Search found 121 matches
- Sat May 02, 2020 5:36 pm
- Forum: Identification help
- Topic: Plant Bio 101
- Replies: 1
- Views: 2199
Plant Bio 101
Hi friends, I remember "cytoplasm" and "endoplasmic reticulum" as pathways used in a cell to send things (from highschool). Enter reality: https://www.youtube.com/watch?v=LdJu4wI03gQ https://www.youtube.com/watch?v=O3khg4IO2iQ So I few questions asked there around communication within algae and a re...
- Mon Apr 27, 2020 8:13 pm
- Forum: Identification help
- Topic: What on earth is this?
- Replies: 7
- Views: 4210
Re: What on earth is this?
Thanks!
....Any idea why they have evolved to join at the corners? And why does it go roughly from bigger to smaller? Are new members born exclusively at one end and die excludively at the other? Links about their lifecycle?
....Any idea why they have evolved to join at the corners? And why does it go roughly from bigger to smaller? Are new members born exclusively at one end and die excludively at the other? Links about their lifecycle?
- Mon Apr 27, 2020 9:06 am
- Forum: Identification help
- Topic: What on earth is this?
- Replies: 7
- Views: 4210
- Sat Apr 25, 2020 2:35 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
hmmm.....just was thinking about 75RR's comment that paramount is parfocality between the eyepieces and the camera sensor..... so that is an easy experiment, and the winner hands down is "C", the 150 mm L Tube (with very thick adapter). So maybe the answer here is that I am just so unfamiliar with t...
- Sat Apr 25, 2020 1:58 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
OK firends, the grand experiment.... Variations for distance between upper projection eyepiece lens and camera sensor are A) 83mm, B) 105mm or c) 150mm. Please let me know your choice A, B or C, or in between? Experiment Overview - https://www.youtube.com/watch?v=FO2QEB9RNEQ A 40x - https://www.yout...
- Wed Apr 22, 2020 9:31 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
...just when you thought it was safe to put my mtv-3 on ebay.... ...here it is again, same slides, this time with mtv-3 phototube, no lense, 40x field of view becomes 2.3 mm across instead of .12 mm accross. But there is so much new and valuable resolution! Why? and yes there is loss of parfocality ...
- Wed Apr 22, 2020 8:18 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
Hello Friends, Here is a comparison of a Zeiss .17 mm coverslip (guaranteed to within .005 mm) and a generic coverslip betweeen .17 and .13 mm thick. https://www.youtube.com/watch?v=rGDbb5UH4gE I created the two permanent mounts with the same dandelion seed hairs, with entellan mounting medium. At 4...
- Tue Apr 21, 2020 7:06 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
OK... here is calibration slide video with the OEM photo tube, GH4 Panasonic Camera: https://www.youtube.com/watch?v=FXMqjLceWuI&feature=youtu.be My current theory is that I had dirt in my projection eyepiece (on and between the two lenses) and after cleaning it, my microscope is performing normally...
- Sun Apr 19, 2020 9:37 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
...started making videos to explore/critique my current solution, based on your comments, and I find that I am more confused than ever, I cannot effectively demonstrate that the shorter tube (the MTV-3 tube without additional optics) improves resolution. But it does negatively effect parfocality (th...
- Sat Apr 18, 2020 10:08 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
major major break through here. issue fixed by shortening photo tube by 30% (was 15 CM now about 10). I had the OEM photo tube that came with the microscope and was used for that 35 MM Film Camera. Fix was to go (back) to the MTV-3 Olympus photo tube. And interesting, that photo tube came with a len...
- Sat Apr 18, 2020 11:27 am
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
Thanks for the feedback. So I am simplifying the problem domain by sticking to only bright field. And also in this video, showing the specimen with all obectives: 4x dplan, 10x dplan, 20x splan, 40x splan. My next step will be to try the regular eyepiece in the photo tube, look more into the objecti...
- Mon Apr 13, 2020 6:57 am
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
hello friends, first off thank you very much for your help. I think that this is a very complex domain (validate correct and optimal performance/resolution for a given microscope). in short, i will order the diatom slide and follow up, starting with bright field. but in parallel, lets see if my opti...
- Tue Apr 07, 2020 9:13 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
Hi just wanted to follow up here... ...using a very thin specimen, just one little drop of water, I do have improved resolution and focusing with my 40x objective. please let me know if you think that this is about as good as it gets? or if I should get more resolution? I am still waiting for my cal...
- Tue Apr 07, 2020 5:47 pm
- Forum: Pictures and Videos
- Topic: Swamps come alive.
- Replies: 11
- Views: 4284
Re: Swamps come alive.
Beautiful. Thank you.
- Sat Mar 28, 2020 12:28 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
super interesting discussion.... there is a lot that i can do to prove out the theory/hypothesis, that my current focusing woes are significantly impacted by cover slide thickness.... how about using a prepared slide? just did that... ...so there i have no idea about slide thickness (doah!) but sure...
- Fri Mar 27, 2020 7:38 pm
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
Re: 40x SPlan woes
great feedback and tips. thank you so much. i tried some of the ideas including extensive cleaning of objectives and condenser. of course there is always more that one can do, but with just the objective and 80% of the condenser lenses, there is some small improvement! thank you. then about the dist...
- Thu Mar 26, 2020 12:22 am
- Forum: Microscopes and optics
- Topic: 40x SPlan woes
- Replies: 53
- Views: 19405
40x SPlan woes
Hi friends, Now on my second 40x SPlan für my Olympus BH-2 BHS.... here is a video I made to demonstrate how poorly my 40X Splan focuses in brightfield and in DIC. Before I posted that it focused poorly in dark field. But it actually focuses very poorly with all illumination and contrast techniques....
- Sun Mar 22, 2020 3:20 pm
- Forum: Pictures and Videos
- Topic: Vorticella during division
- Replies: 8
- Views: 6068
Re: Vorticella during division
sinabro wrote: ↑Thu Mar 19, 2020 1:25 amThank you all
1. Correct dic set up. see ==> http://www.frankshospitalworkshop.com/e ... Manual.pdf
UMREAL!!!!! I have been looking for that doc!!!!!! I will forward that to some friends.....
Thanks!
- Wed Mar 18, 2020 4:07 pm
- Forum: Pictures and Videos
- Topic: Vorticella during division
- Replies: 8
- Views: 6068
Re: Vorticella during division
Very interesting.... is this a well known process for vortecella? Did it tgrn become two? Also really clrar for 40x. Any tips on getting a clear image with 40x dic?
- Wed Mar 18, 2020 4:03 pm
- Forum: Pictures and Videos
- Topic: Toxarium undulatum
- Replies: 13
- Views: 6467
Re: Toxarium undulatum
Interesting. So much like algae. How do you differentiate this diatom from algae?
- Sun Mar 15, 2020 5:48 pm
- Forum: Pictures and Videos
- Topic: Litonotus lamella ?
- Replies: 3
- Views: 4252
Re: Litonotus lamella ?
Wow awesome info.... so i would like to familiarize myself with this language distribution of extrusomes, a good count of kineties, etc. A quick internet search implies that extrusomes are ejectable membrane-bound organelle. But what are keneties? Or better yet, rather than feeding me fish, can you ...
- Sat Mar 14, 2020 3:55 pm
- Forum: Pictures and Videos
- Topic: Litonotus lamella ?
- Replies: 3
- Views: 4252
Litonotus lamella ?
BH2 BHS 20x and 40x SPlan DIC with UCD, GH4 4K You like? Any tips for better focus/ deeper field of focus? https://www.youtube.com/watch?v=DGSp5PoYFq4 https://www.youtube.com/watch?v=y2qIwMBKWoc&feature=youtu.be https://www.youtube.com/watch?v=08xlVX66Gnc I am starting to think this not a Litonotus,...
- Fri Mar 13, 2020 11:57 pm
- Forum: Microscopes and optics
- Topic: Broken Microscope
- Replies: 20
- Views: 10556
Re: Broken Microscope
Unreal that you had that doc. Where is the OP?
- Fri Mar 13, 2020 11:03 pm
- Forum: Identification help
- Topic: a blob with a tail?
- Replies: 4
- Views: 4947
Re: a blob with a tail?
Amazing. Ive never seen anything like that. No-one here has seen this beautiful micro whale?
- Fri Mar 13, 2020 10:59 pm
- Forum: Identification help
- Topic: Not an amoeba?
- Replies: 9
- Views: 7610
Re: Not an amoeba?
Beautiful. Proof of alien life on Earth.
- Sat Mar 07, 2020 9:29 pm
- Forum: Identification help
- Topic: Two microbes, doing.... what?
- Replies: 0
- Views: 6929
Two microbes, doing.... what?
Predation Competition Commensalism Mutalism (sex) ? My best guess is attempted predation, but was it acutally sex between a worm like being and a mulosk like counterpart? pt 1 https://www.youtube.com/watch?v=WF-uSZqWW-c pt 2 https://www.youtube.com/watch?v=1StlPPP59Sg&t=175s pt 3 https://www.youtube...
- Sun Mar 01, 2020 8:52 pm
- Forum: Pictures and Videos
- Topic: Synedra ulna
- Replies: 6
- Views: 3850
Re: Synedra ulna
beautiful
- Sun Mar 01, 2020 8:50 pm
- Forum: Identification help
- Topic: UFO Unidentified object!
- Replies: 8
- Views: 5303
Re: UFO Unidentified object!
awesome. great dark field image! no idea from where this being came. ...truly a mystery for all us here....
he/she surely came from ancestors who came on a meteor, the only question is, were those ancestors crystals or procaryotes of sorts....
he/she surely came from ancestors who came on a meteor, the only question is, were those ancestors crystals or procaryotes of sorts....
- Sat Feb 29, 2020 7:25 pm
- Forum: Identification help
- Topic: Proales decipiens
- Replies: 1
- Views: 2205
Proales decipiens
https://www.youtube.com/watch?v=jvUbLTSfq_Y
I am new to identification of microbes. Would anyone say that this is not a Proales decipiens?
ALl I have to work with is the book "Das Leben im Wasserprodfen" are there good web sites for identifying microbes?
I am new to identification of microbes. Would anyone say that this is not a Proales decipiens?
ALl I have to work with is the book "Das Leben im Wasserprodfen" are there good web sites for identifying microbes?
- Sat Feb 29, 2020 7:21 pm
- Forum: Pictures and Videos
- Topic: First DIC, Gratitude
- Replies: 5
- Views: 3417
Re: First DIC, Gratitude
Right, BH2 BHS, GH4 Camera, 20x SPlan, UCD Condenser Special thank you to @Tom Jones for all the tips, and Boss at dealcorner.com for pristine intermediate tube analyser, 20x S Plan and original BH-2 phototube.... And thank you Oliver Kim for rekindling my love of plant biology and all things cellul...