Search found 57 matches

by DrPhoxinus
Fri Jul 03, 2020 6:06 pm
Forum: Introduce yourself
Topic: Introduction
Replies: 6
Views: 403

Re: Introduction

So where are you with the scopes?

What do you think of the 150?

I had a Reichert 150 and it was university surplus.
It only had achromatic objectives.

I bought an older AO 150 and with plan achros it is easy for a kid to use and take pictures.

Gerard
by DrPhoxinus
Fri Jul 03, 2020 5:58 pm
Forum: Introduce yourself
Topic: Hello!
Replies: 1
Views: 324

Re: Hello!

Welcome to the forum.

If you haven’t already done so, get yourself some concave slides and google hay infusion.

You will get a great group of microbes in under two weeks.

My favorite objective power is 20x.

I wish more scopes came with them

Gerard
by DrPhoxinus
Fri Jul 03, 2020 5:44 pm
Forum: Pictures and Videos
Topic: Cyanobacteria in Freshwater ponds
Replies: 5
Views: 398

Re: Cyanobacteria in Freshwater ponds

Acridine Orange is a powerful mutagen that causes frameshift mutations in DNA. Great image

Gerard
by DrPhoxinus
Fri Jul 03, 2020 5:42 pm
Forum: Introduce yourself
Topic: I am John
Replies: 1
Views: 49

Re: I am John

I am good John.

Do you have or want a microscope?

Gerard
by DrPhoxinus
Mon Jun 29, 2020 2:24 pm
Forum: Microscopes and optics
Topic: What to do about coating damage in Ortholux trinoc head
Replies: 4
Views: 171

Re: What to do about coating damage in Ortholux trinoc head

Thanks Apochronaut, It isn't dirt and reflection of the surface looks like a very uneven and damaged coating. Perhaps in the past someone was zealous with an alcohol swab. It may be that the low light level with the 20x exacerbates the problem compared to the 10x I am cheered that ethanol has the po...
by DrPhoxinus
Mon Jun 29, 2020 10:17 am
Forum: Pictures and Videos
Topic: I see stars - Asterionella diatoms in dark field
Replies: 7
Views: 311

Re: I see stars - Asterionella diatoms in dark field

Does the scope use 6v or 12?

Do you step down 12v to 6?

What USB converter?

Thanks

Gerard
by DrPhoxinus
Sat Jun 27, 2020 4:11 pm
Forum: Microscopes and optics
Topic: What to do about coating damage in Ortholux trinoc head
Replies: 4
Views: 171

What to do about coating damage in Ortholux trinoc head

Now that I have a LED flashlight to illumine an Ortholux I, I have found a problem with the trinocular head. With a 10X objective the image looks fine, but with a 20X the background of the image looks like a dusty debris field. Moving the slider moves the "dust"/ Upon looking into the head, the roun...
by DrPhoxinus
Fri Jun 26, 2020 1:55 pm
Forum: Introduce yourself
Topic: new to microscopy need help properly identifying my microscope
Replies: 13
Views: 340

Re: new to microscopy need help properly identifying my microscope

I am beginning to wonder whether there is a microscope model that Charles doesn't own.

Charles, can you give us a count and a model breakdown?

Gerard
by DrPhoxinus
Fri Jun 26, 2020 1:53 pm
Forum: Microscopy accessories
Topic: A LED flashlight worth consideration
Replies: 3
Views: 199

Re: A LED flashlight worth consideration

You are welcome Peter, Apparently I bought the first one, and the vendor has sold a dozen since my post. I have two Ortholux I scopes without their lamphouses, one doesn't even have a collector lens. So this LED flashlight seemed great for those microscopes. I am always looking for small gizmos. Som...
by DrPhoxinus
Wed Jun 24, 2020 9:04 pm
Forum: Introduce yourself
Topic: new to microscopy need help properly identifying my microscope
Replies: 13
Views: 340

Re: new to microscopy need help properly identifying my microscope

Ken, Welcome to the forum. I am about 5 to 6 diopters out and haven’t had a problem. However when a normal vision family wants a look then refocusing is in order. Among my scopes I have an AO 150 two series 10 and a 410 which looks a little like yours in style. Maybe compressed air can blow the dust...
by DrPhoxinus
Wed Jun 24, 2020 6:01 pm
Forum: Microscopy accessories
Topic: A LED flashlight worth consideration
Replies: 3
Views: 199

A LED flashlight worth consideration

The features of most LED flashlights complicate their use with microscopes. That on/off switch, the battery, and a design that usually doesn't include a long on time. For microscopy less is more Well I found one that doesn't have those. When I saw this on ebay it looked liked a focusable led too sho...
by DrPhoxinus
Sun Jun 21, 2020 2:58 pm
Forum: Introduce yourself
Topic: Introduction
Replies: 6
Views: 403

Re: Introduction

Hans When we decided to home school our daughter I went a limitless crazy on microscopes. I have 11. I have an AO 150 that I upgraded to plan achro objectives and it is a great scope for quick observations. I have 2 series 10, one for brightfield and one for phase. The scopes were well used and the ...
by DrPhoxinus
Sat Jun 20, 2020 1:37 pm
Forum: Introduce yourself
Topic: Introduction
Replies: 6
Views: 403

Re: Introduction

Welcome Hans,

I was given a Microstar IV with a Series 10 trinocular head so that is an option. I find the blue filter doesn’t always function as smoothly as I would like.

Best Wishes,

Gerard
by DrPhoxinus
Fri Jun 12, 2020 8:15 pm
Forum: Illumination Techniques
Topic: Could i get a cooler bulb?
Replies: 34
Views: 924

Re: Could i get a cooler bulb?

Halogens operate at 260 C
any lower and they darken and fail prematurely
by DrPhoxinus
Tue Jun 09, 2020 3:45 pm
Forum: Introduce yourself
Topic: Bio Major (advice on future career and academic goals)
Replies: 28
Views: 700

Re: Bio Major (advice on future career and academic goals)

I have to agree with the wisdom of Leitzcycler. As a postdoc I wrote some NSF grants that scored higher than my advisor's, but I didn't have a tenure track job so I didn't get the money. I took a job in a University computer center for double the money and only a 40 hour week. I did my research on t...
by DrPhoxinus
Mon Jun 08, 2020 8:37 pm
Forum: Introduce yourself
Topic: Bio Major (advice on future career and academic goals)
Replies: 28
Views: 700

Re: Bio Major (advice on future career and academic goals)

I concur with wporter. I got involved with computing after doing a ANOVA on a calculator, and vowed never to do that again. Sir Ronald Fisher, a genius biologist, invented ANOVA to test fertilizers on corn. Much of statistics has it origin in biology. The grease in my Zeiss I used in grad school has...
by DrPhoxinus
Mon Jun 08, 2020 4:46 pm
Forum: Introduce yourself
Topic: Bio Major (advice on future career and academic goals)
Replies: 28
Views: 700

Re: Bio Major (advice on future career and academic goals)

I am a biologist in evolutionary biology with a minor in computer science. I think it is important for a bio student to have a trade. My computer skills is a bigger share of my income than my bio teaching. Between ecology and microbiology I would choose micro. You will have skills useful in a lab an...
by DrPhoxinus
Sun Jun 07, 2020 9:38 pm
Forum: Microscopy accessories
Topic: Power supplies, bulbs and Ohm's law
Replies: 0
Views: 234

Power supplies, bulbs and Ohm's law

I thought I would relate some of my experiences powering lights in microscopes. I have 11 scopes crammed on a table because of the proverbial wife's unemployed relative needing a room. This means I have to use the scopes in a transient manner somewhere else in the house. Not all are self contained f...
by DrPhoxinus
Sun Jun 07, 2020 4:03 pm
Forum: Microscopes and optics
Topic: AO Spencer Series 4 Trinocular Problem
Replies: 5
Views: 262

Re: AO Spencer Series 4 Trinocular Problem

This looks like a good GE 1493 given the picture of the filament

https://www.ebay.com/itm/GE-1493-Swift- ... SwxvtcpNsH

Gerard
by DrPhoxinus
Sun Jun 07, 2020 3:52 pm
Forum: Beginner's corner
Topic: Starter wet slide kit?
Replies: 21
Views: 605

Re: Starter wet slide kit?

I have had good luck getting them from staples.com of all places

https://www.staples.com/Kimtech-Science ... uct_808376

Gerard
by DrPhoxinus
Fri Jun 05, 2020 11:38 am
Forum: Illumination Techniques
Topic: Flashlight as permanent illuminator inside the base of the microscope ?
Replies: 24
Views: 1221

Re: Flashlight as permanent illuminator inside the base of the microscope ?

I see that there are multiple leds in the lamp.
Have you found candidates for diffusers?
Something frosted? I take it the lamp does not have a focus.

Gerard
by DrPhoxinus
Sun May 24, 2020 11:54 pm
Forum: Identification help
Topic: I tried to look at red blood cells. Are these Echinocytes?
Replies: 1
Views: 164

Re: I tried to look at red blood cells. Are these Echinocytes?

Looks like crenation of RBCs due to hypertonic conditions

Gerard
by DrPhoxinus
Wed May 13, 2020 6:34 pm
Forum: Pictures and Videos
Topic: Fragilaria
Replies: 13
Views: 555

Re: Fragilaria

Thanks for the information Wes, Years ago I worked on the karyotype of the European Mud Minnow, Umbra krameri, endemic to the Danube and Dniester river drainages. I think it is threatened now. It has 44 chromosomes and the North American Umbra limi has 22 metacentrics, probably Robertsonian fusions....
by DrPhoxinus
Wed May 13, 2020 5:26 pm
Forum: Pictures and Videos
Topic: Fragilaria
Replies: 13
Views: 555

Re: Fragilaria

Great photos.

Where is the stream and what do you think was the water temp?

Gerard
by DrPhoxinus
Tue May 12, 2020 12:18 pm
Forum: Camera systems and imaging
Topic: AO Triocular vs Beam Splitter
Replies: 46
Views: 1661

Re: AO Triocular vs Beam Splitter

I searched eBay for American optical trinocular and the first. hit was a 1043 head for 60 or best offer and then a 110 scope for a 100

I think I have an extra aluminum tube for the trinoc somewhere

Gerard
by DrPhoxinus
Tue May 12, 2020 12:04 pm
Forum: Microscopes and optics
Topic: what is the size of the annuli in the Leitz 402a condenser
Replies: 17
Views: 2096

Re: what is the size of the annuli in the Leitz 402a condenser

It’s a Zeiss Standard 16.

Looking through the Zeiss Standard Manual a Model 14 has a 4 objective nosepiece and the Model 16 has a 5 objective one.

Ah, Zeiss taxonomy
by DrPhoxinus
Sat May 09, 2020 11:18 pm
Forum: Illumination Techniques
Topic: Flashlight as permanent illuminator inside the base of the microscope ?
Replies: 24
Views: 1221

Re: Flashlight as permanent illuminator inside the base of the microscope ?

Pete,

Thanks. Could you post a links from eBay for likely candidates


Gerard
by DrPhoxinus
Sat May 09, 2020 2:28 pm
Forum: Illumination Techniques
Topic: Flashlight as permanent illuminator inside the base of the microscope ?
Replies: 24
Views: 1221

Re: Flashlight as permanent illuminator inside the base of the microscope ?

I appreciate your post. Just this morning I was on eBay and put a number of focusable usb flashlights on my watchlist. Some are down to under $10 and have the usb connector at the end but don’t come with the 3.7 V battery. The heat thing could be serious as they probably weren’t designed to be on ve...
by DrPhoxinus
Sat May 09, 2020 2:16 pm
Forum: Microscopes and optics
Topic: Modern 10X Eyepieces for 1947 Leitz?
Replies: 3
Views: 537

Re: Modern 10X Eyepieces for 1947 Leitz?

Maybe my eyes aren’t critical enough and I don’t go above the 40x objective, but I have been disappointed by the field of view in the old Leitz eyepieces. Instead I plopped in American Optical 10x Cat# 176 and 15x Cat # 184 and I have been happy with the results. Has anyone else done this and had is...
by DrPhoxinus
Tue May 05, 2020 6:11 pm
Forum: Beginner's corner
Topic: 60x 0.17 or 63x 0 objective
Replies: 8
Views: 629

Re: 60x 0.17 or 63x 0 objective

I bought a 60 x and regretted it. You have to ask yourself what do you want it for. In terms of resolution the rule of thumb is don't exceed 500 times the numerical aperture, otherwise it is empty resolution 500 *.85 =425 so stuff is not so good with a 10x eyepiece for 600x. So many people are preoc...