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Some algae
June 5, 2014
10:48
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I.  I'm not sure about the genus, but my guess is that it might be Tribonema.  The right half of the image was cut then pasted below the left half to provide a larger view. 40x objective.

Bright field:

http://i1070.photobucket.com/albums/u494/micritter/images%20through%20the%20microscope/P5257841csM_40x.jpg~original

Cross-polarized light + retarder:

http://i1070.photobucket.com/albums/u494/micritter/images%20through%20the%20microscope/P5257850csM_40x.jpg~original

 

II. Flat colonial algae (a few micrometers thick) apparently covered by mucilage. Could this be a Pediastrum species?  40x objective.

Oblique lighting:

http://i1070.photobucket.com/albums/u494/micritter/images%20through%20the%20microscope/P5288174cM_40x.jpg~original

DIC:

http://i1070.photobucket.com/albums/u494/micritter/images%20through%20the%20microscope/P5288176cM_40x.jpg~original

June 5, 2014
12:12
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Hi Gekko ,sorry  no help with the identification, my meatball method is to search for images online and hope to stubble across what I am looking for. I  find that the old  black and white drawings are often the best for ID purposes   I will keep it in mind though. I have found a similar algae in the garden pond

Nice set of images I particularly  like the oblique light image of the colonial algae the membrane shows up nicely.

 

Thanks

Molehill

June 5, 2014
12:22
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Thank you, molehill, for your kind comments.  I have looked in four reference books that I have, and searched in Google Images for "green algae", "colonial algae" but, given that what I'm looking for is flat rather than globular, Pediastrum was the only thing that I found that came somewhat close, even though it doesn't look like the "proper" Pediastrum as shown in Seb Vasselle's recent image. Thanks again!

June 6, 2014
16:03
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Hi gekko

I'm guessing again but could it be a Eudorina sp. or a Stephanosphaera sp  - look them up on the web and see if you think they are anything like what you have.

Tony

East Riding of Yorkshire(UK)

nice imagesSmile

June 6, 2014
19:52
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Hi Tony,

Thank you for your suggestions.  Unfortunately, both Eudorina and Stephanosphaera, as far as I could determine, are spherical or globular colonies. The images I posted are for a flat, one-cell deep (I believe) colony– perhaps 5 – 10 µm deep.  I thought that perhaps it was a species of Pediastrum because that was the only genus of colonial algae of this size that I could find that was flat (non-globular) and sheathed in mucilage, but that doesn't mean I'm right, of course (I rarely amSmile).

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