MicrobeHunter.com Microscopy Forum

Ascorbic acid under polar illumination. Slides produced both with open evaporation of a saturated solution, and heat melted under a cover slip. Heat needs to be applied gradually when melting powdered ascorbic acid as it quickly decomposes if applied too rapidly. Crystals can take up to a week to fu...

75RR the likely origin of this is your monitor's internal power supply. In order to achieve the voltages and switching speed needed the internal PSU has switching transformers which convert both voltage and frequency. If not properly filtered or the power supply is failing, high voltage, high frequ...

75RR the likely origin of this is your monitor's internal power supply. In order to achieve the voltages and switching speed needed the internal PSU has switching transformers which convert both voltage and frequency. If not properly filtered or the power supply is failing, high voltage, high freque...

Menthol is a bit slower to form crystals than others I've tried 75RR. The uncovered samples are the quickest and can be observed growing but quite slowly. The covered samples are very slow to crystallise, this is day 2 and have only progressed marginally since the top image was taken.

1st pic unmodified polarised illumination. Menthol dissolved in ethanol, a drop placed on slide and coverslip added. Crystals have formed around the edges of the coverslip and are now beginning to extend under the coverslip after 24hrs. This is a shot of that progression, I hope more will form in th...

Lovely detail and colours.

What an odd looking critter ID?

Dissolved in isopropyl alcohol. Polarised illumination using cheap plastic film for the upper filter, and a linear camera lens filter over the illuminator.
Objective: Zeiss Jena 6.3x apo
Microscope: Vickers M17
NFK 3.3x photo eyepiece

Nice work, thanks for sharing.

Yes Bob this mod would have little benefit in illumination systems with a fixed diffuser in the light path, possibly a marginal increase in brightness. The example above is also a worst case scenario for the (full Kohler) illumination on my microscope, normally the diffuser would be deployed at 4x a...

Lovely oblique/brightfield images. I know DIC is often the desired method for these subjects, but I still much prefer this type of shot. Thanks for sharing.

MichaelG, thanks. Yes my fingers survived without laceration, coffee drunk after the shaving

Hobbyst46 Yes its taken from a slide I made of Hypochaeris radicata pappus stained with Methyl Red. Thanks for the info on historical LED de-doming, no doubt where this all originated from.

As a side note I've recently been using UV-LOCA TP-2500F Glue for slide mounting subjects. This is a UV curing glue used for mounting screen covers on mobile phones and tablets. So far it is working very well. It doesn't harden fully so care needs to be taken with the amount used to avoid flooding o...

A while ago I bought a few high power Cree LED's and tested them while refurbishing a Vickers M17 microscope. I found the Cree XHP35 to have the most even phosphorous distribution and light pattern. Its a single die 12v LED with a die size of 3.45 x 3.45mm which closely matched the original filament...

The GS logo is 'Gillette and Siebert'. They were a UK company who produced good quality microscopes up until the late 80's. Their microscopes were mainly assembled from outsourced EU production parts. I have one of their scopes and the objectives are achromats like your example. I'm sure someone her...

Three to get you started:
http://www.microscopy-uk.org.uk/pond/
http://www.plingfactory.de/Science/Atla ... ista1.html
http://www.plingfactory.de/Science/Atla ... ifera.html

Very nice, well done.Was the specimen mounted or just a wet slide?

Fantastic images.

Lovely video, well done.

Scarodactyl, I don't use it always but it is useful sometimes for positioning a moving object (eyepiece is widefield, image is cropped), checking focus when adjusting a 40x collar, that sort of thing. I like to be able to glance up from the eyepiece and see what the camera is seeing. Michael I sugg...

I ordered an M50 2 days ago and awaiting arrival. I did my research and understood the camera can be triggered via EOS Utility 3 and USB tethering (as geo_man has working). My ageing 550D works flawlessly this way. The loss of a second trigger method isn't a concern, but I will be annoyed if Canon's...

Try this link (UK eBay, but should ship to Spain as its a Chinese seller): https://www.ebay.co.uk/itm/AC-Power-Ada ... SwBBpbrPXA

eBay item number 323470694306
HTH.

Hard to be certain, but looks like Colpodea.

Chris... I got the 90D because I want a higher resolution APS-C; a fully electronic shutter from live-view; and I wanted a camera that could do a quick focus stack sequence when used outdoors or for lower magnification close-ups. It does all three. Like all other cameras it is not "perfect", but th...

Well done Wes, another stunner.

Well done Karl, lovely to watch. Congrats on finding a complete colony to study, I've yet to find one. Thanks for sharing.

Great images and subject treatment. Are you happy with the Canon 90D? I'm considering buying one, any downsides?

Thanks for the kind words all. The critter tank has seen a rise in numbers with the longer daylight hours.

I just saw that you use a Vickers microscope! I have a good impression from them but very few poeple seem to actually use one. Bob Thanks for the reply. I'm really enjoying the Vickers M17 Bob, well worth the effort restoring it. Very heavy, no plastic gears, and a generous field of view. Despite h...