Search found 7 matches

by tocharian
Thu Apr 27, 2023 9:32 am
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 19
Views: 7936

Re: Epi-Fluorescence Microscopy and Deconvolution

I also found that Nikon NIS elements has the ability to do convolution- and even allows anyone to upload a file and they will produce the deconvoluted image for you that you can download on their web site - I just did a quick test yesterday and plant to test it further. https://deconv.laboratory-im...
by tocharian
Sat Dec 26, 2020 8:56 am
Forum: Specimens, samples and slides
Topic: Microbial biofilm sample pretreatment for SEM
Replies: 1
Views: 1404

Microbial biofilm sample pretreatment for SEM

Not sure if this is a relevant question for this forum, which seems to be exclusively for optical microscopy. But still hope someone can help on this. I need to use SEM to characterize the morphology of my bacterial biofilm. The standard protocol is to fix in glutaraldehyde (also in OsO4 according t...
by tocharian
Tue Jul 23, 2019 11:26 am
Forum: Specimens, samples and slides
Topic: Non-glass coverslips?
Replies: 1
Views: 2357

Non-glass coverslips?

My experiments requires the use of conductive coverslips. I recently used ITO coated polymer for this, and I wanted to visualize bacteria. However, under brightfield, using 40x dry objective and 100x oil objective I could not see actual shapes of bacteria, only blur bright spheres. The polymer, PET,...
by tocharian
Tue Apr 16, 2019 1:36 pm
Forum: Camera systems and imaging
Topic: For fluorescence microscopy, specialized microscope camera or DSLR?
Replies: 8
Views: 9269

For fluorescence microscopy, specialized microscope camera or DSLR?

I recently procured an entry-level fluorescence microscope, am now considering camera system for that. I’m using the microscope for scientific research. I’m not sure if it is worthy to buy specialized microscope cameras. Pre-owned older models of these cameras are more expensive than pre-owned full ...
by tocharian
Fri Nov 30, 2018 2:30 am
Forum: My microscope
Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
Replies: 5
Views: 5110

Re: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?

Although I am not familiar with the 41 model and its difference from the 53 model, I think I can input some relevant information. I understood from your recommendation that, the signal/noise ratio and resolution are more important than the absolute brightness. Also, am I right that, illumination an...
by tocharian
Thu Nov 29, 2018 4:08 pm
Forum: My microscope
Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
Replies: 5
Views: 5110

Re: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?

Hi, I would get quotes from other suppliers with different brands... Olympus is very good, but they are not the only one... BillT Thanks, I wanted to stay with mainstream brands, although my budget is limited. Olympus is the cheapest among the 4 brands, the other brands also have higher price in Ch...
by tocharian
Thu Nov 29, 2018 3:43 pm
Forum: My microscope
Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
Replies: 5
Views: 5110

Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?

I'm a researcher in China. I intended to procure an entry-level inverted fluorescence microscope for research purpose. I planned to use this to observe fluorescent bacteria cells (1-2 microns), and take pics with my DSLR . In the future, I want the system to generate 3-D view of microbial biofilm (t...