Search found 211 matches
- Fri Mar 20, 2020 3:00 pm
- Forum: Microscopes and optics
- Topic: Fluor vs Plan Fluor?
- Replies: 6
- Views: 5036
Re: Fluor vs Plan Fluor?
Wow, this is a lot of great information from everyone. Many thanks. It's going to take me a bit to re-read and digest, but sounds like either one would ultimately be fine. I just hadn't seen an objective with the plan designation along with the fluor. As for the correction collar - I have a few obje...
- Wed Mar 18, 2020 5:13 pm
- Forum: Microscopes and optics
- Topic: Fluor vs Plan Fluor?
- Replies: 6
- Views: 5036
Fluor vs Plan Fluor?
I'm curious what the difference is between these 2 objectives as far as optics quality for my Optiphot: Fluor 40x ph3 DL: https://www.ebay.com/itm/Nikon-Fluor-40x-NA-0-85-Ph3DL-Objective-Lens-w-Correction-Collar/153573900238?hash=item23c1b7bfce:g:3BEAAOSwRwddM0ik and Plan Fluor 40 ph3 DLL: https://w...
- Sun Dec 15, 2019 5:31 pm
- Forum: Miscellaneous
- Topic: Protist behavioral complexity
- Replies: 8
- Views: 6631
Re: Protist behavioral complexity
This isn't 'decision making' a la a neural network, like that of multicelled creatures, but certainly could seem like one to an observer... I don't believe anyone is suggesting that Stentor and other protists are using a neural network to make cognitive decisions. But to me the findings are interes...
- Thu Dec 12, 2019 4:24 pm
- Forum: Pictures and Videos
- Topic: Ascorbic acid
- Replies: 2
- Views: 2405
Re: Ascorbic acid
I like the contrast of the B&W wrinkles versus the smoother peacock dots of color. Very pretty.
- Thu Dec 12, 2019 4:21 pm
- Forum: Beginner's corner
- Topic: deep well microscope slides
- Replies: 6
- Views: 3183
Re: deep well microscope slides
As you note, deep well slides are beneficial for not restricting and squishing larger organisms, which is why I usually use them. But I believe using deep well slides often lowers overall resolution for photos and video. I've made a fool of myself quite uniformly throughout life, so I must be pretty...
- Mon Dec 09, 2019 7:06 pm
- Forum: Miscellaneous
- Topic: Protist behavioral complexity
- Replies: 8
- Views: 6631
Re: Protist behavioral complexity
I posted this from my phone yesterday, not noticing the link to the book Behavior of the Lower Organisms. That's a very interesting book - worth a read for understanding protist behaviors. From my observational experience, the protists move with a certain intelligence as opposed to random oblivion. ...
- Mon Dec 09, 2019 2:22 am
- Forum: Miscellaneous
- Topic: Protist behavioral complexity
- Replies: 8
- Views: 6631
Protist behavioral complexity
https://www.sciencenews.org/article/sin ... mplex-ways
Protists might actually be smarter than some humans in my jaded estimation.
Protists might actually be smarter than some humans in my jaded estimation.
- Sun Nov 24, 2019 7:47 pm
- Forum: Beginner's corner
- Topic: Nikon Optiphot replacement bulb
- Replies: 9
- Views: 5002
Re: Nikon Optiphot replacement bulb
That's good to know. Thank you. My current Optiphot has a horizontal filament so it will be interesting to compare the two bulb varieties under various conditions.abednego1995 wrote: ↑Sun Nov 24, 2019 12:58 amYou’ll need a vertical facing filament to fill the aperture at high numerical apertures.
- Sat Nov 23, 2019 4:44 pm
- Forum: Miscellaneous
- Topic: Snowflakes
- Replies: 1
- Views: 3041
Re: Snowflakes
Thanks for posting this, MichaelG. Scientific and poetic wisdom from a time when 'snowflake' was not a derogatory term.
- Sat Nov 23, 2019 4:24 pm
- Forum: Beginner's corner
- Topic: Nikon Optiphot replacement bulb
- Replies: 9
- Views: 5002
Re: Nikon Optiphot replacement bulb
Thank you, 75RR. That's an interesting observation about the vertical filament. I'll have to investigate the merits of the vertical vs horizontal filaments.
- Fri Nov 22, 2019 5:08 pm
- Forum: Beginner's corner
- Topic: Nikon Optiphot replacement bulb
- Replies: 9
- Views: 5002
Nikon Optiphot replacement bulb
I'm looking for a 12v 50w halogen bulb for my second Optiphot lamp house, since I seem to have a problem saying 'no' to more microscopes. The ones on eBay are a bit pricey (~ $26.00) and don't disclose the pin base specs: https://www.ebay.com/itm/REPLACEMENT-BULB-FOR-NIKON-OPTIPHOT-HALOGEN-50W-12V/1...
- Tue Nov 19, 2019 7:00 pm
- Forum: Pictures and Videos
- Topic: Navicula lyra
- Replies: 15
- Views: 7286
Re: Navicula lyra
That is beautiful, 75RR.
- Tue Nov 19, 2019 4:21 pm
- Forum: Illumination Techniques
- Topic: Plan apo and dark field
- Replies: 4
- Views: 4277
Re: Plan apo and dark field
Thanks, all. I'll try a larger stop, probably in a dedicated DF condenser.
Oil would be a good idea too, but due to the clean up required, I've shied away from using it much.
Oil would be a good idea too, but due to the clean up required, I've shied away from using it much.
- Tue Nov 19, 2019 1:28 am
- Forum: Illumination Techniques
- Topic: Plan apo and dark field
- Replies: 4
- Views: 4277
Plan apo and dark field
My Optiphot has a phase contrast condenser with a dark field stop. The regular plan objectives give decent dark field through 20x with that single DF setting. Today I tried the setup with a 20x plan apo objective and discovered that it would not give DF. Instead it gave oblique illumination that loo...
- Mon Nov 18, 2019 9:45 pm
- Forum: Specimens, samples and slides
- Topic: Disturbing occurrences lately
- Replies: 9
- Views: 8447
Re: Disturbing occurrences lately
This will be my first winter observing microbes in the pond, so it will be interesting to see how the organisms adapt to the cold. Today was supposed to be my day to play with the microscope, but instead someone decided to dump two kittens in front of my house. They promptly lodged themselves inside...
- Thu Nov 07, 2019 8:44 pm
- Forum: Pictures and Videos
- Topic: Micro hedgehog
- Replies: 15
- Views: 7310
Re: Micro hedgehog
Thanks, Bob. Definitely worth a try.
- Thu Nov 07, 2019 5:54 pm
- Forum: Pictures and Videos
- Topic: Micro hedgehog
- Replies: 15
- Views: 7310
Re: Micro hedgehog
Yesterday we let him run around a bit and he tried to bite my wife, opening his jaws as wide as he could! :lol: Apart from the biting he is quite relaxed and doesn't seem to be too stressed by his imprisonment. This thread gave me a good laugh. Overall, the hedgehog still seems like a better guest ...
- Fri Nov 01, 2019 3:58 pm
- Forum: Specimens, samples and slides
- Topic: Disturbing occurrences lately
- Replies: 9
- Views: 8447
Re: Disturbing occurrences lately
I'm going to try and let a greater amount of sample water warm up in the hopes that this will be a gradual warm up as opposed to a drop on a slide. I've noticed something similar lately and am interested in your findings on this warming method. It also seems like there's less protist diversity in p...
- Tue Oct 08, 2019 5:42 pm
- Forum: Pictures and Videos
- Topic: Three Ciliates
- Replies: 12
- Views: 5803
Re: Three Ciliates
Excellent.
- Fri Oct 04, 2019 8:13 pm
- Forum: Specimens, samples and slides
- Topic: Tried glycerin to slow down fast movers
- Replies: 36
- Views: 25895
Re: Tried glycerin to slow down fast movers
Here’s a YouTube link that compares methylcellulose to physillium seed husk. https://m.youtube.com/watch?v=vowfgDu8Fz4 Think I’ll give the seed husks a try and see how that goes. Heather Heather-- I just came across this thread. Just in case anyone might still be reading it..... Did you ever try ou...
- Wed Oct 02, 2019 3:07 pm
- Forum: Pictures and Videos
- Topic: Festuca Grass Hand Sections
- Replies: 24
- Views: 11419
Re: Festuca Grass Hand Sections
Your work is so beautiful. Thank you for sharing them as well as the stain solutions.
I could easily see such images in textbooks and wall posters.
I could easily see such images in textbooks and wall posters.
- Mon Sep 30, 2019 6:09 pm
- Forum: Beginner's corner
- Topic: cheap microscope for identifying soil life?
- Replies: 5
- Views: 5021
Re: cheap microscope for identifying soil life?
...However, there are a great number of soil organisms that are much larger; fungi, arachnids, insects, nematodes,arthropods , amongst others and they all play a role, along with the smaller inhabitants. You can get a stereo microscope for under 100.00 with free shipping to Australia. That will get...
- Mon Sep 30, 2019 4:01 pm
- Forum: Pictures and Videos
- Topic: Colurella sp
- Replies: 14
- Views: 5997
Re: Colurella sp
That's a really nice side view of Colurella. Excellent detail.
I bet you could get nice and differing images from that one waterhole location indefinitely.
I bet you could get nice and differing images from that one waterhole location indefinitely.
- Fri Sep 27, 2019 3:54 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Re: Upgrading a Nikon E Plan PH3 Objective
I have a 40x plan objective so don't use the e-plan for BF. But I'll do a comparison of the two objectives in BF anyway. The PC itself seems decent. The specimen, underwhelming. It may be my stubbornness in using deep well slides to keep from crushing the 'elders.' I started a batch of yeast so I c...
- Fri Sep 27, 2019 3:49 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Re: Upgrading a Nikon E Plan PH3 Objective
Heather, a few thoughts: 1) Can I assume your phase telescope shows that the phase rings are perfectly aligned? Everything clean? Specimens under a cover slip? The E plan Nikon 40x should give a decent image. I've also had a bad example, used, with no obvious external damage. 2) On the condenser ir...
- Fri Sep 27, 2019 3:42 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Re: Upgrading a Nikon E Plan PH3 Objective
At first, I thought that I would be quite content with the E plans, and got myself a set of 4x, 10x, 40x and 100x E plan. But I never liked them, and I found the 40x E plan to be especially poor. Especially in terms of lateral chromatic aberration, but I also found the resolution to be underwhelmin...
- Thu Sep 26, 2019 8:14 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Re: Upgrading a Nikon E Plan PH3 Objective
I have a 40x plan objective so don't use the e-plan for BF. But I'll do a comparison of the two objectives in BF anyway. The PC itself seems decent. The specimen, underwhelming. It may be my stubbornness in using deep well slides to keep from crushing the 'elders.' I started a batch of yeast so I ca...
- Thu Sep 26, 2019 6:54 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Re: Upgrading a Nikon E Plan PH3 Objective
Thank you Apochronaut, especially for clarification of the DL designation. I'll try to take a closer look and post images in the next few days, time permitting. One thing I'm not clear on is whether the condenser diaphragm should be mostly open for extra light or mostly closed for extra contrast. I'...
- Thu Sep 26, 2019 4:09 pm
- Forum: Microscopes and optics
- Topic: Upgrading a Nikon E Plan PH3 Objective
- Replies: 11
- Views: 6980
Upgrading a Nikon E Plan PH3 Objective
The Optiphot has a Nikon E Plan 40/0.65 160/0.17 PH3 DL objective. It doesn't produce the best image and I'm interested in upgrading. This is the safest choice, as it would directly match the other 20x PH2 and 40x objectives on the scope (not at that asking price though): https://www.ebay.com/itm/14...
- Wed Sep 25, 2019 4:10 pm
- Forum: Pictures and Videos
- Topic: Flagellates and Ciliates
- Replies: 18
- Views: 7205
Re: Flagellates and Ciliates
Nice video and DIC.
Could that long, thin protist be Spirostomum?
Could that long, thin protist be Spirostomum?