Search found 100 matches

by lperepol
Fri Oct 18, 2019 5:20 pm
Forum: Beginner's corner
Topic: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?
Replies: 9
Views: 1111

Re: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?

Hi 75RR, I am saving up to upgrade to a DIC microscope. In the meantime, I was considering better objectives for the OMAX. And pass it on to my nephew. He is interested in biological sciences. Keeping expenses low. Not sure it would be worthwhile. I may be able to re-purpose higher-end objectives on...
by lperepol
Fri Oct 18, 2019 3:11 am
Forum: Beginner's corner
Topic: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?
Replies: 9
Views: 1111

Re: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?

Apochronaut, your detailed response is appreciated!! On your #3 is working distance. Comment. Would an oil objective with a 1.0 N.A. be better than a dry objective with a 0.95 N.A? I have two choices both are used and at the same price. 1) Nikon Plan APO 40x /0.95 DRY 160 TL with Collar RMS 2) Nikon...
by lperepol
Thu Oct 17, 2019 8:49 pm
Forum: Beginner's corner
Topic: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?
Replies: 9
Views: 1111

Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?

Can I expect a better picture (photograph) resolution from a Nikon Plan APO 40x objective screwed into an OMAX (Chinese Microscope) than the objectives that came with the OMAX microscope?
by lperepol
Wed Jul 31, 2019 11:15 am
Forum: Pictures and Videos
Topic: I thinl I am getting a bit better detail on nematodes.
Replies: 1
Views: 1185

I thinl I am getting a bit better detail on nematodes.

The 100x objectives Phase and Standard do not seem to resolves as well as the lower magnification objectives on the Omax. https://lh3.googleusercontent.com/0sNjqmC9-2CqFthaQFmhRzXDUglD0O7ln4H5c0f0a1s24HNVxg6BEh3wJ4RgsYBZF6PLwGPnn-ZpKuhvjITya-m-pLdkuDolAS7afFpAMnPcQhs0No0Lr0XHLJFapI9iT4LmfonIy8BtTwU8...
by lperepol
Tue Jul 30, 2019 1:18 pm
Forum: Beginner's corner
Topic: Correct application of oil for oil immersion?
Replies: 12
Views: 2664

Correct application of oil for oil immersion?

Oil immersion, does one apply oil on the bottom of the slide (top of condenser lens) and the bottom of the objective?
by lperepol
Sun Jul 28, 2019 2:29 pm
Forum: Beginner's corner
Topic: Glycerine vs glycerol and refractive index are both equivalent?
Replies: 13
Views: 2742

Re: Glycerine vs glycerol and refractive index are both equivalent?

Not sure if this helps MicroBob. A paper by Jonannes Hallman and Sergei A. Subbotin, Methods for Extraction, Processing and Detection of Plant and Soil Nematodes recommends that for permanent mounting of nematodes: "For permanent mounts, a very small drop of anhydrous glycerol (heated for 4h at 40°C...
by lperepol
Sun Jul 28, 2019 12:50 am
Forum: Beginner's corner
Topic: Coverslip spacers for permanent mounts?
Replies: 8
Views: 2727

Coverslip spacers for permanent mounts?

What if any spacers are recommended for providing a space between a coverslip and a slide for permanent mounts.
A strand from a fine braided electrical wire is one I can think of.
Are there commercial outlets? Stainless-steel wire, tungsten filaments of calibrated diameters?
by lperepol
Fri Jul 26, 2019 1:07 pm
Forum: Beginner's corner
Topic: Glycerine vs glycerol and refractive index are both equivalent?
Replies: 13
Views: 2742

Re: Glycerine vs glycerol and refractive index are both equivalent?

MicroBob >> do you know how to dry glycerine that has picked up moisture over time? The "A Simple Express Technique to Process Nematodes" assumes that water evaporates. Maybe store glycerine in a dry area for a few days -- like in a kitchen oven (turned off) or vegetable dehydrator? https://www.ncbi...
by lperepol
Thu Jul 25, 2019 7:58 pm
Forum: Beginner's corner
Topic: Glycerine vs glycerol and refractive index are both equivalent?
Replies: 13
Views: 2742

Glycerine vs glycerol and refractive index are both equivalent?

In respect to microscopy, refractive index and permanent mounts are both glycerine vs glycerol equivalent? Glycerine is available in pharmacies: PSP GO Glycerine - 100ml $4.49 https://www.londondrugs.com/psp-go-glycerine---100ml/L9487729.html glycerol is a bit more pricey Glycerol Wet Mounting Mediu...
by lperepol
Sat Jul 20, 2019 11:46 pm
Forum: Beginner's corner
Topic: Objectives?
Replies: 7
Views: 1490

Re: Objectives?

Hobbyst46 mentioned a link to Robert Otoole's comparison of objectives. 1) 4X OBJECTIVE LENS TEST: PART 1 and 2) $17 PLAN 4X OBJECTIVE The Big Surprise 1) https://www.closeuphotography.com/4x-lens-test/part-one-4x-for-less-than-100-dollars 2) https://www.closeuphotography.com/seventeen-dollar-plan-4...
by lperepol
Wed Jul 17, 2019 9:29 pm
Forum: Beginner's corner
Topic: Microscope Cameras from Microscop Manufactures VS Other
Replies: 28
Views: 2407

Re: Microscope Cameras from Microscop Manufactures VS Other

Not sure what I made up my mind about? Just researching and presenting the facts to the best of my ability. I will be buying a considerably better microscope than I currently have. I flip and flop a lot, and am leaning towards a Carl Zeiss microscope at the moment with a Canon camera. I have time to...
by lperepol
Wed Jul 17, 2019 2:08 pm
Forum: Beginner's corner
Topic: Microscope Cameras from Microscop Manufactures VS Other
Replies: 28
Views: 2407

Re: Microscope Cameras from Microscop Manufactures VS Other

I suspect that the software that comes with the cameras is escalating the price? Since there are few camera chip manufacturers. The Apple syndrome a $4,999.00 monitor that needs an optional stand for an additional $1,000.00. When generic Linux boxes are outperforming Macs in AI. Ridiculous! https://...
by lperepol
Wed Jul 17, 2019 8:06 am
Forum: Identification help
Topic: Fungus Identification Help
Replies: 12
Views: 4353

Re: Fungus Identification Help

Thanks apochronaut. I believe I may have a few varieties of Sclerotinia. I think it is common in the area -- also affecting squash and sunflowers. Locals seem to attribute most plant stem and roots issues with pythium. I am sure how that got started.
by lperepol
Wed Jul 17, 2019 2:04 am
Forum: Beginner's corner
Topic: Microscope Cameras from Microscop Manufactures VS Other
Replies: 28
Views: 2407

Re: Microscope Cameras from Microscop Manufactures VS Other

Which camera companies make their own sensors? "Canon states that they produce all their EOS sensors in-house but they have been on quite a large/old process (the biggest drawback of in-house production). They were rumoured to be opening a new fab at some point in 2014 and it now appears to be onlin...
by lperepol
Tue Jul 16, 2019 4:15 pm
Forum: Beginner's corner
Topic: Microscope Cameras from Microscop Manufactures VS Other
Replies: 28
Views: 2407

Microscope Cameras from Microscop Manufactures VS Other

Just wondering about pixel/price ratios. Leica DFC450 C Digital Camera & SW Kit 7,538.00 max. image size 2560x1920 pixel, 5 Mpixels $7,538.00 /5 --> $1507.6 per MP OMAX 18.0MP USB3.0 Digital Camera for Microscope $395.99 $395.99/18 --> $21.90 per MP Canon EOS R Mirrorless $1999.00 30. 3 Megapixel Fu...
by lperepol
Tue Jul 09, 2019 2:01 pm
Forum: Identification help
Topic: Nematode or not?
Replies: 4
Views: 735

Re: Nematode or not?

Thank you, Peter Freimann and apochronaut!!! "Bdelloids can only be identified by eye while they are alive because many of the characteristics significant to classification are related to feeding and crawling; however, genetic identification of bdelloids is possible on dead individuals. Once preserv...
by lperepol
Tue Jul 09, 2019 12:32 pm
Forum: Identification help
Topic: Nematode or not?
Replies: 4
Views: 735

Nematode or not?

Is this video of a nematode or something else? It does not move like a nematode that I have seen before, but it looks like it may have a stylet forming. I soaked a strawberry leave in water and a petri dish for 3 weeks and I am seeing these worms in the water now. The same strawberry leaves, three w...
by lperepol
Fri Jun 21, 2019 4:09 pm
Forum: Beginner's corner
Topic: Do it yourself double cover slips to enable observations from both sides?
Replies: 6
Views: 1411

Re: Do it yourself double cover slips to enable observations from both sides?

Come to think of it they may be easy to make. 1) Drill a 20 mm hole in the center of a strip of aluminum about the size of a slide (size 25 mm 75 mm), 2) Center and mount with nail polish, or beeswax a coverslip over the hole. 3) Add specimen, dry mount another coverslip, over the specimen, sandwich...
by lperepol
Fri Jun 21, 2019 7:37 am
Forum: Important announcements
Topic: New Youtube Channel
Replies: 10
Views: 5790

Thank you Oliver.

Thank you Oliver. Your videos are very informative!!!
by lperepol
Fri Jun 21, 2019 2:35 am
Forum: Beginner's corner
Topic: Do it yourself double cover slips to enable observations from both sides?
Replies: 6
Views: 1411

Do it yourself double cover slips to enable observations from both sides?

Anyone have techniques they can share for DIY (Do It Yourself) double cover slips to enable observations from both sides?

A DIY alternative to Cobb’s aluminium slide holders:
https://www.wur.nl/en/show/Cobbs-alumin ... ders-1.htm
by lperepol
Tue Jun 18, 2019 5:43 am
Forum: Beginner's corner
Topic: Objectives?
Replies: 7
Views: 1490

Objectives?

Will higher end objectives from Zeiss, Leica or Olympus thread into an Omax microscope?
And will they resolve images better than the objectives that come with an Omax or one can buy from Omax?
by lperepol
Wed May 29, 2019 10:39 pm
Forum: Beginner's corner
Topic: What techniques are being used to get such clear pictures?
Replies: 5
Views: 3596

Re: What techniques are being used to get such clear pictures?

I believe picture they are using differential interference contrast (DIC) for the picture.
by lperepol
Thu May 09, 2019 10:21 pm
Forum: Pictures and Videos
Topic: Three image comparisons Leica, Olympus and Omax
Replies: 6
Views: 1468

Re: Three image comparisons Leica, Olympus and Omax

Hobbyst46 I am not sure if the picture taken with the Omax was taken in phase contrast. Looks like brightfield. I may have removed the halos with some photo processing. I posted it because it was a similar species. The tooth is not that clear. Below is a picture using the Omax with phase contrast. T...
by lperepol
Thu May 09, 2019 5:21 pm
Forum: Pictures and Videos
Topic: Three image comparisons Leica, Olympus and Omax
Replies: 6
Views: 1468

Three image comparisons Leica, Olympus and Omax

Olympus BX51 with differential interference contrast (DIC) https://lh3.googleusercontent.com/dOu7oXMkrD9fGUR6T-FA0uTfnDMTVIjOfMDjMZ2fg1taweWVrhIW9WaleWh0gOBm25WZdG8ZOSnY0lNYotwkRlnsfMQqbEsmF9OKitTs-XLa4b1Ae8jY3Df_TtPJWqaNMxnl1KCz43oD_A7aKDI_hJ4r5zDz8xBXEYVVt4WgzOm1P6o1uqRxJr9-qs7ABsBElJLbOeC7Bk0e1IP...
by lperepol
Thu Apr 11, 2019 8:50 pm
Forum: Beginner's corner
Topic: Leica Vs Olympus Compound Microscopes what are your opinion?
Replies: 2
Views: 1961

Leica Vs Olympus Compound Microscopes what are your opinion?

I am looking to buy a higher end microscope and am considering Leica DM2000, Olympus BX43, Nikon Eclipse FN1.
I am dreaming for now and am scoping out prices. Does anyone have experience using these scopes?
by lperepol
Thu Mar 21, 2019 6:45 pm
Forum: Identification help
Topic: Fungus Identification Help
Replies: 12
Views: 4353

Re: Fungus Identification Help

Yes, I stained them with lactophenol cotton blue. >> Was that shot using PC or just BF? Bright Field I did find some something that looks like fungi that are a navy blue and some for sure fungi that is a bright red for some reason -- both of these were not stained. I see the red fungi on and in most...