Search found 760 matches

by viktor j nilsson
Tue Feb 06, 2024 4:36 pm
Forum: Microscopes and optics
Topic: Determining the back focal plane of an objective
Replies: 5
Views: 522

Re: Determining the back focal plane of an objective

The method described by Paolo Pozzi in the thread below is the best I've found: https://www.researchgate.net/post/Is_there_a_rule_of_thumb_to_determine_the_back_focal_plane_of_objective_lens I've used it to measure the location of the back focal plane of a bunch of Nikon MPlan objectives, as I wante...
by viktor j nilsson
Fri Feb 02, 2024 4:34 pm
Forum: Microscopes and optics
Topic: Curious Nikon PlanApo 40X objective
Replies: 2
Views: 392

Re: Curious Nikon PlanApo 40X objective

I think 40x oil objectives are a pleasure to use, not only as a companion to 100x objectives, but also as an optically superior and much less finicky alternative to 40x 0.95 dry objectives. That said, I've never felt the need to buy this older-generation CF planapo (that never got an updated CFN ver...
by viktor j nilsson
Sun Jan 28, 2024 10:16 pm
Forum: Microscopes and optics
Topic: Which 40x and 4x objectives for Nikon Labophot?
Replies: 16
Views: 1133

Re: Which 40x and 4x objectives for Nikon Labophot?

The CFN 4x in particular should give an really clean image when used properly. It does seem like something is off.
by viktor j nilsson
Fri Dec 29, 2023 1:57 pm
Forum: Microscopes and optics
Topic: PZO mst 127 focus repair
Replies: 4
Views: 3941

Re: PZO mst 127 focus repair

5x eyepieces are a bad idea for visual use. They cover the same real field of view as 10x eyepieces, but shrinks it down to a much smaller apparent field of view. Like looking down a roll of toilet paper. A pair of the good and cheap 10x/22 would be a better option.
by viktor j nilsson
Wed Dec 20, 2023 4:54 pm
Forum: Microscopes and optics
Topic: Thoughts on these microscopes for sale on Facebook appreciated
Replies: 7
Views: 7017

Re: Thoughts on these microscopes for sale on Facebook appreciated

Then why does one condenser position say 'DIC'?

I'm certainly not an expert on Leica scopes of this era.

Edit: the one mounted on the scope is of course a Hoffman condenser. I'm referring to the one mounted to the column on the table, which looks like associated with the Zeiss eyepieces.
by viktor j nilsson
Sun Dec 10, 2023 9:22 pm
Forum: Home-made microscope adaptations
Topic: Working in an "universal condenser" for Nikon Optiphot
Replies: 82
Views: 70178

Re: Working in an "universal condenser" for Nikon Optiphot

Sorry I haven't read everything in detail. But have you thought about the position of the interference fringe relative to the back focal plane of your objectives? I've measured the location of the BFPs of some of the objectives for which your prisms were made. The data is here: https://www.photomacr...
by viktor j nilsson
Tue Nov 21, 2023 5:33 pm
Forum: Illumination Techniques
Topic: Help: has anyone ever used Olympus DCW Darkfield condenser?
Replies: 31
Views: 92877

Re: Help: has anyone ever used Olympus DCW Darkfield condenser?

Your DF condenser should be a mirror/reflectingcondenser of some type. Perhaps Viktor knows which for sure. The degree of ca produced in high N.A. DF condensers is from very low to none. Paraboloids have a small amount, spherical too but cardioids, none. ... I did some checking and the BH2-DCW cond...
by viktor j nilsson
Mon Nov 20, 2023 7:42 pm
Forum: Illumination Techniques
Topic: DIC with Leitz Wetzlar Dialux
Replies: 18
Views: 5964

Re: DIC with Leitz Wetzlar Dialux

Frankiev wrote:
Mon Nov 20, 2023 5:57 pm
I'm going to hang fire on my DIC search for now because my immediate need is to find a x25 phase objective for my Leitz Wetzlar Dialux. Are there any for sale out there?
http://www.thilo-immel-optics.de/komplettmikroskope.htm

Thilo Immels has a complete Dialux ICT system for sale for €4950.
by viktor j nilsson
Thu Nov 09, 2023 7:09 am
Forum: Illumination Techniques
Topic: autoflorescence
Replies: 9
Views: 5715

Re: autoflorescence

This approach works well at lower magnifications:
https://www.photomacrography.net/forum/ ... hp?t=33123

Results here:
https://www.photomacrography.net/forum/ ... hp?t=33112

But be careful, UV damage is real.
by viktor j nilsson
Wed Nov 01, 2023 6:06 pm
Forum: Microscopes and optics
Topic: Nikon Fluor 40x frozen collar + front element
Replies: 18
Views: 8320

Re: Nikon Fluor 40x frozen collar + front element

IMG_20231101_190207.jpg
IMG_20231101_190207.jpg (105.77 KiB) Viewed 7985 times
Here's my 40x 0.85. It does look like your screw is not original.
by viktor j nilsson
Tue Oct 31, 2023 8:34 am
Forum: Microscopes and optics
Topic: Nikon Fluor 40x frozen collar + front element
Replies: 18
Views: 8320

Re: Nikon Fluor 40x frozen collar + front element

I got my kit from Newgate Simms several years ago, before Brexit. But I can't find it for sale at the moment. It has definately gone in and out of availability many times of the years. I don't think it is critical to get that exact kit. Many skilled microscopists have had good sucess using various b...
by viktor j nilsson
Tue Oct 31, 2023 7:22 am
Forum: Microscopes and optics
Topic: Nikon Fluor 40x frozen collar + front element
Replies: 18
Views: 8320

Re: Nikon Fluor 40x frozen collar + front element

karhukainen wrote:
Tue Oct 31, 2023 6:39 am
Thanks! Which lubricant/oil do you use?
The light oil from the Nye hobbyist kit.
by viktor j nilsson
Tue Oct 31, 2023 5:04 am
Forum: Microscopes and optics
Topic: Nikon Fluor 40x frozen collar + front element
Replies: 18
Views: 8320

Re: Nikon Fluor 40x frozen collar + front element

I've fixed the collar on a very similar Nikon CFN Planapo 40x 0.95. You just loosen the screws, which releases the rotating ring. Then you can clean and lubricate the mating surfaces. I don't think they are locked in place, but maybe add a tiny drop of acetone on each screw just in case. Although in...
by viktor j nilsson
Fri Oct 27, 2023 5:31 am
Forum: Camera systems and imaging
Topic: Zeiss AxioCam HRc - Worth the bother?
Replies: 30
Views: 25169

Re: Zeiss AxioCam HRc - Worth the bother?

There's a micro manager device adapter for it, so definitely worth a shot.

https://micro-manager.org/AxioCam
by viktor j nilsson
Fri Oct 20, 2023 1:44 pm
Forum: Microscopes and optics
Topic: leica dm 2000 with DIC objective compatiblity
Replies: 11
Views: 4512

Re: leica dm 2000 with DIC objective compatiblity

Seeing the venerable Leitz brand on a scope like that is kind of painful.
by viktor j nilsson
Wed Oct 18, 2023 8:19 pm
Forum: Microscopy accessories
Topic: Wild polarizer part ?
Replies: 4
Views: 2406

Re: Wild polarizer part ?

58mm matches the diameter of my Wild M5 objective.
by viktor j nilsson
Sat Oct 14, 2023 6:29 am
Forum: Illumination Techniques
Topic: Interchangeable DIC prisms (shear values for different manufacturers)
Replies: 11
Views: 5795

Re: Interchangeable DIC prisms (shear values for different manufacturers)

There's this: Screenshot_20231014-081850~2.jpg From: https://www.researchgate.net/publication/236224633_Quantitative_orientation-independent_differential_interference_contrast_microscope_with_fast_switching_shear_direction_and_bias_modulation Also this: Screenshot_20231014-082347~2.jpg From: https:/...
by viktor j nilsson
Sun Sep 24, 2023 6:00 am
Forum: Camera systems and imaging
Topic: Zeiss AxioCam HRc - Worth the bother?
Replies: 30
Views: 25169

Re: Zeiss AxioCam HRc - Worth the bother?

Totally understand that. I have a very fancy motorized Nikon Diaphot 300 with DIC and fluorescence (motorized stage, shutters and LEP mac2002 controllers from Ludl). The plan is that someday I'll get it to work with micro controller. But it's a bit daunting process to get everything setup, so it's b...
by viktor j nilsson
Sun Sep 24, 2023 5:00 am
Forum: Camera systems and imaging
Topic: Zeiss AxioCam HRc - Worth the bother?
Replies: 30
Views: 25169

Re: Zeiss AxioCam HRc - Worth the bother?

Thanks Michael, I had missed this development.. However, it continues. The links were dead, but this is what is now found on Open-imaging.com: Open Imaging is Closing Open Imaging is no longer conducting new business. For Micro-Manager information and community support, please see the Micro-Manager ...
by viktor j nilsson
Sat Sep 23, 2023 9:56 am
Forum: Camera systems and imaging
Topic: Zeiss AxioCam HRc - Worth the bother?
Replies: 30
Views: 25169

Re: Zeiss AxioCam HRc - Worth the bother?

This may be a ‘Red Herring’ but I recall looking at micro manager a few years ago There was a fairly sudden change from it being ‘open’ to the team charging for support/maintenance. … possibly aligned with the introduction of v2.0 https://micro-manager.org/Writing_plugins_for_Micro-Manager … Your e...
by viktor j nilsson
Tue Sep 05, 2023 3:48 pm
Forum: Microscopes and optics
Topic: Nikon DIC Turret Objectives
Replies: 10
Views: 4898

Re: Nikon DIC Turret Objectives

DIC marked Mplans are guaranteed to be strain free, but are otherwise identical to regular Mplans. Very few of the objectives I've tried with DIC have suffered from strain, so I would think that virtually all regular Mplans will perform perfectly fine with these prisms.
by viktor j nilsson
Wed Aug 16, 2023 6:11 pm
Forum: For forum members who want to buy and sell equipment
Topic: Leitz ICT (DIC) condenser and ICT objectives
Replies: 2
Views: 1579

Re: Leitz ICT (DIC) condenser and ICT objectives

http://www.thilo-immel-optics.de/komplettmikroskope.htm

Thilo Immel has a compete Dialux 22 with ICT. A bit pricy at €4950. But I'm afraid it won't be easy or cheap to find a ICT set for your scope.
by viktor j nilsson
Fri Aug 04, 2023 5:50 pm
Forum: Illumination Techniques
Topic: Not happy with my videos
Replies: 44
Views: 13427

Re: Not happy with my videos

Ryanb wrote:
Fri Aug 04, 2023 5:02 pm
viktor j nilsson wrote:
Tue Oct 25, 2022 7:36 am
Yes, you probably need a Facebook account.
If you pm me your email I can send you the pdf.
Would you be able to send me the PDF as well? I will send you my email if you’re willing. Thanks!!
No probs, pm your email and I'll send it
by viktor j nilsson
Mon Jun 26, 2023 5:15 am
Forum: Beginner's corner
Topic: Tutorial for using the site’s search engine?
Replies: 5
Views: 2116

Re: Tutorial for using the site’s search engine?

Use an external search engine instead.
If you search for 'Leitz condenser numerical aperture site:microbehunter.com' (without the apostrophes) on Google you'll get much more relevant hits.
by viktor j nilsson
Fri Jun 16, 2023 3:39 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB Olympus BH2 DIC Set
Replies: 72
Views: 20083

Re: WTB Olympus BH2 DIC Set

Oh yeah, same set. Weird, it was gone from the Facebook group when I checked.
by viktor j nilsson
Fri Jun 16, 2023 2:58 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB Olympus BH2 DIC Set
Replies: 72
Views: 20083

Re: WTB Olympus BH2 DIC Set

Seems to have sold quickly
by viktor j nilsson
Fri Jun 16, 2023 11:16 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB Olympus BH2 DIC Set
Replies: 72
Views: 20083

Re: WTB Olympus BH2 DIC Set

A set was just posted for sale in the Olympus microscope users group on Facebook, $3000. Including a rotating stage.
by viktor j nilsson
Fri May 26, 2023 12:02 pm
Forum: Microscopes and optics
Topic: Olympus LB versus SB objectives
Replies: 16
Views: 6065

Re: Olympus LB versus SB objectives

But the biggest disadvantage of the SB Olympus objectives, IMHO, is the too short working distance . This feature has been mentioned on the Olympus brochure. Indeed, the front lens of even the 10X0.32 planapo SB objective is very close to the coverslip. That of the 20X0.65 planapo is dangerously cl...