MicrobeHunter.com Microscopy Forum

You really don't want to remove the excitation filter. Although about inch of glass will prevent the transmission of harmful UV rays, the intensity would overwhelm the retina. In addition, the emission from autofluorence is still wavelength dependent. In otherwords it needs the excitation to generat...

Thanks for the comment and good luck with your investigations.

The Biostar was never offered with Leica branded objectives, although it was infinity corrected so someone may have built a system with objective from an DM IRB. On this point I am positive you are wrong, to the extent that it matters. I never bought an 1820 from the manufacturer, but you frequentl...

Phil, I agree completely.

The Biostar was never offered with Leica branded objectives, although it was infinity corrected so someone may have built a system with objective from an DM IRB. However, it would require RMS/M25 adapter rings to fit the objective into the nosepiece. Of course, it would have been possible to mount L...

The article referenced in association with this objective specifically mentioned multi - photon applications. Terminology associated with Confocal Microscopy. I suspect the low mag is desired because of thick tissue samples and the high NA is beneficial because it will aid in presenting an intensity...

I had some old literature that provided some detailed information. After the specs is my commentary of the applications and image information. 1711 was used with Auramine stain. (436/450/GG475) This was common for TB bacillus that had excitation in the 436nm range and emissions in the in the yellow ...

It seems this objective would lend itself to applications with confocal microscopy. High NA provides better light amplitude combined with higher DOF associated with the lower magnification. Thicker samples could be viewed, while eliminating the noise in the interstial tissue areas.

This is a great discussion when it comes to comparing theoretical vs. practical real world application of the condenser diaphragm adjustment. Most of the major manufacturers have used some form of reference to the objective, in use, on the condenser, giving the user a reference point. It doesn’t mat...

Pete,
Sorry I don’t know the specifics on those objectives. It might be possible to search for the spectral curve on each objective, ideally using the cat number if you happen to have it

As Pete M Indicated, the UVFL objectives are for vertical microscopy, which really is reflected light, not transmitted applications. I don't have the spectral curves for these objectives, but typically a UV reference is good to something in the range of 365nm. This is typical for a good biological r...

Did any of the proposed calculation provide an answer that was comparable?

If you want to know the final magnification, measure the field of view in mm and decide by the FOV of the eyepiece. So if the FOV through microscope is 18mm the total mag is 1x. If the FOV is 1.8mm your total mag is 10x. Etc as long as you know the FOV of the eyepiece, you can determine the total ma...

Keep in mind almost all industrial microscopes are augmented biological scopes with accommodation for illumination path and specimen manipulation. Then rebranded to reflect those differences

I can tell you this is definitely not an adapted Reichert industrial objective from the Vistek Polyvar range. When the Vistek name was implemented as a spin off from Leica, the Vienna factory was already out of the microscope business. The continue to make EM instrumentation, today. The 11767xxx cat...

A couple of additional points of reference relative to this objective. Not only does this objective need a condenser capable of transmitting UV the entire illumination system must also support UV transmission. If this objective was designed for transmitted light applications such as fluorescence, th...

Is the question how to determine the centration of the optical path, or what is the best way to me mechanically position the LED element? If it is the previous, recall there are conjugate planes in the microscope, of which one is the illumination plane. The objective back aperture, aperture diaphrag...

As an addendum to apochronaut’s thread, parfocality on a Reichert Zetopan and later on the Univar/Polyvar scopes was achieved by hand honing the shoulder of the objective. This was specific to the position in the nosepiece and each scope. It was one of the reasons the objective mag position was mark...

with that spectral curve, that is not a halogen lamp. Typically halogen lamps use a filament. The Leitz projectiion scope usually had a XBO lamp depending on vintage. 150W most likely but early systems used a 450XBO. You can't miss it. It is very large as is the power supply. Also that combo often m...

Absolutely correct. I was oversimplifying, Thanks for clarifying.

Generally when you encounter a short coming such as the stage x-y control there is often a corresponding plus. In this case, the stage level coaxial stage control offer a bilateral access so you can use either the left or right hand to operate the stage movement. It might not be a plus for you, but ...

I haven’t seen a two diaphragm condenser for the Zetopan since the 70, As Phil pointed out, the bottom iris functions as a field diaphragm, while the upper iris is the aperture diaphragm. The dark field stop would need to be at that conjugate plane. I don’t think there is a practical way to accompli...

I just took a look at the bid site and a side picture. This system has a unique to Leitz ( Leica) component installed between the lamp house and the microscope stand. It is a variable color filter system that was used to help balance the color between the left and right scopes. As I mentioned earlie...

This particular system, simply known as a comparison microscope, is used in the trace evidence labs for forensic investigations. Typical samples would include hair, fibers, glass and paint chips. To a lesser extent, building materials, minerals and other samples that would be suitable for use on a P...

Phil's description is spot on. I have not found any in my stash in any case.

I used to have a couple of different types that were essentially homemade. I'll see if I can find one in an old tool kit and take a picture. One was wooden, with a soft metal strap, that other was all metal.

Regardless of the microscope model, one factor that should be considered in low mag photomicrography is the parallax that can occur to the conjugate image plane Does the viewing eyepiece have a reticle that can be focused with to the eye? If not, it can be critical to the focus process of the scope ...

You will need an objective wrench in order to get them off. An objective wrench in similar in design to a jar opener, only the scale is brought down to fit into the nosepiece and objective.

If you are trying to take the objectives off the Serise 50/150 or Series 60/160, you may encounter some difficulty. The objectives on those models were tightened into the nosepiece with objective wrench. This prevented students from removing an subsequently damaging an objective. It they were instal...

Which eyepiece do you currently have. The 10x/20 eyepiece for the DMLB will accept a reticle. There are a number of them available depending on what and how you want to measure. The reticle is placed at the conjugate image plane of the eyepiece. It will be in focus with the sample. The only need for...