Search found 86 matches

by Leitzcycler
Wed Jul 08, 2020 7:36 am
Forum: Microscopes and optics
Topic: Leitz Dialux questions: Mixing 160/170 objectives and best objectives for water organisms
Replies: 5
Views: 59

Re: Leitz Dialux questions: Mixing 160/170 objectives and best objectives for water organisms

I checked this morning: Actually the difference in focus between 100x oil objectives of 160 and 170 is very small. So you shouldn't smash anything.
by Leitzcycler
Tue Jul 07, 2020 9:03 pm
Forum: Microscopes and optics
Topic: Leitz Dialux questions: Mixing 160/170 objectives and best objectives for water organisms
Replies: 5
Views: 59

Re: Leitz Dialux questions: Mixing objectives, darkfield, and best objectives for water organisms

To question 1: I have used both in Orthoplan with no problems. In priciple, you are right with the parfoaclity. The most difficult objective to focus is 100x oil immersion. So it is most convenient if you have that objective and the one with the second highest magnification (e.g. 40x) of the same tu...
by Leitzcycler
Tue Jun 30, 2020 8:39 am
Forum: Microscopes and optics
Topic: Best DIC options?
Replies: 57
Views: 1381

Re: Best DIC options?

by Leitzcycler
Tue Jun 30, 2020 8:00 am
Forum: Microscopes and optics
Topic: Best DIC options?
Replies: 57
Views: 1381

Re: Best DIC options?

Have you posted photos of it anywhere? Absolutely not. This won't happen for a long time. First of all, I must buy a better camera to take any pictures which could be published. The second problem is that my led lighting is not powerful enough for phaco so I have to build a better system for that. ...
by Leitzcycler
Tue Jun 30, 2020 7:36 am
Forum: Microscopes and optics
Topic: Best DIC options?
Replies: 57
Views: 1381

Re: Best DIC options?

Though Leitz Orthoplan is a very versatile modular system, It seems practically impossible to extend it for DIC. I have only seen a photo of Leitz DIC condenser, However never a single one in Ebay. The DIC objectives in Ebay are all for infinite system, which would, in turn, need a special objective...
by Leitzcycler
Tue Jun 16, 2020 7:38 am
Forum: Specimens, samples and slides
Topic: Cargill immersion oil "B" probably 1960s
Replies: 13
Views: 576

Re: Cargill immersion oil "B" probably 1960s

For what purpose there was PCB in immersion oils? :shock:
by Leitzcycler
Sun Jun 14, 2020 4:50 am
Forum: Microscopes and optics
Topic: HELP: Missing centering tool for Nikon Optiphot objectives
Replies: 39
Views: 1462

Re: HELP: Missing centering tool for Nikon Optiphot objectives

Tried (and failed) to use a 1mm hex machine screw, but the cap is too wide.
How about narrowing the outer diameter of the screw? If you don't have a lathe, you could try to fit the screw into a drilling machine and use a file. And get some heat :)
by Leitzcycler
Sun Jun 14, 2020 4:41 am
Forum: Microscopes and optics
Topic: HELP: Missing centering tool for Nikon Optiphot objectives
Replies: 39
Views: 1462

Re: HELP: Missing centering tool for Nikon Optiphot objectives

The original idea in that thread could probably be adapted though, by using a hex key to make the broach
:D
by Leitzcycler
Sat Jun 13, 2020 7:45 pm
Forum: Microscopes and optics
Topic: HELP: Missing centering tool for Nikon Optiphot objectives
Replies: 39
Views: 1462

Re: HELP: Missing centering tool for Nikon Optiphot objectives

Tried (and failed) to use a 1mm hex machine screw, but the cap is too wide.
So the circulated screw head must be male. Sorry, I didn't realize this at first.
by Leitzcycler
Sat Jun 13, 2020 7:01 pm
Forum: Microscopes and optics
Topic: HELP: Missing centering tool for Nikon Optiphot objectives
Replies: 39
Views: 1462

Re: HELP: Missing centering tool for Nikon Optiphot objectives

Can't see the photo very clearly. But isn't it a female screw? At least the non-circulated screw looks like female which would need male wrench?
by Leitzcycler
Sat Jun 13, 2020 6:50 pm
Forum: Microscopes and optics
Topic: HELP: Missing centering tool for Nikon Optiphot objectives
Replies: 39
Views: 1462

Re: HELP: Missing centering tool for Nikon Optiphot objectives

Never used Nikon. However, I would first try an inch size wrench. As you probably know there are two series of hex keys. One for metric sizes and one for inch sizes. Both sets of keys may be bought a low price at local hardware store.
by Leitzcycler
Thu Jun 11, 2020 10:47 am
Forum: Home-made microscope adaptations
Topic: How to make miniature square wrenches
Replies: 10
Views: 418

Re: How to make miniature square wrenches

Everything is just fine :) . Thanks for the valuable list! I probably would have bought a couple of these if I was aware of these are available. And if these would be too thick, it is always possible to refine them to appropriate diameter.
by Leitzcycler
Thu Jun 11, 2020 10:09 am
Forum: Home-made microscope adaptations
Topic: How to make miniature square wrenches
Replies: 10
Views: 418

Re: How to make miniature square wrenches

So far so good.. However, the outer diameter is also limited in this particular case.
by Leitzcycler
Wed Jun 10, 2020 11:22 am
Forum: Home-made microscope adaptations
Topic: How to make miniature square wrenches
Replies: 10
Views: 418

Re: How to make miniature square wrenches

Thanks for the good idea! I never come to think about that. However, maybe this was of more fun. At least it was free.
by Leitzcycler
Wed Jun 10, 2020 7:29 am
Forum: Home-made microscope adaptations
Topic: How to make miniature square wrenches
Replies: 10
Views: 418

How to make miniature square wrenches

I recently bought a phase contrast condenser for my Orthoplan. There were no centering wrenches with it so I had to think how to resolve this problem in my workshop. As my solution might have wider interest, I present my results here. The starting point is to find metal tube of appropriate diameter....
by Leitzcycler
Tue Jun 09, 2020 2:28 pm
Forum: Beginner's corner
Topic: Alcohol/surgical spirit?
Replies: 6
Views: 258

Re: Alcohol/surgical spirit?

Isopropanol works fine. It is also usable in molecular biology instead of ethanol e.g. in DNA precipitation and plasmid prep kits.

For histology: https://pubmed.ncbi.nlm.nih.gov/14534624/
by Leitzcycler
Tue Jun 09, 2020 12:29 pm
Forum: Introduce yourself
Topic: Bio Major (advice on future career and academic goals)
Replies: 28
Views: 712

Re: Bio Major (advice on future career and academic goals)

I completely agree the first part. huge investment of time at the expense of almost everything else This is requirement is a result of the competition I described. Personally I feel it is a bad life strategy - even some kind of sickness. There are many other nice and interesting things in life in ad...
by Leitzcycler
Tue Jun 09, 2020 9:32 am
Forum: Introduce yourself
Topic: Bio Major (advice on future career and academic goals)
Replies: 28
Views: 712

Re: Bio Major (advice on future career and academic goals)

Interesting questions and I am very happy to hear you have passion for science and research. I have always had the feeling I am not very smart. Despite of that I have background in biochemistry and molecular biology and have more than 25 years of experience in lab work. In my career there has been g...
by Leitzcycler
Thu Jun 04, 2020 8:14 pm
Forum: Beginner's corner
Topic: LOMO 2.5 Objective Focusing
Replies: 48
Views: 926

Re: LOMO 2.5 Objective Focusing

And yes, it looks exactly like the glass plate in the video.
by Leitzcycler
Thu Jun 04, 2020 8:09 pm
Forum: Beginner's corner
Topic: LOMO 2.5 Objective Focusing
Replies: 48
Views: 926

Re: LOMO 2.5 Objective Focusing

I am very pleased to see my photos has some importance here :) Maybe I exaggerated it is absolutely necessary. However, according to my experience, it helps a lot. Sorry my poor English as I am not so familiar with all terminolgy. The round glass plate (A in picture) is a diffuser, would the better ...
by Leitzcycler
Mon Jun 01, 2020 7:14 pm
Forum: Specimens, samples and slides
Topic: Build Your Own Needel Puller, Part Two
Replies: 18
Views: 662

Re: Build Your Own Needel Puller, Part Two

I really appreciate this fine work. I assume the needle is fixed into a micromanipulator? Pictures of those would be nice to see, especially if they are self made.
by Leitzcycler
Tue May 26, 2020 7:23 pm
Forum: Specimens, samples and slides
Topic: Build Your Own Needel Puller, Part Two
Replies: 18
Views: 662

Re: Build Your Own Needel Puller, Part Two

This looks all very fine. However, as I know nothing about diatomes, could you explain for what purpose you actually use these? Is "needle" solid or are these capillaries? If capillaries, you could make microinjections to egg cells :) . I am just curious...
by Leitzcycler
Tue May 19, 2020 5:24 pm
Forum: Microscopes and optics
Topic: Best way to assess condition of microscope objective optics?
Replies: 8
Views: 241

Re: Best way to assess condition of microscope objective optics?

I also happen to have the Leitz Orthoplan Vario Tube
About Variotube: is it really usefull or is it empty magnification? Any experiences?
by Leitzcycler
Mon May 18, 2020 7:53 pm
Forum: My microscope
Topic: Orthoplan lightning
Replies: 6
Views: 350

Re: Orthoplan lightning

The Zeiss Axioplan was probably equipped with a 100W mercury lamp or 75W Xenon, both of which provide powerful excitation at these wavelengths. So the LEDs face an ancient but strong competitor... ;) Commercial LEDs for fluorescence microscopy are very expensive, so any DIY investment is potentiall...
by Leitzcycler
Mon May 18, 2020 7:33 pm
Forum: My microscope
Topic: Orthoplan lightning
Replies: 6
Views: 350

Re: Orthoplan lightning

Staining cells with DAPI and Acridine orange works. However, the intensity is not so high compared to what I remember it was at universitys Zeiss Axioplan. However, I did fluorescence microscopy only a very short time many years ago and only to check the function of some antibodies. I used them in i...
by Leitzcycler
Mon May 18, 2020 11:10 am
Forum: My microscope
Topic: Orthoplan lightning
Replies: 6
Views: 350

Re: Orthoplan lightning

:)
by Leitzcycler
Mon May 18, 2020 11:07 am
Forum: My microscope
Topic: Orthoplan lightning
Replies: 6
Views: 350

Orthoplan lightning

As it was asked here http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=16&t=9139&start=30 which lamp I use, I put the answer here as it is quite off topic in the place the question is asked. I have home-made led conversions in both transmitted and epi-fluorescence lightning. The transmit...
by Leitzcycler
Sun May 17, 2020 6:55 pm
Forum: Microscopes and optics
Topic: LOMO as a brand
Replies: 25
Views: 1223

Re: LOMO as a brand

This photo was taken by an exchange student in the Pavlov First State Medical University of St. Petersburg in the year 2000. The medical students are studying histology using Biolam microscopes. Note the innovative ball lamp in the middle of the desk.
by Leitzcycler
Sun May 17, 2020 6:52 pm
Forum: Microscopes and optics
Topic: LOMO as a brand
Replies: 25
Views: 1223

Re: LOMO as a brand

Interesting Lomo experiences: Part 4 The Legendary Biolam The Lomo Biolam is a basic biological microscope which was very common as a student microscope at schools and Universities in Finland during 70’s and 80’s. When I attended microbiology course in early 90’s they already had new Olympus microsc...