Search found 7 matches
- Thu Apr 27, 2023 9:32 am
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 7936
Re: Epi-Fluorescence Microscopy and Deconvolution
I also found that Nikon NIS elements has the ability to do convolution- and even allows anyone to upload a file and they will produce the deconvoluted image for you that you can download on their web site - I just did a quick test yesterday and plant to test it further. https://deconv.laboratory-im...
- Sat Dec 26, 2020 8:56 am
- Forum: Specimens, samples and slides
- Topic: Microbial biofilm sample pretreatment for SEM
- Replies: 1
- Views: 1404
Microbial biofilm sample pretreatment for SEM
Not sure if this is a relevant question for this forum, which seems to be exclusively for optical microscopy. But still hope someone can help on this. I need to use SEM to characterize the morphology of my bacterial biofilm. The standard protocol is to fix in glutaraldehyde (also in OsO4 according t...
- Tue Jul 23, 2019 11:26 am
- Forum: Specimens, samples and slides
- Topic: Non-glass coverslips?
- Replies: 1
- Views: 2357
Non-glass coverslips?
My experiments requires the use of conductive coverslips. I recently used ITO coated polymer for this, and I wanted to visualize bacteria. However, under brightfield, using 40x dry objective and 100x oil objective I could not see actual shapes of bacteria, only blur bright spheres. The polymer, PET,...
- Tue Apr 16, 2019 1:36 pm
- Forum: Camera systems and imaging
- Topic: For fluorescence microscopy, specialized microscope camera or DSLR?
- Replies: 8
- Views: 9269
For fluorescence microscopy, specialized microscope camera or DSLR?
I recently procured an entry-level fluorescence microscope, am now considering camera system for that. I’m using the microscope for scientific research. I’m not sure if it is worthy to buy specialized microscope cameras. Pre-owned older models of these cameras are more expensive than pre-owned full ...
- Fri Nov 30, 2018 2:30 am
- Forum: My microscope
- Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
- Replies: 5
- Views: 5110
Re: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
Although I am not familiar with the 41 model and its difference from the 53 model, I think I can input some relevant information. I understood from your recommendation that, the signal/noise ratio and resolution are more important than the absolute brightness. Also, am I right that, illumination an...
- Thu Nov 29, 2018 4:08 pm
- Forum: My microscope
- Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
- Replies: 5
- Views: 5110
Re: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
Hi, I would get quotes from other suppliers with different brands... Olympus is very good, but they are not the only one... BillT Thanks, I wanted to stay with mainstream brands, although my budget is limited. Olympus is the cheapest among the 4 brands, the other brands also have higher price in Ch...
- Thu Nov 29, 2018 3:43 pm
- Forum: My microscope
- Topic: Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
- Replies: 5
- Views: 5110
Olympus CKX41+trinocular observation tube split 50% light for camera, acceptable for taking fluorescence pics?
I'm a researcher in China. I intended to procure an entry-level inverted fluorescence microscope for research purpose. I planned to use this to observe fluorescent bacteria cells (1-2 microns), and take pics with my DSLR . In the future, I want the system to generate 3-D view of microbial biofilm (t...