Search found 121 matches

by thomas.schwarz
Sat May 02, 2020 5:36 pm
Forum: Identification help
Topic: Plant Bio 101
Replies: 1
Views: 987

Plant Bio 101

Hi friends, I remember "cytoplasm" and "endoplasmic reticulum" as pathways used in a cell to send things (from highschool). Enter reality: https://www.youtube.com/watch?v=LdJu4wI03gQ https://www.youtube.com/watch?v=O3khg4IO2iQ So I few questions asked there around communication within algae and a re...
by thomas.schwarz
Mon Apr 27, 2020 8:13 pm
Forum: Identification help
Topic: What on earth is this?
Replies: 7
Views: 1788

Re: What on earth is this?

Thanks!

....Any idea why they have evolved to join at the corners? And why does it go roughly from bigger to smaller? Are new members born exclusively at one end and die excludively at the other? Links about their lifecycle?
by thomas.schwarz
Sat Apr 25, 2020 2:35 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

hmmm.....just was thinking about 75RR's comment that paramount is parfocality between the eyepieces and the camera sensor..... so that is an easy experiment, and the winner hands down is "C", the 150 mm L Tube (with very thick adapter). So maybe the answer here is that I am just so unfamiliar with t...
by thomas.schwarz
Sat Apr 25, 2020 1:58 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

OK firends, the grand experiment.... Variations for distance between upper projection eyepiece lens and camera sensor are A) 83mm, B) 105mm or c) 150mm. Please let me know your choice A, B or C, or in between? Experiment Overview - https://www.youtube.com/watch?v=FO2QEB9RNEQ A 40x - https://www.yout...
by thomas.schwarz
Wed Apr 22, 2020 9:31 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

...just when you thought it was safe to put my mtv-3 on ebay.... ...here it is again, same slides, this time with mtv-3 phototube, no lense, 40x field of view becomes 2.3 mm across instead of .12 mm accross. But there is so much new and valuable resolution! Why? and yes there is loss of parfocality ...
by thomas.schwarz
Wed Apr 22, 2020 8:18 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

Hello Friends, Here is a comparison of a Zeiss .17 mm coverslip (guaranteed to within .005 mm) and a generic coverslip betweeen .17 and .13 mm thick. https://www.youtube.com/watch?v=rGDbb5UH4gE I created the two permanent mounts with the same dandelion seed hairs, with entellan mounting medium. At 4...
by thomas.schwarz
Tue Apr 21, 2020 7:06 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

OK... here is calibration slide video with the OEM photo tube, GH4 Panasonic Camera: https://www.youtube.com/watch?v=FXMqjLceWuI&feature=youtu.be My current theory is that I had dirt in my projection eyepiece (on and between the two lenses) and after cleaning it, my microscope is performing normally...
by thomas.schwarz
Sun Apr 19, 2020 9:37 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

...started making videos to explore/critique my current solution, based on your comments, and I find that I am more confused than ever, I cannot effectively demonstrate that the shorter tube (the MTV-3 tube without additional optics) improves resolution. But it does negatively effect parfocality (th...
by thomas.schwarz
Sat Apr 18, 2020 10:08 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

major major break through here. issue fixed by shortening photo tube by 30% (was 15 CM now about 10). I had the OEM photo tube that came with the microscope and was used for that 35 MM Film Camera. Fix was to go (back) to the MTV-3 Olympus photo tube. And interesting, that photo tube came with a len...
by thomas.schwarz
Sat Apr 18, 2020 11:27 am
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

Thanks for the feedback. So I am simplifying the problem domain by sticking to only bright field. And also in this video, showing the specimen with all obectives: 4x dplan, 10x dplan, 20x splan, 40x splan. My next step will be to try the regular eyepiece in the photo tube, look more into the objecti...
by thomas.schwarz
Mon Apr 13, 2020 6:57 am
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

hello friends, first off thank you very much for your help. I think that this is a very complex domain (validate correct and optimal performance/resolution for a given microscope). in short, i will order the diatom slide and follow up, starting with bright field. but in parallel, lets see if my opti...
by thomas.schwarz
Tue Apr 07, 2020 9:13 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

Hi just wanted to follow up here... ...using a very thin specimen, just one little drop of water, I do have improved resolution and focusing with my 40x objective. please let me know if you think that this is about as good as it gets? or if I should get more resolution? I am still waiting for my cal...
by thomas.schwarz
Tue Apr 07, 2020 5:47 pm
Forum: Pictures and Videos
Topic: Swamps come alive.
Replies: 11
Views: 1142

Re: Swamps come alive.

Beautiful. Thank you.
by thomas.schwarz
Sat Mar 28, 2020 12:28 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

super interesting discussion.... there is a lot that i can do to prove out the theory/hypothesis, that my current focusing woes are significantly impacted by cover slide thickness.... how about using a prepared slide? just did that... ...so there i have no idea about slide thickness (doah!) but sure...
by thomas.schwarz
Fri Mar 27, 2020 7:38 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

Re: 40x SPlan woes

great feedback and tips. thank you so much. i tried some of the ideas including extensive cleaning of objectives and condenser. of course there is always more that one can do, but with just the objective and 80% of the condenser lenses, there is some small improvement! thank you. then about the dist...
by thomas.schwarz
Thu Mar 26, 2020 12:22 am
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 6023

40x SPlan woes

Hi friends, Now on my second 40x SPlan für my Olympus BH-2 BHS.... here is a video I made to demonstrate how poorly my 40X Splan focuses in brightfield and in DIC. Before I posted that it focused poorly in dark field. But it actually focuses very poorly with all illumination and contrast techniques....
by thomas.schwarz
Sun Mar 22, 2020 3:20 pm
Forum: Pictures and Videos
Topic: Vorticella during division
Replies: 8
Views: 3049

Re: Vorticella during division

sinabro wrote:
Thu Mar 19, 2020 1:25 am
Thank you all


1. Correct dic set up. see ==> http://www.frankshospitalworkshop.com/e ... Manual.pdf


UMREAL!!!!! I have been looking for that doc!!!!!! I will forward that to some friends.....

Thanks!
by thomas.schwarz
Wed Mar 18, 2020 4:07 pm
Forum: Pictures and Videos
Topic: Vorticella during division
Replies: 8
Views: 3049

Re: Vorticella during division

Very interesting.... is this a well known process for vortecella? Did it tgrn become two? Also really clrar for 40x. Any tips on getting a clear image with 40x dic?
by thomas.schwarz
Wed Mar 18, 2020 4:03 pm
Forum: Pictures and Videos
Topic: Toxarium undulatum
Replies: 13
Views: 3088

Re: Toxarium undulatum

Interesting. So much like algae. How do you differentiate this diatom from algae?
by thomas.schwarz
Sun Mar 15, 2020 5:48 pm
Forum: Pictures and Videos
Topic: Litonotus lamella ?
Replies: 3
Views: 2795

Re: Litonotus lamella ?

Wow awesome info.... so i would like to familiarize myself with this language distribution of extrusomes, a good count of kineties, etc. A quick internet search implies that extrusomes are ejectable membrane-bound organelle. But what are keneties? Or better yet, rather than feeding me fish, can you ...
by thomas.schwarz
Sat Mar 14, 2020 3:55 pm
Forum: Pictures and Videos
Topic: Litonotus lamella ?
Replies: 3
Views: 2795

Litonotus lamella ?

BH2 BHS 20x and 40x SPlan DIC with UCD, GH4 4K You like? Any tips for better focus/ deeper field of focus? https://www.youtube.com/watch?v=DGSp5PoYFq4 https://www.youtube.com/watch?v=y2qIwMBKWoc&feature=youtu.be https://www.youtube.com/watch?v=08xlVX66Gnc I am starting to think this not a Litonotus,...
by thomas.schwarz
Fri Mar 13, 2020 11:57 pm
Forum: Microscopes and optics
Topic: Broken Microscope
Replies: 20
Views: 4665

Re: Broken Microscope

Unreal that you had that doc. Where is the OP?
by thomas.schwarz
Fri Mar 13, 2020 11:03 pm
Forum: Identification help
Topic: a blob with a tail?
Replies: 4
Views: 3339

Re: a blob with a tail?

Amazing. Ive never seen anything like that. No-one here has seen this beautiful micro whale?
by thomas.schwarz
Fri Mar 13, 2020 10:59 pm
Forum: Identification help
Topic: Not an amoeba?
Replies: 9
Views: 4302

Re: Not an amoeba?

Beautiful. Proof of alien life on Earth.
by thomas.schwarz
Sat Mar 07, 2020 9:29 pm
Forum: Identification help
Topic: Two microbes, doing.... what?
Replies: 0
Views: 6146

Two microbes, doing.... what?

Predation Competition Commensalism Mutalism (sex) ? My best guess is attempted predation, but was it acutally sex between a worm like being and a mulosk like counterpart? pt 1 https://www.youtube.com/watch?v=WF-uSZqWW-c pt 2 https://www.youtube.com/watch?v=1StlPPP59Sg&t=175s pt 3 https://www.youtube...
by thomas.schwarz
Sun Mar 01, 2020 8:52 pm
Forum: Pictures and Videos
Topic: Synedra ulna
Replies: 6
Views: 1937

Re: Synedra ulna

beautiful
by thomas.schwarz
Sun Mar 01, 2020 8:50 pm
Forum: Identification help
Topic: UFO Unidentified object!
Replies: 8
Views: 2303

Re: UFO Unidentified object!

awesome. great dark field image! no idea from where this being came. ...truly a mystery for all us here....

he/she surely came from ancestors who came on a meteor, the only question is, were those ancestors crystals or procaryotes of sorts....
by thomas.schwarz
Sat Feb 29, 2020 7:25 pm
Forum: Identification help
Topic: Proales decipiens
Replies: 1
Views: 1233

Proales decipiens

https://www.youtube.com/watch?v=jvUbLTSfq_Y

I am new to identification of microbes. Would anyone say that this is not a Proales decipiens?

ALl I have to work with is the book "Das Leben im Wasserprodfen" are there good web sites for identifying microbes?
by thomas.schwarz
Sat Feb 29, 2020 7:21 pm
Forum: Pictures and Videos
Topic: First DIC, Gratitude
Replies: 5
Views: 1541

Re: First DIC, Gratitude

Right, BH2 BHS, GH4 Camera, 20x SPlan, UCD Condenser Special thank you to @Tom Jones for all the tips, and Boss at dealcorner.com for pristine intermediate tube analyser, 20x S Plan and original BH-2 phototube.... And thank you Oliver Kim for rekindling my love of plant biology and all things cellul...