Search found 9 matches

by Bradscopegems
Tue Nov 03, 2020 1:52 am
Forum: Microscopes and optics
Topic: Field of View - Zeiss Universal vs. Leitz Orthoplan
Replies: 15
Views: 15356

Re: Field of View - Zeiss Universal vs. Leitz Orthoplan

Am I alone in finding that there is a downside to wide-field eyepieces? The reason is that the observer's head must be held more steady than with low FOV eyepieces such as the classic Zeiss 8x eyepieces with FOV 18 or 20mm. The distance of the eye from the top of the eyepiece must be kept almost con...
by Bradscopegems
Fri May 31, 2019 10:30 am
Forum: Camera systems and imaging
Topic: Microscope Camera Recomendatio,
Replies: 19
Views: 11483

Re: Microscope Camera Recomendatio,

Dear Hobbyst46, You are right. I was using white light, so taking the wavelength as 500nm, the resolution would have been 0.2um, not 0.15. I remember also that I was using a 60x objective, so the resolution in image space would be 12 um, or 5 pixel widths: just above Nyquist. The Nikon Optiphot with...
by Bradscopegems
Thu May 30, 2019 11:07 pm
Forum: Camera systems and imaging
Topic: Microscope Camera Recomendatio,
Replies: 19
Views: 11483

Re: Microscope Camera Recomendatio,

Dear Hobbyst46, You are right to worry about chromatic aberrations. To minimise this danger, I place the 183M so that the camera chip is in the intermediate image plane, and avoid the presence of any eyepiece, mag changer or other glassware. When I use a 100x objective, N.A. 1.4 oil immersion, the r...
by Bradscopegems
Thu May 30, 2019 9:09 pm
Forum: Camera systems and imaging
Topic: Microscope Camera Recomendatio,
Replies: 19
Views: 11483

Re: Microscope Camera Recomendatio,

Pleurosigma detail.jpg Pleurosigma reduced.jpeg Dear 75RR, Will do, though the very small digital size limit of the images that can be accepted in this forum will take some time to circumvent: I will have to show whole images and also enlarged details..... Here is a taster: an image of a diatom str...
by Bradscopegems
Thu May 30, 2019 8:38 pm
Forum: Camera systems and imaging
Topic: Microscope Camera Recomendatio,
Replies: 19
Views: 11483

Re: Microscope Camera Recomendatio,

Dear Hobbyst46, As you say, high sensitivity is essential for most fluorescence microscopy, which is the most valuable form for biomedical research at present because it allows chemical specificity in staining, but there are other reasons for needing high sensitivity. Many events in the microscopic ...
by Bradscopegems
Thu May 30, 2019 4:51 pm
Forum: Camera systems and imaging
Topic: Microscope Camera Recomendatio,
Replies: 19
Views: 11483

Re: Microscope Camera Recomendatio,

chipsizes.jpg Altair 183M on Optiphot.jpg I have no commercial connection with them, but strongly recommend that anyone looking for a microscope camera should investigate the 183M and 183C monochrome and colour cameras sold by Altair Astro. For high sensitivity and low noise the cameras sold to wel...
by Bradscopegems
Tue May 28, 2019 3:36 pm
Forum: Microscopes and optics
Topic: Does anyone have instructions for the Zeiss Linnik microscope?
Replies: 7
Views: 4671

Re: Does anyone have instructions for the Zeiss Linnik microscope?

Linnik optical diagram (Kingslake).jpg Dear Microb and MichaelG, Thank you for your suggestions. I have sent an email to the dutch group who modernized a Linnik very similar to mine and will let you know if they respond. I am attaching here an optical diagram from Applied Optics and Optical Enginee...
by Bradscopegems
Tue May 28, 2019 12:03 am
Forum: Microscopes and optics
Topic: Does anyone have instructions for the Zeiss Linnik microscope?
Replies: 7
Views: 4671

Re: Does anyone have instructions for the Zeiss Linnik microscope?

US06721094.jpg Thanks for your reply. Unfortunately, the Wiki image is not helpful, because it shows an interferometer modified for other purposes so that it is no longer recognizable as a Linnik. I am attaching a better basic diagram from a US patent. The Zeiss instrument contains a lot of extra o...
by Bradscopegems
Sat May 25, 2019 11:56 pm
Forum: Microscopes and optics
Topic: Does anyone have instructions for the Zeiss Linnik microscope?
Replies: 7
Views: 4671

Does anyone have instructions for the Zeiss Linnik microscope?

Does anyone know how to set up the Carl Zeiss West germany version of the Linnik microscope, which is a massive cube of metal designed for measuring the contours of reflective specimens such as metal surfaces to sub-wavelength accuracy. I bought one of these on eBay some years ago and have just star...