MicrobeHunter.com Microscopy Forum

There are already many videos on youtube about DIY polarizers. I will show here a few specific tricks (smartphone polarizers, glass support, hair dryer, freezing wand, psychedelic field) and a lot of crystal growth.
Crystals start at 5:31

If you get lost in the gazillion illumination techniques and why they do what they do, I suggest this good course on abbe optics:
https://www.chalmers.se/sv/centrum/fysi ... bar/o1.pdf
knowledge about ray optics and interference required.

Anybody know how to upload a direct video that isn't vimeo or youtube? I have a video I want to upload but I can't seem to make a link to it. :| I tried HTML also... what error do you get when you make the link? Have you folowed the procedure below ? http://www.microbehunter.com/microscopy-forum/vi...

quite unrelated but I just got beautifull results playing with a dichroic cube

Thanks all for the clarifications, though I never paid much attention to the koelher focus. If the koelher was focused on the slide we'd see an image of the light source imprinted on the specimen right ? So it has to be out of focus anyways right ? Condenser element diameter also plays a role in con...

why would a condenser need to be achromatic/apochromatic ? As I understand this is not necessary/helpfull. If some colors have a bit more or less NA than others it's ok as long as they are all above the NA of the objective This is the way I understood it also... BillT In general, an incompletely co...

Like the 'ants eating sugar' Have you considered slowing the video down? thanks, please try the "speed" setting under the gear icon on bottom right I'd love to see ants eating a spider. I wouldnt know how to do that with the depth of field limitation on the x10 objective. Plus trouble sticking the ...

superbe, ca irait bien sur un t shirt

I think it's full after the last catch, and the neck retract for digestion

why would a condenser need to be achromatic/apochromatic ? As I understand this is not necessary/helpfull. If some colors have a bit more or less NA than others it's ok as long as they are all above the NA of the objective

What is your specimen? ant head, it is reflective, because the light moves with the Z axis , the overexposed speckle on the ant facing the light also spreads... IMHO, this object is not transparent, so it is better photographed under top illumination, light coming from above. You might achieve it w...

Hobbyst46 wrote:What is your specimen?

ant head, it is reflective, because the light moves with the Z axis , the overexposed speckle on the ant facing the light also spreads...

is there nothing that can be done by changing lenses diameters and distances while keeping the aperture diameter and magnification? I tried light sheet, on 4 and 10x it isnt super usefull because the depth of field is enough for stacking software. On x40 (stopped down) I didnt get enough light throu...

Hello,
If you were given complete control over microscope (using dslr camera) and objective design except for the absolute aperture size of the objective, what would you change to increase the depth of field at the magnification given by a 40x objective ?
Thanks

@hobbyist your results pictured above are consistent with mine. You had to push the iso (noise...) to see something, that's because most of the light is wasted. I suspect positive results were obtained using a much stronger illumination than mine in the connecting slide fixture, but i'm not even sur...

In order to get darkfield on x40 and x 100, I tried blasting light (just 3w led) on the edge of a slide a couple months ago, without much success. Even though I got great improvements by oiling the edge... In theory the change from air surface to water should help the light get out of the glass sli...

here are some last resort tips: -lower the iso if you're on a digital camera -make sure the iris, the stop, output of the condenser, objective are properly aligned (there are some tutorials and tips above) -make sure you dont have ambiant light parasiting ! -a good way to debug darkfield is to inser...

In order to get darkfield on x40 and x 100, I tried blasting light (just 3w led) on the edge of a slide a couple months ago, without much success. Even though I got great improvements by oiling the edge. My understanding is that you get internal reflection (like an optics fiber) in the glass slide i...

I havent succeeded stacking on regular 40x, I believe that's because the depth of focus is too thin for the software to process. I suggest you reduce the aperture of your objective to increase the depth of field

Hello Aquired a scope few months ago and started hacking and experimenting 1) basic setup and live snot (view in hd from 2:20), it's fun to see your own ciliate cells dancing ! JNMaHkiCeU0 2)then started playing with stacking (zerene staker software), but stacking requires a minimal depth of field a...