MicrobeHunter.com Microscopy Forum

You can read the original (recent) research here:

https://www.cell.com/current-biology/fu ... 19)31431-9

Found the link! "All these photos were taken with the Zeiss lens 1/10 inch, nA 1.63. Monochromatic sunlight. Special compensation eyepiece 12. Condenser 1.6. Inclusion in a medium with refractive index 2.4. Coverslips and flint glass slides with a refractive index of 1.72." Image is in the post abo...

Great Photos 75RR! Maybe you need to try one of the older Zeiss Objectives with n.A. of incredible 1.6: PlanApo 100/1,6 Imm or the 74/1,6 ;) NA of 1,6?? I had never heard of that till now. I know some of the modern 100x planapos used for TIRF microscopy deliver NA of 1,46. Can you provide a bit mor...

Out of curiosity do you see the striae in Amphipleura pellucida with the 63x planapo? It might be the right subject to also test the effect of condenser oiling on resolution.

Thats really good! Excellent contrast and resolution, thanks for sharing.

One minor issue I have is that the replacement dichroic mirror I use does not fit in the round slots and is 1-2 mm above its intended position. Since only the 580 DM fits in the round slot there is a small lateral shift in where the lamp field stop is seen when switching back and forth between the ...

My impression was that it is the most universal setup to have a short pass excitation filter and a long pass emission filter. So that all light below a certain wave length is used for excitation and everything above is passed to the eyepiece. This is how my two setups work now. With my 18W LED I ge...

Nice, the first view reminds of H.P. Lovecraft's Cthulu

For the second one, 546BP and 590LP, a dichroic mirror of 510nm (or 515nm) is inappropriate! You are correct, this would be an inappropriate combination. In this case I used the original DM that came with the condenser which is a FT 580 splitter. I will try a BP 430 exciter for chlorophyll next time.

Are you happy with the filter sets in you condenser? Hi Bob, The condenser came with only two filter sets, one for FITC and one for rhodamine. The dichroic mirror of the FITC set was really fried so I had to replace it with a FT 510 beam splitter and it seems to work ok now. I quite like the use of...

I sampled a small drop of green slime from the bottom of one of my cultures and observed it under epifluorescent conditions. When I illuminate the sample with a bandpass filter of 450-490 nm combined with a longpass filter of 515 nm it seems like the eukaryotic algae light up in red. When I use gree...

Interesting, mrsonchus' posts are being actively removed from this thread.

I'd leave them in bleach for a couple of days.

This seems relevant

https://www.rsc.org/images/analysis-asb ... 214823.pdf

I notice a lot of spammers around here lately. That go me thinking why not give permissions to the regulars around here to decide whether newcomers are to be allowed to post. First posts/threads by new members get inspected by a trusted bunch of selected members with the special permission and decid...

I think the second image shows Licmophora, maybe someone can confirm (75RR probably knows this )

Wes

But doesn't that shed off cotton fibers?

But I was wondering, given the size of this thing, what is a descent dust cover for it? Or do I just keep it under an unrespectful plastic bag? :-) There are these blue cloth bags from Zeiss but using a regular plastic bag is ok. I use one to cover my Phomi, some people claim it can attract dust be...

Maybe you have some ammo for my rifle as well? https://i.pinimg.com/236x/b1/a6/ab/b1a6ab7576b8bd730f9ff510748a822c--art-sculptures-dollhouse-miniatures.jpg Hi Wes, is this from Willard Wigan? Bob Hi Bob, I honestly have no idea. I simply image searched a combination of keywords relevant to this spa...

Maybe you have some ammo for my rifle as well?

OK you've convinced me, I will try it with the peripherally delaminated lens doublet. What proportion of Canada balsam to xylene did you use?

Very interesting Bob. My concern lies with the re-centering of all lens elements in the Planapo but as you said this is the difficult part. I considered doing essentially what you suggest (mixture of xylene and canada balsam) to fix the peripheral delamination of a 10x Kpl eyepiece top lens but wasn...

75RR wrote: ↑

Thu Oct 31, 2019 5:51 pm

Delamination ... Zeiss' Achilles heel

http://www.microscopy-uk.org.uk/mag/ind ... delam.html

Imagine a world without Zeiss delamination...

MicroBob wrote: ↑

Thu Oct 31, 2019 4:38 pm

I will make an attempt to repair them

What is your plan here? This genuinely sounds like a difficult task to say the least.

If by the head tube you mean the tube with the push-rod on the side, don't worry. I'm not opening that anytime soon, but as I understand, removing it as a whole by means of the dovetail is quite innocent (as is for the binocular head)? Exactly, that is not a problem, just be gentle i.e. no bumps, d...

What an aesthetically pleasing shot! Thanks for sharing 75'

Don't use a screwdriver. Don't use the wrong sized screwdriver ;) The world seems to be awash with slotted screwdrivers that are a bad fit! What I'm referring to is to avoid unscrewing things like the prism changer in the tube head, the optovar, the basis of the binocular head. Once you tamper with...

TimVP wrote: ↑

Thu Oct 31, 2019 9:38 am

As I plan to take this machine apart in the future, are there any big don'ts I should know of

Don't use a screwdriver.

which is probably why the microscope was priced the way it was. I suggest you inspect your new scope for delamination as this is a deal breaker in my opinion. You need to take off the binocular head, the tube head with the attached optovar and the nosepiece. The binocular head is very easy to remov...

Superb choice! - Is there an alternative lightsource for the radiatorlike illuminator, like an LED unit? There are LEDs etc but I'd get the complementary light source. You can get the 12V 60W lamphouse or a 12V 100W tungsten halogen lamphouse and a power supply for less than a hundred dollars on eba...