Search found 219 matches

by Seb28
Tue Jun 23, 2020 11:35 pm
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3833

Re: My First Attempts at Digital Photomicrography

Congratulations on your first attempts. One thing is certain that your photos are too dark. I suggest you change the light in the microscope for a stronger one. Try to play with different white balance settings. I also suggest using the smallest ISO 100-400. I also allowed myself to copy your photo ...
by Seb28
Tue May 12, 2020 1:40 am
Forum: Pictures and Videos
Topic: Arachnoidiscus ehrenbergi
Replies: 16
Views: 2071

Re: Arachnoidiscus

Great images.
by Seb28
Sun May 10, 2020 3:23 pm
Forum: Beginner's corner
Topic: Imaging help using a Canon dslr
Replies: 20
Views: 1902

Re: Imaging help using a Canon dslr

23,2 mm is the standart size for eyepieces the adapter will fit.Which adapter you ordered?
by Seb28
Sat Apr 11, 2020 10:54 am
Forum: Pictures and Videos
Topic: Fluorescent Microorganisms
Replies: 3
Views: 500

Re: Fluorescent Microorganisms

Beautiful.
by Seb28
Tue Apr 07, 2020 2:23 pm
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 987

Re: Pine needle-fluorescence,

Thank you. great images! Are these permanent mounted or fresh? They were fixed in FAA,PEG1500 embbeded and hand cut. Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually r...
by Seb28
Tue Apr 07, 2020 9:26 am
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 987

Pine needle-fluorescence,

Two cross sections slices of pine needle staining in calcofluor white and acridine orange.
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b6.jpg
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b5 copy.jpg
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by Seb28
Sun Apr 05, 2020 9:46 pm
Forum: Pictures and Videos
Topic: Mucor sp.-spores.
Replies: 1
Views: 297

Re: Mucor sp.-spores.

by Seb28
Sun Apr 05, 2020 8:29 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 819

Re: Pine needle-safranin,fast green staining.

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?
I'm using PEG1500.
by Seb28
Sun Apr 05, 2020 6:19 pm
Forum: Pictures and Videos
Topic: Epistylis
Replies: 5
Views: 532

Re: Epistylis

Very nice.
by Seb28
Sun Apr 05, 2020 6:02 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 819

Re: Pine needle-safranin,fast green staining.

Hi Bob
I haven't tried to embbeding the sample into PEG yet and cut on a microtome, I am going to try it soon but I know that this technique is practiced but in harder PEG for example PEG4000.
Read this article.
https://scholarworks.uni.edu/cgi/viewco ... ntext=pias
by Seb28
Sun Apr 05, 2020 3:51 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 819

Re: Pine needle-safranin,fast green staining.

Thank you all for the comments. The embedding procedure in Peg1500 is as follows: After fixation in FAA, I transfer the sample to 20% PEG solution for several hours, preferably 24 hours, then transfer it to 90% PEG solution for several hours, and then transfer the sample from 90% PEG solution straig...
by Seb28
Sat Apr 04, 2020 9:51 pm
Forum: Microscopes and optics
Topic: Polyvar Dic system questions.
Replies: 8
Views: 2033

Re: Polyvar Dic system questions.

Thank you for your advice.
The Polyvar model what I talk abaut is this one.
s-l1600 (7) a copy.jpg
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s-l1600 (1).jpg
s-l1600 (1).jpg (124.14 KiB) Viewed 1763 times
Now I now what the parts are.
polyvar parts copy.jpg
polyvar parts copy.jpg (157.78 KiB) Viewed 1763 times
by Seb28
Sat Apr 04, 2020 9:34 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 819

Pine needle-safranin,fast green staining.

After many attempts I managed to stain the pine needle with safranin and fast green. This is not what I expected but so far this is the best result. I have to keep trying to get better results. The pine needle was fixed in FAA and then embedded in PEG1500 for better cutting.I cut the needle under a ...
by Seb28
Sat Apr 04, 2020 3:43 pm
Forum: Pictures and Videos
Topic: Haematococcus, Ciliate
Replies: 6
Views: 651

Re: Haematococcus, Ciliate

Beautiful.The video quality is amazing.Thanks for sharing.
by Seb28
Fri Apr 03, 2020 1:55 am
Forum: Specimens, samples and slides
Topic: Apple Twig Hand Section, Stain & Mount
Replies: 9
Views: 1138

Re: Apple Twig Hand Section, Stain & Mount

Great work.Very informative,thanks.
by Seb28
Fri Apr 03, 2020 1:47 am
Forum: Pictures and Videos
Topic: Mucor sp.-spores.
Replies: 1
Views: 297

Mucor sp.-spores.

Spores of mold Mucor sp. mag.1000x,DIC
by Seb28
Tue Mar 31, 2020 3:28 am
Forum: Pictures and Videos
Topic: Peritrichida
Replies: 9
Views: 980

Re: Peritrichida

Very nice images.Did you use the flash?
by Seb28
Mon Mar 23, 2020 12:21 am
Forum: Pictures and Videos
Topic: Trachyneis aspera (another)
Replies: 17
Views: 2588

Re: Trachyneis aspera (another)

Great image.
by Seb28
Mon Mar 23, 2020 12:19 am
Forum: Pictures and Videos
Topic: Water mite stereo
Replies: 13
Views: 2069

Re: Water mite stereo

Beautiful.
by Seb28
Sat Mar 21, 2020 4:51 pm
Forum: Microscopes and optics
Topic: Polyvar Dic system questions.
Replies: 8
Views: 2033

Re: Polyvar Dic system questions.

Thank you Francisco.
The condenser is Dic?
Do you you recognize what those two parts are on the left side in first photo?
by Seb28
Sat Mar 21, 2020 1:05 pm
Forum: Microscopes and optics
Topic: Polyvar Dic system questions.
Replies: 8
Views: 2033

Polyvar Dic system questions.

Does someone know is this a complete dic system for Polyvar microscope. ?
by Seb28
Sat Mar 07, 2020 6:11 pm
Forum: Pictures and Videos
Topic: fungus
Replies: 5
Views: 4218

Re: fungus

Beautiful.
What means "2.5pe"?Photo eyepiece?
I would also like to know what is your microscope setup.
by Seb28
Fri Mar 06, 2020 10:46 pm
Forum: Beginner's corner
Topic: Zeiss DIC- could this work?
Replies: 7
Views: 4737

Re: Zeiss DIC- could this work?

Hi William. A few years ago, when I didn't have a complete DIC system,I tried different combinations to achieve DIC. I get a good result with INKO prism epiplan 16/0.35.The prism was placed under the condenser and and worked with the PZO DIC head. Here is the link: https://www.photomacrography.net/f...
by Seb28
Tue Feb 04, 2020 12:43 pm
Forum: Pictures and Videos
Topic: tiny worm in soil
Replies: 4
Views: 1391

Re: tiny worm in soil

Look's like "Chaetogaster sp."
by Seb28
Wed Jul 31, 2019 1:36 pm
Forum: Pictures and Videos
Topic: Seagrass under microscope
Replies: 3
Views: 1713

Re: Seagrass under microscope

It looks like "Merismopedia sp." on the first picture.
on second picture probably Coleochaete sp.
by Seb28
Fri Apr 12, 2019 11:53 am
Forum: Specimens, samples and slides
Topic: Acridine Orange Protocol
Replies: 2
Views: 2811

Re: Acridine Orange Protocol

http://www.tandfonline.com/doi/abs/10.3109/10520296709115015?journalCode=ibih19 Stem, leaf, and bud tissue of sweet potato, tomato, and pepper were embedded in paraffin, sectioned, mounted, and stained with 0.01, 0.1 and 1% aqueous and 0.1% alcoholic solutions of acridine orange. Temporary and durab...
by Seb28
Sat Dec 17, 2016 4:13 pm
Forum: Pictures and Videos
Topic: Still Another Poinsettia
Replies: 9
Views: 1603

Re: Still Another Poinsettia

You can get much better effect staining by clearing the sample before staining in lactic acid or clove oil to get them more transparent.