Search found 96 matches

by JGardner
Tue May 19, 2020 5:23 pm
Forum: Microscopes and optics
Topic: Best way to assess condition of microscope objective optics?
Replies: 8
Views: 180

Re: Best way to assess condition of microscope objective optics?

If one doesn't have a stereo microscope or a phase telescope, are there any techniques that can be used to evaluate objectives?
by JGardner
Wed May 06, 2020 9:40 pm
Forum: Microscopes and optics
Topic: Microscopes in the Movies
Replies: 6
Views: 496

Microscopes in the Movies

I just watched a miniseries called The Hot Zone about an Ebola outbreak in Reston Virginia. Among others, this movie featured the use of Zeiss Universal microscopes, electron microscopes, and ultramicrotomes. I've watched a few other pandemic-themed movies recently and many of them have glaring erro...
by JGardner
Tue May 05, 2020 10:20 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

The MSDS of IPA states that it should be kept in a tightly closed container. And that inhalation of the vapors may cause health problems. And for health and safety reasons should be stored in a ventilated area. IMO the simple plastic bottle from the store is OK as long as the plastic lid is tightly...
by JGardner
Sat May 02, 2020 8:29 pm
Forum: Microscopy accessories
Topic: What is it? Marker?? Oiler??
Replies: 8
Views: 1148

Re: What is it? Marker?? Oiler??

Perhaps it serves the same function as this:

https://flmicroscopes.com/collections/n ... ect-marker
by JGardner
Fri May 01, 2020 9:02 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

Hobbyst46 wrote:
Fri May 01, 2020 6:57 am
BTW, I apply the same rules to ethyl alcohol 95%.
I wonder how much ethyl alcohol vapor is in the air in a typical bar? It's probably significant.
by JGardner
Thu Apr 30, 2020 11:00 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

MichaelG. wrote:
Thu Apr 30, 2020 6:41 pm
JGardner wrote:
Thu Apr 30, 2020 5:18 pm
When I'm done with a slide, everything goes into a bucket filled with IPA. After a week or two in the bucket I pour off the alcohol ...
Ah but do you drink it ? :lol:
No. IPA = isopropyl alcohol, not India Pale Ale. ;)
by JGardner
Thu Apr 30, 2020 10:59 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

When I'm done with a slide, everything goes into a bucket filled with IPA. After a week or two in the bucket I pour off the alcohol and toss the slides/coverslips in the trash. I would avoid having any container of IPA that is not hermetically closed. Alcohol vapors are not toxic but are harmful. S...
by JGardner
Thu Apr 30, 2020 5:18 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

When I'm done with a slide, everything goes into a bucket filled with IPA. After a week or two in the bucket I pour off the alcohol and toss the slides/coverslips in the trash.
by JGardner
Wed Apr 29, 2020 8:55 pm
Forum: Beginner's corner
Topic: Newbie Question
Replies: 2
Views: 570

Re: Newbie Question

Hi Rodger,

You achieve depth of focus using stacking, as you already know. It's not difficult to do, but it takes time and practice to get it right.

As for perfect backgrounds, I suspect that this is achieved with judicious use of the Photoshop cloning tools to clean up messy backgrounds.
by JGardner
Tue Apr 28, 2020 6:36 pm
Forum: Microscopes and optics
Topic: Leitz PL APO vs NPL Fluorotar
Replies: 8
Views: 821

Re: Leitz PL APO vs NPL Fluorotar

Hi, I can comment a little, having had a set of 170mm TL planapo objectives on a Leitz (170mm TL) Orthoplan 'scope. The quality of those objectives is, in my experience, superb How do these objectives compare with modern infinity optics, like those for the Olympus BX scopes? I'm still pondering upg...
by JGardner
Mon Apr 27, 2020 8:07 pm
Forum: Beginner's corner
Topic: Reliable Dealers
Replies: 4
Views: 644

Reliable Dealers

Anyone have a list of microscope dealers known to be reliable? I've heard a few names, such as Brunel, Microscope Central, and Munday Scientific mentioned on these forums, but have no idea if any of these are reliable or if there are others that are. For example, when a dealer advertises a used micr...
by JGardner
Mon Apr 27, 2020 7:56 pm
Forum: Beginner's corner
Topic: Need advice buying a Reichert Microstar IV
Replies: 23
Views: 1704

Re: Need advice buying a Reichert Microstar IV

It came on Saturday afternoon. And now I'm reminding / convincing myself that I love to fix things....even though I thought I was buying something that was fully functional.... This one definitely is not. Welcome to the wonderful world of buying "fully functional" microscopes on eBay! I think nearl...
by JGardner
Fri Apr 24, 2020 10:21 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3246

Re: Specimen ethics

Total beginner question (I don’t even own a microscope...yet). When you prepare a slide with microbes, what do you do with them after you are done? I would feel bad after observing and enjoying them not to be able to place them back into the jar/pond/wherever I got them! Do you feel bad every time ...
by JGardner
Fri Apr 24, 2020 4:24 am
Forum: Beginner's corner
Topic: Also Needing Microscope Purchase Advice
Replies: 35
Views: 1679

Re: Also Needing Microscope Purchase Advice

I agree about the fine focus. The stage moves such a tiny amount when turning the fine focus that you may think it’s not doing anything. You’ll know for sure when you get the lighting sorted and can try focusing on a specimen.
by JGardner
Fri Apr 17, 2020 8:29 pm
Forum: Microscopes and optics
Topic: Kohler Issue
Replies: 1
Views: 188

Kohler Issue

I recently got a replacement stage for my Zeiss Universal, which has been out of service for some time. The new stage works fine, but I'm having issues setting up Kohler illumination that I don't remember having before. I checked the stage alignment, and it's as it should be, so no problems there. T...
by JGardner
Thu Apr 16, 2020 11:36 pm
Forum: Microscopes and optics
Topic: Retrodiode LED conversion
Replies: 15
Views: 680

Re: Retrodiode LED conversion

If you want something more robust and professional looking, there's always Nanodyne, but their products are in the $500-600 range.

https://www.nano-dyne.com/led-microscope-lights/
by JGardner
Tue Apr 14, 2020 7:54 pm
Forum: Microscopes and optics
Topic: Retrodiode LED conversion
Replies: 15
Views: 680

Re: Retrodiode LED conversion

I use the older version of their Orthoplan LED conversion. It works well, with one exception: it uses PWM to control LED brightness, so if the control is set to less than 100% I see bands when taking photographs through the microscope. One big advantage LED has over incandescent illuminators is a co...
by JGardner
Tue Apr 07, 2020 9:36 pm
Forum: Microscopes and optics
Topic: Orthoplan Serial Numbers
Replies: 1
Views: 124

Orthoplan Serial Numbers

Anyone know of a site or reference I can use to determine when an Orthoplan was built based on its serial number?
by JGardner
Sun Apr 05, 2020 12:09 am
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

I cleaned up the new stand and moved the parts over from my old Orthoplan stand and everything is working very well. It's nice to have a working field diaphragm and to have butter smooth focusing. Now I need to start hunting eBay for some objectives. It currently has a no-name 10x, a Leitz 32x and a...
by JGardner
Wed Apr 01, 2020 7:41 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

My new Orthoplan stand arrived today. It's much nicer than the stand of my original Orthoplan. This one's a "white" stand, which means it's one of the last ones made. The date stamp on the bottom of the stand indicates it was made in 1982. Functionally, this stand is much nicer than the old one. The...
by JGardner
Thu Mar 26, 2020 4:19 pm
Forum: Microscopes and optics
Topic: 160mm Objectives on Orthoplan
Replies: 13
Views: 963

Re: 160mm Objectives on Orthoplan

Those 2mm would not seem to be critical according to Leitzcycler Thanks for clarifying. I'll look for a 160mm objective on eBay to try before I look for a whole set. Anyone know how much degradation is visible when using 160mm objectives at magnifications less than 16x on an Orthoplan? I'm thinking...
by JGardner
Thu Mar 26, 2020 4:09 pm
Forum: Microscopes and optics
Topic: 160mm Objectives on Orthoplan
Replies: 13
Views: 963

Re: 160mm Objectives on Orthoplan

That's a nice chart, but it seems to cover every scenario except the one I'm interested in, e.g. 160mm objectives and 170mm eyepieces on a 170mm microscope You are describing #6 on the chart. When using a 160 objective on your Orthoplan raise the 170 eyepiece 8mm with a spacer. (That will bring the...
by JGardner
Thu Mar 26, 2020 3:54 pm
Forum: Microscopes and optics
Topic: 160mm Objectives on Orthoplan
Replies: 13
Views: 963

Re: 160mm Objectives on Orthoplan

75RR wrote:
Thu Mar 26, 2020 9:00 am
... (see example #6 on chart)
Dang! That's a nice chart, but it seems to cover every scenario except the one I'm interested in, e.g. 160mm objectives and 170mm eyepieces on a 170mm microscope.
by JGardner
Wed Mar 25, 2020 11:10 pm
Forum: Microscopes and optics
Topic: 160mm Objectives on Orthoplan
Replies: 13
Views: 963

Re: 160mm Objectives on Orthoplan

Thanks! That's exactly the first-hand experience I was hoping to hear.
by JGardner
Wed Mar 25, 2020 9:14 pm
Forum: Microscopes and optics
Topic: 160mm Objectives on Orthoplan
Replies: 13
Views: 963

160mm Objectives on Orthoplan

Sorry to open this can of worms again. I've read various articles on using 160mm objectives on Orthoplans, and I've read the Leitz memo addressing this topic. Basically, the memo says that 160mm objectives of 16x or higher magnification work fine on 170mm tube length microscopes such as the Orthopla...
by JGardner
Tue Mar 24, 2020 11:00 pm
Forum: Microscopes and optics
Topic: Zeiss Universal Focusing Block
Replies: 5
Views: 825

Zeiss Universal Focusing Block

Hi Zeiss experts! Are focusing blocks with all-metal focusing knobs compatible with Universals with Plastic focusing knobs? Can I do a one-for-one swap? My Universal (with plastic knobs) has very stiff focusing, and I've found a focusing block that's much smoother and need to know if they're interch...
by JGardner
Tue Mar 24, 2020 7:40 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

Several people who know I'm interested in microscopy have asked me recently if I can see Corona virus with my microscopes. I tell them nope, you need a transmission electron microscope for that. I wonder if any amateurs have a TEM?
by JGardner
Tue Mar 24, 2020 6:35 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

I see that the late-model Orthoplan you linked to is still awaiting ... second thoughts? It should show as sold--that's how it appears here when I click on the link. I've already gotten the confirmation from eBay. Who knows when it'll ship and when I get it, however, due to the Corona virus situati...
by JGardner
Tue Mar 24, 2020 5:36 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

Perhaps this is a question of life strategy. Which includes bad decision making. The problem of course has been identified as a problem with used microscopes in general, not the problem of which specific ones were purchased. The problem with used microscopes is that they're, well, used . Many of th...
by JGardner
Mon Mar 23, 2020 8:23 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15990

Re: Frustration!

So, I will have an Orthoplan with all important mechanical parts and systems set for life (or several). Plus spares. This system is one that I am building not just for myself but for my children and perhaps their children, too :-) I found what looks like a late-model Orthoplan stand in very good co...