MicrobeHunter.com Microscopy Forum

I'd carefully try to remove any remaining grease and wd40 residue with petroleum ether or lighter fluid before relubricating. wd40 is likely to kind of resinify after a short period. I am using synthetic watch libricants from moebius. For the rods i even switched from using grease to thin oil (Synt-...

Salut Michel,

very probably bought it from ebay or a similar platform several years ago for my M8 for around the same price.

C'est pas très loin, je vis juste à côté en suisse
Stefan

Hi Milou,

i've got that lense in very good condition going for 250€.
Where are you located?

Regards,
Stefan

Axiomat are M27, same as the newer Axio series. The first Axio series were only RMS i'd guess.
Rumor has it the final compensation correction on Axios is done by the tube lens.
So Axiomat probably won't fit at all to Axio.

Beside the square block format and the 100 lbs weight difference? Apparently Axiomats were the first infinity series from Zeiss. As i've got to know from the german microscopy forum, the objectives were not fully infinity corrected and depend on a correction lens above the turret to give full achrom...

one on ebay from a reputable seller but big $$$, item # 261361351446

Yes i am aware of that one, but the price is just ridiculous.

Hi,

I am desparately seeking for this objective in any cosmetic condition.

Regards,
Stefan

Dear Michael,

Ask / have a look here:
https://www.mikroskop-online.de

According to site search results, at least the 12/25 and the 25 papo objective prisms are available.
Found some condensor prisms too..

Regards,
Stefan

Hi Bob, the basic design has to be the same as with PZO, but my prism height is fixed. I didn't focus on details like specimen orientation or perfect rendering yet. And diatoms are not my favorite specimens for real DIC. But they look quite nice with slitDIC and i thought they are well suited for a ...

For comparison, Nomarski DIC

Second impression, slit approx 5.5mm:

Open: Slit: SlitDIC:

Hi, had to give it a try after reading the PlasDIC post. No idea about the origin of the Prism i've used, had it around for a while. Just some tape for the slit to begin. Tried on a Zeiss Jena Jenamed scope. IMG_7295.JPG First impressions, slit approx. 3.5 mm, 50x objective. Open aperture: P1140895....

Agree with Bob, coating probably worn or just dirty.
They should both look greenish when clean.
Petrolum ethers, as Bob suggested, might help remove some residues where soap or alcohol won't easily.

Hi, you are right regarding polarizer/analyzer position and PlasDIC. For PlasDIC you will usually find objective specific prisms and the polarizer and analyzer are both placed behind the sample. There once was an article in a german microscope magazine: https://www.zobodat.at/pdf/Mikrokosmos_72_0001...

Hi, bought a pair of those (last in the list: high eyepoint 10x/23 with rubber cups) in 2018: https://www.ebay.com/itm/1-Stuck-Stereomikroskop-Okular-WF10X-30mm-Weitfeld-Microscope-Eyepiece/263409538428?_trkparms=aid%3D1110006%26algo%3DHOMESPLICE.SIM%26ao%3D1%26asc%3D20200520130048%26meid%3D17dd9e27...

Without an IR/heat filter in the light path, my critters won't survive 60 seconds with some halogen 25 watt lamps.
Stefan

Outstanding capture, thx!

Edit:
Link removed. If anyone needs the document, PM please.

Cheers

For some fun, you could try to start with:
https://www.ebay.de/itm/Fluorescent-Dye ... xy1klRdope

Hi,
good news, hope you'll get it sorted with all the new filters.
Curious to see your results

yes 1.6x is the right mag for APS-C.

I know this shop as a good source for filters if you don't want to spend too much.

To start with excitation replacement:
https://www.ebay.de/itm/Ottico-Filtro-4 ... 23a9bd7ba6

Also your beamsplitter could be worn-down.

Stefan

Hi, for your type of sensor this might be the best option you can get. The T2 / 2.5 will crop to much of your frame unless you change your camera setup to full frame. Don't think there is any or much difference between your video option adapter and the one for digital compact cameras regarding size ...

Hi Tom, if you want to dive real deep into the subject, i can recommend you the following book: https://www.wiley-vch.de/de/fachgebiete/naturwissenschaften/molekularbiologie-der-zelle-978-3-527-34072-9 It could replace the bible on your bedside table for a while ;) An english downloadable copy here:...

Hi,

BHS or BHT?
My BHT tends to get quiet warm after a while, but i wouldn't call it hot.
Guess this is just usual heat dissipation from the lamp and the power transformer.

regards,
stefan

Hello Hunters, probably already introduced myself long time ago, maybe in the old Forum, but i can't find the entry anymore.. I'm mostly a quiet reader - don't talk to much in real life anyways - but i really love this Forum and microscopy. Thank you Oliver! Got my first scope around 8 years ago at ...

Thank you so much for running and maintaining microbehunter and this great forum.

Not that easy to answer, it would help to let us know which condenser you have. You'll have to build some kind of support to fit under/inside the condenser where you could put some stops. But you won't be able to realize good darkfield for higher magnifications that way anyways. Maybe this could giv...

Hi Bob, although today i mostly enjoy the pure simplicity of use and the nice (heavy understatement) optics of my Jenamed, using my Phomi 3 has always been a special affair. :geek: Honestly you should try one out by yourself first to get an impression, but i always loved the touch and feel of it. I ...

Hello,

your last picture shows a diopter adjustable, FOV25 and 30mm tube diameter eyepiece from the cf-250 era of Zeiss Jena.
Fits to Jenamed, Jenaval, Jenalumar and many more.
Hope this helps.

Regards,
Stefan