MicrobeHunter.com Microscopy Forum

I just picked one of these up, it’s in ok shape.. I can’t for the life of me figure out how to adjust the stage tension, not sure if it’s stuck or not.. it takes both hands to move it up and down... also anyone have resources on restoring these guys? I’ve read the user manuals, but not much help. T...

75RR wrote: ↑

Fri Jan 15, 2021 8:49 pm

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Wait, where is the male thread on this ring?

viktor j nilsson wrote: ↑

Fri Jan 15, 2021 12:13 pm

Here's another thought. A 50/50 glycerol/water solution has a RI of 1.398 at 20°C. Maybe that could be a workable substitute for silicone oil?

As far as refractive index goes it sounds fine but the mixture could differ in its dispersion properties relative to the oil.

Nice, I like the custom stage job.

Excellent images Kurt. What microscope have you mounted your diverse objectives on?

Very nice video

The one and only time I saw Botryococcus.

Hello Hans, I think the organism was very stressed (terribly squished) but if I recall correctly I saw a second long flagellum before I started shooting the video. The trailing flagellum-like appendage was some sort of a cytoplasmic strand trailing behind. IDing squished things is generally difficul...

Any clues what this amoeboflagellate might be?

. Neat idea. I have found Slide Mailers work well, though placing the slide inside if it has a large cover slip is a bit more finicky. It was suggested to me to coat the edges of the coverslip with vaseline using a syringe applicator. A small hole is left in the vaseline lining to allow for water a...

When I observe water samples I tend to get rid of the specimen after I'm done with the observation. Last night, however, I decided to save the sample as it was very rich in protists and algae. I used a petri dish and a wet napkin to create a very simple humidity chamber in which I deposited the slid...

The Brunel offer is for a KF2 Junior in, from what I can see, a good condition. But I think the Vickers might be real deal in your case. I'd say go for that first, see if you can arrange with the seller to check it out and if you're happy with the performance go for it. For 90 pounds it might well b...

Hi Giulio, I would advise you against buying the Zeiss you linked to. All of the objectives have been substituted with generic non-Zeiss objectives and there are signs of damage if you look at the binocular head. For nearly 400 pounds its not a good deal at all. I think this one would be much better...

apochronaut wrote: ↑

Sun Dec 20, 2020 11:05 pm

great video.

glad you like it

micro wrote: ↑

Mon Dec 21, 2020 1:23 am

Shouldn't the sem image have a flagella? The photoshop coloring is really good.

It should, at least I saw them with my scope but maybe this particular individual had lost its flagellum.

When I posted the Dinoflagellates on another microforum I was approached by a SEM enthusiast about whether I could send him a sample of the organisms. So I did and sometime later he came back with a bunch of amazing SEM images. Here is one of them. https://www.mikroskopie-forum.de/index.php?action=d...

Wow! Extremely good images Håkan.

Most likely. If you have DF , the sulphur inclusions will iridesce. Identification to some degree depends on the origin of the sample. Proximity to sewage is a common origin. Eutrophicated lake beds. Any aquatic habitat with a high level of biological oxygen depletion, such as a swamp with yearly c...

MicroBob wrote: ↑

Mon Dec 14, 2020 10:20 am

For Fungi Pianese stainig would work: Fungus pink, plant green.

Do you have this stain at your disposal?

Gliding filamentous bacteria, I suspect Beggiatoa maybe someone can confirm.

Very nice section and image Bob. Have you tried to section sick plants such as ones infected by fungi/bacteria?

Hi Wes, do you use the DIC system with the single analyser slide or the newer one with individual analyser slides pe robjective? The old system doesn't allow the use of the full objectives aperture anyway. Zeiss specifically sait to use oil immersion even for the low power objectives. This might be...

Bob, would you have a rough idea or guidance in immersing the Zeiss condenser to the slide (dry vs. water vs. oil) at something like 25x and 40x magnifications -- or even lower? Does water help? Oil significantly more? If you do the trig calculation for internal reflection at air-glass interfaces y...

Do you have a link where your microtome and sectioning technique are described?

Impressive results Bob! Is this a hand section, looks great. Also which component gives the yellow color?

Thank you viktor j nilsson and 75RR Would a side view be triangular, similar to Triceratium scitulum in images 7 and 9 below? https://pubs.usgs.gov/of/2012/1163/pages/Plates/46_Singapore.pdf I think it looks more like a coffee table. https://i.pinimg.com/originals/91/da/b1/91dab18ead4dc3c97d5b049166...

Triceratium favus var quadrata


Stictodiscus californicus var areolata

Bruce Taylor wrote: ↑

Wed Dec 02, 2020 10:05 pm

As for the ID, my understanding is that is extremely difficult to distinguish most Mayorella species from Korotnevella, unless you have access to SEM.

Thanks Bruce! I once asked Ferry Siemensma about an amoeba I had made a video of and he just said Mayorella.

Amateur, everyone knows you should put in a pressure cooker and then bang it with a hammer.

https://www.ebay.de/itm/7-Fach-Wechsel- ... Swne1fx-HM

Found this while browsing ebay, last one I saw sold for a few hundred and I thought someone here might be interested.

It came with the microscope, its installed by default. The photo below shows you where its placed on a Universal stand. You might be able to source one at a reasonable price on Ebay (search for KG1 heat absorbing filter). That is interesting. Was going to put it on the field diaphragm housing filte...