How much magnification do I need?

Your compound microscope should be able to magnify from 40x to 400x (having a 4x, 10x, 40x objective).



What are the differences between achromatic and apochromatic objectives?

The different colors of light do not behave equally when passing through the lens system of the objective.




 
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