MicrobeHunter.com Microscopy Forum

The Polyvar Met offered a 2x objective as well as A 1.25 objective that is 45mm parfocal and designed for RL light applications The cat number is 248101 There could a a 9 in front of the 2 depending on the publication date of the literature. FYI the 1.25 is cat number 245105 also with a 9 infront of...

It is interesting that the new greenough platform falls completely away from any recognizable styling of earlier models. If you take a look at the S7E and the A60, there is a distinct swoop along the side that mimics the angularity of the CMO M series viewing tubes. I agree these new bodies are less...

All comment from SuiGenerisBrewomg are spot on. His last point about reaching out to the manufacturers is extremely valuable. For courses that are more industrial and geological. McCrone Research In Chicago offers many course in quantitative microscopy especially using polarized light microscope. Fo...

You may want to look into the Microscopy courses offered at Woodshole, Mass and Cold Springs Harbor, in New York. These are high level microscopy courses with extensive support from the big four manufacturers including manpower to support workshops and instrumentation. Of course, this give the compa...

Flatness or Plan is a relative concept in microscope objectives. Any objective can be made flat if the FN of the eyepiece is reduced enough. As pointed out, the EF supported up to a FN of 18mm. However it quickly fell off beyond that . Keep in mind, that most modern microscopes offer 20 to 22mm FN a...

The last iteration of the UFM4 used the tube from the DM1000 HC variety. They had a 200mm RFL. So as has been pointed out, the indicated Mag on the objective was off slightly. Usually wasn't a critical concern as no critical measurements were taken on the scope and if it measurements were taken, the...

I can condfirm the tubes used for the Comparison scopes were standard varieties from the Series 10 110 and 410 depending on the years produced. so they use the same 182mm reference focal length. All intermediate optics components are compatible, so if a mag changer from any of those series a 1.25x e...

These are objectives that were used on the UFM 4 Comparison microscope produced in Buffalo. These products came out of the special jobs section of AO. Cat number usually started the the letter K. No B&L involvement and it did carry over to the time of the Leica era. There were many generations of th...

You could look for a Leica DM RXA, The nosepiece is motorized and encoded, the focus control and stage x/y speed is automatically adjusted for the objective in use. Contrast methods are automatically detected and with the right Leica digital camera, meta data from the scope and the camera is capture...

I was review some of the earlier posts in this thread and thought I would update a couple of points. Apochromaut did a very accurate listing of the time line for AO/Reichert corporate migration via MA activites. He referenced on hole, in regards to the Polylite. The first reference I have for the Me...

The fact that DIC is a polarized light technique, it would stand to reason that POL and DIC would be used simultaneously. Combining RL and TL contrast methods are certainly possible. RL Fluorescence with TL DIC or Phase or even simple POL is often done and certainly was one of the advantages of the ...

Keep in mind that the back focal plane was not a big issue because even in the era of finite optics, Reichert upper prisms for DIC had the ability to move the prism vertically relative to the back aperture. Meaning only one prism was used for all magnifications

All Reichert objective were built to a strain free standard so they were conditionally strain free. Those marked suitable for IK were selected as the most strain free and optimized for work in POL applications. . Keep in mind, that strain can be introduced simply be overtightening an objective in th...

The Series 100 was marketed as the parsimonious alternative to the Series 110. As pointed out, it had a different finish, the objective printing was different and if I remember correctly came with 18mm FOV eyepieces rather than 20mm. All in all, back in the day, AO was the market leader in hospital ...

Thanks for jarring my memory, On the Bio platform the 4th mag changer position included the bertrand lens, The 2x and or 2.5x was used on the Met and SC versions. The primary function of the bertrand lens was to view the back aperture of the objective for phase ring alighment.

As I pointed out earlier, the Leica Delta optics are a reference 200mm focal length w/ M25 objective threads. vs 180mm RMS for the Vienna based objectives.(Bio) As Apochronaut pointed out the US based objectives with the US eyepieces for the 45mm Reichert objectives had a different ca from the Vienn...

Lots going on since my last post, I agree scanning the lit will be helpful. Regarding Delta optics. Objective thread size is different from the DMR and the Polyvar and the reference focal length is different as well as lateral color correction in the tube lens. An image could be formed, but would no...

From the 80's onward, the Polyvar that were in the field morphed into the into the Polyvar SC and further iterations used in the Semiconductor business. Leica spun off a separate company that focused solely on the Semi Business including Lithography systems and other tools for fabs. . The Bio and st...

I finally dug into my Reichert catalog to try and find this objective. I recall it in the program for the Polyvar, but can't realize the details. I have reference materials for the Life Science Polyvar, and Polyvar 2 as well as the Polyvar Met, Polylites up to the 88 with literature from 1983 to 199...

Centering keys are used for the annular diaphragm adjustment in phase contrast. Fine tuning of the DF stop could also be used. Otherwise, Fluoro, POL, BF will also function with the open aperture of the turret. Centering the condenser to the Field Diaphragm should be independent of the annular stops...

Any reflected objective of magnification less that 2x using coaxial illumination would need a contrast cap. Essentially a 1/4 lambda plate that is rotated to achieve the best contrast. This is also requires crossed polars in the illumination axis. With out these devices, contrast is seriously compro...

I took a closer look at the DIC slider and I am fairly certain it is for an MeF3/4.

Sorry I haven't been on the forum in a while. Here are a couple of update points relative to the Univar/Polyvar. If i recall correctly, the Univar was introduced about 1973 and the Polyvar approximately 1975. The major difference is the Polyvar required phase specific objectives with annular rings o...

I do have a DIC slider, but I am not sure if it is for the Polyvar or the MeF4

I wonder if you could modify the Aux Lens from the AO Seriese 10/110 abbe condenser to work. There should be bazillion of them out there cheap and would require mostly modification to the mounting point.

Quite the impressive collection of Zetopans spanning a number of iterations. the silver is the latest vintage while the light cream color was done for the exclusive distribution by Hacker prior to AO purchasing Reichert in the 60's. Black is the oldest. I see the silver one has a Hacker label on it,...

Impressive set up. And Clearly the price was/is right. There are two contrast condenser offered in my reference material. 9 99 00 05 Contrast condenser, N.A. =0.98 turret with 6 annular aperture diaphragms (1-6) 3 empty apertures, centering deice, aperture iris diaphragm, with wide field optics, on ...

I have the 1978 version of the Zetopan order guide. This is from the last year of production and availability, as the as the Univar (1974-5ish) and subsequently the Polyvar (1976ish)were being brought The only reference to the UV condenser is for use with the Binolux illuminator for fluorescence and...

The short answer is yes, DIC is possible with the Polyvar. I have two universal condensers with prism and phase rings for the Polyvar. I also have a couple of objective for this microscope. I may also have a Polyvar biological nosepiece. Yes, it only used one objective prism for all magnifications. ...

Is this Polyvar a Bio version or the Met version? I know I have the universal turret condenser for the Bio version with the Phase and DIC prism. I thought I had a stage, but have not located in my storage yet. Are you located in the US?