MicrobeHunter.com Microscopy Forum

Monochrome emitters are plenty strong for brightfield fluorescence, even too strong in the blue range. Even with green (lime) which is the least efficient and thus weakest for a small emitter there was no issue on the scope I set up.

LED retrofits are not that difficult or expensive and don't tend to explode ever.

They can still be edited or deleted before the recipient logs in so it isnt a pointless feature either.

Oh wow, it's an actual trinocular head, not the photo addon. That's a valuable piece of equipment.

I think all the -x Olympus infinity heads are compatible, though some are better than others.

In this case there is a legitimate reason for the mount. The SZ7 has like double the reolution of other SZ family members and a fairly different optical system--its max NA of 0.2 handily beats Leica's current offerings in the series (and was equal to the recently discontinued S Apo). A normal SZ len...

They're not totally impossible to get. What magnification are you after? The sz7 is pretty sifferent from the others optically.

zondar wrote: ↑

Sat Mar 30, 2024 5:00 pm

I call on Abbe visit us with his ghostly apparition to set everything right!

That would make him an Abberration.

Oh, the normal plan 10x is a really good objective for its price, excellent corner to corner performance even direct on full frame and it also works surprisingly well with DIC, though I'd need to retest the best slider/condenser prism combo.

Sure Squintsalot wrote: ↑

Fri Mar 29, 2024 9:05 pm

You're right. That is an unusual scope. In fact, I wasn't even aware that Zeiss made wall-mounted microscopes.

Jokes aside they totally do for surgical applications.

Macro Cosmos has tried them and they're definitely better than the previoud sapo line. They're not brand new and they go back before the Evident selloff.

zondar wrote: ↑

Fri Mar 29, 2024 3:13 pm

Note: I'm NOT an expert in the physics of optical systems. Someone with real knowledge will come along and correct me soon enough if need be.

No offense but it sounds like Abbe did back in the late 1800s.

I've never had problems with canon efcs, it is vibration free during shooting.

They are largely Chinese but they also have a relationship with Meiji and sell some of their Japanese made microscopes under their own brand.

I'd watch for a favorable auction, not a buy it now. I got mine for 450, this one is starting at 600 but it's also VC (a little better than apo) https://www.ebay.com/itm/315246893929

Just saying this may be worth a bid https://www.ebay.com/itm/276399144621

The trinocular addon is quite valuable in its own right. You did well.

Using my planapo 60x/1.40 on my e800 was a bit disappointing since it has a dry condenser which throttles resolution. The oil condenser is very rare, I have never seen one for sale. I get better results using it with epi illumination/epi DIC on my other scope since it acts as its own high na oil con...

Yes but the f.N. only matches the real or viewed ield with a 10X eyepiece.because they are the default and the system's logic is based entirely on 10X eyepieces. It seems to me that the confusion is arising from thinking of it this way, even if there's nothing technically wrong with it. A 1x object...

Field number is based on a hypothetical 1x objective, not a 10x eyepiece. It's just a question what the diameter of the imaged field would be with a 1x objective, regardless of how large it looks to the eye.

First things first, get a cheap 20x/0.75 apo. Make sure it is the labeled one. It's an incredible objective and absurdly cheap because they were widely used in gene sequencers. The 20x fluor slider works pretty well with it. Edit: currently the price is a bit higher, the last glut was a while back. ...

I think the 1x also required a special upper lens like some Nikon cfi60s did a generation or two ago (1x macro).

Don't get an M3b imo unless it is quite cheap. Being a step mag isn't bad but only having 3 settings is a bit of a bummer vs 5 on the m3c or continuous on the m3z, the C is a basically strictly better with no optical downside, the m3z is somewhat nicer to use even if the zoom probably has some optic...

Congratulations! Looks like you're getting good shots.

That's a very good deal in my opinion. Good 580s are rare.

Btw I wanted to say thanks again for sharing your unique insights on all this.

B&L made at least one DIC system, epi DIC for the balplan. A rare but impressive piece of equipment. The AO epi is also pretty exceptional.

I understand why this was necessary in the 60s and earlier, but I do find it odd that Nikon was able to manage so well without additional corrections in the 80s, followed by Olympus and Mitutoyo, while Leica and Zeiss cling to it still. I kind of assume Leica and Zeiss should have the best engineers...

65mm thread objectives have an added 1.25x magnification on m50/m60 systems like the M8, so it would act as a ~0.78x. Leica plan apo stereo objectives are quite nice.

That .32X fractional lens is to compensate for the tube length and reduce unwanted alterations in W.D. It isn't needed if the tube length is kept to something close to the reference length. My understanding is thst, as designed, a viewing or afocal photo eyepiece projects an image at infinity focus...