Search found 193 matches

by Sauerkraut
Tue Oct 08, 2019 5:42 pm
Forum: Pictures and Videos
Topic: Three Ciliates
Replies: 12
Views: 609

Re: Three Ciliates

Excellent.
by Sauerkraut
Fri Oct 04, 2019 8:13 pm
Forum: Specimens, samples and slides
Topic: Tried glycerin to slow down fast movers
Replies: 36
Views: 5136

Re: Tried glycerin to slow down fast movers

Here’s a YouTube link that compares methylcellulose to physillium seed husk. https://m.youtube.com/watch?v=vowfgDu8Fz4 Think I’ll give the seed husks a try and see how that goes. Heather Heather-- I just came across this thread. Just in case anyone might still be reading it..... Did you ever try ou...
by Sauerkraut
Wed Oct 02, 2019 3:07 pm
Forum: Pictures and Videos
Topic: Festuca Grass Hand Sections
Replies: 23
Views: 704

Re: Festuca Grass Hand Sections

Your work is so beautiful. Thank you for sharing them as well as the stain solutions.

I could easily see such images in textbooks and wall posters.
by Sauerkraut
Mon Sep 30, 2019 6:09 pm
Forum: Beginner's corner
Topic: cheap microscope for identifying soil life?
Replies: 5
Views: 254

Re: cheap microscope for identifying soil life?

...However, there are a great number of soil organisms that are much larger; fungi, arachnids, insects, nematodes,arthropods , amongst others and they all play a role, along with the smaller inhabitants. You can get a stereo microscope for under 100.00 with free shipping to Australia. That will get...
by Sauerkraut
Mon Sep 30, 2019 4:01 pm
Forum: Pictures and Videos
Topic: Colurella sp
Replies: 14
Views: 425

Re: Colurella sp

That's a really nice side view of Colurella. Excellent detail.

I bet you could get nice and differing images from that one waterhole location indefinitely.
by Sauerkraut
Fri Sep 27, 2019 3:54 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Re: Upgrading a Nikon E Plan PH3 Objective

I have a 40x plan objective so don't use the e-plan for BF. But I'll do a comparison of the two objectives in BF anyway. The PC itself seems decent. The specimen, underwhelming. It may be my stubbornness in using deep well slides to keep from crushing the 'elders.' I started a batch of yeast so I c...
by Sauerkraut
Fri Sep 27, 2019 3:49 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Re: Upgrading a Nikon E Plan PH3 Objective

Heather, a few thoughts: 1) Can I assume your phase telescope shows that the phase rings are perfectly aligned? Everything clean? Specimens under a cover slip? The E plan Nikon 40x should give a decent image. I've also had a bad example, used, with no obvious external damage. 2) On the condenser ir...
by Sauerkraut
Fri Sep 27, 2019 3:42 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Re: Upgrading a Nikon E Plan PH3 Objective

At first, I thought that I would be quite content with the E plans, and got myself a set of 4x, 10x, 40x and 100x E plan. But I never liked them, and I found the 40x E plan to be especially poor. Especially in terms of lateral chromatic aberration, but I also found the resolution to be underwhelmin...
by Sauerkraut
Thu Sep 26, 2019 8:14 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Re: Upgrading a Nikon E Plan PH3 Objective

I have a 40x plan objective so don't use the e-plan for BF. But I'll do a comparison of the two objectives in BF anyway. The PC itself seems decent. The specimen, underwhelming. It may be my stubbornness in using deep well slides to keep from crushing the 'elders.' I started a batch of yeast so I ca...
by Sauerkraut
Thu Sep 26, 2019 6:54 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Re: Upgrading a Nikon E Plan PH3 Objective

Thank you Apochronaut, especially for clarification of the DL designation. I'll try to take a closer look and post images in the next few days, time permitting. One thing I'm not clear on is whether the condenser diaphragm should be mostly open for extra light or mostly closed for extra contrast. I'...
by Sauerkraut
Thu Sep 26, 2019 4:09 pm
Forum: Microscopes and optics
Topic: Upgrading a Nikon E Plan PH3 Objective
Replies: 11
Views: 377

Upgrading a Nikon E Plan PH3 Objective

The Optiphot has a Nikon E Plan 40/0.65 160/0.17 PH3 DL objective. It doesn't produce the best image and I'm interested in upgrading. This is the safest choice, as it would directly match the other 20x PH2 and 40x objectives on the scope (not at that asking price though): https://www.ebay.com/itm/14...
by Sauerkraut
Wed Sep 25, 2019 4:10 pm
Forum: Pictures and Videos
Topic: Flagellates and Ciliates
Replies: 18
Views: 421

Re: Flagellates and Ciliates

Nice video and DIC.

Could that long, thin protist be Spirostomum?
by Sauerkraut
Wed Sep 25, 2019 3:24 am
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

I'm sure you are getting good at IDing amoebas. You seem dedicated and patient.

On the camera - can't decide. New or used. Full frame or APS-C. People here are delusional about pricing on their used cameras, at least on craigslist, but the right deal will come.
by Sauerkraut
Tue Sep 24, 2019 3:57 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

Possibly Korotnevella genus but there's no information on the long 'fishing pole.' It doesn't seem like a pseudopodia, based on the visible internal retracting structure. Maybe it's uncharacterized - a new or undescribed species? The SEMs on that site of the Korotnovella species are amazing. There's...
by Sauerkraut
Sun Sep 22, 2019 3:17 pm
Forum: Introduce yourself
Topic: Hello from the Pharaohs' land , Egypt
Replies: 3
Views: 241

Re: Hello from the Pharaohs' land , Egypt

Hello and welcome. In my limited experience - working for a pharmaceutical company for 5+ years - I never even saw a microscope while working with soil cultures. In retrospect, that seems a bit surprising. In grad school, we used them very rarely and just to check on a culture. But that said, there ...
by Sauerkraut
Sun Sep 22, 2019 3:12 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

Thanks, Wes. Much appreciated.
by Sauerkraut
Thu Sep 19, 2019 3:25 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

Thanks for the great amoeba links, Wes.

Do you use Live View to control vibration issues? The SL2 has Live View but I'm not seeing anything for electronic front curtain shutter in the manual. Some posts suggests the SL2 has it but I'll check the photog sites as you suggest.
by Sauerkraut
Wed Sep 18, 2019 3:05 pm
Forum: Identification help
Topic: Ciliate ID
Replies: 5
Views: 817

Re: Ciliate ID

Now that's a very nice image, as compared with my amoeba. Taken with the Canon 50D?
by Sauerkraut
Wed Sep 18, 2019 2:55 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

Here's a raw/uncropped image that better shows the internal structure. To me it looks like it makes a large loop inside the organism.
by Sauerkraut
Wed Sep 18, 2019 2:42 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Re: Cool amoeba

Thanks Wes. Still, there's much more room for improvement over my hand wavering above the 23mm eyepiece. The phase contrast is exciting tho. I like the method a lot. That black tube was being slowly extended and retracted out from the amoeba and it happened multiple times. Internal structure of it w...
by Sauerkraut
Tue Sep 17, 2019 9:01 pm
Forum: Identification help
Topic: Cool amoeba
Replies: 12
Views: 1359

Cool amoeba

Still using my cell phone so not worthy of posting in the Pics/Video section, but here is a really interesting amoeba discovered today while playing with phase contrast. The long black tube was completely retracted at times. Sample is from a fresh water pond. Possibly looks like Dinamoeba (guessing ...
by Sauerkraut
Mon Sep 16, 2019 5:22 pm
Forum: Identification help
Topic: No idea I
Replies: 5
Views: 1078

Re: No idea I

Thank you for posting this link 75RR. It looks excellent - I've been amazed at the level of detail some of these older ID texts offer.
by Sauerkraut
Fri Sep 13, 2019 3:49 pm
Forum: Resources (online, books etc.)
Topic: organisms from the deepest known water on earth.
Replies: 3
Views: 796

Re: organisms from the deepest known water on earth.

Thanks for sharing, apochronaut. The DNA analysis should be really interesting. It's mysterious how tenacious life is - that any possible niche that can be exploited will be exploited, even at great extremes. Maybe finding life on a place like Titan (perhaps under the surface where it's presumably w...
by Sauerkraut
Fri Sep 13, 2019 3:16 pm
Forum: Microscopy accessories
Topic: Patch Stops
Replies: 4
Views: 464

Re: Patch Stops

Hopefully you can share some images and techniques if you get some good results with the oblique patches.

I only had intermittent and fleeting luck with my Chinese scope with the oblique patches. But maybe the Optiphot will perform better since it already gives better dark field.
by Sauerkraut
Fri Sep 13, 2019 3:10 pm
Forum: Off Topic
Topic: Why is Earth so biologically diverse? Mountains hold the answer
Replies: 3
Views: 853

Re: Why is Earth so biologically diverse? Mountains hold the answer

Thanks for sharing this - very interesting. I wonder if microbial diversity is higher in these mountains as well, and perhaps provides at least part of the foundation for the more diverse macro-sized species. Or maybe there are just higher concentrations of microbes assisting in nutrient cycling, sy...
by Sauerkraut
Thu Sep 12, 2019 2:45 pm
Forum: My microscope
Topic: My Zeiss Axiomat
Replies: 8
Views: 923

Re: My Zeiss Axiomat

You weren't kidding microb. That's a serious maze of prisms.

wstenberg, hopefully you'll keep us updated on all of the cool things this scope has to offer, and post some images of pond life from the many optics pathways available.
by Sauerkraut
Wed Sep 11, 2019 8:57 pm
Forum: Pictures and Videos
Topic: Scum grazing rotifers
Replies: 2
Views: 294

Re: Scum grazing rotifers

That's a lot of rotifers and an interesting technique, yielding a very nice image. I'll have to research the lambda plate.
by Sauerkraut
Wed Sep 11, 2019 8:53 pm
Forum: Home-made microscope adaptations
Topic: DIY Phase centering telescope on the cheap
Replies: 8
Views: 938

Re: DIY Phase centering telescope on the cheap

Thanks 75RR - I saw this link, but didn't have the materials on hand to make something similar. Instead I used an old post from this forum on a DIY centering scope that used two eye pieces and a homemade tube. It didn't work, in part due to impatience. As it turns out, with the eyepiece out and no p...
by Sauerkraut
Wed Sep 11, 2019 3:31 pm
Forum: Home-made microscope adaptations
Topic: DIY Phase centering telescope on the cheap
Replies: 8
Views: 938

Re: DIY Phase centering telescope on the cheap

Thank you for your interested farnsy and BillT, First image - I removed the 2x lens from the bottom of the unit. Possibly you could leave it in. Second image - From a previous failed attempt at making a telescope, I had fabricated a disc with a 3mm hole in the middle and glued it onto a Nikon CFW ey...
by Sauerkraut
Tue Sep 10, 2019 3:19 pm
Forum: Home-made microscope adaptations
Topic: DIY Phase centering telescope on the cheap
Replies: 8
Views: 938

DIY Phase centering telescope on the cheap

I bought one of these on eBay, wondering if it could be used for a photo eyepiece (thinking not really because the lens is at the bottom) but discovered that by removing the lens (unscrews) and adding a black disc with a 3 mm hole to the top (drop it down inside from the top), you get a phase center...