Search found 443 matches

by microb
Tue Nov 24, 2020 7:44 pm
Forum: Illumination Techniques
Topic: Leitz Wetzlar Gelbgrun Transmission
Replies: 1
Views: 30

Leitz Wetzlar Gelbgrun Transmission

What is the transmission on this filter? For example, does it have a narrow band for phase contrast?

Leitz Wetzlar Gelbgrun
by microb
Tue Nov 24, 2020 8:05 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

I wonder what would happen if I hit the water with parallel beam. More water molecules will be covered. Will it make the counts higher? Will it improve or diminish the spectral resolution? If the counts would get lower or spectral resolution poorer. Then I'd just forget about parallel beams. This p...
by microb
Tue Nov 24, 2020 4:31 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

Given your video, that beam is expanding fast after focusing at 20mm out where it image inverts to widen out. Since this is a light source, image inversion doesn't matter. So odds are, you'll want another short focal length doublet. So if you have an objective you want to use, get the size on that p...
by microb
Tue Nov 24, 2020 4:05 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

So because of the lens at front between the 75mm focal length and the lens is 20mm from focus (the blue housing). Then I can just put a 20mm plano concave lens right at the front of existing lens so the laser can become parallel right? I can't put a 30mm plano concave because it is inside the box. ...
by microb
Sun Nov 22, 2020 9:40 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

viktor j nilsson wrote:
Sun Nov 22, 2020 9:24 pm
:shock:

If that's the "reduced baseplate" I want to see the extended baseplate version...
This is the reduced base plate:
https://www.edinst.com/us/products/rm5- ... icroscope/
by microb
Sun Nov 22, 2020 9:05 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

I have a feeling this machine pictured below is slightly expensive. But I'm just guessing. Cool to see all the options:

https://www.edinst.com/us/products/rms1 ... icroscope/
by microb
Sun Nov 22, 2020 8:58 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

Without the front attachment, the lens is almost in the edge of the ring. So I need to find a plano concave lens with focal length of 20mm. Here seems to be one. https://www.amazon.com/Diameter-Plano-c ... B013SL7W08 Why is 20mm plano concave lens so hard to find? I just saw this mixed in among the...
by microb
Sun Nov 22, 2020 8:31 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

So Raman spectrometer is overpriced? But note the $15,000 seems to be the cheapest. The Thermo Fisher RMX handheld is being sold for more than $25,000. For DIY, I'm assuming a cheapo laser. So maybe that's where the extra parts costs are. So you could have what a Leica confocal system has in it: st...
by microb
Sun Nov 22, 2020 5:23 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

Wait. The focal length of my Raman is 75mm. What I meant by 20mm was the distance from edge of ring to sample. But the focal length is 75mm as shown in the following specification. How would it change the above? Would it become easier or harder? Thanks. Probably easier since you can probably find a...
by microb
Sun Nov 22, 2020 2:00 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

My raman has converging laser focused 20mm from front of lens. Does anyone know what kind of lens I should use to convert it to parallel (or whatever required by microscope)? Thanks for the info on the Raman set up. For lens assembly, you want to take light that is focused 20mm from your existing s...
by microb
Sat Nov 21, 2020 7:12 am
Forum: Camera systems and imaging
Topic: Olympus DP70
Replies: 11
Views: 133

Re: Olympus DP70

I think you need one of these: https://www.ebay.com/itm/OLYMPUS-DP70-AQ8221-DV492702-AQ8222-DIGITAL-MICROSCOPE-CAMERA-PCI-INTERFACE-CARD/333763985117?hash=item4db5e23edd:g:iSQAAOSwmFNfky0A https://www.ebay.com/itm/OLYMPUS-DP70-AQ8221-DV492702-AQ8222-DIGITAL-MICROSCOPE-CAMERA-PCI-INTERFACE-CARD/29348...
by microb
Fri Nov 20, 2020 6:57 pm
Forum: Microscopy accessories
Topic: Raman Spectrometer 1um to 20um IR
Replies: 1
Views: 72

Re: Raman Spectrometer 1um to 20um IR

What sensors are used for a Raman spectrometer to get data in situ for the longer frequencies? A chip like the TCD1304AP gets 300nm ~ 1100nm. There are some chips I found that go to 3000nm (3um). (https://www.hamamatsu.com/resources/pdf/ssd/g11508_etc_kmir1032e.pdf) The only other way seems to be u...
by microb
Fri Nov 20, 2020 12:12 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

I still don't understand how wavenumber is supposed to make comparable numbers independent of the nm laser used. Raman spectroscopy looks at the shift between the input wavelength and what bounces back, which is independent of input wavelength. So what you're graphing is the spectrum of the raman s...
by microb
Fri Nov 20, 2020 4:12 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

4. Lastly. Where to buy a cheap VIS/NIR IR spectroscope? Company such as Stellernet is selling a package for $3000. Maybe there is one sub $1000? I need an IR spectrometer in addition to my existing Raman Spectrometer. What range do you need for the first and then what range does the Raman get you?...
by microb
Fri Nov 20, 2020 4:09 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

Here's an affordable spectrometer that claims to get to 920nm into the near IR range: https://www.ebay.com/itm/Spectrometer-Spectrometre-Spektrometre-with-SMA-connector-Extended-Range-920nm/283947908442?hash=item421c9d3d5a:g:VSkAAOSwGmteke1h That's the ThunderOptics guys in France. The software is ...
by microb
Fri Nov 20, 2020 1:03 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

I bought the 532nm Raman spectrometer last year for $15,000. Here is the front of it. I measured the ring to be about 44.5mm so I ordered a Kodak series V-VI step up ring at ebay to get use of the VI size. I will get it next week. If it fits, that's the time I'll get a microscope to use the sample ...
by microb
Fri Nov 20, 2020 12:54 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

4. Lastly. Where to buy a cheap VIS/NIR IR spectroscope? Company such as Stellernet is selling a package for $3000. Maybe there is one sub $1000? I need an IR spectrometer in addition to my existing Raman Spectrometer. What range do you need for the first and then what range does the Raman get you?...
by microb
Thu Nov 19, 2020 8:31 pm
Forum: Microscopy accessories
Topic: Raman Spectrometer 1um to 20um IR
Replies: 1
Views: 72

Raman Spectrometer 1um to 20um IR

What sensors are used for a Raman spectrometer to get data in situ for the longer frequencies? A chip like the TCD1304AP gets 300nm ~ 1100nm. There are some chips I found that go to 3000nm (3um). (https://www.hamamatsu.com/resources/pdf/ssd/g11508_etc_kmir1032e.pdf) The only other way seems to be us...
by microb
Thu Nov 19, 2020 6:47 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

I saw the same style of sample stage at amazon where the neck is continuous to the binocular head. I think it is standard. My question is whether there are models where the upper part of the neck can be separated from the lower part. Here's one and it's an Olympus: https://www.ebay.com/itm/McBain-I...
by microb
Thu Nov 19, 2020 6:41 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

If I'll just buy a whole microscope and do the same conversion. If I cut the middle neck. How do I attach the top plate to the lower neck? I have a ring (the same as the golden one in original picture so no problem fabricating the plate). Problem is how to connect the sawed neck at middle (let's sa...
by microb
Thu Nov 19, 2020 6:37 pm
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

portbon wrote:
Thu Nov 19, 2020 8:37 am
The manufacturer is selling me the black sample stage for $1000.
What's a "sample stage" in this context? You're talking just the item shown minus the black box that sat on top, right?
by microb
Thu Nov 19, 2020 8:25 am
Forum: Microscopes and optics
Topic: What microscope has this style?
Replies: 56
Views: 715

Re: What microscope has this style?

The base, illumination, and stage look somewhat like an Omax or another fairly low cost Chinese scope. You might check "Omax" as an example in the $200 to $300 price range. The upright (beginning about 20mm above the stage height) and the head support (held by 3 socket head screws) don't look famil...
by microb
Mon Nov 16, 2020 10:07 pm
Forum: Microscopes and optics
Topic: Leica DMR Magnifier Lense and TL Lenses
Replies: 2
Views: 96

Re: Leica DMR Magnifier Lense and TL Lenses

Ok found a page that seems to say that the non-Bertrand lens above is a 200mm 1x Telan lens with corrections.
by microb
Mon Nov 16, 2020 10:02 pm
Forum: Microscopes and optics
Topic: Leica DMR Magnifier Lense and TL Lenses
Replies: 2
Views: 96

Re: Leica DMR Magnifier Lense and TL Lenses

The manual isn't helpful on confirming TL or not for 15 versus 16:
by microb
Mon Nov 16, 2020 9:57 pm
Forum: Microscopes and optics
Topic: Leica DMR Magnifier Lense and TL Lenses
Replies: 2
Views: 96

Leica DMR Magnifier Lense and TL Lenses

Regarding Leica DMR's, the lenses above the filter cubes, (typically where a Bertrand lens is located) is there aberration correction being done there? Or is there any correction in the tube lens in the head? I'm assuming that the DMR trinocular head has the tube lens. Basically, can I replace the h...
by microb
Mon Nov 16, 2020 8:34 pm
Forum: Home-made microscope adaptations
Topic: Homemade phase annuli
Replies: 13
Views: 2531

Re: Homemade phase annuli

This is something I would like to try out, so if someone else has done so please let me know. If you double tap glass to the build plate of a resin 3D printer, then zero it to include the extra thickness of the glass and tape; a black resin print of a phase contrast annuli could be layered onto the ...
by microb
Sat Nov 14, 2020 9:51 pm
Forum: Pictures and Videos
Topic: Another Bursting
Replies: 8
Views: 254

Re: Another Bursting

So does this mean that an IR or UV filter is needed to not cook the microbe? Or just dim the light more until the bursting stop?
by microb
Sat Nov 14, 2020 9:40 pm
Forum: Digital processing
Topic: Filtering main light source vs post processing of RGB channels.
Replies: 8
Views: 579

Re: Filtering main light source vs post processing of RGB channels.

Filtering light at source with a green filter vs using the manufacturers light source as intended and extracting the green layer from the RBG information post RAW Image? I don't know the images or light source, but camera sensitivity has green overlapping blue and red. So if the incoming data is ba...
by microb
Sat Nov 14, 2020 8:56 pm
Forum: Microscopes and optics
Topic: Balplan Bertrand Lens
Replies: 1
Views: 162

Re: Balplan Bertrand Lens

Found a drawing in a price listing brochure: