Search found 270 matches

by Wes
Tue Oct 22, 2019 3:12 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 136

Re: Marine Alga

Quality shot! Any idea what the pink stuff inside is?
by Wes
Sun Oct 20, 2019 12:18 pm
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 121

Re: Disposing of stains from home microscopy lab

Oxidizing can produce even worse chemicals. For instance, bleach and ethidium bromide makes much worse chemicals than ethidium bromide. California actually allows 9 ng/L of ethidium bromide in the normal sewer system in some cities. I doubt you produce enough to cause any harm however you dispose o...
by Wes
Sat Oct 19, 2019 4:43 pm
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 121

Re: Disposing of stains from home microscopy lab

I would filter the stain solutions through activated charcoal. It worked for coomassie brilliant blue so it's very likely to work for you too. You can also add a little bleach or potassium permanganate to oxidize the dyes.
by Wes
Sat Oct 19, 2019 4:28 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 114

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Many thanks Tom, Michael and John! Can you give an idea of the scale of the images, or at least the objective powers used? I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calcula...
by Wes
Fri Oct 18, 2019 10:47 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 114

Determining the molecular organisation of biological materials using polarized light microscopy

One can use a polarized light microscope together with a first order (full wave) retardation plate to determine, among many other things, the molecular organization of polymeric organic materials. The specimen Here I decided to apply this idea to isolated xylem fibers (or tracheids, perhaps mrsonchu...
by Wes
Thu Oct 17, 2019 9:03 pm
Forum: Beginner's corner
Topic: Nikon Plan APO 40x 160 TL in an OMAX (Chinese Microscope) 160 TL?
Replies: 7
Views: 143

Re: Nikon Plan APO 40x in an OMAX (Chinese Microscope)?

Are they designed for the same tube length? If yes your best chances are using a matched Nikon eyepiece.
by Wes
Thu Oct 17, 2019 7:40 pm
Forum: Pictures and Videos
Topic: Podocystis sp.
Replies: 4
Views: 53

Re: Podocystis sp.

I agree with the members above me that this is a spectacular image.
by Wes
Wed Oct 16, 2019 10:15 pm
Forum: Identification help
Topic: Amoeboid infected with parasites?
Replies: 7
Views: 577

Re: Amoeboid infected with parasites?

ID aside, this is a very interesting and attractive image. From your yeast fed culture ? Yes, its from the yeast fed culture which I am now feeding with corn and amoeba are plentiful. This particular object I saw only once, usually there are many specimens of each species I find but this object was...
by Wes
Wed Oct 16, 2019 5:46 pm
Forum: Pictures and Videos
Topic: Mastigamoeba
Replies: 3
Views: 110

Re: Mastigamoeba

Great specimen, kinda makes me want to go out and collect some pond algae myself :lol: Excellent video documentation well!

Are you making a culture out of this sample?
by Wes
Wed Oct 16, 2019 2:12 pm
Forum: Pictures and Videos
Topic: Licmophora colosalis
Replies: 4
Views: 128

Re: Licmophora colosalis

Beautiful image 75'!

Do you first make the stack and then stitch?
by Wes
Tue Oct 15, 2019 6:38 am
Forum: Pictures and Videos
Topic: Stenostomum eats the ciliates
Replies: 7
Views: 161

Re: Stenostomum eats the ciliates

That was really cool to watch, thanks for posting!
by Wes
Mon Oct 14, 2019 7:08 pm
Forum: Introduce yourself
Topic: Grüetzi
Replies: 4
Views: 90

Re: Grüetzi

Welcome!

Do post some images of your microscope and related stuff! Sounds interesting :)
by Wes
Mon Oct 14, 2019 8:04 am
Forum: Illumination Techniques
Topic: Diverse views of Pleurosigma on a strew slide
Replies: 8
Views: 318

Re: Diverse views of Pleurosigma on a strew slide

Thank you for posting, the single phase shot is by far the best in my opinion. To deal with vibrations I usually set a shutter release timer of a few seconds to provide enough time for residual vibrations to subside. Alternatively I connect the camera to my laptop, when I'm ready to take the shot I ...
by Wes
Mon Oct 14, 2019 7:58 am
Forum: Pictures and Videos
Topic: Salt, focus stacking
Replies: 8
Views: 218

Re: Salt, focus stacking

Oчень хорошо!

Welcome and a great stack of such an inconspicuous every day item.
by Wes
Sat Oct 12, 2019 8:32 pm
Forum: My microscope
Topic: DIY DIC Effect on Darkfield.... unreal
Replies: 6
Views: 590

Re: DIY DIC Effect on Darkfield.... unreal

They do look similar but oblique only works well with low to medium NA objectives.
by Wes
Sat Oct 12, 2019 8:30 pm
Forum: Pictures and Videos
Topic: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots
Replies: 10
Views: 537

Re: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots

...Its a nice microscope with multiple lasers and top of the line planapo objectives. Maybe one day decades from now they will start popping up on ebay and come into the possession of the highest bidder. Those multiple lasers, among other features, make present day confocal an unlikely candidate fo...
by Wes
Sat Oct 12, 2019 5:06 pm
Forum: Identification help
Topic: Amoeboid infected with parasites?
Replies: 7
Views: 577

Re: Amoeboid infected with parasites?

I regret not taking a phase contrast image, that might have been nice.
by Wes
Sat Oct 12, 2019 5:04 pm
Forum: Pictures and Videos
Topic: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots
Replies: 10
Views: 537

Re: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots

Again a great rotating image, Wes! To make the shape of things understandable these animations are very interesting. Would your new toy be useful to genetare some stunning mitosis pictures? ;) Thanks Bob, I believe it would be the perfect instrument to catch high resolution mitotic scenery. Below i...
by Wes
Sat Oct 12, 2019 3:53 pm
Forum: Pictures and Videos
Topic: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots
Replies: 10
Views: 537

Re: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots

What a great experience, I'd be interested to hear more about it ! Thanks mintakax, I had to learn to use the confocal microscope in order to capture and quantify single RNA molecules from in situ hybridization and as far as the microscopy part goes I used readily available fluorescent seedlings as...
by Wes
Sat Oct 12, 2019 3:33 pm
Forum: Pictures and Videos
Topic: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots
Replies: 10
Views: 537

Re: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots

Hobbyst46 wrote:
Sat Oct 12, 2019 3:29 pm
There are specific mitochondrial stains, yet they are very expensive....
Yes, there was mitotracker in the lab but we had the GFP seedlings so it was one step less in the preparation
by Wes
Sat Oct 12, 2019 2:51 pm
Forum: Pictures and Videos
Topic: Fluorescent doughnut-shaped mitochondria from Arabidopsis roots
Replies: 10
Views: 537

Fluorescent doughnut-shaped mitochondria from Arabidopsis roots

I got the opportunity to play with a Zeiss LSM880 confocal laser scanning microscope. For this purpose I had to prepare a specimen that was the root of Arabidopsis thaliana seedling. This particular strain of Arabidopsis expresses a green fluorescent protein which contains a mitochondrial targeting ...
by Wes
Sat Oct 12, 2019 8:30 am
Forum: Illumination Techniques
Topic: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.
Replies: 15
Views: 871

Re: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.

In case anyone tries relief PC please share your results here. I tried something but so far it just looked rather bad.
by Wes
Sat Oct 12, 2019 8:27 am
Forum: Identification help
Topic: Amoeboid infected with parasites?
Replies: 7
Views: 577

Re: Amoeboid infected with parasites?

I see three possibilities: food, gametes, parasites. The 5 objects look nothing like the food vacuoles in any of the protists I've seen in this sample, usually they have a thin membrane and are filled with either yeast, algae or bacteria. The structures here are a bit more intricate, there is an out...
by Wes
Fri Oct 11, 2019 2:35 pm
Forum: Pictures and Videos
Topic: Amoeba yeast feast
Replies: 15
Views: 858

Re: Amoeba yeast feast

Looks like plain old Oblique using Phase objectives to me. You are basically right, its a combination of the two. Speaking of illumination techniques I find relief phase contrast interesting http://zeiss-campus.magnet.fsu.edu/referencelibrary/pdfs/Piper_Micro_and_Analysis_21-9-2007.pdf Haven't yet ...
by Wes
Fri Oct 11, 2019 8:30 am
Forum: Microscopes and optics
Topic: Opening Zeiss 47 30 28 Trinocular Head
Replies: 11
Views: 633

Re: Opening Zeiss 47 30 28 Trinocular Head

In this case though I was on an inspection. Seemed quite clean actually, will have to look elsewhere for the culprits. Finding that one speck of dirt which always get on your photos can be a very challenging task. Have you ruled out the eyepiece, relay lens and sensor (you can get an idea by indivi...
by Wes
Fri Oct 11, 2019 8:26 am
Forum: Pictures and Videos
Topic: Ciliate feeding frenzy
Replies: 5
Views: 398

Re: Ciliate feeding frenzy

I'd be most delighted to get exploding amoeba populations in my cultures (maybe not the brain eating ones but still).

Great video btw! I also see a lot, perhaps too many paramecia. What are the protists in the very beginning? I'd say it looks like Oxytricha but thats more or less a guess.
by Wes
Fri Oct 11, 2019 8:06 am
Forum: Microscopes and optics
Topic: Opening Zeiss 47 30 28 Trinocular Head
Replies: 11
Views: 633

Re: Opening Zeiss 47 30 28 Trinocular Head

What is you strategy for cleaning it? Today I am very reluctant to open it but in the past I would spend entire days disassembling, cleaning, reassembling, centering and so on (probably a very bad idea but I guess I learn). Instead I try to maintain low amounts of dust in the room where I keep the m...
by Wes
Thu Oct 10, 2019 11:15 pm
Forum: Microscopes and optics
Topic: Opening Zeiss 47 30 28 Trinocular Head
Replies: 11
Views: 633

Re: Opening Zeiss 47 30 28 Trinocular Head

May I ask what is your end goal here? Cleaning dust or..?
by Wes
Thu Oct 10, 2019 11:14 pm
Forum: Identification help
Topic: Amoeboid infected with parasites?
Replies: 7
Views: 577

Amoeboid infected with parasites?

50x46 µm, found it in a pond water culture. The structure on the left is its nucleus I think but I have no clue what the other 5 round things are (some kind of parasites maybe?)

Image
by Wes
Thu Oct 10, 2019 10:44 pm
Forum: Pictures and Videos
Topic: Gastrotricha
Replies: 5
Views: 448

Re: Gastrotricha

Interesting and sharp video but I admit I know nothing about gastrotrichs. What are they most related to?