Search found 406 matches
- Tue Mar 01, 2022 6:14 pm
- Forum: Camera systems and imaging
- Topic: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
- Replies: 23
- Views: 5957
Re: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
Depth of field is a serious issue: requiring an optimal set-up: starting with coverglasses 1.5 of exact 0.170 mm thickness (the expensive Zeiss CG come to mind here), pond samples require a VERY thin fluid layer (so no debris in your sample). Alternatively I often use 1.0 coverglasses to compensate ...
- Tue Mar 01, 2022 6:00 pm
- Forum: Camera systems and imaging
- Topic: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
- Replies: 23
- Views: 5957
Re: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
This (non recent) website contains a lot of photo micrography info including an article on achieving parfocality . https://krebsmicro.com/ Alternative source is Alan Woods Olympus website and his article on photography using a BH2 http://www.alanwood.net/downloads/olympus-bh2-microscope-attach-digit...
- Tue Mar 01, 2022 3:40 pm
- Forum: Beginner's corner
- Topic: Old vs new stereo microscopes
- Replies: 7
- Views: 2578
Re: Old vs new stereo microscopes
Point to consider: 2nd hand scopes used for electronic repairs often suffer from fogged lenses due to the solder fumes.
- Sun Feb 27, 2022 7:43 am
- Forum: Microscopes and optics
- Topic: Cover slip thickness
- Replies: 4
- Views: 1546
Re: Cover slip thickness
Peter, Yes coverglass thickness matters, definitely for objectives with an NA (numerical aperture) of 0.6 and higher (for common achromat objectives this means 40* and above). See my answer to your previous “objective” question. Allow me an observation: this forum is willing to help, a substantial s...
- Sat Feb 26, 2022 10:42 am
- Forum: Beginner's corner
- Topic: Objective Lenses
- Replies: 3
- Views: 1499
Re: Objective Lenses
The common way of mounting the objectives is from low to high power in clockwise direction. What objectives to mount is influenced by personal preference and usage area. E.G when viewing botanic cuts i use 4-10-20-40 (4* is my most used one) when viewing pond samples 4-10-20-40 (20* is my most used ...
- Wed Feb 23, 2022 12:54 pm
- Forum: Beginner's corner
- Topic: Olympus BH-2 objectives
- Replies: 10
- Views: 3789
Re: Olympus BH-2 objectives
Mycogirl: you have a PM.
- Tue Feb 22, 2022 6:55 pm
- Forum: Beginner's corner
- Topic: Olympus BH-2 objectives
- Replies: 10
- Views: 3789
Re: Olympus BH-2 objectives
I have both BH2 and CH2 scopes. The Olympus CH2 serie has 20 Watt halogen or 30 Watt filament illumination, the 20 Watt has enough power for all bright field and phase options except 100* phase contrast (it is relatively simple to add a LED option if you insist: main reason to do this is constant co...
- Fri Feb 18, 2022 9:31 pm
- Forum: Pictures and Videos
- Topic: Galveston Bay Plankton
- Replies: 7
- Views: 1934
Re: Galveston Bay Plankton
Nice, hope to explore some North Sea samples coming weeks myself.
Please keep them coming.
Please keep them coming.
- Mon Feb 07, 2022 1:17 am
- Forum: Miscellaneous
- Topic: transport of a heavy microscope - how to handle it?
- Replies: 18
- Views: 6416
Re: transport of a heavy microscope - how to handle it?
I stopped trying to transport the BH2 and added a CH2 to my artillery, really appreciate the little one. It is now equipped with a phase condenser and LED (the later saves additional weight), it is transported (I’m on public transport) in a small flight case with some extra padding. Approx 6-7 kg in...
- Sat Feb 05, 2022 7:58 pm
- Forum: Microscopes and optics
- Topic: The common Olympus 1.25 condenser...tips?
- Replies: 5
- Views: 1720
- Sat Feb 05, 2022 5:20 pm
- Forum: Microscopes and optics
- Topic: The common Olympus 1.25 condenser...tips?
- Replies: 5
- Views: 1720
Re: The common Olympus 1.25 condenser...tips?
And oiling the condenser-slide will usually cause a bit of a mess…. Using a thicker oil helps, I often use distilled water or glycerine instead: much easier to clean and it helps enough to increase the combined condenser-objective NA. For max coverage of the objective NA oiling the condenser is a mu...
- Fri Feb 04, 2022 8:45 pm
- Forum: Microscopes and optics
- Topic: Olympus 20x Splan vs 20X Splan APO
- Replies: 6
- Views: 2327
Re: Olympus 20x Splan vs 20X Splan APO
Can confirm the depth of field concern (but in my book: tolerable at 20*) and the required Zeiss 0.17 coverglasses of precise thickness, but still .. love the image quality. At 40* I often use the plain 40* achromat for pond samples (ciliates etc) to gain a little in the depth of field / focus area....
- Fri Feb 04, 2022 6:38 pm
- Forum: Microscopes and optics
- Topic: Olympus 20x Splan vs 20X Splan APO
- Replies: 6
- Views: 2327
Re: Olympus 20x Splan vs 20X Splan APO
Terry, I have both and love the Apo, somehow the “micro-contrast” is preferable to my eyes. Consider it one of the best objectives from the LB era, I even bought a second one for my field CH2 scope (overkill but nice). A color corrected (AAC or SC) condenser helps to get the most out of it. Pond dig...
- Thu Feb 03, 2022 3:20 pm
- Forum: Pictures and Videos
- Topic: Planktonic Diatoms
- Replies: 16
- Views: 4393
- Tue Feb 01, 2022 8:54 am
- Forum: Pictures and Videos
- Topic: Planktonic Diatoms
- Replies: 16
- Views: 4393
Re: Planktonic Diatoms
Nice ones Kurt,
As a NEWBY on seawater creatures: please recommend some literature guidance to START identifying the species.
Thank you,
deBult
As a NEWBY on seawater creatures: please recommend some literature guidance to START identifying the species.
Thank you,
deBult
- Tue Feb 01, 2022 8:50 am
- Forum: Introduce yourself
- Topic: Astronomer Considering Microscopy
- Replies: 54
- Views: 16685
- Mon Jan 17, 2022 6:22 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Wanted: Olympus BH2-BHS (or similar BH2, etc.)
- Replies: 8
- Views: 3197
- Sun Jan 16, 2022 8:15 pm
- Forum: Illumination Techniques
- Topic: Problem with choosing the patch stop size for the 40x lens
- Replies: 9
- Views: 3734
- Sun Jan 16, 2022 8:05 pm
- Forum: Illumination Techniques
- Topic: Problem with choosing the patch stop size for the 40x lens
- Replies: 9
- Views: 3734
Re: Problem with choosing the patch stop size for the 40x lens
Can I use immersion oil (40x) in my lenses, even though they don't have "oil" written on them? Can immersion oil get into / between the lens of the objective? How do you clean your lenses of oil? You will ruin your 40* PlanApo when using oil between lens and sample/coverglass. Only use oil on objec...
- Thu Jan 13, 2022 8:24 pm
- Forum: My microscope
- Topic: Old Leitz HM-LUX (Info ? maintenance ? upgrade ?)
- Replies: 3
- Views: 1837
Re: Old Leitz HM-LUX (Info ? maintenance ? upgrade ?)
Zeiss cleaning guidelines
https://www.zeiss.com/microscopy/int/so ... scope.html
Leitz manuals etc
http://microscope.database.free.fr/Welcome.html
https://www.zeiss.com/microscopy/int/so ... scope.html
Leitz manuals etc
http://microscope.database.free.fr/Welcome.html
- Mon Jan 10, 2022 6:35 pm
- Forum: Home-made microscope adaptations
- Topic: Led Retrofit
- Replies: 8
- Views: 2810
Re: Led Retrofit
My dark ground/FIELD success rate at the CH2 is using the Olympus CH2-PCD condenser at 40* (or should I say NA max 0.65)
Note 10 Watt LED on an Olympus CHx may be a danger to your eyes when used at full power.
Note 10 Watt LED on an Olympus CHx may be a danger to your eyes when used at full power.
- Mon Jan 10, 2022 5:09 pm
- Forum: Home-made microscope adaptations
- Topic: Led Retrofit
- Replies: 8
- Views: 2810
Re: Led Retrofit
My CH2 LED is set at max 0.7 Watt, plenty of light even for dark ground and phase.
Ahh my error it is using 0.7 A not Watt.
Ahh my error it is using 0.7 A not Watt.
- Tue Jan 04, 2022 5:20 am
- Forum: Microscopes and optics
- Topic: What was this weird Leitz system used for?
- Replies: 7
- Views: 2452
Re: What was this weird Leitz system used for?
Typically used for sample comparison in criminal investigations.
(E.g. for drowned victims: identical diatom sample in lungs and waterway).
(E.g. for drowned victims: identical diatom sample in lungs and waterway).
- Wed Dec 29, 2021 10:57 am
- Forum: Microscopes and optics
- Topic: Olympus NC Splan APO 60x spring dissasembly
- Replies: 14
- Views: 3928
Re: Olympus NC Splan APO 60x spring dissasembly
Try a blood SMEAR not a drop of blood Technique: UPDATE previous link used a cover glass, now a true smear https://okean.rs/preparation-of-blood-smear/#:~:text=The%20most%20common%20technique%20of%20blood%20smear%20preparation,midline%20at%20the%20end%20of%20a%20glass%20slide. or https://medicallabt...
- Wed Dec 29, 2021 9:38 am
- Forum: Microscopes and optics
- Topic: Olympus NC Splan APO 60x spring dissasembly
- Replies: 14
- Views: 3928
Re: Olympus NC Splan APO 60x spring dissasembly
The coverslip is part of the optical correction mechanism: so if you use it without: this causes the "softness" you observe. In general objectives 40* NA 0.60 and higher require coverglasses of EXCACT 0.17 mm tickness, most coverglasses range from 0.13-0.18, even the ones used on commercial slides (...
- Tue Dec 28, 2021 7:42 pm
- Forum: Microscopy accessories
- Topic: Advice On Buying Parts On eBay
- Replies: 23
- Views: 10665
Re: Advice On Buying Parts On eBay
Double check postage cost as well, sometimes the object is cheaply priced and postage ridiculously high.
- Wed Dec 22, 2021 5:51 pm
- Forum: Microscopes and optics
- Topic: Disassembling Objective lenses
- Replies: 7
- Views: 3380
Re: Disassembling Objective lenses
There is a higher viscosity (thick) type oil for inverted scopes.
- Fri Dec 10, 2021 5:43 pm
- Forum: Microscopes and optics
- Topic: Anti-reflection coating durability
- Replies: 8
- Views: 2259
Re: Anti-reflection coating durability
I used a Zeiss lens wipe on the internal bino head prism of an Olympus CH2, a gentle wipe was enough to damage the coating, never had an issue with Olympus objectives of the same era.
- Fri Dec 10, 2021 12:36 am
- Forum: Beginner's corner
- Topic: Oil Recommendations
- Replies: 3
- Views: 1319
Re: Oil Recommendations
Marked oil and/or HI.
- Thu Dec 02, 2021 4:59 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: looking for a non functionnal Olympus SZH body
- Replies: 17
- Views: 5382
Re: looking for a non functionnal Olympus SZH body
I you consider them “total loss” try polishing them with Brasso copper polish and an old cotton Tx-shirt.
Will remove any coating so a bit loss of contrast, but reasonable chance you are able to get the thing working again.
Will remove any coating so a bit loss of contrast, but reasonable chance you are able to get the thing working again.