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How do I make a wet mount?

Found in: Howto, Labwork

The wet mount is the most common way of preparing objects for microscopic observations.

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In your first wet mount we will be looking at a small amount of milk. Take a microscope slide and place a drop of water on the center of the slide. Then transfer a small droplet of milk into the water drop and mix carefully. You can use toothpicks or tweezers to do this. By mixing the milk with the water we make sure that the concentration of the fat droplets in the milk is not too high, otherwise it will be more difficult to see anything. Take a cover glass at its edge sand carefully lower it on the milk water mixture.

Carefully knock against the slide and look at the cover glass. does the cover glass move because it swims on top of the liquid? Does any liquid emerge beneath the cover glass? If this is the case, then you have used too much water or milk and should use some tissue paper to remove any excess water. too much visible water also runs the risk of making the objective wet. Then place the slide on the stage and start observing using the low power objective lens. only when the image is in focus should you use a larger magnification.

When making a wet mount, generally make sure that your sample is of the right size. If the specimen is too large, then prepare it first by cutting or tearing. Place a drop of water on the center of a slide Place the specimen into the water drop and make sure that it is completely covered by water. If necessary add an extra drop of water at the top. Carefully lower the cover glass at an angle on the water, this reduces air bubble formation. Remove excess water with tissue paper, or add more water with a dropper pipette at the side. Evaporating water can be replaced by adding more with a dropper pipette. The cover glass should not float, remove excess water with tissue paper.

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