Search found 55 matches

by Chris Dee
Thu Jan 16, 2020 1:38 pm
Forum: Pictures and Videos
Topic: Testate Amoeba - Difflugia pyriformis
Replies: 4
Views: 158

Re: Testate Amoeba - Difflugia pyriformis

Cheers mintakax. Yes I was lucky to find an occupied one. In all honesty, if it wasn't moving I'd likely have dismissed it as a clump of detritus and moved on. :D Thanks for the input cuxlander. The overlay scale was made using a calibration slide so should be accurate. I studied the footage further...
by Chris Dee
Wed Jan 15, 2020 3:05 pm
Forum: Beginner's corner
Topic: Clean sample
Replies: 4
Views: 113

Re: Clean sample

I'll add the simplest method. Float a cover slip on the surface of a pond water sample and leave for 8 hours. Place a drop of clean sample water on a slide then carefully lift the cover slip off the surface and place on the slide. The hard bit is lifting the floating cover slip off without contamina...
by Chris Dee
Tue Jan 14, 2020 7:13 pm
Forum: Pictures and Videos
Topic: Rotifer (Squatinella mutica?)
Replies: 9
Views: 209

Re: Rotifer (Squatinella mutica?)

This sample came from a hollow rock in the critter tank. I scraped inside the hollow a little with the tip of a pipette then sucked up a sample. This was placed in a small vial and allowed to settle, then samples taken from the bottom of the vial for examination. I still get clumps of grit/detritus ...
by Chris Dee
Tue Jan 14, 2020 4:49 pm
Forum: Pictures and Videos
Topic: Testate Amoeba - Difflugia pyriformis
Replies: 4
Views: 158

Testate Amoeba - Difflugia pyriformis

There seems to be some uncertainty identifying Difflugia pyriformis but its the closest match I could find. Input welcome.

by Chris Dee
Tue Jan 14, 2020 4:39 pm
Forum: Pictures and Videos
Topic: Rotifer (Squatinella mutica?)
Replies: 9
Views: 209

Re: Rotifer (Squatinella mutica?)

Thanks for the encouragement mintakax. Always fun watching rotifers :)
by Chris Dee
Mon Jan 13, 2020 11:33 pm
Forum: Identification help
Topic: Rotifer cyst formation?
Replies: 0
Views: 34

Rotifer cyst formation?

Is this a rotifer in the process of forming a cyst or something else? Any help appreciated.

by Chris Dee
Mon Jan 13, 2020 6:50 pm
Forum: Camera systems and imaging
Topic: DSLR or Integrated?
Replies: 11
Views: 279

Re: DSLR or Integrated?

If you do go the DSLR route and plan to use it's live view function on a monitor via HDMI, do your research on live view output resolution. They can vary considerably and some models reduce the resolution while recording video. That was something I wished I'd known beforehand, but is seldom mentioned.
by Chris Dee
Mon Jan 13, 2020 6:36 pm
Forum: Pictures and Videos
Topic: Rotifer (Squatinella mutica?)
Replies: 9
Views: 209

Rotifer (Squatinella mutica?)

A good population of these in my critter tank at the moment, but quite difficult to film. Took several slides to find one that wasn't spinning non-stop. Excuse the hot-spot in the centre, I'm trying a new photo eyepiece which needs some work. AUMB8RWAw-Q Semi oblique lighting and 25x apo objective.
by Chris Dee
Sat Jan 11, 2020 2:15 pm
Forum: Microscopy accessories
Topic: 10x eyepiece for 23.2mm tube that fits this 33mm smartphone holder?
Replies: 9
Views: 144

Re: 10x eyepiece for 23.2mm tube that fits this 33mm smartphone holder?

If you know someone with a 3D printer they might be willing to design/print an adapter for you.
by Chris Dee
Sat Jan 11, 2020 2:10 pm
Forum: Home-made microscope adaptations
Topic: Slide Workbox
Replies: 7
Views: 178

Re: Slide Workbox

2012 date code, seem to be ok Bob but I'll keep an eye on them.
by Chris Dee
Wed Jan 08, 2020 8:59 pm
Forum: Pictures and Videos
Topic: Salt mix
Replies: 17
Views: 392

Re: Salt mix

Fantastic result, well done.
by Chris Dee
Wed Jan 08, 2020 8:43 pm
Forum: Home-made microscope adaptations
Topic: Slide Workbox
Replies: 7
Views: 178

Re: Slide Workbox

Absolutely Bob. Unfortunately I wasn't thinking of others when I designed it. Having bought a batch of 300 BH slides + slips in a sale, I'm resigned to not needing any others. I prioritised access for grasping by 2 edges with clumsy fingers. :) Not a fan of using tweezers.
by Chris Dee
Wed Jan 08, 2020 5:07 pm
Forum: Home-made microscope adaptations
Topic: Slide Workbox
Replies: 7
Views: 178

Slide Workbox

Designed this 3D printed slide workbox in an attempt to organise my limited deskspace. Holds 10 slides/cover slips and four vials. See description on Thingiverse if anyone wants to print one for themselves: https://www.thingiverse.com/thing:4091518
slide_box.jpg
slide_box.jpg (160.6 KiB) Viewed 178 times
by Chris Dee
Mon Jan 06, 2020 9:20 pm
Forum: Pictures and Videos
Topic: Green Hydra (Chlorohydra viridissima)
Replies: 7
Views: 730

Re: Green Hydra (Chlorohydra viridissima)

Hi mintakax. The slide used was a standard one with cover slip, though the detritus that invariably gets included in my pipette samples does tend to elevate the cover slip. I've tried well slides and find them awkward to work with, but I imagine they may work well with larger specimens such as this....
by Chris Dee
Sat Jan 04, 2020 8:14 pm
Forum: Illumination Techniques
Topic: LED vs. Halogen
Replies: 15
Views: 383

Re: LED vs. Halogen

Having used only 25w halogen my preference for LED may be skewed in some aspects. The high power LED I currently use enables me to capture at faster shutter speeds/lower ISO via liveview, with less radiated heat, reducing sample drying. The main reason I prefer LED however is rendering and contrast....
by Chris Dee
Tue Dec 10, 2019 5:55 pm
Forum: Pictures and Videos
Topic: Thrips says hello...
Replies: 3
Views: 408

Re: Thrips says hello...

Did you use a cover slip? You might get more detail without one.
by Chris Dee
Tue Dec 10, 2019 5:48 pm
Forum: Pictures and Videos
Topic: Dancing worms in on of my pond tanks probably Tubifex
Replies: 4
Views: 361

Re: Dancing worms in on of my pond tanks

Medusa aquaria :D Very nice Bob.
by Chris Dee
Wed Nov 27, 2019 1:23 pm
Forum: Beginner's corner
Topic: Microscope selection question
Replies: 9
Views: 649

Re: Microscope selection question

Personally I find my Chinese 60x Plan Achromat useful, but careful slide preparation is needed for best results.
by Chris Dee
Mon Nov 25, 2019 12:11 pm
Forum: Digital processing
Topic: Digitizing 35mm micro photography tranparency images
Replies: 7
Views: 1037

Re: Digitizing 35mm micro photography tranparency images

I digitised 300 family 35mm slides with a DIY setup of diffused flash/DSLR/macro lens/and slide mount. The results were acceptable but the biggest hurdle was ensuring the slides were clean and dust free beforehand. If not stated in the eBay service listing (sorry don't speak German) I'd enquire abou...
by Chris Dee
Sat Nov 23, 2019 10:51 am
Forum: Specimens, samples and slides
Topic: Containets
Replies: 2
Views: 327

Re: Containets

Search eBay for '25ml PET pots', you might find something suitable. Be aware most plastic containers have a 'spru' protrusion in the base (injection point for the plastic in the mould). This may or may no be a problem when observing samples in the container.
by Chris Dee
Sat Nov 23, 2019 10:42 am
Forum: Microscopes and optics
Topic: Moisture condensation in stereomicroscope
Replies: 7
Views: 409

Re: Moisture condensation in stereomicroscope

Given the location shown I'd suggest its caused by the warmth/humidity of your own breath using the microscope. Insulating that area with some foam tape should prevent it.
by Chris Dee
Sat Nov 23, 2019 10:30 am
Forum: Beginner's corner
Topic: thinking out loud here - moral thoughts
Replies: 21
Views: 1240

Re: thinking out loud here - moral thoughts

I was brought up not to waste things, so left over samples are returned. As humans we kill millions of microbes daily washing ourselves, walking, cooking, even just breathing. Stopping breathing will prevent further deaths and may increase microbe populations in the long run. ;)
by Chris Dee
Tue Nov 19, 2019 8:39 am
Forum: Illumination Techniques
Topic: Plan apo and dark field
Replies: 4
Views: 387

Re: Plan apo and dark field

I also encountered this with my 25x 0.65 NA objective. As 75RR states you'll need a larger custom patch. On my microscope I noticed a difference of just 1mm in radius had a large effect, and the margin of error for good contrast and dark background was narrow. Careful condenser height adjustment was...
by Chris Dee
Sun Nov 10, 2019 12:53 am
Forum: Identification help
Topic: Polychaeta (marine worm)
Replies: 3
Views: 768

Re: Polychaeta (marine worm)

Lovely video 75RR. Excellent detail, definitely Cthulu ;)
by Chris Dee
Mon Nov 04, 2019 10:30 pm
Forum: Pictures and Videos
Topic: Micro hedgehog
Replies: 10
Views: 1050

Re: Micro hedgehog

Cue little fella. :)
by Chris Dee
Mon Nov 04, 2019 10:20 pm
Forum: About the website, the magazine and this forum
Topic: Vetting newcomers
Replies: 15
Views: 2152

Re: Vetting newcomers

The Akismet extension allows to run phpBB posts, registrations and forms through the Akismet anti-spam service. https://www.phpbb.com/customise/db/extension/akismet/ Originally developed for preventing spam in Wordpress, but the above phpBB plugin will work here. Here's the Wikipedia entry for Akism...
by Chris Dee
Sun Nov 03, 2019 9:32 pm
Forum: Microscopes and optics
Topic: Need advice before buying first microscope
Replies: 6
Views: 843

Re: Need advice before buying first microscope

Looking forward to seeing some shots from your new microscope, keep us informed as to how you get on.
by Chris Dee
Sun Nov 03, 2019 9:25 pm
Forum: Pictures and Videos
Topic: Stylonychia
Replies: 5
Views: 654

Re: Stylonychia

Thanks mintakax. Its a toothy grin :)
by Chris Dee
Sun Nov 03, 2019 9:22 pm
Forum: Pictures and Videos
Topic: Ciliate in Methylcellulose
Replies: 13
Views: 1241

Re: Ciliate in Methylcellulose

Thanks for the info' Charlie, very useful. I like the Relis B.Brown recommendation and will try this ring method out. If I can get it to work well enough it would avoid having to apply MC to the whole sample, my only concern is how much it will elevate the cover slip. I recently bought a batch of ch...
by Chris Dee
Sun Nov 03, 2019 9:07 pm
Forum: Pictures and Videos
Topic: Green Hydra (Chlorohydra viridissima)
Replies: 7
Views: 730

Re: Green Hydra (Chlorohydra viridissima)

Thanks for the feedback 75RR. I agree, not the best colours for the subject, just something a little different. Pink next ;) The tank has a healthy population of green and brown Hydra, this one was rinsed back to its home. Thanks Charlie. I've a floating ring in the tank to keep an area free of duck...