Search found 137 matches

by deBult
Sat Nov 14, 2020 3:29 pm
Forum: Microscopes and optics
Topic: Broken Microscope
Replies: 20
Views: 4483

Re: Broken Microscope

Often younger children have problems matching left and right eyepiece view. So mono is OK.
by deBult
Sat Nov 14, 2020 4:39 am
Forum: Microscopy accessories
Topic: Question: What is the blue filter for?
Replies: 4
Views: 483

Re: Question: What is the blue filter for?

Net result of using the bleu filter is the background of your viewed specimen will get a more “daylight” character: it will move from “yellowish” to “blue sky”.
by deBult
Fri Nov 06, 2020 1:06 pm
Forum: Beginner's corner
Topic: Using glass wipes to clean objectives?
Replies: 12
Views: 429

Re: Using glass wipes to clean objectives?

Chamois leather
Yes thank you.
by deBult
Fri Nov 06, 2020 5:57 am
Forum: Beginner's corner
Topic: Using glass wipes to clean objectives?
Replies: 12
Views: 429

Re: Using glass wipes to clean objectives?

For oil objectives older microscopists often use a drop of saliva on a soft leather (the one you use when cleaning/drying windows: can’t remember the proper word in English).

Take proper precautions: immersion oils may be related to cancer and older oils may contain LOTS of PCB’s.
by deBult
Fri Nov 06, 2020 5:54 am
Forum: Beginner's corner
Topic: Using glass wipes to clean objectives?
Replies: 12
Views: 429

Re: Using glass wipes to clean objectives?

Using organic solvents or alcohol can be devastating to VERY old objectives [1840-1920] and some more recent ones as well. Proper distilled water (distilled twice when you are a perfectionist) with 1 tiny drop of fairy dishwashing liquid per 50-100 ml will do the job for most. Don’t use Kim wipes or...
by deBult
Fri Nov 06, 2020 5:06 am
Forum: Beginner's corner
Topic: Starting Photomicrography - BH2 objectives
Replies: 7
Views: 468

Re: Starting Photomicrography - BH2 objectives

Evey other scope would have a similar issue: you will need a matching projecting eyepiece - camerasensor combination. (And yes even for the alternative using an intermediate lens). So aim for the NFK 2.5 photo eyepiece, try the Sony you already own first. The field of view for the camera using the N...
by deBult
Wed Nov 04, 2020 6:42 pm
Forum: Microscopes and optics
Topic: Combining short and long barrel objectives.
Replies: 10
Views: 453

Re: Combining short and long barrel objectives.

Sooner or sooner (not later) you will crash the 20* into the slide (don’t ask how I known).

Bets, deBult.
by deBult
Mon Oct 26, 2020 2:23 am
Forum: Beginner's corner
Topic: Olympus Vanox
Replies: 9
Views: 556

Re: Olympus Vanox

by deBult
Sun Oct 25, 2020 12:28 pm
Forum: Microscopes and optics
Topic: Hidden focus tension adjustment?
Replies: 8
Views: 422

Re: Hidden focus tension adjustment?

Same issue on Olympus CHX and BHx series: do NOT use this “counter-twist” option as it will ruin the gears of your scope.

This option was in use at older mono ocular scopes.

Thus RTF!
by deBult
Sat Oct 24, 2020 5:27 am
Forum: Beginner's corner
Topic: 80% Sure on the purchase of my first microscope
Replies: 5
Views: 409

Re: 80% Sure on the purchase of my first microscope

Nice scope (I own one similar). This particular phase condenser (with 20* setting) has no dark field stop, but the 100* phase stop does a really nice DF up till the 20* objective. A DF cube is of no use in this setup. The loss in contrast - when using phase objectives in normal bright field - is not...
by deBult
Thu Oct 22, 2020 1:11 am
Forum: Specimens, samples and slides
Topic: How to prepare and preserve tardigrades on a slide
Replies: 14
Views: 1174

Re: How to prepare and preserve tardigrades on a slide

Tnx: this link works (never received any answer when requesting the paper).
by deBult
Tue Oct 20, 2020 4:54 am
Forum: My microscope
Topic: My not-Nikon, not S mystery microscope
Replies: 7
Views: 639

Re: My not-Nikon, not S mystery microscope

Nippon Kogaku was rhe pre WW2 name used on Nkon products. From Wikipedia: Founded on July 25, 1917 as Nippon Kōgaku Kōgyō Kabushikigaisha (日本光学工業株式会社 "Japan Optical Industries Co., Ltd."), the company was renamed to Nikon Corporation, after its cameras, in 1988. Nikon is a member of the Mitsubishi g...
by deBult
Sun Oct 18, 2020 4:48 am
Forum: Microscopes and optics
Topic: poor sharpness + contrast using 40X on self prepared slides
Replies: 5
Views: 423

Re: poor sharpness + contrast using 40X on self prepared slides

Any progress on your 40* sharpness concern? Slides build with Xylene based mountants in general have longer shelf life plus the breaking index is higher, the latter often useful with objects with high transparency. Xylene is a health hazard and requires proper ventilation at minimum. I have water mo...
by deBult
Wed Oct 14, 2020 12:43 pm
Forum: Specimens, samples and slides
Topic: How to prepare and preserve tardigrades on a slide
Replies: 14
Views: 1174

Re: How to prepare and preserve tardigrades on a slide

Tnx, requested the paper.

Thermal inactivation of 30 mins at 60 centigrade would suffice.... and they send them to the moon and expect them to reincarnate when returned to earth???
by deBult
Tue Oct 13, 2020 7:42 pm
Forum: Specimens, samples and slides
Topic: How to prepare and preserve tardigrades on a slide
Replies: 14
Views: 1174

Re: How to prepare and preserve tardigrades on a slide

Would appreciate some guidance as well.
by deBult
Thu Oct 08, 2020 9:26 pm
Forum: Introduce yourself
Topic: Hello from Buenos Aires
Replies: 22
Views: 1037

Re: Hello from Buenos Aires

I have been examining the condenser issue quite a lot. I can center it with the 10 x objective (by sight, I have not the so called telescope eyepiece), but it doesn't seem to keep that very same position when going the next objective.
This is a common issue even when using an older research scope.
by deBult
Sun Oct 04, 2020 2:19 pm
Forum: Introduce yourself
Topic: Hello from Buenos Aires
Replies: 22
Views: 1037

Re: Hello from Buenos Aires

Ha a “Franken-scope”: nice. Note: The 40* objective is very sensitive to correct coverglass thickness, so maybe try another brand? Best, deBult Indeed! :lol: I'm not sure what you mean by the coverglass thickness correction... I guess it's an issue of the focal point? How is this related to the fie...
by deBult
Sat Oct 03, 2020 9:14 pm
Forum: Introduce yourself
Topic: Hello from Buenos Aires
Replies: 22
Views: 1037

Re: Hello from Buenos Aires

Ha a “Franken-scope”: nice.

Note: The 40* objective is very sensitive to correct coverglass thickness, so maybe try another brand?

Best, deBult
by deBult
Sat Oct 03, 2020 9:09 pm
Forum: Introduce yourself
Topic: Hello from Buenos Aires
Replies: 22
Views: 1037

Re: Hello from Buenos Aires

Ha Javier,

Welcome, please share some pictures of the scope you are using.

Best, deBult
by deBult
Fri Sep 25, 2020 8:00 pm
Forum: Specimens, samples and slides
Topic: How to remove fungus from 100 year old diatom slides
Replies: 3
Views: 526

Re: How to remove fungus from 100 year old diatom slides

Tnx for the guidance.

Had already replaced the original box.
Will try a woman's nail UV clearing unit and see how much the labels suffer.
by deBult
Tue Sep 22, 2020 6:43 pm
Forum: Specimens, samples and slides
Topic: How to remove fungus from 100 year old diatom slides
Replies: 3
Views: 526

How to remove fungus from 100 year old diatom slides

May I ask your combined knowledge for assistance please. I bumped into a box of 85 old Diatom slides. Age unknown but the glass of the slides looks and 'feels' very old (typical streaks like you see in windows panes begin 1910). Descriptions in Chinese ink or in pencil. All slides show different Dia...
by deBult
Sun Sep 20, 2020 6:38 am
Forum: Identification help
Topic: Is this a tardigrade?
Replies: 19
Views: 2497

Re: Is this a tardigrade?

Sampling the next day AFTER a good shower of rain helps: gives them time to become active again.

For dry moss I soak in rain water for 24 Hrs.

Best, deBult
by deBult
Sun Aug 30, 2020 8:53 am
Forum: Beginner's corner
Topic: Microscope hunting
Replies: 14
Views: 1952

Re: Microscope hunting

I have never been able to score a 100% intact scope on eBay: all needed some work: some substantial (the price was no good indicator on the rework required). The last BH2 bought required a full CLA, new focus knobs, 2 new stage racks (cracked), a new condenser rack (broken). Individual objectives “o...
by deBult
Mon Aug 24, 2020 7:26 pm
Forum: Microscopy accessories
Topic: condenser insert set
Replies: 12
Views: 1620

Re: condenser insert set

iconoclastica wrote:
Sun Aug 23, 2020 12:24 pm
deBult wrote:
Sun Aug 23, 2020 11:29 am
These are INSERTS into the condenser and close to the diaphragm. I’m pretty please with my set.
What type of condenser are you using? On my 0.90 achromat the diaphragm resides c. 30mm above the bottom plate.
Olympus BH2 with standard Abbe condenser or Phase condenser.
by deBult
Sun Aug 23, 2020 11:29 am
Forum: Microscopy accessories
Topic: condenser insert set
Replies: 12
Views: 1620

Re: condenser insert set

These are INSERTS into the condenser and close to the diaphragm. I’m pretty please with my set.
by deBult
Sat Aug 22, 2020 5:30 pm
Forum: Illumination Techniques
Topic: Phase Contrast or Brightfield if all things are equal?
Replies: 27
Views: 2323

Re: Phase Contrast or Brightfield if all things are equal?

Read Apochromat previous post please.

Go for the swap even to a none phase 100* objective.
This one is only (but very) usefull in obsevering (blood) smears without coverglas: a very specialist and niche field of microscopy.
by deBult
Thu Aug 20, 2020 10:13 am
Forum: Illumination Techniques
Topic: Phase Contrast or Brightfield if all things are equal?
Replies: 27
Views: 2323

Re: Phase Contrast or Brightfield if all things are equal?

The external transformer listed worries me: to my understanding the BHS does not need one for brightfield and phase (I’m a BHTU user). This may indicate a failure in the base normally supplying the power.

http://www.alanwood.net/downloads/olymp ... manual.pdf
by deBult
Wed Aug 19, 2020 7:32 pm
Forum: Illumination Techniques
Topic: Phase Contrast or Brightfield if all things are equal?
Replies: 27
Views: 2323

Re: Phase Contrast or Brightfield if all things are equal?

There is a slight reduction in contrast and resolution when using Olympus phase objectives for bright field, you wil only notice the difference when comparing both on the same scope. The observation “ upgrading a brightfield microscope to phase is much more expensive than buying a set of brightfield...