MicrobeHunter.com Microscopy Forum

Beautiful!

Thoughtful and profound microscopy observations with your B/W image captures spark my my reaction, thank you hkv. It has always given me pause at how with 'blasts of transmitted illumination'...a sample wet mount slide prep is oh so unnaturally visualized. I toyed with 'night vision' device to see ...

Quite interesting: Botryococcus has a lot of potential as a source for renewable energy as it produces a lot of hydrocarbons: https://de.wikipedia.org/wiki/Botryococcus_braunii You have made this visible in a beautiful way, Håkan! Bob Yes, the algae have been used for biofuel production. I wrote th...

hb2sbdb wrote: ↑

Thu Feb 06, 2020 12:05 am

amazing! very arfull images. I could make poster out from any of them and hang in my living room (well, maybe except the nematode one

Ha ha, yes teh nematode may scare a few people.

MicroBob wrote: ↑

Tue Feb 04, 2020 5:25 pm

Isn't it the Botryococus that has oil drops at it's edges?

Yes, yes, I wrote the wrong name. Sorry!

Thanks all! Great to get feedback and I agree about the geometric shapes. MicroBob, I also like those two, but also Gloeotricha with the oil-drops around the edge.

Yes, unfortunately, DIC is not cheap. For some reason the prices refuse to drop on ebay. Used Olympus (BX) equipment are almost more expensive nowadays than they were 3-4 years ago.

/Håkan

I currently have phase, fluorescent, and dark-field capability but was wondering if anyone familiar with the BX series can tell me what I will need to take advantage of DIC on my particular setup. You need DIC prisms in the condenser. The condensers that take prisms are U-UCD8 and U-UCDB. DIC can b...

Hi folks, I have been experimenting with black and white micrographs for a change. I think it gives a different feeling and interesting contrast. Been trying BF, DF, DIC, POL and FLUO to see the difference. The First image is a colonial cyanobacteria (Gloeotrichia) in brightfield. The second image i...

viktor j nilsson wrote: ↑

Thu Sep 05, 2019 8:14 pm

Hej Håkan,
I've admired your work for quite some time. Good to see you post on here as well! Amazing and inspiring photos as always.

Viktor, in Lund

Thanks Viktor! Good to see a Swedish microscope fellow on here. Not many in Sweden that I am aware of with this hobby.

Wow these are really good!! Especially the last one. I'd be very interested to hear about your setup with the hope of improving my own technique. Cheers! Wes Hi, the setup was a BX51 with a UplanSAPO 60XW (Water immersion) for the first and last image. The rotifer was shot using the UplanSAPO20X. D...

Wonderful images hkv. Thanks for sharing. I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share?? Tom W. Thank you! Well, the body was still, but the head moved. Therefore the head is barely v...

Hi there, been lurking around here but not posted for some time. I thought I would share a few of my recent images with you. Not much time to photograph anymore, but was out sampling a local lake recently and managed to get a few samples. First time I manage to get Botryococcus! I remember 75RR had ...

If you alter the link a bit and use the server name "dl.dropboxusercontent" you will be able to display the image without users having to click on the link. It will be embedded in the post. Just a tip. Here is your link with the server name altered. Also remember to enclose the link with brackets ht...

Beautiful!

Wow! What a lab! Would be nice to visit someday!

Thanks! I appreciate your feedback!

Excellent images! Professional quality indeed! I like them very much!

Cool! Very interesting to see!

Hi Håkan, really great images! Every time I see how beautiful and detailed nature is it becomes more and more difficult to understand how badly we treat it. It's nice of you to decribe the amount of work and capability that was neede to make these images. So we are not disappointed when we can't ge...

Thanks Bob, What you say has just made me think. So does a larger drop of water create a larger space between the slide and the coverslip? Within reason. :) Yes, for sure. Large drop, large gap. The surface tension of the water lifts the glass sort of. I use Microbobs method as well. Add water with...

Your DIC images are great again! Where in Sweden do you live? I was twice paddling in vintage folding kayaks in the area south on Västervik with my family. It was very impressiv to move between these many small uninhabited islands and then stay the night in tiny tents on one of them. I live in Stoc...

Fascinating! Is the red photo - fluorescence ? the same diatoms as in the first DIC photo? Yes, fluorescent light. The red is the chlorophyll auto-fluorescing. Not very exciting as an image as such, but it clearly revealed 4 strings of chloroplasts in each diatom in the colony. If you look at the D...

Looks like Paramecium for sure! Great shots! The first one seems a bit stress as it is about to burst. Maybe because of pressure from the cover glass.

I was out with the boat collecting samples from a local lake and was surprised to get a huge amount of diatoms. Many which I have never encountered before. I dragged it after the boat for about 5 minutes just below the surface. My daughter was more interested to use the collecting device as a hat. I...

Grahame wrote:Neat images.
Have you tried a cross-eye stereo of any of them ?

No, I have not tried that. I have to struggle to see cross-eye stereo at all and mostly fail in doing so... Therefore, I never enable that feature when stacking. Would be neat to see these in 3D for sure.

Thank you all for your comments! The darkfield image is also one of my favourite.

Long time since I posted in this forum so I thought I would share a few images. I was out boating in august and noticed some small "algae" floating on the surface of the water. It was like small hairy balls and I took a sample in a beer can to check them out. To my surprise, they were not algae at a...

The flagella are also clearly visible using DIC.


Euglena by Håkan Kvarnström, on Flickr

Looks like Euglena. Nice shot!