Search found 42 matches

by RobBerdan
Mon Sep 02, 2019 8:36 pm
Forum: Pictures and Videos
Topic: Micro-Universe
Replies: 4
Views: 630

Micro-Universe

Can't believe summer is over and I only had a few opportunities to photograph my favourite subject - Pond Life. I just posted two articles 1) The Micro-Universe and 2) Microscopic Pond Life for those that might be interested: 1. https://www.canadiannaturephotographer.com/microuniverse.html 2. https:...
by RobBerdan
Mon May 13, 2019 2:39 am
Forum: Pictures and Videos
Topic: Micro-Universe Presentation
Replies: 4
Views: 1456

Micro-Universe Presentation

I am giving a presentation at a public library to try and get more folks interested in microscopy, I am attaching a couple of the images I am going show in the presentation. I also have a series of movies to show pond organisms, ciliates, rotifers, amoeba etc. I also have a picture that kids took wi...
by RobBerdan
Sun Apr 21, 2019 10:40 pm
Forum: Pictures and Videos
Topic: Ascorbic Acid eyes
Replies: 4
Views: 3125

Re: Ascorbic Acid eyes

Definintely Vitamin C - I see lots of similar crystals like this - very beautiful.
RB
by RobBerdan
Mon Apr 15, 2019 3:26 am
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4298

Re: Tips on Buying a Microscope and suggestions for What to look at

The LEDs I got were 20W Cree from Stanton on Ebay, not exactly sure what the colour temperature is - looks like about 6500K, but unlike tungsten which are warm 3200-3400 K, these LEDs seem almost blue in colour. I still colour correct the background so its a neutral white using RGB numbers in Photos...
by RobBerdan
Sun Apr 14, 2019 3:29 am
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4298

Re: Tips on Buying a Microscope and suggestions for What to look at

I am not sure I have answer for your question about kids. I think they need to see something compelling. I am just starting to do some presentations - I will try to include some movies with music so they can see microorganisms moving like water bears and paramecium. I will also bring a microscope so...
by RobBerdan
Sun Apr 14, 2019 12:05 am
Forum: Microscopy accessories
Topic: Rheinberg Filters
Replies: 7
Views: 7532

Re: Rheinberg Filters

I have purchased a set of the Rheinberg filters on ebay for $30 and I think they work great! I wrote an article on Rheinberg filters: https://www.canadiannaturephotographer.com/rberdan_Rheinberg_filters2017.html And I am including one photo of a ribbon worm below I took with a Rheinberg filters. I a...
by RobBerdan
Sat Apr 13, 2019 11:38 pm
Forum: Illumination Techniques
Topic: Bright field, Darkfield, Phase Contrast and Rheinberg compared
Replies: 2
Views: 4142

Bright field, Darkfield, Phase Contrast and Rheinberg compared

Below are pictures from a recent article I posted on my web site showing different lighting techniques. Photos are of Wood by auto - fluorescence, Volvox by bright field, dark field, positive phase contrast, and Ribbon worm Rheinberg. Rheinberg filter set can be purchased on Ebay for about $30. Furt...
by RobBerdan
Sat Apr 13, 2019 11:17 pm
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4298

Tips on Buying a Microscope and suggestions for What to look at

I just posted an article on my web site including tips for buying a new and used microscope, also why some one might want to own a microscope and some subjects they can look at. https://www.canadiannaturephotographer.com/guide_buying_microscope.html Comments welcome Photos include, rotifer, onion ro...
by RobBerdan
Sat Apr 13, 2019 9:30 pm
Forum: Beginner's corner
Topic: Tips for Buying a Light Microscope
Replies: 2
Views: 3483

Tips for Buying a Light Microscope

Tips for Buying a Light Microscope - Compound, Inverted and Stereoscope and Why you might want to buy a Microscope I have just posted an article on my web site about Tips for buying a new or used microscope including stereoscopes, compound microscopes and inverted microscopes. I show some specimens...
by RobBerdan
Thu Mar 28, 2019 9:49 pm
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2032

Re: Crystal Mania

Hi Radazz - in answer to your question. Usually the crystals are bigger when they are allowed to form overnight of over a period of days. Slow growth seems to result in bigger crystals. I sometimes accelerate evaporation by heating the slides on a hot plate, but the crystals tend to be larger when d...
by RobBerdan
Thu Mar 28, 2019 9:39 pm
Forum: Pictures and Videos
Topic: Movie with Music showing Vitamin C crystals in Polarized Ligh
Replies: 3
Views: 1368

Movie with Music showing Vitamin C crystals in Polarized Ligh

I used Windows Movie Maker to create a short 3 minute slide show with music showing some of my favourite Crystals viewed with Polarizing microscopy. You can view the movie on Youtube here: https://www.youtube.com/watch?v=18DIc8BpRoA&feature=youtu.be If you have a fast connection you can view or down...
by RobBerdan
Tue Mar 26, 2019 5:44 pm
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2032

Re: Crystal Mania Questions

To answer your question how much Vitamin C to use - I generally make up a tube of 3 ml of deionized water, and 3 ml of 99% isopropyl alcohol - and add about small teaspoon of the powder and mix thouroughly - I use a laboratory vortex mixer but shaking would be fine to get as much of the powder disso...
by RobBerdan
Mon Mar 25, 2019 2:06 am
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2032

Crystal Mania

I am working on photographing crystals with polarized light and trying to optimize conditions for growing Vitamin C crystals which in my opinion are some of the most beautiful crystals I have seen. A couple of things I have learned is that a mix of water and isopropyl alcohol seems to work best. Iso...
by RobBerdan
Mon Mar 25, 2019 1:47 am
Forum: Pictures and Videos
Topic: Rotifer from Hamburg Alster - Brachionus calyciflorus?
Replies: 5
Views: 1344

Re: Rotifer from Hamburg Alster

Very nice looks like you used Bright field and it worked very well.
RB
by RobBerdan
Tue Mar 19, 2019 10:42 pm
Forum: Pictures and Videos
Topic: Article about Crystals in Polarized light and by DIC
Replies: 9
Views: 2237

Re: Article about Crystals in Polarized light and by DIC

Thank you for your kind comments. I submitted an article to Micscape a few months ago and it can be found here: http://www.microscopy-uk.org.uk/mag/artoct18/rb-Photomicro-Science-Art.pdf - its about Science & Art. I am trying to promote microscopy in Canada and possibly start a microscopy club in Ca...
by RobBerdan
Mon Mar 18, 2019 12:49 am
Forum: Pictures and Videos
Topic: Article about Crystals in Polarized light and by DIC
Replies: 9
Views: 2237

Article about Crystals in Polarized light and by DIC

Dear microscopists - in an earlier post I mentioned I was working on an article for my web site about crystals in polarized light and by DIC. I have photographed, Vitamins, beer, alcohol, drugs and even human tears. I share my methods I used to produce the crystals which I hope will be of interest t...
by RobBerdan
Tue Mar 12, 2019 1:09 am
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1276

Re: Crystals and Polarized light

That is correct, however the sodium chloride induces crystals which can be seen and photographed easily with DIC. They are not birefringent, but somehow stimulate crystal formation where they would not otherwise form. Below these are Corona extra beer crystals formed in the presence of salt - otherw...
by RobBerdan
Mon Mar 11, 2019 6:52 pm
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1276

Re: Crystals and Polarized light

Thanks Bryan - I noticed you added table salt - it turns out that every alcoholic drink I add table salt do also crystallizes. So I tried NaOH no crystals. I tried NaCl (table salt) to, water, methanol, and ispropanol - no crystals suggesting it is the Cl ion that might be important or something in ...
by RobBerdan
Mon Mar 11, 2019 4:32 pm
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1276

Crystals and Polarized light

I have been experimenting with different drinks, beer and chemicals using polarized light and the technique produces amazing colors and patterns. The Microbe hunter owner provides a nice video on how to convert any microscope to a polarizing microscope for only a few dollars: https://www.youtube.com...
by RobBerdan
Mon Feb 25, 2019 11:42 pm
Forum: Pictures and Videos
Topic: Crystals under Polarized Light and DIC
Replies: 8
Views: 1631

Re: Crystals under Polarized Light and DIC

Yes the last crystals in the last picture are strontium. The beer crystals, I am not sure - they could be sugar, starch or protein - getting beer to crystallize has been difficult. I am going to try some precipitants to see if that might help promote crystallization. I have been boiling the beer to ...
by RobBerdan
Mon Feb 25, 2019 12:14 am
Forum: Pictures and Videos
Topic: Crystals under Polarized Light and DIC
Replies: 8
Views: 1631

Crystals under Polarized Light and DIC

Most crystals are pretty easy to prepare for photomicrography, either melt them on the slide, or dry them from a solution of water or alcohol - a coverslip isn't necessary, but helps keep the crystals flat. Proteins and other substances e.g. beer are more difficult to crystallize. I have been trying...
by RobBerdan
Fri Jan 04, 2019 8:36 pm
Forum: Pictures and Videos
Topic: Diatom Samples from the Netherlands.
Replies: 11
Views: 1704

Re: Diatom Samples from the Netherlands.

Hi Jochen - I used a full 550 nm wave plate (full lambda plate) and when I turn the Zeiss DIC prisms above the objective I can achieve different coloured backgrounds with DIC. The wave plate or a quartz wedge also works with a polarizing microscope as you probably already know. I have found that mic...
by RobBerdan
Fri Jan 04, 2019 5:25 pm
Forum: Pictures and Videos
Topic: Diatom Samples from the Netherlands.
Replies: 11
Views: 1704

Re: Diatom Samples from the Netherlands.

Hi - to answer your questions - I am confident of the resolution of the raphe being 0.9 microns with 40X dry objective. I determine the magnification on the digital image using a micrometer slide and eyepiece ocular to measure the length of the Diatom and and then I use Photoshop to measure the numb...
by RobBerdan
Thu Jan 03, 2019 11:49 pm
Forum: Pictures and Videos
Topic: Diatom Samples from the Netherlands.
Replies: 11
Views: 1704

Diatom Samples from the Netherlands.

I just received some cleaned Diatoms in a small tube that I ordered from the Netherlands for 10 euros per tube. I ordered them from their web site: https://www.diatoms.nl/search.html?search%5Bkeywords%5D=diatoms&_a=category&token=e5023feb8eb5f63a83aba54178ee6bd9 I ordered both samples that they offe...
by RobBerdan
Sat Dec 29, 2018 11:14 pm
Forum: Pictures and Videos
Topic: Birefringence of Ice
Replies: 10
Views: 1244

Re: Birefringence of Ice

Thank you for kind words. I hope to try some more of this type of photography and also see if I can photograph the birefringence in snowflakes this winter. I will post the photos and\or article later this winter.
by RobBerdan
Sat Dec 29, 2018 3:39 am
Forum: Pictures and Videos
Topic: Birefringence of Ice
Replies: 10
Views: 1244

Birefringence of Ice

It's pretty cold outside here in Calgary, AB and I had read that ice is birefringent (anistropic) or has 2 or more refractive indices which result in different colours when observed through a microscope using a polarizer and analyzer. A full wave plate can also enhance the colours. I took several mi...
by RobBerdan
Sun Dec 23, 2018 2:36 am
Forum: Digital processing
Topic: Stacking Software
Replies: 35
Views: 12875

Re: Stacking Software Tested

I tested Zerene, Helicon Focus and Photoshop for photomicrography. Helicon performed best and I could use it with RAW files. I still use Photoshop for quick tests with my RAW files and it also works well most of the time. See my test pictures and article here if interested : https://www.canadiannatu...
by RobBerdan
Sat Dec 22, 2018 11:42 pm
Forum: For forum members who want to buy and sell equipment
Topic: for sale camera adapter
Replies: 3
Views: 2206

Re: for sale camera adapter

I am interested in the Olympus adapter both parts and the FK 3.3X eyepiece. I have two of them already but own three Olympus microscopes. If the cost is reasonable I might purchase them. I am located in Calgary, AB so would also need to know the shipping cost from your place to mine in Calgary. What...
by RobBerdan
Sun Dec 09, 2018 10:11 pm
Forum: My microscope
Topic: My Zeiss Microscopes
Replies: 76
Views: 26428

Re: My Zeiss Microscopes

Hi Jochen - I saw your pictures of mini-aquarium slides. I use petri dishes with coverslip bottoms with my inverted microscope. I don't have picture of all my microscopes but will include a photos of some of them. I have an Olympus E with phase contrast, Olympus IM inverted I purchased recently off ...
by RobBerdan
Sun Dec 09, 2018 9:25 pm
Forum: Illumination Techniques
Topic: How is this not DIC? Oblique illumination? HOW?
Replies: 10
Views: 3733

Re: How is this not DIC? Oblique illumination? HOW?

Oblique illumination can often be achieved by simplying having a filter tray under the condenser partlially blocking the light, one can also use various shaped filters below the condenser - these filters can be made with a sheet of glass and black tape or other opaque material cut in various shapes....