Search found 692 matches

by Wes
Thu Jan 21, 2021 7:55 pm
Forum: My microscope
Topic: My Zeiss Photomicroscope III
Replies: 41
Views: 20040

Re: My Zeiss Photomicroscope III

I just picked one of these up, it’s in ok shape.. I can’t for the life of me figure out how to adjust the stage tension, not sure if it’s stuck or not.. it takes both hands to move it up and down... also anyone have resources on restoring these guys? I’ve read the user manuals, but not much help. T...
by Wes
Fri Jan 15, 2021 9:15 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted - Zeiss intermediate rings - and/or dic sliders for standard
Replies: 11
Views: 372

Re: Wanted - Zeiss intermediate rings - and/or dic sliders for standard

75RR wrote:
Fri Jan 15, 2021 8:49 pm
.
Wait, where is the male thread on this ring?
by Wes
Fri Jan 15, 2021 4:01 pm
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 465

Re: Silicone immersion objectives - any experience?

viktor j nilsson wrote:
Fri Jan 15, 2021 12:13 pm
Here's another thought. A 50/50 glycerol/water solution has a RI of 1.398 at 20°C. Maybe that could be a workable substitute for silicone oil?
As far as refractive index goes it sounds fine but the mixture could differ in its dispersion properties relative to the oil.
by Wes
Fri Jan 08, 2021 9:44 am
Forum: Pictures and Videos
Topic: Antique Diatom Arrangement
Replies: 12
Views: 534

Re: Antique Diatom Arrangement

Nice, I like the custom stage job.
by Wes
Thu Jan 07, 2021 9:12 pm
Forum: Pictures and Videos
Topic: Antique Diatom Arrangement
Replies: 12
Views: 534

Re: Antique Diatom Arrangement

Excellent images Kurt. What microscope have you mounted your diverse objectives on?
by Wes
Sun Jan 03, 2021 12:43 pm
Forum: Pictures and Videos
Topic: Chilodonella
Replies: 9
Views: 457

Re: Chilodonella

Very nice video
by Wes
Fri Jan 01, 2021 7:58 pm
Forum: Pictures and Videos
Topic: Botryococcus
Replies: 0
Views: 205

Botryococcus

Image

The one and only time I saw Botryococcus.
by Wes
Fri Jan 01, 2021 12:09 pm
Forum: Identification help
Topic: Unidentified amoeboflagellate
Replies: 2
Views: 204

Re: Unidentified amoeboflagellate

Hello Hans, I think the organism was very stressed (terribly squished) but if I recall correctly I saw a second long flagellum before I started shooting the video. The trailing flagellum-like appendage was some sort of a cytoplasmic strand trailing behind. IDing squished things is generally difficul...
by Wes
Wed Dec 30, 2020 10:11 pm
Forum: Identification help
Topic: Unidentified amoeboflagellate
Replies: 2
Views: 204

Unidentified amoeboflagellate

Any clues what this amoeboflagellate might be?

by Wes
Thu Dec 24, 2020 9:12 pm
Forum: Specimens, samples and slides
Topic: Humidity chamber for extended observations
Replies: 2
Views: 219

Re: Humidity chamber for extended observations

. Neat idea. I have found Slide Mailers work well, though placing the slide inside if it has a large cover slip is a bit more finicky. It was suggested to me to coat the edges of the coverslip with vaseline using a syringe applicator. A small hole is left in the vaseline lining to allow for water a...
by Wes
Thu Dec 24, 2020 12:38 pm
Forum: Specimens, samples and slides
Topic: Humidity chamber for extended observations
Replies: 2
Views: 219

Humidity chamber for extended observations

When I observe water samples I tend to get rid of the specimen after I'm done with the observation. Last night, however, I decided to save the sample as it was very rich in protists and algae. I used a petri dish and a wet napkin to create a very simple humidity chamber in which I deposited the slid...
by Wes
Thu Dec 24, 2020 12:09 pm
Forum: Microscopes and optics
Topic: Amateur microscope for microphotography
Replies: 12
Views: 593

Re: Amateur microscope for microphotography

The Brunel offer is for a KF2 Junior in, from what I can see, a good condition. But I think the Vickers might be real deal in your case. I'd say go for that first, see if you can arrange with the seller to check it out and if you're happy with the performance go for it. For 90 pounds it might well b...
by Wes
Thu Dec 24, 2020 11:07 am
Forum: Microscopes and optics
Topic: Amateur microscope for microphotography
Replies: 12
Views: 593

Re: Amateur microscope for microphotography

Hi Giulio, I would advise you against buying the Zeiss you linked to. All of the objectives have been substituted with generic non-Zeiss objectives and there are signs of damage if you look at the binocular head. For nearly 400 pounds its not a good deal at all. I think this one would be much better...
by Wes
Mon Dec 21, 2020 10:17 am
Forum: Pictures and Videos
Topic: Freshwater Dinoflagellates
Replies: 14
Views: 1125

Re: Freshwater Dinoflagellates

apochronaut wrote:
Sun Dec 20, 2020 11:05 pm
great video.
glad you like it
micro wrote:
Mon Dec 21, 2020 1:23 am
Shouldn't the sem image have a flagella? The photoshop coloring is really good.
It should, at least I saw them with my scope but maybe this particular individual had lost its flagellum.
by Wes
Sun Dec 20, 2020 8:48 pm
Forum: Pictures and Videos
Topic: Freshwater Dinoflagellates
Replies: 14
Views: 1125

Re: Freshwater Dinoflagellates

When I posted the Dinoflagellates on another microforum I was approached by a SEM enthusiast about whether I could send him a sample of the organisms. So I did and sometime later he came back with a bunch of amazing SEM images. Here is one of them. https://www.mikroskopie-forum.de/index.php?action=d...
by Wes
Sun Dec 20, 2020 6:49 pm
Forum: Pictures and Videos
Topic: Algal blooms and their guards
Replies: 6
Views: 336

Re: Algal blooms and their guards

Wow! Extremely good images Håkan.
by Wes
Mon Dec 14, 2020 6:10 pm
Forum: Identification help
Topic: Is this Beggiatoa
Replies: 4
Views: 461

Re: Is this Beggiatoa

Most likely. If you have DF , the sulphur inclusions will iridesce. Identification to some degree depends on the origin of the sample. Proximity to sewage is a common origin. Eutrophicated lake beds. Any aquatic habitat with a high level of biological oxygen depletion, such as a swamp with yearly c...
by Wes
Mon Dec 14, 2020 10:26 am
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 966

Re: Ilex leaf with worm hole - pen ink stain

MicroBob wrote:
Mon Dec 14, 2020 10:20 am
For Fungi Pianese stainig would work: Fungus pink, plant green.
Do you have this stain at your disposal?
by Wes
Mon Dec 14, 2020 9:09 am
Forum: Identification help
Topic: Is this Beggiatoa
Replies: 4
Views: 461

Is this Beggiatoa



Gliding filamentous bacteria, I suspect Beggiatoa maybe someone can confirm.
by Wes
Sun Dec 13, 2020 8:22 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 966

Re: Ilex leaf with worm hole - pen ink stain

Very nice section and image Bob. Have you tried to section sick plants such as ones infected by fungi/bacteria?
by Wes
Wed Dec 09, 2020 12:17 pm
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 740

Re: Question about setting up Zeiss DIC

Hi Wes, do you use the DIC system with the single analyser slide or the newer one with individual analyser slides pe robjective? The old system doesn't allow the use of the full objectives aperture anyway. Zeiss specifically sait to use oil immersion even for the low power objectives. This might be...
by Wes
Wed Dec 09, 2020 7:33 am
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 740

Re: Question about setting up Zeiss DIC

Bob, would you have a rough idea or guidance in immersing the Zeiss condenser to the slide (dry vs. water vs. oil) at something like 25x and 40x magnifications -- or even lower? Does water help? Oil significantly more? If you do the trig calculation for internal reflection at air-glass interfaces y...
by Wes
Tue Dec 08, 2020 4:40 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 777

Re: Experiments with botanic stains

Do you have a link where your microtome and sectioning technique are described?
by Wes
Tue Dec 08, 2020 4:27 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 777

Re: Experiments with botanic stains

Impressive results Bob! Is this a hand section, looks great. Also which component gives the yellow color?
by Wes
Mon Dec 07, 2020 12:20 pm
Forum: Pictures and Videos
Topic: Oamaru Diatoms
Replies: 4
Views: 447

Re: Oamaru Diatoms

Thank you viktor j nilsson and 75RR Would a side view be triangular, similar to Triceratium scitulum in images 7 and 9 below? https://pubs.usgs.gov/of/2012/1163/pages/Plates/46_Singapore.pdf I think it looks more like a coffee table. https://i.pinimg.com/originals/91/da/b1/91dab18ead4dc3c97d5b049166...
by Wes
Mon Dec 07, 2020 10:00 am
Forum: Pictures and Videos
Topic: Oamaru Diatoms
Replies: 4
Views: 447

Oamaru Diatoms

Image
Triceratium favus var quadrata

Image
Stictodiscus californicus var areolata
by Wes
Fri Dec 04, 2020 8:17 am
Forum: Pictures and Videos
Topic: Exploding fluorescent amoeba
Replies: 13
Views: 771

Re: Exploding fluorescent amoeba

Bruce Taylor wrote:
Wed Dec 02, 2020 10:05 pm
As for the ID, my understanding is that is extremely difficult to distinguish most Mayorella species from Korotnevella, unless you have access to SEM.
Thanks Bruce! I once asked Ferry Siemensma about an amoeba I had made a video of and he just said Mayorella.
by Wes
Fri Dec 04, 2020 8:15 am
Forum: Miscellaneous
Topic: stuck PZO binocular head
Replies: 1
Views: 260

Re: stuck PZO binocular head

Amateur, everyone knows you should put in a pressure cooker and then bang it with a hammer.
by Wes
Wed Dec 02, 2020 7:42 pm
Forum: For forum members who want to buy and sell equipment
Topic: 7-objective revolver for WL, Standard (Zeiss)
Replies: 0
Views: 563

7-objective revolver for WL, Standard (Zeiss)

https://www.ebay.de/itm/7-Fach-Wechsel- ... Swne1fx-HM

Found this while browsing ebay, last one I saw sold for a few hundred and I thought someone here might be interested.
by Wes
Wed Dec 02, 2020 5:31 pm
Forum: Pictures and Videos
Topic: Exploding fluorescent amoeba
Replies: 13
Views: 771

Re: Exploding fluorescent amoeba

It came with the microscope, its installed by default. The photo below shows you where its placed on a Universal stand. You might be able to source one at a reasonable price on Ebay (search for KG1 heat absorbing filter). That is interesting. Was going to put it on the field diaphragm housing filte...