Search found 53 matches

by RobBerdan
Wed Jan 22, 2020 4:39 pm
Forum: Pictures and Videos
Topic: The Microscopic Beauty of Plants & Tree
Replies: 9
Views: 201

Re: The Microscopic Beauty of Plants & Tree

In answer to some questions. The branch was cut fresh, clamped and sectioned as is no fixing or softening as I did in the past. Softening the tissue by boiling in 10% glycerol and water seems to cause the bark to separate. The microtome cost me $300 US on Ebay, but I have never seen another one like...
by RobBerdan
Wed Jan 22, 2020 5:43 am
Forum: Pictures and Videos
Topic: The Microscopic Beauty of Plants & Tree
Replies: 9
Views: 201

Re: The Microscopic Beauty of Plants & Tree

Hi Thanks the web site is amazing and has beautiful photomicrographs. Today I tried an older AO Hand microtome from 1950s I purchased on Ebay and a new disposable microtome blade from India and right away I was able to cut sections between 10-15 microns - I might not need to buy a sliding microtome ...
by RobBerdan
Tue Jan 21, 2020 1:55 am
Forum: Pictures and Videos
Topic: The Microscopic Beauty of Plants & Tree
Replies: 9
Views: 201

Re: The Microscopic Beauty of Plants & Tree

Thanks for the suggestions I had a look at the device you made out of plastic I will try something similar. You say you make 7- mcron sections - I would like to make 1015 micron sections so I am looking at purchasing a sliding microtome. You have some very nice sections - I still have a lot to learn...
by RobBerdan
Mon Jan 20, 2020 5:56 am
Forum: Illumination Techniques
Topic: Bertrand Lens
Replies: 1
Views: 1839

Re: Bertrand Lens

I have a Bertrand lens in my Unitron Polarizing microscope. To use it I need to use the 40X objective- doesn't work with the other powers. The easiest sample for testing is to put a thin slice of mica where the slide would go. You may have to remove the eye piece to see the interference pattern and ...
by RobBerdan
Mon Jan 20, 2020 5:39 am
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 33
Views: 5183

Re: dIY DIC

I have DIC, Hoffman and I used oblique illumination which the article refers to. I believe the author has now purchased DIC. DIC is unfortanetly very expensive to make. A number of researchers have been working on developing software that is supposed to simulate DIC or Phase contrast, but none has a...
by RobBerdan
Mon Jan 20, 2020 5:27 am
Forum: Illumination Techniques
Topic: Rheinberg Filters
Replies: 8
Views: 3725

Re: Rheinberg Filters

The Rheinberg filters on Ebay are excellent I have purchased and used them on several of my microscopes. They cost about $30 which is cheap in my opinion. You do need to adjust the height of the filters under the condenser for different objectives. What I did with my research microscope is create a ...
by RobBerdan
Mon Jan 20, 2020 5:18 am
Forum: Illumination Techniques
Topic: why are my photo's and video's always yellowish?
Replies: 7
Views: 186

Re: why are my photo's and video's always yellowish?

Re yellow photos - you can do one of four things to fix the background colour: 1) use a blue filter - they are sometimes called daylight filters, and use the max brightness of your bulbs 2) You can set you camera white balance to tungsten about 3200 K 3) You can fix the colour quickly if you use Pho...
by RobBerdan
Mon Jan 20, 2020 5:10 am
Forum: Digital processing
Topic: Stacking Software
Replies: 36
Views: 13750

Re: Stacking Software

HI I tested all three programs I use Photoshop regularly, Helicon was better and both support RAW files. Zerene was Ok, but did not support RAW images. https://www.canadiannaturephotographer. ... cking.html
by RobBerdan
Mon Jan 20, 2020 5:06 am
Forum: Digital processing
Topic: Digitizing 35mm micro photography tranparency images
Replies: 7
Views: 1034

Re: Digitizing 35mm micro photography tranparency images

Hi - I have digitized lots of slides for clients using a Nikon coolscan and the Epson V600. The coolsan offers the highest quality, but a full res scan can take 5 minutes. The Epson will scan up to 6 images simulataneously and does a very good job, not as good as the Cools scan but good enough for m...
by RobBerdan
Mon Jan 20, 2020 4:52 am
Forum: Digital processing
Topic: Photoshop CS6 v Helicon v Zerene,and the rest
Replies: 22
Views: 2548

Re: Photoshop CS6 v Helicon v Zerene,and the rest

Hi I focus stack images regularly and compared Photoshop, Helicon and Zerene and posted my result on my web site. I use Photoshop for quick testing and it is often all I need. Helicon is better and offers more features for blending. I work with RAW files which Zerene did not support at time I did my...
by RobBerdan
Mon Jan 20, 2020 4:33 am
Forum: Pictures and Videos
Topic: The Microscopic Beauty of Plants & Tree
Replies: 9
Views: 201

The Microscopic Beauty of Plants & Tree

I just posted an article on my web site that I think some members might be interested in called the The Microscopic Beauty of Plants and Trees. I have been hand sectioning plants and trees and tried a wide variety of disposable razor blades, shaving blades and microtome blades. I am using hand micro...
by RobBerdan
Mon Sep 02, 2019 8:36 pm
Forum: Pictures and Videos
Topic: Micro-Universe
Replies: 4
Views: 715

Micro-Universe

Can't believe summer is over and I only had a few opportunities to photograph my favourite subject - Pond Life. I just posted two articles 1) The Micro-Universe and 2) Microscopic Pond Life for those that might be interested: 1. https://www.canadiannaturephotographer.com/microuniverse.html 2. https:...
by RobBerdan
Mon May 13, 2019 2:39 am
Forum: Pictures and Videos
Topic: Micro-Universe Presentation
Replies: 4
Views: 1508

Micro-Universe Presentation

I am giving a presentation at a public library to try and get more folks interested in microscopy, I am attaching a couple of the images I am going show in the presentation. I also have a series of movies to show pond organisms, ciliates, rotifers, amoeba etc. I also have a picture that kids took wi...
by RobBerdan
Sun Apr 21, 2019 10:40 pm
Forum: Pictures and Videos
Topic: Ascorbic Acid eyes
Replies: 4
Views: 3168

Re: Ascorbic Acid eyes

Definintely Vitamin C - I see lots of similar crystals like this - very beautiful.
RB
by RobBerdan
Mon Apr 15, 2019 3:26 am
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4450

Re: Tips on Buying a Microscope and suggestions for What to look at

The LEDs I got were 20W Cree from Stanton on Ebay, not exactly sure what the colour temperature is - looks like about 6500K, but unlike tungsten which are warm 3200-3400 K, these LEDs seem almost blue in colour. I still colour correct the background so its a neutral white using RGB numbers in Photos...
by RobBerdan
Sun Apr 14, 2019 3:29 am
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4450

Re: Tips on Buying a Microscope and suggestions for What to look at

I am not sure I have answer for your question about kids. I think they need to see something compelling. I am just starting to do some presentations - I will try to include some movies with music so they can see microorganisms moving like water bears and paramecium. I will also bring a microscope so...
by RobBerdan
Sun Apr 14, 2019 12:05 am
Forum: Microscopy accessories
Topic: Rheinberg Filters
Replies: 7
Views: 7824

Re: Rheinberg Filters

I have purchased a set of the Rheinberg filters on ebay for $30 and I think they work great! I wrote an article on Rheinberg filters: https://www.canadiannaturephotographer.com/rberdan_Rheinberg_filters2017.html And I am including one photo of a ribbon worm below I took with a Rheinberg filters. I a...
by RobBerdan
Sat Apr 13, 2019 11:38 pm
Forum: Illumination Techniques
Topic: Bright field, Darkfield, Phase Contrast and Rheinberg compared
Replies: 2
Views: 4364

Bright field, Darkfield, Phase Contrast and Rheinberg compared

Below are pictures from a recent article I posted on my web site showing different lighting techniques. Photos are of Wood by auto - fluorescence, Volvox by bright field, dark field, positive phase contrast, and Ribbon worm Rheinberg. Rheinberg filter set can be purchased on Ebay for about $30. Furt...
by RobBerdan
Sat Apr 13, 2019 11:17 pm
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4450

Tips on Buying a Microscope and suggestions for What to look at

I just posted an article on my web site including tips for buying a new and used microscope, also why some one might want to own a microscope and some subjects they can look at. https://www.canadiannaturephotographer.com/guide_buying_microscope.html Comments welcome Photos include, rotifer, onion ro...
by RobBerdan
Sat Apr 13, 2019 9:30 pm
Forum: Beginner's corner
Topic: Tips for Buying a Light Microscope
Replies: 2
Views: 3556

Tips for Buying a Light Microscope

Tips for Buying a Light Microscope - Compound, Inverted and Stereoscope and Why you might want to buy a Microscope I have just posted an article on my web site about Tips for buying a new or used microscope including stereoscopes, compound microscopes and inverted microscopes. I show some specimens...
by RobBerdan
Thu Mar 28, 2019 9:49 pm
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2119

Re: Crystal Mania

Hi Radazz - in answer to your question. Usually the crystals are bigger when they are allowed to form overnight of over a period of days. Slow growth seems to result in bigger crystals. I sometimes accelerate evaporation by heating the slides on a hot plate, but the crystals tend to be larger when d...
by RobBerdan
Thu Mar 28, 2019 9:39 pm
Forum: Pictures and Videos
Topic: Movie with Music showing Vitamin C crystals in Polarized Ligh
Replies: 3
Views: 1411

Movie with Music showing Vitamin C crystals in Polarized Ligh

I used Windows Movie Maker to create a short 3 minute slide show with music showing some of my favourite Crystals viewed with Polarizing microscopy. You can view the movie on Youtube here: https://www.youtube.com/watch?v=18DIc8BpRoA&feature=youtu.be If you have a fast connection you can view or down...
by RobBerdan
Tue Mar 26, 2019 5:44 pm
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2119

Re: Crystal Mania Questions

To answer your question how much Vitamin C to use - I generally make up a tube of 3 ml of deionized water, and 3 ml of 99% isopropyl alcohol - and add about small teaspoon of the powder and mix thouroughly - I use a laboratory vortex mixer but shaking would be fine to get as much of the powder disso...
by RobBerdan
Mon Mar 25, 2019 2:06 am
Forum: Pictures and Videos
Topic: Crystal Mania
Replies: 8
Views: 2119

Crystal Mania

I am working on photographing crystals with polarized light and trying to optimize conditions for growing Vitamin C crystals which in my opinion are some of the most beautiful crystals I have seen. A couple of things I have learned is that a mix of water and isopropyl alcohol seems to work best. Iso...
by RobBerdan
Mon Mar 25, 2019 1:47 am
Forum: Pictures and Videos
Topic: Rotifer from Hamburg Alster - Brachionus calyciflorus?
Replies: 5
Views: 1397

Re: Rotifer from Hamburg Alster

Very nice looks like you used Bright field and it worked very well.
RB
by RobBerdan
Tue Mar 19, 2019 10:42 pm
Forum: Pictures and Videos
Topic: Article about Crystals in Polarized light and by DIC
Replies: 9
Views: 2293

Re: Article about Crystals in Polarized light and by DIC

Thank you for your kind comments. I submitted an article to Micscape a few months ago and it can be found here: http://www.microscopy-uk.org.uk/mag/artoct18/rb-Photomicro-Science-Art.pdf - its about Science & Art. I am trying to promote microscopy in Canada and possibly start a microscopy club in Ca...
by RobBerdan
Mon Mar 18, 2019 12:49 am
Forum: Pictures and Videos
Topic: Article about Crystals in Polarized light and by DIC
Replies: 9
Views: 2293

Article about Crystals in Polarized light and by DIC

Dear microscopists - in an earlier post I mentioned I was working on an article for my web site about crystals in polarized light and by DIC. I have photographed, Vitamins, beer, alcohol, drugs and even human tears. I share my methods I used to produce the crystals which I hope will be of interest t...
by RobBerdan
Tue Mar 12, 2019 1:09 am
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1360

Re: Crystals and Polarized light

That is correct, however the sodium chloride induces crystals which can be seen and photographed easily with DIC. They are not birefringent, but somehow stimulate crystal formation where they would not otherwise form. Below these are Corona extra beer crystals formed in the presence of salt - otherw...
by RobBerdan
Mon Mar 11, 2019 6:52 pm
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1360

Re: Crystals and Polarized light

Thanks Bryan - I noticed you added table salt - it turns out that every alcoholic drink I add table salt do also crystallizes. So I tried NaOH no crystals. I tried NaCl (table salt) to, water, methanol, and ispropanol - no crystals suggesting it is the Cl ion that might be important or something in ...
by RobBerdan
Mon Mar 11, 2019 4:32 pm
Forum: Pictures and Videos
Topic: Crystals and Polarized light
Replies: 8
Views: 1360

Crystals and Polarized light

I have been experimenting with different drinks, beer and chemicals using polarized light and the technique produces amazing colors and patterns. The Microbe hunter owner provides a nice video on how to convert any microscope to a polarizing microscope for only a few dollars: https://www.youtube.com...