Search found 100 matches

by RobBerdan
Sun Sep 06, 2020 5:58 pm
Forum: Pictures and Videos
Topic: Copepods
Replies: 3
Views: 139

Copepods

Below are some images of Copepods - there are 3 main types 1) Calanoida 2) Cyclopoida and 3) Harpacticoida - I am still trying to find some Harpacticoids. The Calanoida have very long antenna, but most of the time when mounted on a slide they turn onto their side despite my efforts, finally decided ...
by RobBerdan
Fri Sep 04, 2020 9:08 pm
Forum: Pictures and Videos
Topic: Rotifers from the summer of 2020
Replies: 5
Views: 204

Re: Rotifers from the summer of 2020

Hi Don - in my article about cameras for photomicrography I show some of my microscopes - some new and some old. https://www.canadiannaturephotographer.com/cellphones_dedicatedcameras_DSLRs.html I have been taking pictures with microscopes for a long time (50 years), but only in the past few years h...
by RobBerdan
Fri Sep 04, 2020 4:14 pm
Forum: Pictures and Videos
Topic: Rotifers from the summer of 2020
Replies: 5
Views: 204

Rotifers from the summer of 2020

I love photographing rotifers as they are just the right size for light microscopy, not too small and not too big. There are 2149 species so unlike ciliates their identification is a bit easier. Some rotifers can move quickly my solution is to compress them slightly by drawing water with piece of pa...
by RobBerdan
Wed Sep 02, 2020 10:40 pm
Forum: Pictures and Videos
Topic: Programmed nuclear death in Paramecium
Replies: 17
Views: 498

Re: Programmed nuclear death in Paramecium

Beautiful fluorescent images of Paramecium - bravo.
Cheers
RB
by RobBerdan
Fri Aug 28, 2020 4:40 pm
Forum: Pictures and Videos
Topic: The best Camera for Photomicrography
Replies: 24
Views: 1068

Re: The best Camera for Photomicrography

RE - Diatom colour shift - that is my fault. I shoot RAW files and when I process the images I must have used a slightly different setting, or I may have twisted the DIC compensator slightly between taking the pictures which changes the background colour. Sometimes I see colour fringing, not sure if...
by RobBerdan
Fri Aug 28, 2020 4:36 pm
Forum: Pictures and Videos
Topic: The best Camera for Photomicrography
Replies: 24
Views: 1068

Re: The best Camera for Photomicrography

Thanks for your comments I will try to address your questions Questions 1. Citation: "The AM microscope camera ... is shown above with an eyepiece attached to it that inserts into an ocular, but the camera can connect directly to a C mount. All the cameras can use these eyepieces for insertion into ...
by RobBerdan
Fri Aug 28, 2020 2:05 am
Forum: Pictures and Videos
Topic: The best Camera for Photomicrography
Replies: 24
Views: 1068

The best Camera for Photomicrography

I have posted an article on my web site where my son and I tested 4 different dedicated cameras for photomicrography 1) AMscope 3MP 2) Zeiss Axiocam 5MP 3) Moticam 5.5 MP and 4) Rising Cam 20 MP camera. We also tested 3 Cells phones a) Iphones 5s b) Samsung Galaxy IIIS and c) Google Pixel 3. These w...
by RobBerdan
Sun Aug 09, 2020 4:12 am
Forum: Camera systems and imaging
Topic: For fluorescence microscopy, specialized microscope camera or DSLR?
Replies: 8
Views: 5202

Re: For fluorescence microscopy, specialized microscope camera or DSLR?

I realize this post is over a year old, but I feel compelled to answer it. I own a Fluorescence microscope Nikon Optiphot as well several other scopes. I have been testing different models of dedicated cameras including Zeiss, AM systems, Moticom and Rising Cam. The Rising cam offers 20 MP and is th...
by RobBerdan
Thu Aug 06, 2020 4:09 am
Forum: Pictures and Videos
Topic: Diatom 640X Oblique Illumination
Replies: 24
Views: 4138

Re: Diatom 640X Oblique Illumination

I am fortunate to own a DIC microscope, it costs a lot, but after many years I just decided I wanted to own a scope with this technique and I use it frequently, but its cost > $10,000 which puts it out of reach of many. I have seen some really good Oblique lighting photos and I have played with the ...
by RobBerdan
Fri Jul 03, 2020 2:01 am
Forum: Pictures and Videos
Topic: Cyanobacteria in Freshwater ponds
Replies: 6
Views: 648

Cyanobacteria in Freshwater ponds

Cyanobacteria used to be called blue-green algae, but they are related to bacteria - procaryotes which lack a nucleus and fossil evidence suggests they have been around for over 3 billion years. They produced the earth's first oxygen. Some species are able to produce potent toxins. I have always bee...
by RobBerdan
Tue Jun 09, 2020 2:01 am
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 19
Views: 1596

Re: Fresh water diatoms

Very nice crisp DIC images Charles showing lots of internal detail.
Cheers
RB
by RobBerdan
Mon Jun 01, 2020 3:35 am
Forum: Pictures and Videos
Topic: Rheinberg filters and other Techniques
Replies: 4
Views: 1162

Rheinberg filters and other Techniques

Rheinberg filters have been around a long time. You can make them your self or purchase a set on E-bay for about $30. These simple filters can add colour contrast with almost any microscope. I am attaching some photos using a variety of techniques, polarization, bright field, dark-field, DIC, Rheinb...
by RobBerdan
Wed May 27, 2020 8:15 pm
Forum: Pictures and Videos
Topic: How to do Polarization Microscopy
Replies: 7
Views: 602

Re: How to do Polarization Microscopy

Hi I watched your video, it is very well done with lots of good information. Videos take a lot of time in my experience though I hope to be doing more in the future myself. I like how you made the microscope cover come off :-) One of the things in polarization microscopy that can really enhance the ...
by RobBerdan
Wed May 27, 2020 7:42 pm
Forum: Pictures and Videos
Topic: Chaoborus - Phantom Midge Fly Larva
Replies: 4
Views: 393

Chaoborus - Phantom Midge Fly Larva

A few weeks ago I posted some images of Chaoborus the phantom midge fly larva also called a Glassworm because of its transparency. I continued to photograph the pupa, its prey and made a movie of rotifers living on the Chaoborus. I just posted an article about the biology of this organism along with...
by RobBerdan
Thu May 14, 2020 2:21 am
Forum: Pictures and Videos
Topic: Fragilaria
Replies: 13
Views: 947

Re: Fragilaria

Very high quality images - nice job!
RB
by RobBerdan
Thu May 14, 2020 2:19 am
Forum: Pictures and Videos
Topic: Is This Algae or a Worm?
Replies: 4
Views: 939

Re: Is This Algae or a Worm?

I smiled when I saw your question. I agree they are blue-green algae also called cyano-bacteria. Oscillatoria is most likely the genus. I see a variety of motile blue green algae in pond water often and it is fascinating to watch. See https://en.wikipedia.org/wiki/Oscillatoria on Wikipedia. I think ...
by RobBerdan
Tue May 12, 2020 12:55 am
Forum: Pictures and Videos
Topic: Arachnoidiscus
Replies: 16
Views: 1720

Re: Arachnoidiscus

Beautiful detail in the Diatom - thanks for sharing.
RB
by RobBerdan
Mon May 11, 2020 7:59 pm
Forum: Pictures and Videos
Topic: Mothers Day Pond Collecting
Replies: 0
Views: 558

Mothers Day Pond Collecting

Since the pond ice melted 2 weeks ago, I have been visiting the ponds each weekend and collecting protists, water beetles and anything else that is interesting. I hope to keep this up throughout the summer as the abundance of organisms changes drastically even over one week as does the species that ...
by RobBerdan
Mon May 11, 2020 7:37 pm
Forum: Pictures and Videos
Topic: spring'2020 bear hunt with questions'.
Replies: 27
Views: 2531

Re: spring'2020 bear hunt with questions'.

Hi - I have only found a few water bears so far this year collected from lichen growing on a mountain ash tree in my yard. I have found two species living there and also found the epizoic peritrich ciliates growing on some and show pictures of Pyxidium tardigradum growing on Ramazzottius oberhaeuser...
by RobBerdan
Tue May 05, 2020 3:17 am
Forum: Pictures and Videos
Topic: Phantom Midge Chaoborus crystallinus
Replies: 8
Views: 859

Re: Phantom Midge Chaoborus crystallinus

All the specimens are just in pond water with a coverlip on top.
Cheers
RB
by RobBerdan
Tue May 05, 2020 2:50 am
Forum: Microscopy accessories
Topic: The most important microscope accessory
Replies: 11
Views: 2436

Re: The most important microscope accessory

Ah .... but what is the second most important microscope accessory? Could it be a microscope cover? Could it be a anti-vibration padding - I use 1\4 inch rubber matts used in gyms that I buy at the hardware store and cut to size. Could it be a very bright even light source for the microscope, I used...
by RobBerdan
Tue May 05, 2020 2:16 am
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 12
Views: 2098

Re: Epi-Fluorescence Microscopy and Deconvolution

Greg Benz describes a technique for Deconvolution sharpening in Camera RAW. What I do is open the image in Photoshop Camera Raw and adjust the Black, clarity, Haze an texture sliders to improve clarity. I reduce the luminance noise to reduce grain. I may adjust the vibrance, and saturation a little ...
by RobBerdan
Mon May 04, 2020 8:35 pm
Forum: Pictures and Videos
Topic: Vaginicola
Replies: 10
Views: 1511

Re: Vaginicola

Nice video using DIC microscopy.
Cheers
RB
by RobBerdan
Mon May 04, 2020 8:33 pm
Forum: Pictures and Videos
Topic: Wierd behaivor from unknown microorganism
Replies: 6
Views: 1084

Re: Wierd behaivor from unknown microorganism

HI I agree the organism is too small in the video - with a 63X objective you should be able to zoom in more. Also try hold your cell phone side ways, and if you would like to do more consider attaching a digital camera either a dedicated microscope camera eg. form AMscopes.com or get a phone holder ...
by RobBerdan
Mon May 04, 2020 8:30 pm
Forum: Pictures and Videos
Topic: Actinocyclus subtilis
Replies: 18
Views: 1544

Re: Actinocyclus subtilis

Very nice DIC images!
RB
by RobBerdan
Mon May 04, 2020 8:27 pm
Forum: Pictures and Videos
Topic: Phantom Midge Chaoborus crystallinus
Replies: 8
Views: 859

Phantom Midge Chaoborus crystallinus

The phantom midge fly larva is a fierce predator of Daphnia, Copepods and Ciliates found in freshwater ponds. I first encountered them this weekend and tried a number of different photographic techniques. Because of their large size I needed to make panoramas to capture the entire organism. I also t...
by RobBerdan
Mon May 04, 2020 8:15 pm
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 12
Views: 2098

Re: Epi-Fluorescence Microscopy and Deconvolution

I have been testing different deconvolution programs, some of them very expensive and so far I have been able to do as good, sometimes better using Photoshop. I have added some comparisions to my article. I also found that Nikon NIS elements offers a web site where you can drag and drop a .TIF file,...
by RobBerdan
Thu Apr 30, 2020 3:11 am
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 12
Views: 2098

Re: Epi-Fluorescence Microscopy and Deconvolution

Hi to do deconvolution in Image J you need to get a plug in and download - here is the link, read how to install the plugin -it is straight forward - https://imagej.net/Deconvolution I have only seen deconvolution used for fluorescence and confocal microscopy. On photomacrography.net Rogelio Moreno ...
by RobBerdan
Tue Apr 28, 2020 5:56 pm
Forum: Pictures and Videos
Topic: First Photo of a Microbe
Replies: 8
Views: 1231

Re: First Photo of a Microbe

HI - it looks like Chydorus sp , not a Daphnid - Nice photo. There are lots of books on Freshwater aquatic organisms with keys on the web for free and also for sale. Some references online (1959) W.T. Edmonson (ed) Fresh Water Biology 2nd edition, John Wiley. NY pg 420-507 - read online version free...
by RobBerdan
Tue Apr 28, 2020 5:49 pm
Forum: Pictures and Videos
Topic: Micro stonemasons. Difflugia.
Replies: 21
Views: 2584

Re: Micro stonemasons. Difflugia.

Very nice photos.
RB