Search found 532 matches

by Wes
Wed Jul 08, 2020 7:54 pm
Forum: Illumination Techniques
Topic: Jamin-Lebedeff Interference Contrast Microscopy
Replies: 14
Views: 1000

Re: Jamin-Lebedeff Interference Contrast Microscopy

After searching for a while I finally found a complete Jamin-Lebedeff interference set that I could install on my Phomi. Its a system of condensers and objectives where each pair contains an optically identical crystal used for beam splitting and recombining. A reference beam is compared to a sampl...
by Wes
Tue Jul 07, 2020 6:37 am
Forum: Illumination Techniques
Topic: Jamin-Lebedeff Interference Contrast Microscopy
Replies: 14
Views: 1000

Re: Jamin-Lebedeff Interference Contrast Microscopy

hsi100 wrote:
Sun Jul 05, 2020 10:02 am
Spectacular images.
Any idea where I can get one as it seems out of production?
I did find this paper: https://journals.plos.org/plosone/artic ... ne.0227096
Thanks hsi100,

You can find a JL set on Ebay.
by Wes
Fri Jul 03, 2020 6:37 am
Forum: Pictures and Videos
Topic: Cyanobacteria in Freshwater ponds
Replies: 6
Views: 434

Re: Cyanobacteria in Freshwater ponds

Nice images, especially the first one!
DonSchaeffer wrote:
Fri Jul 03, 2020 2:51 am
What are these? They don't look like any bacteria I've ever seen. Oh I see! They are what I have called "Blue Green Algae." I've seen these a lot.
Blue green algae are cyanobacteria.
by Wes
Wed Jul 01, 2020 7:31 pm
Forum: Pictures and Videos
Topic: Dinoflagellate
Replies: 8
Views: 410

Re: Dinoflagellate

Nice!

Do these contain any pigments?
by Wes
Wed Jul 01, 2020 7:30 pm
Forum: Pictures and Videos
Topic: Acanthocystis
Replies: 5
Views: 387

Re: Acanthocystis

Thank you very much for the kind remarks mintakax and janvangastel.
by Wes
Sun Jun 28, 2020 12:05 pm
Forum: Illumination Techniques
Topic: Epi DIC woes
Replies: 44
Views: 2123

Re: Epi DIC woes

Is the reflector (semi mirror) a first surface mirror? Have you tried flipping the mirror?
by Wes
Sun Jun 28, 2020 8:24 am
Forum: Specimens, samples and slides
Topic: How do you clean your coverslips?
Replies: 5
Views: 277

Re: How do you clean your coverslips?

Thank you everyone for the information and ideas and special thanks to John for showing his mountain. 1. NaOH definitely dissolves glass A long time ago, I used to seed cells on coverslips for immunofluorescence and I clearly remember we used to clean the slips in 2M NaOH (or maybe KOH) and that all...
by Wes
Sat Jun 27, 2020 4:58 pm
Forum: Microscopes and optics
Topic: What to do about coating damage in Ortholux trinoc head
Replies: 4
Views: 183

Re: What to do about coating damage in Ortholux trinoc head

Try a cotton swab moistened with the cleanest water you can find, move it in a circular motion starting from the center and moving to the periphery of the glass surface you want to clean. That may remove a lot of the dirt you see.
by Wes
Sat Jun 27, 2020 4:56 pm
Forum: Specimens, samples and slides
Topic: How do you clean your coverslips?
Replies: 5
Views: 277

How do you clean your coverslips?

Whenever I open a new pack of coverslips they are always dirty (mostly specks but sometimes residue as well). Normally I'd give them an alcohol wipe and that's fine for wet mounts. Sometime ago I made attempts to arrange diatoms on adhesive-coated coverslips. I cleaned them in 2M sodium hydroxide fo...
by Wes
Tue Jun 23, 2020 5:12 pm
Forum: Pictures and Videos
Topic: Look into my eyes! - Nursery web spider with cocon
Replies: 7
Views: 230

Re: Look into my eyes! - Nursery web spider with cocon

Nice shot Bob!

Would you mind posting your setup so I can see how assembled the lens and objective.
by Wes
Sat Jun 20, 2020 4:04 pm
Forum: Pictures and Videos
Topic: Triceratium diatom from Pensacola, USA
Replies: 7
Views: 456

Re: Triceratium diatom from Pensacola, USA

Nice photo! Would be interesting to see a stack of that diatom.
by Wes
Sat Jun 20, 2020 4:03 pm
Forum: Specimens, samples and slides
Topic: A tip and a question about a slide of clean diatoms without silt
Replies: 17
Views: 761

Re: A tip and a question about a slide of clean diatoms without silt

These are two problems I'm currently dealing with. To get rid of the silt and other fine particles two very experienced diatomists told me to get a sieve/mesh (15-20 µm pore size). If the particles are sticking to the diatoms after cleaning or if you get clumps of diatoms as often happens after boil...
by Wes
Thu Jun 18, 2020 8:36 am
Forum: For forum members who want to buy and sell equipment
Topic: Zeiss Universal-gone
Replies: 7
Views: 702

Re: Zeiss Universal

OK, I thought there were three, but you are right, there are four grub screws. If you have no marks to go by, you can still line it up, but it's a longer process. I've been able to take them off, and change them between microscopes. That being said, it can take a little trial and error to get it ju...
by Wes
Wed Jun 17, 2020 8:34 pm
Forum: Illumination Techniques
Topic: Jamin-Lebedeff Interference Contrast Microscopy
Replies: 14
Views: 1000

Re: Jamin-Lebedeff Interference Contrast Microscopy

I thought I'd give a brief description of how the JL microscope actually works. A plane polarized wavefront enters beamsplitter S (in the condenser) where it is split into two orthogonally polarized and laterally displaced rays. These pass a half-wave plate that rotates their vibration plane by 90˚ ...
by Wes
Wed Jun 17, 2020 7:18 pm
Forum: For forum members who want to buy and sell equipment
Topic: Zeiss Universal-gone
Replies: 7
Views: 702

Re: Zeiss Universal

How would you center the optovar on this stand? There are three little centering screws on the bottom of the head. They often each have a retaining screw covering them. Just adjust slowly and carefully. Are you talking about centering of the binocular head port? Because the missing optovar is held ...
by Wes
Wed Jun 17, 2020 6:07 am
Forum: For forum members who want to buy and sell equipment
Topic: Zeiss Universal-gone
Replies: 7
Views: 702

Re: Zeiss Universal

How would you center the optovar on this stand?
by Wes
Tue Jun 16, 2020 7:42 pm
Forum: Miscellaneous
Topic: Mad scientist must have?
Replies: 7
Views: 769

Re: Mad scientist must have?

BramHuntingNematodes wrote:
Tue Jun 16, 2020 7:26 pm
looks like the wheel of a pirate ship!
:lol:
by Wes
Tue Jun 16, 2020 6:59 pm
Forum: Miscellaneous
Topic: Mad scientist must have?
Replies: 7
Views: 769

Re: Mad scientist must have?

That is a Zeiss Linnik-type interference microscope

Image
by Wes
Tue Jun 16, 2020 4:44 pm
Forum: Pictures and Videos
Topic: Frustulia rhomboides (another)
Replies: 2
Views: 354

Re: Frustulia rhomboides (another)

Great work!

Out of curiosity, when you take the photo do you have a green filter in the light path and shoot in B&W?
by Wes
Tue Jun 16, 2020 7:25 am
Forum: Illumination Techniques
Topic: Jamin-Lebedeff Interference Contrast Microscopy
Replies: 14
Views: 1000

Re: Jamin-Lebedeff Interference Contrast Microscopy

Many thanks to everyone for the interest. While I find these really cool from an aesthetic perspective, I’m struggling a bit to see the practical application of this technique. What would this method reveal that others do not? It allows you to measure the phase difference between an object of intere...
by Wes
Mon Jun 15, 2020 10:34 pm
Forum: Illumination Techniques
Topic: Jamin-Lebedeff Interference Contrast Microscopy
Replies: 14
Views: 1000

Jamin-Lebedeff Interference Contrast Microscopy

After searching for a while I finally found a complete Jamin-Lebedeff interference set that I could install on my Phomi. Its a system of condensers and objectives where each pair contains an optically identical crystal used for beam splitting and recombining. A reference beam is compared to a sample...
by Wes
Mon Jun 15, 2020 5:04 pm
Forum: Pictures and Videos
Topic: Acanthocystis
Replies: 5
Views: 387

Re: Acanthocystis

Thank you 75'

I thought its trying the get rid of the little guy, to me it looked like its pushing it away.
by Wes
Mon Jun 15, 2020 1:17 pm
Forum: Beginner's corner
Topic: Help with Microscope?
Replies: 7
Views: 681

Re: Help with Microscope?

For a med student you could get a nice binocular Zeiss Standard with a 2,5 to 100x achromat (or maybe even plan) objectives for about $300. Its all you really need for histological sections (I think thats what they do in med school). Depending on which part of the world you are it could be that some...
by Wes
Mon Jun 15, 2020 12:26 pm
Forum: Pictures and Videos
Topic: Acanthocystis
Replies: 5
Views: 387

Acanthocystis



You can observe the kinetosomes moving back and forth on the axonemes.

Taken with Planapo 63/1,4 with DIC. Depending on your internet connection you can choose 1080p for best video quality.
by Wes
Mon Jun 15, 2020 10:19 am
Forum: Microscopes and optics
Topic: Wave plates on Ebay
Replies: 6
Views: 360

Re: Wave plates on Ebay

by Wes
Sat Jun 13, 2020 5:23 pm
Forum: Pictures and Videos
Topic: flatworm
Replies: 5
Views: 283

Re: flatworm

Video unavailable
This video is private.
by Wes
Fri Jun 12, 2020 6:28 pm
Forum: Pictures and Videos
Topic: First attempts at arranging diatoms
Replies: 39
Views: 1214

Re: First attempts at arranging diatoms

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...
by Wes
Fri Jun 12, 2020 6:09 pm
Forum: Pictures and Videos
Topic: First attempts at arranging diatoms
Replies: 39
Views: 1214

Re: First attempts at arranging diatoms

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...
by Wes
Sat Jun 06, 2020 3:18 pm
Forum: Camera systems and imaging
Topic: Video Editing Software
Replies: 11
Views: 4028

Re: Video Editing Software

I use FFmpeg. Its free and many of the video editors you know are based on it. You can do pretty much anything with it, its really good but has a bit of a steep learning curve.
by Wes
Sat Jun 06, 2020 10:59 am
Forum: Pictures and Videos
Topic: First attempts at arranging diatoms
Replies: 39
Views: 1214

Re: First attempts at arranging diatoms

What about this one? https://www.ebay.co.uk/itm/Bugelmessschraube-0-25-mm-mit-1-Nonius-0-001-DIN-863-Mikrometer/263342487388?hash=item3d506f775c:g:bCcAAOSwenJaGCJe That looks nice, I would like to see the brand though. Good point, not clear what the brand is (may explain the rather accessible price...