MicrobeHunter.com Microscopy Forum

I checked this morning: Actually the difference in focus between 100x oil objectives of 160 and 170 is very small. So you shouldn't smash anything.

To question 1: I have used both in Orthoplan with no problems. In priciple, you are right with the parfoaclity. The most difficult objective to focus is 100x oil immersion. So it is most convenient if you have that objective and the one with the second highest magnification (e.g. 40x) of the same tu...

Photos of the microscope? Why? There are some here:

viewtopic.php?f=12&t=9617&p=82035#p82035
viewtopic.php?f=24&t=9420&p=80594#p80594

Have you posted photos of it anywhere? Absolutely not. This won't happen for a long time. First of all, I must buy a better camera to take any pictures which could be published. The second problem is that my led lighting is not powerful enough for phaco so I have to build a better system for that. ...

Though Leitz Orthoplan is a very versatile modular system, It seems practically impossible to extend it for DIC. I have only seen a photo of Leitz DIC condenser, However never a single one in Ebay. The DIC objectives in Ebay are all for infinite system, which would, in turn, need a special objective...

For what purpose there was PCB in immersion oils?

Tried (and failed) to use a 1mm hex machine screw, but the cap is too wide.

How about narrowing the outer diameter of the screw? If you don't have a lathe, you could try to fit the screw into a drilling machine and use a file. And get some heat

The original idea in that thread could probably be adapted though, by using a hex key to make the broach

Tried (and failed) to use a 1mm hex machine screw, but the cap is too wide.

So the circulated screw head must be male. Sorry, I didn't realize this at first.

Can't see the photo very clearly. But isn't it a female screw? At least the non-circulated screw looks like female which would need male wrench?

Never used Nikon. However, I would first try an inch size wrench. As you probably know there are two series of hex keys. One for metric sizes and one for inch sizes. Both sets of keys may be bought a low price at local hardware store.

Everything is just fine . Thanks for the valuable list! I probably would have bought a couple of these if I was aware of these are available. And if these would be too thick, it is always possible to refine them to appropriate diameter.

So far so good.. However, the outer diameter is also limited in this particular case.

Thanks for the good idea! I never come to think about that. However, maybe this was of more fun. At least it was free.

I recently bought a phase contrast condenser for my Orthoplan. There were no centering wrenches with it so I had to think how to resolve this problem in my workshop. As my solution might have wider interest, I present my results here. The starting point is to find metal tube of appropriate diameter....

Isopropanol works fine. It is also usable in molecular biology instead of ethanol e.g. in DNA precipitation and plasmid prep kits.

For histology: https://pubmed.ncbi.nlm.nih.gov/14534624/

I completely agree the first part. huge investment of time at the expense of almost everything else This is requirement is a result of the competition I described. Personally I feel it is a bad life strategy - even some kind of sickness. There are many other nice and interesting things in life in ad...

Interesting questions and I am very happy to hear you have passion for science and research. I have always had the feeling I am not very smart. Despite of that I have background in biochemistry and molecular biology and have more than 25 years of experience in lab work. In my career there has been g...

And yes, it looks exactly like the glass plate in the video.

I am very pleased to see my photos has some importance here :) Maybe I exaggerated it is absolutely necessary. However, according to my experience, it helps a lot. Sorry my poor English as I am not so familiar with all terminolgy. The round glass plate (A in picture) is a diffuser, would the better ...

I really appreciate this fine work. I assume the needle is fixed into a micromanipulator? Pictures of those would be nice to see, especially if they are self made.

This looks all very fine. However, as I know nothing about diatomes, could you explain for what purpose you actually use these? Is "needle" solid or are these capillaries? If capillaries, you could make microinjections to egg cells . I am just curious...

I also happen to have the Leitz Orthoplan Vario Tube

About Variotube: is it really usefull or is it empty magnification? Any experiences?

The Zeiss Axioplan was probably equipped with a 100W mercury lamp or 75W Xenon, both of which provide powerful excitation at these wavelengths. So the LEDs face an ancient but strong competitor... ;) Commercial LEDs for fluorescence microscopy are very expensive, so any DIY investment is potentiall...

Staining cells with DAPI and Acridine orange works. However, the intensity is not so high compared to what I remember it was at universitys Zeiss Axioplan. However, I did fluorescence microscopy only a very short time many years ago and only to check the function of some antibodies. I used them in i...

Which lamp do you use for fluorescence?

I put it here viewtopic.php?f=24&t=9420

As it was asked here http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=16&t=9139&start=30 which lamp I use, I put the answer here as it is quite off topic in the place the question is asked. I have home-made led conversions in both transmitted and epi-fluorescence lightning. The transmit...

This photo was taken by an exchange student in the Pavlov First State Medical University of St. Petersburg in the year 2000. The medical students are studying histology using Biolam microscopes. Note the innovative ball lamp in the middle of the desk.

Interesting Lomo experiences: Part 4 The Legendary Biolam The Lomo Biolam is a basic biological microscope which was very common as a student microscope at schools and Universities in Finland during 70’s and 80’s. When I attended microbiology course in early 90’s they already had new Olympus microsc...