Posts of the Category: Theory
What are the pros and cons of concave microscope slides?
Concave microscope slides have either one or two depressions to hold larger specimens.
What is Köhler illumination?
Köhler (Koehler) illumination significantly increases the image quality when doing photographic work. It reduces stray light by only illuminating the part of the specimen which is actually being observed. The higher the magnification of the objective, the smaller the area which can be seen. Köhler illumination limits the light only to the part of the […]
What objective types are there for compound microscopes?
There are several ways in which the different microscopes objectives can be grouped. It is well possible that a particular objective belongs into several groups at the same time. Achromatic objectives: These are the standard objectives that can be found on most microscopes. There might be a slight chromatic aberation (color errors) Apochrmatic objectives: These […]
How much magnification do I need?
Your compound microscope should be able to magnify from 40x to 400x (having a 4x, 10x, 40x objective). A 1000x oil immersion objective is nice to have, but the range of applications are limited. The total magnification of the microscope is calculated by multiplying the magnification of the objectives, with the magnification of the eyepiece […]
What are the differences between achromatic and apochromatic objectives?
The different colors of light do not behave equally when passing through the lens system of the objective. The red components of the light are focused differently than the blue components. As a consequence, objects appear to have colored fringes around them and the image appears not as sharp, an effect which is referred to […]