MicrobeHunter.com Microscopy Forum

Old thread but I am curious how it went with the darkfield experiment? It seems like strange omission by Olympus, not to have a factory-made option for this. After all, darkfield is very useful to have.

Thanks Macro_Cosmos, good tips!

The Labovert comes with a Leitz 10x/0.25 Plan star objective, which is DIC compatible according to what I have read. So, perhaps also this one will work with the one 25x DIC prism. This is of course just an extrapolation from owl capones experiences.

Supplemental condenser lenses lead double lives and end up in strange places. My best acquisition of a needed one was from ebay and came complete with it's 1.4 achromat aplanat condenser. I picked it up on a Sunday morning and had to wait a while because the seller who looked like Keith Moon, espec...

It appears you have the required componnents for DIC The Polarizer, Analyser and the objective prism for the 25X L on the slider. It appears the condenser has a matching prism for the 25x (S25) as well? If so, it is complete for DIC on that one objective. Also, in a Labovert brochure there was this...

Nope, maybe my phrasing was a bit too convoluted :D The thing in your third picture is the polarizer for the illumination side. In the Labovert the upper arm carries the illumination pathway as well as the image going into the head. You can see some of the internal layout in the patent https://pate...

Pete, Charlie & woodytia, thanks for the excellent suggestions! Looks like we have good choices.

I have a Fluovert with DIC/ICT working on the L25 Fluotar and L40 Fluotar. Afaik in this version the condensor only needs the single S25 (Schnittweite 25mm - 25mm working distance) prism and then the objective-side prism sliders for each respective objective. In practice you can also just use eithe...

The seller has then provided many photos of what looks like maybe two different prisms in the condenser turret. However, I can't verify what exactly the other prism(s) are, the seller isn't 'in the know', and, outside the red 'S 25', there are no other stickers/markings on the outer condenser turret...

It appears you have the required componnents for DIC The Polarizer, Analyser and the objective prism for the 25X L on the slider. It appears the condenser has a matching prism for the 25x (S25) as well? If so, it is complete for DIC on that one objective. Thanks Wayne, helpful as ever :-) Yes, the ...

More pics here:

Hello, Does anyone know what exactly look like the DIC/ICT system parts for a Labovert? I am considering a Leitz/Leica (it has both labels on it, so early 90ies I guess) Labovert that seems to have two of the necessary sliders (that go in below the nosepiece), a polarizer slide of some kind that slo...

I need to look up what a strew slide is. I saw some diatom arrangement slides on the web and they might be an option. Depends on how many slides we deem necessary, those things seem to be expensive.

The sand grain tip sounds great!

At my unit we are planning a optical microscopy course. One of the main topics in it would be different contrast techniques, their pros and cons and usage. This is relatively straightforward with stereo microscopes as one can easily use fresh pond/stream/other samples and demonstrate reflected, brig...

The Reichert Polyvar saga continues :) We found a scope in excellent condition for our university unit. It does not have the universal condenser but we can find that one later. The condenser it does have I am unfamiliar with but will get details later. The objectives were marked as polarization comp...

I am wondering about this polarization optic for the Polyvar, please see image below. It goes on the output of transmitted light at the foot of the microscope. I am thinking that it could be used for simple polarization with the universal condenser but I am not sure what else would be needed. Maybe ...

I am glad you found a solution! These old scopes sometimes require us to come up with some tricks. We are getting two additional IMT-2 units in the next few weeks. They are refurbished and come with phase equipment. It's finally time to replace those Soviet Union inverted scopes that have been in a ...

Here it is! https://imgur.com/a/v749xGr https://imgur.com/a/v749xGr I'm really about the darkfield base, adds an extra dimension to the experience. Very useful to check for dust as well in your prep. Now I'm wondering about the trinocular tube, how do I adapt a M43 camera to it? The camera port is ...

You have a nice scope in your hands there! I like the IMT-2 a lot. We have one in our unit and we use it mainly for teaching, plankton for the most part. It was originally equipped with phase and epi-fluorescence, although we never used the fluorescence system. Now I have equipped if for DIC and wil...

I think you might be able to find a Pl Apo for the Wild probably for around 300-400 euros, depending on condition. At least if you have time to wait on deals. If in a hurry, might cost double that. If I recall correctly, the WD for a 1x Pl Apo is around 40-50mm.

stjepo wrote: ↑

Tue Mar 26, 2024 10:43 am

Will this help you?

https://www.manualslib.com/download/208 ... naval.html

Regards

Thanks. That will be of help, yes, even though it doesn't have any of the photos, diagrams etc. Maybe a machine translation from a German original?

Based on what I read of Leitz 600 and 400 series condensers, their intercept distance with the flip-up top is around 1.2mm, please see below. Doesn't this mean that my problems are likely caused by the condenser not reaching a high enough position, close enough to the slide? Please see the photo I ...

On a proper setup, the condenser top lens should be able to nearly touch the slide bottom - within less than a millimeter. At least, to allow oiling the condenser to the slide for oil immersion imaging. However, the geometry of the stage and condenser carrier could allow the top lens to emerge too ...

It's a bitza. Turns out that the stage is not incompatible with the condenser and it's rack. Instead the condenser rack was mounted too low and it was stuck there. There is a thumbscrew on the right side of the condenser rack that, when loosened, let's the condenser mount slide properly up. This re...

It's a bitza. Turns out that the stage is not incompatible with the condenser and it's rack. Instead the condenser rack was mounted too low and it was stuck there. There is a thumbscrew on the right side of the condenser rack that, when loosened, let's the condenser mount slide properly up. This res...

Based on what I read of Leitz 600 and 400 series condensers, their intercept distance with the flip-up top is around 1.2mm, please see below. Doesn't this mean that my problems are likely caused by the condenser not reaching a high enough position, close enough to the slide? Please see the photo I p...

Thanks. I was able to realign the lamp condenser. It is straight now, not tilting. Also made a DIY target from a circular frosted glass filter. I think I was able to center the lamp according to the instructions in the 100Z user manual. Then I tried setting up the 402a Zernike condenser that I have ...

Further investigation revealed that the lamp condenser (to the left from the bulb) seems to be pretty misaligned. Isn't that supposed to be standing straight and not tilted like in the photo?

Hello,

How do I center and adjust the lamp on a Leitz 100Z lamp when there is no adjustment disc?

Disc and procedure shown here:

I agree with Scaro. Plus the darkfield base is a big advantage to have as an option, even if you mostly use gooseneck LEDs. Darkfield really improves things on a number of different subjects, compared to reflected light or transmitted brightfield. In fact, darkfield on a stereo is almost my go-to li...