MicrobeHunter.com Microscopy Forum

After searching for a while I finally found a complete Jamin-Lebedeff interference set that I could install on my Phomi. Its a system of condensers and objectives where each pair contains an optically identical crystal used for beam splitting and recombining. A reference beam is compared to a sampl...

hsi100 wrote: ↑

Sun Jul 05, 2020 10:02 am

Spectacular images.
Any idea where I can get one as it seems out of production?
I did find this paper: https://journals.plos.org/plosone/artic ... ne.0227096

Thanks hsi100,

You can find a JL set on Ebay.

Nice images, especially the first one!

DonSchaeffer wrote: ↑

Fri Jul 03, 2020 2:51 am

What are these? They don't look like any bacteria I've ever seen. Oh I see! They are what I have called "Blue Green Algae." I've seen these a lot.

Blue green algae are cyanobacteria.

Nice!

Do these contain any pigments?

Thank you very much for the kind remarks mintakax and janvangastel.

Is the reflector (semi mirror) a first surface mirror? Have you tried flipping the mirror?

Thank you everyone for the information and ideas and special thanks to John for showing his mountain. 1. NaOH definitely dissolves glass A long time ago, I used to seed cells on coverslips for immunofluorescence and I clearly remember we used to clean the slips in 2M NaOH (or maybe KOH) and that all...

Try a cotton swab moistened with the cleanest water you can find, move it in a circular motion starting from the center and moving to the periphery of the glass surface you want to clean. That may remove a lot of the dirt you see.

Whenever I open a new pack of coverslips they are always dirty (mostly specks but sometimes residue as well). Normally I'd give them an alcohol wipe and that's fine for wet mounts. Sometime ago I made attempts to arrange diatoms on adhesive-coated coverslips. I cleaned them in 2M sodium hydroxide fo...

Nice shot Bob!

Would you mind posting your setup so I can see how assembled the lens and objective.

Nice photo! Would be interesting to see a stack of that diatom.

These are two problems I'm currently dealing with. To get rid of the silt and other fine particles two very experienced diatomists told me to get a sieve/mesh (15-20 µm pore size). If the particles are sticking to the diatoms after cleaning or if you get clumps of diatoms as often happens after boil...

OK, I thought there were three, but you are right, there are four grub screws. If you have no marks to go by, you can still line it up, but it's a longer process. I've been able to take them off, and change them between microscopes. That being said, it can take a little trial and error to get it ju...

I thought I'd give a brief description of how the JL microscope actually works. A plane polarized wavefront enters beamsplitter S (in the condenser) where it is split into two orthogonally polarized and laterally displaced rays. These pass a half-wave plate that rotates their vibration plane by 90˚ ...

How would you center the optovar on this stand? There are three little centering screws on the bottom of the head. They often each have a retaining screw covering them. Just adjust slowly and carefully. Are you talking about centering of the binocular head port? Because the missing optovar is held ...

How would you center the optovar on this stand?

BramHuntingNematodes wrote: ↑

Tue Jun 16, 2020 7:26 pm

looks like the wheel of a pirate ship!

That is a Zeiss Linnik-type interference microscope

Great work!

Out of curiosity, when you take the photo do you have a green filter in the light path and shoot in B&W?

Many thanks to everyone for the interest. While I find these really cool from an aesthetic perspective, I’m struggling a bit to see the practical application of this technique. What would this method reveal that others do not? It allows you to measure the phase difference between an object of intere...

After searching for a while I finally found a complete Jamin-Lebedeff interference set that I could install on my Phomi. Its a system of condensers and objectives where each pair contains an optically identical crystal used for beam splitting and recombining. A reference beam is compared to a sample...

Thank you 75'

I thought its trying the get rid of the little guy, to me it looked like its pushing it away.

For a med student you could get a nice binocular Zeiss Standard with a 2,5 to 100x achromat (or maybe even plan) objectives for about $300. Its all you really need for histological sections (I think thats what they do in med school). Depending on which part of the world you are it could be that some...

You can observe the kinetosomes moving back and forth on the axonemes.

Taken with Planapo 63/1,4 with DIC. Depending on your internet connection you can choose 1080p for best video quality.

What about this one?






https://www.ebay.de/itm/MIKROSKOP-KOMPE ... SwimRe3d1u

Video unavailable
This video is private.

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...

I use FFmpeg. Its free and many of the video editors you know are based on it. You can do pretty much anything with it, its really good but has a bit of a steep learning curve.

What about this one? https://www.ebay.co.uk/itm/Bugelmessschraube-0-25-mm-mit-1-Nonius-0-001-DIN-863-Mikrometer/263342487388?hash=item3d506f775c:g:bCcAAOSwenJaGCJe That looks nice, I would like to see the brand though. Good point, not clear what the brand is (may explain the rather accessible price...