Search found 94 matches
- Tue Apr 23, 2024 8:40 pm
- Forum: Pictures and Videos
- Topic: Euglenids, possibly Family Peranemidae in darkfield & DIC
- Replies: 0
- Views: 269
Euglenids, possibly Family Peranemidae in darkfield & DIC
Played around with my darkfield condenser and 50X oil objective and got the following image. The darkfield video doesn't add much, just more of the same. The second video shows similar euglenids, this time in DIC at 40X and 100X. https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEisqqMn-RseC...
- Sat Apr 20, 2024 2:13 am
- Forum: Identification help
- Topic: Another mystery ciliate
- Replies: 2
- Views: 176
Re: Another mystery ciliate
Thanks, that makes sense.
- Fri Apr 19, 2024 9:29 pm
- Forum: Identification help
- Topic: Another mystery ciliate
- Replies: 2
- Views: 176
Another mystery ciliate
I captured this little guy zooming around my slide and finally decided to take an image and measure it. Turns out he comes in as a super lightweight at 5.6 um. None of my searches show any ciliate on the planet being smaller than 10 um. I agree the image is practically useless but is the best I've b...
- Fri Apr 19, 2024 4:20 pm
- Forum: Identification help
- Topic: Any idea what this is?
- Replies: 2
- Views: 266
Re: Any idea what this is?
I watched this specimen as long as my patience allowed and saw no movement other than the hint of moving cilia. I suppose a large squashed rotifer could look like that.
Or perhaps it’s just another unidentified microscopic object. I seem to have no trouble finding those.
Harry
Or perhaps it’s just another unidentified microscopic object. I seem to have no trouble finding those.
Harry
- Thu Apr 18, 2024 5:35 pm
- Forum: Identification help
- Topic: Any idea what this is?
- Replies: 2
- Views: 266
Any idea what this is?
I assume this is a ciliate of some sort but am at odds with getting any closer than that.
https://www.youtube.com/watch?v=O20RWamwnes
https://www.youtube.com/watch?v=O20RWamwnes
- Thu Apr 11, 2024 10:12 am
- Forum: Miscellaneous
- Topic: Interesting article on worm vision
- Replies: 2
- Views: 206
- Mon Apr 08, 2024 9:51 pm
- Forum: Pictures and Videos
- Topic: pollen
- Replies: 1
- Views: 1482
pollen
Oliver posted a live stream this weekend on finding pollen in honey. It seemed interesting and I duplicated what he did. Here are a few photos. https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg-hFJinJhjU5cYpxRjKRBp4TId5OaKfGM5zWMW3GlQkW8k_0WlpDAwMVFIlolKoAkjOfGpr8A48NtTBrnIKSTn-Y608zgvQTK...
- Mon Apr 08, 2024 4:10 pm
- Forum: Pictures and Videos
- Topic: What Does It Mean To Say One Ciliate Eats Another
- Replies: 21
- Views: 2281
Re: What Does It Mean To Say One Ciliate Eats Another
Thanks. Thoughtful thread, I learned a lot. Keep those questions coming, you make interesting queries.
Harry
Harry
- Mon Apr 08, 2024 1:45 pm
- Forum: Pictures and Videos
- Topic: What Does It Mean To Say One Ciliate Eats Another
- Replies: 21
- Views: 2281
Re: What Does It Mean To Say One Ciliate Eats Another
Hey Don, although I live south of you I suspect we share a number of season related problems. :) I’ve had a fairly successful winter of viewing by increasing the number of specimen jars, feeding them different foods and adding different plant species. Although species numbers settled down I was usua...
- Fri Apr 05, 2024 11:45 am
- Forum: Pictures and Videos
- Topic: Condylostoma ciliate
- Replies: 4
- Views: 1276
Re: Condylostoma ciliate
Very nice video, the detail is outstanding. Far better than anything I’ve been able to do.
Harry
Harry
- Wed Apr 03, 2024 9:22 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
Quick question regarding diatoms to test resolution, are there species where the spacings are consistent enough across individuals that people are using them for resolution test without measuring? Or are people always measuring the spacing on their individual specimen before doing resolution tests?...
- Wed Apr 03, 2024 5:13 pm
- Forum: Pictures and Videos
- Topic: Marine Foraminifera on the move
- Replies: 10
- Views: 955
Re: Marine Foraminifera on the move
Wonderful! Those reticulopodia in the early frames look really long. Do they extend them, latch onto something and then pull themselves along?
Harry
Harry
- Wed Apr 03, 2024 5:07 pm
- Forum: Identification help
- Topic: Dwarf forms of Stentor: How do they originate?
- Replies: 3
- Views: 663
Re: Dwarf forms of Stentor: How do they originate?
Nice imaging! That little guy/gal is acting like a runway model, showing off his/her best sides. Sorry, no idea how they form.
Harry
Harry
- Tue Apr 02, 2024 6:55 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
Old and tired....me too! I spent a couple of days last week reading up on MTF and decided I'd rather be focusing my microscope on a water droplet. If anyone wants more info than he/she needs on MTF a book that covers the subject in terms of microscopy is "Better Microscopy, Vol III" by D.J. Jackson....
- Tue Apr 02, 2024 3:42 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
It would be useful to hear whether the following numbers sound reasonable based on practical experience of yourself or anyone else who wants to comment: 1.4 NA objective with 0.9 NA illumination gives 13% worse resolution. 1.4 NA objective with 0.1 NA illumination (about as small as most condenser ...
- Tue Apr 02, 2024 2:07 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
Thanks zzffnn, I’ll order some and give it a try.
The oil I have is Harleco Immersion Oil and it’s designated as “High Viscosity - 1250 centistokes so that may be the issue.
Harry
The oil I have is Harleco Immersion Oil and it’s designated as “High Viscosity - 1250 centistokes so that may be the issue.
Harry
- Tue Apr 02, 2024 1:56 pm
- Forum: Pictures and Videos
- Topic: Everything looks better in slow motion
- Replies: 12
- Views: 1796
Re: Everything looks better in slow motion
Thanks for the comments, bkt. I’ve got a flash adapter on the way to me that should help with many of my challenges.
Harry
Harry
- Mon Apr 01, 2024 6:13 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
I have a couple of Zeiss apl universal condensers, a .63 NA version and a 1.4 NA version. Unfortunately my high NA objectives are phase objectives, further muddying the waters. I tried a quick test yesterday and the results weren't noteworthy. I also have Kemp's 8 form test plate should I be able to...
- Mon Apr 01, 2024 3:23 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
I’m not sure what your point is since I agree with both. Nor do I want to get into arguments about the theory or mathematics behind microscopy optics, you clearly know more about both than I do. I’m merely exploring whether there is a practical way to determine the effect of using a low NA dry conde...
- Mon Apr 01, 2024 12:46 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5254
Re: Averaging condenser and objective numerical aperture when predicting resolution
Despite my failure to do so after a quick look it seems to me a clever group could design an experiment to solve or at least better understand our dilemma. I think the math for condenser NA and separately, objective NA is well estabished and understood. Where all the ambiguity comes in is when the t...
- Sun Mar 31, 2024 5:58 pm
- Forum: Identification help
- Topic: rotifer ID
- Replies: 0
- Views: 943
rotifer ID
Anyone have any idea what type of rotifer this is? I've gone through a couple of keys but I appear to be hopeless at this.
https://www.youtube.com/watch?v=reqZGqVobrg
https://www.youtube.com/watch?v=reqZGqVobrg
- Sun Mar 31, 2024 3:12 pm
- Forum: Pictures and Videos
- Topic: Everything looks better in slow motion
- Replies: 12
- Views: 1796
Re: Everything looks better in slow motion
On the subject of cilia, I was lucky enough to capture these cilia, top left of the ciliate, in their "return" phase. https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEihcSpLdgUofb5woaytyPpRsFE-qNG8nOBYDoGUdJvisz7U0niqR11Dmyx8CkaEY9h_j-f8sEa6TrPknfuEnmlakoLBVmxt2AL9kCYn7SHKaFZRNjnfE_L65-pcX...
- Sun Mar 31, 2024 2:59 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
You're more than welcome. In my search for good photomicrography techniques I came across another webste: https://www.plingfactory.de/pling.html
I believe he and Kreutz collaborate on some things.
Harry
I believe he and Kreutz collaborate on some things.
Harry
- Sat Mar 30, 2024 6:54 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
Thanks to everyone who contributed, it was a fun ride. Scarodactyl, you'll be pleased to know that I'm now following a few items on eBay, including a plan apo 20X.
I think I still want a 60X but it will be a dry, either plan apo or plan fluor, whichever falls off the truck first.
Harry
I think I still want a 60X but it will be a dry, either plan apo or plan fluor, whichever falls off the truck first.
Harry
- Fri Mar 29, 2024 5:05 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
Sounds like we should be able to test this empirically by checking out the circle of light hitting the objective back with a phase telescope. Anybody have any idea how NA might relate to diameter or area of illumination?
Never mind, tried a couple of things but nothing definitive. Back to having fun.
Never mind, tried a couple of things but nothing definitive. Back to having fun.
- Fri Mar 29, 2024 10:05 am
- Forum: Pictures and Videos
- Topic: Everything looks better in slow motion
- Replies: 12
- Views: 1796
Re: Everything looks better in slow motion
Very nice, enjoy these little clips. No idea what that is beyond saying it’s some type of peritrich. Perhaps someone more knowledgeable can offer more.
Keep them coming.
Harry
Keep them coming.
Harry
- Thu Mar 28, 2024 9:38 pm
- Forum: Pictures and Videos
- Topic: Stylonychia cyst
- Replies: 0
- Views: 1249
Stylonychia cyst
One of my jars has nothing but what I believe are ciliates from the Genus Stylonychia. Looking at a water drop from that jar also revealed a large number of what look like cysts, hence likely Stylonychia cysts. I stacked 7 images to get this one. https://blogger.googleusercontent.com/img/b/R29vZ2xl/...
- Thu Mar 28, 2024 6:33 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
I have a strong feeling that with a dry condenser one is never going to see the potential available in those 1.4 NA plan apo objectives. I’m going to engage in some day dreaming now. I’ve got a Zeiss apl 1.40 condenser sitting around doing nothing. What’s to stop me from slipping out of the lab and ...
- Wed Mar 27, 2024 8:35 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
The money might be better spent on something like a mirrorless camera that can capture every bit of resolution you already have, while eliminating vibration and the like. Pete, I've gone back to this comment, realizing I haven't a clue about how mirrorless cameras compare with the old Canon Live Vi...
- Wed Mar 27, 2024 2:03 am
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7706
Re: Do I need another objective?
I'd watch for a favorable auction, not a buy it now. I got mine for 450, this one is starting at 600 but it's also VC (a little better than apo) https://www.ebay.com/itm/315246893929 Thanks, great tip! Unfortunately many sellers, including this one, won’t ship to Canada. I’ll start watching the auc...