Search found 26 matches

by GrbovicMicroscopy
Wed Nov 06, 2019 7:21 pm
Forum: Beginner's corner
Topic: Lugols iodine
Replies: 2
Views: 132

Re: Lugols iodine

Thank you
by GrbovicMicroscopy
Tue Nov 05, 2019 7:02 pm
Forum: Beginner's corner
Topic: Lugols iodine
Replies: 2
Views: 132

Lugols iodine

Hi, anyone tried staining with Lugol iodine solution? What does it stain? are there any protocols? I acquired some recently.
by GrbovicMicroscopy
Sat Nov 02, 2019 9:38 am
Forum: Important announcements
Topic: New Ammo Directory
Replies: 6
Views: 280

Re: New Ammo Directory

I would order 400 blank slides in 7.62x39 caliber with frosted ends and ground edges! And cover slips in 0.17mm same volume!
by GrbovicMicroscopy
Sat Sep 21, 2019 8:45 am
Forum: Specimens, samples and slides
Topic: Snakeskin specimen
Replies: 13
Views: 1711

Re: Snakeskin specimen

Its a shed, if you by leather mean a piece of processed snakeskin. ... I couldn't think of a better term than 'leather' MichaelG. I understand what you wanted to say, thank you for the article on the process and Bob for the fish scale protocol! 8-) I should have time after work today, so hopefully ...
by GrbovicMicroscopy
Sat Sep 21, 2019 8:21 am
Forum: Specimens, samples and slides
Topic: Snakeskin specimen
Replies: 13
Views: 1711

Re: Snakeskin specimen

MichaelG. wrote:
Sat Sep 21, 2019 7:56 am
GrbovicMicroscopy wrote:
Sat Sep 21, 2019 7:18 am
... Will keep you posted on this if anyone is interested.
Edit: is it a 'shed' or a piece of 'leather' ?
Its a shed, if you by leather mean a piece of processed snakeskin.
by GrbovicMicroscopy
Sat Sep 21, 2019 8:18 am
Forum: Specimens, samples and slides
Topic: Snakeskin specimen
Replies: 13
Views: 1711

Re: Snakeskin specimen

I thought of placing the specimen in isopropyl alcohol directly. Guess that would not do the skin good so i will follow the fish scale technique. As i have enough of the specimen i will try to mount it in Entellan, canada balsam and glycerin jelly. The glycerin jelly will be a challang as it require...
by GrbovicMicroscopy
Sat Sep 21, 2019 7:18 am
Forum: Specimens, samples and slides
Topic: Snakeskin specimen
Replies: 13
Views: 1711

Snakeskin specimen

I have recently acuired a snakeskin specimen, but could not find any information on how to process it for permanent slide making.. Has anyone made a snakeskin slide before? I will try different chemical to soften it a bit as it is a bit older. Will keep you posted on this if anyone is interested.
by GrbovicMicroscopy
Sun Sep 01, 2019 3:12 pm
Forum: Specimens, samples and slides
Topic: Embeding problem, glycerine jelly update and stain sensitivety.
Replies: 2
Views: 448

Embeding problem, glycerine jelly update and stain sensitivety.

Hello So i tried to embed the stem of Petroselinum crispum in paraffin wax, it did not work as the wax did not penetrate the cells so the stem shrunk after a while. I did fixate it in achohol then embeded it. What do i need to soak (fix) the specimen in to get a nice embedment i would like to keep t...
by GrbovicMicroscopy
Tue Aug 27, 2019 7:51 pm
Forum: Specimens, samples and slides
Topic: Glycerin jelly permanent mounting
Replies: 8
Views: 1037

Re: Glycerin jelly permanent mounting

MicroBob wrote:Look at page 30 of this document: https://www.berliner-mikroskopische-ges ... skopie.pdf

As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.

Bob
Thank you Bob very helpful as ever!
by GrbovicMicroscopy
Tue Aug 27, 2019 7:49 pm
Forum: Specimens, samples and slides
Topic: Glycerin jelly permanent mounting
Replies: 8
Views: 1037

Re: Glycerin jelly permanent mounting

I have had some glycerin jelly I made sitting in my lab frig for years now. Very little discoloration. Although I may see some slight signs of microbial growth. LOL Never got around to using it. I was trying a different preservative which has worked incredibly. Which one did you use, if i may ask?
by GrbovicMicroscopy
Tue Aug 27, 2019 7:48 pm
Forum: Specimens, samples and slides
Topic: Glycerin jelly permanent mounting
Replies: 8
Views: 1037

Re: Glycerin jelly permanent mounting

The method is to include a very small amount of 'disinfectant' in the formula, I used 'Lysterine' with very good results indeed when I was experimenting with glycerin jelly mounts. Here's a link to a post re glycerin jelly.. It is your post that inspired me to try it. The thing is i dont know how t...
by GrbovicMicroscopy
Tue Aug 27, 2019 5:17 pm
Forum: Specimens, samples and slides
Topic: Glycerin jelly permanent mounting
Replies: 8
Views: 1037

Glycerin jelly permanent mounting

Hi everyone I made some glycerin jelly today for making permanent slides. What is the procedure for the specimen if it is to be mounted in glycerin jelly? And how do i really mount it in the jelly? Do i heat it until it is liquid and pour some ok the slide? Very interested in glycerin jelly mounting...
by GrbovicMicroscopy
Wed Aug 21, 2019 10:40 am
Forum: Introduce yourself
Topic: Hello everyone!
Replies: 4
Views: 2453

Re: Hello everyone!

Hello! Tell us something about you, your equipment and interests :D
by GrbovicMicroscopy
Wed Aug 14, 2019 9:33 pm
Forum: My microscope
Topic: Information about a microscope
Replies: 12
Views: 1289

Re: Information about a microscope

How small do you need the instrument to be for your purposes? I have several smaller multi objective microscopes I collected over the years because of their exceptional optical quality , with an eye to using them as field scopes. My requirements kept changing over time and I now am in the latter st...
by GrbovicMicroscopy
Tue Aug 13, 2019 7:06 pm
Forum: My microscope
Topic: Information about a microscope
Replies: 12
Views: 1289

Re: Information about a microscope

If you wish to invest in a new binocular field microscope, designed by an expert microscopist specifically for field use, (not inexpensive though), here is a link to the Goren microscope: https://www.mrclab.com/productsList.aspx?PcatID=1239 Thank you but that is out of my price range for sure right...
by GrbovicMicroscopy
Tue Aug 13, 2019 4:03 pm
Forum: My microscope
Topic: Information about a microscope
Replies: 12
Views: 1289

Re: Information about a microscope

This is a type of measuring microscope. It is used mainly to be set upon an opaque object in order to examine some details or measure some details by use of an included reticle. Usually some sort of illumination source needs to be shone against the built in white semi-circular reflector, at the bot...
by GrbovicMicroscopy
Tue Aug 13, 2019 2:51 pm
Forum: My microscope
Topic: Information about a microscope
Replies: 12
Views: 1289

Information about a microscope

Hello everyone. I am looking for a small mobile microscope to take with me on vacation. Browsing through a ebay like site in my country i managed to find this microscope https://m.kupujemprodajem.com/Foto/Objektivi-sociva-i-durbini/Meopta-Praha-mobilni-mini-mikroskop-80752469-oglas.htm#mhs0-15657070...
by GrbovicMicroscopy
Sat Jul 20, 2019 9:55 am
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

@GrbovicMicroscopy About diatom cleaning and mounting, there are truely instructive and real-life exemplary posts by rnabholz (with contributions from others)- http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=10&t=3036&p=58300&hilit=pleurax#p58300 and by Charles (whose knowledgable and...
by GrbovicMicroscopy
Sat Jul 20, 2019 9:51 am
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Can i ask how long have you been interested in this hobby? from teen age on but I soon got stuck as I didn't have access to information and materials. Then in the early 2000s I found the german microscopy forum and the "Mikrofibel", a very good compilation of information on microscopes and micro te...
by GrbovicMicroscopy
Fri Jul 19, 2019 8:36 am
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Hi Petar, Radiolaria mounting in LOCA: http://www.mikrohamburg.de/Programm/Protokoll_20181117.pdf Diatom cleaning and mounting: http://www.mikrohamburg.de/Programm/Protokoll_20170917.pdf Diatom arranging: http://www.mikrohamburg.de/Programm/Protokoll_20170218.pdf Plant sectioning in detail (by Klau...
by GrbovicMicroscopy
Fri Jul 19, 2019 4:47 am
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Here in Germany Euparal is the preferred mounting medium for plant sections. It is tolerant to slight amounts of water, gives beautiful and long lasting slides and is easy to use. Our colleagues in Bonn do very impressive plant sectioning, always aimed at the amateur with his limited means: http://...
by GrbovicMicroscopy
Thu Jul 18, 2019 5:37 pm
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Hi Petar, welcome to the forum! When you can get along withou a binocular tube you gain a much wider range of nice microscopes to choos from, liky your two. The straight tube of a horse shoe microscope degrades the image in fact less than an inclined or trinocular tube does. Would you like to tell ...
by GrbovicMicroscopy
Thu Jul 18, 2019 5:02 pm
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Welcome. True "rust" is iron oxides and carbonate, formed by corrosion of iron-containing metal surfaces. If found on the stage, I would suspect that the scope has been exposed to solutions of common salt (NaCl) or other chlorides. Superficial rust can be removed by covering with dilute phosphoric ...
by GrbovicMicroscopy
Thu Jul 18, 2019 4:54 pm
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Re: Hello from Serbia

Hi GrbovicMicroscopy, welcome Nice to see those "classic" microscopes being used. Do you know their approximate year of manufacture? The PZO microscope i really do not know the year, as for the Reichert i found one picture online that said that my microscope is the 1946 model... But i suspect the s...
by GrbovicMicroscopy
Thu Jul 18, 2019 7:32 am
Forum: Introduce yourself
Topic: Hello from Serbia
Replies: 16
Views: 2151

Hello from Serbia

My name is Petar Grbovic Love the vintage monocular microscopes and use them strictly. I am a student of food technology and work in a biochemical lab as a technician. My microscopes are a PZO Poland(bought second hand 100€) microscope and a Reichert Austria (bought second hand 40€) Interesting thin...