Search found 121 matches

by mnmyco
Mon Oct 21, 2019 9:55 pm
Forum: Microscopes and optics
Topic: Sorry, but need a quick answer
Replies: 1
Views: 58

Sorry, but need a quick answer

Does anyone know if the nosepiece, binocular, and halogen lamp socket from an AO microstar that is in their white, square time period (so like series 410 or so look) will fit a series 20? I got an amazing private offer considering what is on the microscope. I only have 30 hours or so. Again sorry fo...
by mnmyco
Mon Oct 21, 2019 3:13 am
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 121

Re: Disposing of stains from home microscopy lab

No, it does. There was a paper published on it. If I can find it again I will post a link. I refuse to stop using it. I mean I’ve had to regularly work with KCN and NaCN. If I can protect myself from exposure to those I think I can for EtBr as well.
by mnmyco
Sun Oct 20, 2019 2:42 am
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 121

Re: Disposing of stains from home microscopy lab

Oxidizing can produce even worse chemicals. For instance, bleach and ethidium bromide makes much worse chemicals than ethidium bromide. California actually allows 9 ng/L of ethidium bromide in the normal sewer system in some cities. I doubt you produce enough to cause any harm however you dispose of...
by mnmyco
Fri Oct 18, 2019 10:49 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

There are two types of hard drive disks. Some are made of aluminum and others are made of ceramic. Seems most people that cut them use a jewelers saw and some sort of cutting fluid I believe. Maybe some day I will give making a DIY water jet cutter. I know it has been done.

MNMyco
by mnmyco
Fri Oct 18, 2019 4:51 pm
Forum: Home-made microscope adaptations
Topic: Fixed TL and Infinity Inter-company Compatibly
Replies: 3
Views: 68

Re: Fixed TL and Infinity Inter-company Compatibly

You have a point, but I do not see that as a reason to not generate such a list. I was thinking of things beyond just fixed vs infinite TL. A good example is the use of 70x LOMO WI lenses without the water immersion. Or knowing if I could screw a Zeus condenser into a AO condenser and still have pha...
by mnmyco
Fri Oct 18, 2019 4:47 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

Good point. Do you have any suggestions for a good way to test that? Especially if it does not involve cutting the disks?

MNMyco
by mnmyco
Fri Oct 18, 2019 5:37 am
Forum: Illumination Techniques
Topic: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.
Replies: 15
Views: 871

Re: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.

I have ended up with two turret condenser bodies for my AO series 10 and 20 microscope. Once I get them both in working order (need another trinocular and series 20 lamp cord and socket) I will try to create a much more controlled test of this “relief phase contrast”.

MNMyco
by mnmyco
Fri Oct 18, 2019 4:45 am
Forum: Home-made microscope adaptations
Topic: Fixed TL and Infinity Inter-company Compatibly
Replies: 3
Views: 68

Fixed TL and Infinity Inter-company Compatibly

I have a small suggestion, which I have not seen a thread or website cover (please post link if I am wrong). I think it would be handy to have a list of microscope models with a list of objectives and/or other microscope accessories that are from a different manufacturer than the microscope body but...
by mnmyco
Mon Oct 14, 2019 6:01 pm
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

Re: AO-Reichert 1973A condenser DIY help request

Not sure why the image is fuzzy. It wasn’t when I posted it. I am missing a lens element though, aren’t I?
by mnmyco
Sat Oct 12, 2019 6:40 am
Forum: Beginner's corner
Topic: Fusing glass slides
Replies: 23
Views: 840

Re: Fusing glass slides

I once fused the bottom of a Pyrex jar to the top of a ceramic heater. I was converting sodium bicarbonate to sodium carbonate and forgot the thing on the highest setting. You could try putting glass water between the in slides, heat it until the water leaves creating hard silica and use a technique...
by mnmyco
Fri Oct 11, 2019 8:58 pm
Forum: Microscopes and optics
Topic: Leitz binocular head teardown
Replies: 6
Views: 393

Re: Leitz binocular head teardown

That looks like fungal damage that is not possible to repair.
by mnmyco
Fri Oct 11, 2019 8:47 pm
Forum: My microscope
Topic: DIY DIC Effect on Darkfield.... unreal
Replies: 6
Views: 587

Re: DIY DIC Effect on Darkfield.... unreal

That is basically oblique lighting from what I saw. I just toss a quarter over the light sometimes for the same thing.

MNMyco
by mnmyco
Fri Oct 11, 2019 6:07 pm
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

Re: AO-Reichert 1973A condenser DIY help request

Here is what I was sent. Very tight fit. If they are the right parts they are not from the same housing as the one that was sent. Thanks for the info on the polarizing condenser. I figured that is was red meant. Interestingly, that condenser has the notch to fit on the fork of my series 10 and 20. I...
by mnmyco
Fri Oct 11, 2019 5:39 am
Forum: Identification help
Topic: Amoeboid infected with parasites?
Replies: 7
Views: 575

Re: Amoeboid infected with parasites?

I would guess it is food being digested via phagocytosis.

MNMyco
by mnmyco
Fri Oct 11, 2019 4:58 am
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

Re: AO-Reichert 1973A condenser DIY help request

Finally arrived today. Seller included the bottom cell of elements, but it is still missing the middle cell. If the 1973A shares the same number and layout as the 1973. Apo, if possible, could you post a photo of the separate parts of the condenser? I would like to show the seller In case he does ha...
by mnmyco
Tue Oct 08, 2019 5:34 pm
Forum: Microscopes and optics
Topic: Polarizing filter delamination?
Replies: 8
Views: 503

Re: Polarizing filter delamination?

There are fairly inexpensive glass polarizers on eBay. For example SVBONY. Diameter 25mm and probably other sizes too. They are sold installed in metal sleeves but the sleeve can be easily dismantled to extract the glass disc. Would'nt it be possible to replace the delaminated piece in the condense...
by mnmyco
Tue Oct 08, 2019 5:52 am
Forum: Specimens, samples and slides
Topic: Tried glycerin to slow down fast movers
Replies: 36
Views: 5136

Re: Tried glycerin to slow down fast movers

For methylcellulose, bring water to near boil and add in metheylcellulose. Do this while mixing and mix rapidly until no large clumps can be see. This prevents clumping as methylcellulose is actually insoluble at higher temps. Cool with rapid, constant stirring and you will get a nice solution. Meth...
by mnmyco
Tue Oct 08, 2019 5:46 am
Forum: Microscopes and optics
Topic: Polarizing filter delamination?
Replies: 8
Views: 503

Re: Polarizing filter delamination?

Just got it apart. It is in between two glass disks and that is where it has become separated from both. I think this means it should work. Has anyone worked on an issue like this and have advice?
by mnmyco
Tue Oct 08, 2019 3:11 am
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

Re: AO-Reichert 1973A condenser DIY help request

I am a bit unclear on what was sent, but from the sellers email is sounds like he sent everything. Apparently, they have 20+ pieces, but are not all complete and in unknown condition. So, they don’t advertise them as complete. The image shows the housing and the top lens set in the brass piece. We w...
by mnmyco
Tue Oct 08, 2019 3:02 am
Forum: Microscopy accessories
Topic: Olympus equipment ID request
Replies: 2
Views: 360

Re: Olympus equipment ID request

Here are pictures you can just see. https://hosting.photobucket.com/images/aa169/Mnmmyco/0/8b5ba3df-c00f-466d-aa09-944a690f900d-original.jpg?width=1920&height=1080&fit=bounds https://hosting.photobucket.com/images/aa169/Mnmmyco/0/908761fa-a5a3-415f-a7cc-7163044bb2f5-original.jpg?width=1920&height=10...
by mnmyco
Tue Oct 08, 2019 2:44 am
Forum: Microscopes and optics
Topic: Polarizing filter delamination?
Replies: 8
Views: 503

Polarizing filter delamination?

I recently purchased a AO polarizing condenser, but the filter looks a bit odd.

Image

Is this an issue?

MNMyco
by mnmyco
Sun Oct 06, 2019 11:36 pm
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

Re: AO-Reichert 1973A condenser DIY help request

I have purchased a 1201 as well, but the seller did email me and let me know he included what is believed to be the other lens for the 1973A. Once I get it I will post here and let you know what materials I received and what you think I can do with those. I am unclear if all the pieces, besides the ...
by mnmyco
Sun Oct 06, 2019 11:34 pm
Forum: Beginner's corner
Topic: minimizing harm
Replies: 61
Views: 7509

Re: minimizing harm

UV is inherently deadly to life. Keep in mind that microbes in nature can seek the depths to avoid UV as well as seek what shade there is. Neither are a realistic options to microbes on a slide. UV will always cause damage to DNA and the ability to repair that damage depends on the species we are ta...
by mnmyco
Sat Oct 05, 2019 12:38 am
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 12
Views: 756

AO-Reichert 1973A condenser DIY help request

I have managed to find the housing and top lens for a 1973A 1.4 NA achromatic condenser. I know that Apo has made a DIY version with these pieces in the past. So, I am soliciting help and advice for attempting some sort of rebuild. I’m a bit l lazy, but if anyone is interested I will find the eBay l...
by mnmyco
Sat Oct 05, 2019 12:30 am
Forum: Beginner's corner
Topic: minimizing harm
Replies: 61
Views: 7509

Re: minimizing harm

Sorry if this is a repeat, but you can block most or all UV with just some clear plastic filters. Lexan will basically block all UV light, though IR will be transmitted. http://www.plasticgenius.com/2011/05/infrared-and-ultraviolet-transmission.html https://www.acplasticsinc.com/informationcenter/r/...
by mnmyco
Sat Oct 05, 2019 12:17 am
Forum: Pictures and Videos
Topic: Peranema eating a dead rotifer
Replies: 9
Views: 642

Re: Peranema eating a dead rotifer

You should try to stain the rotifers with a very weak solution of Congo red and then feed it to the Peranema. You should be able to watch the color change due to the pH. I have down this with yeast and paramecium for an intro bio lab. The students didn’t see much though as they a rather poor at usin...
by mnmyco
Tue Oct 01, 2019 7:55 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

I found this website about using hard drive platters in laser as mirrors. In case anyone is interested to read it I posted it here. I think I will try cutting some soon.

http://www.imajeenyus.com/optical/20140 ... ndex.shtml

MNMyco
by mnmyco
Tue Oct 01, 2019 7:50 pm
Forum: For forum members who want to buy and sell equipment
Topic: Bausch & Lomb Type H, 1932-3...SOLD....
Replies: 4
Views: 1163

Re: Bausch & Lomb Type H, 1932-3...SOLD....

The B & L is in great condition. I hope it ends up in something better than a display cabinet.

MNMyco
by mnmyco
Sun Sep 29, 2019 3:26 am
Forum: Pictures and Videos
Topic: A Mixture of Things Within Moss
Replies: 9
Views: 255

Re: A Mixture of Things Within Moss

Keep an eye out for tardigrades in your moss.