Search found 255 matches

by Leitzcycler
Thu Mar 28, 2024 8:32 pm
Forum: Home-made microscope adaptations
Topic: Looking for a NICE dust cover
Replies: 20
Views: 4795

Re: Looking for a NICE dust cover

I found a place online that makes outdoor covers for grills and furniture, and also had a heavy clear material for taxi seat covers. Looks very nice. Could you give a link to the company? If postage to my place would be reasonable I am interested. However, the cover pattern/cutting instructions wou...
by Leitzcycler
Sat Dec 30, 2023 9:08 pm
Forum: Microscopes and optics
Topic: Nikon Diaphot project
Replies: 5
Views: 1853

Re: Nikon Diaphot project

How thick is the original black round objective plate? My stage is missing the plate.
by Leitzcycler
Wed Dec 27, 2023 9:04 am
Forum: Illumination Techniques
Topic: Does phase contrast make sense for its price?
Replies: 49
Views: 32383

Re: Does phase contrast make sense for its price?

Thanks, another question:
bodies that are undergoing more rapid brownian movement
What about the movement of the organelles inside the yeast cells? Is that brownian movement as well or rather a result of cellular function and metabolism? Or both?
by Leitzcycler
Tue Dec 26, 2023 7:51 pm
Forum: Illumination Techniques
Topic: Does phase contrast make sense for its price?
Replies: 49
Views: 32383

Re: Does phase contrast make sense for its price?

If you look at DIC images of yeast they are maybe the best you could dream for:
https://www.youtube.com/watch?v=PTGYLYru2Sw
My question: what are the small, round and fast moving objects between the yeast cells?
by Leitzcycler
Wed Dec 20, 2023 8:38 pm
Forum: Beginner's corner
Topic: Tools for dissecting really tiny spiders and insects
Replies: 6
Views: 4042

Re: Tools for dissecting really tiny spiders and insects

I used micro spring scissors for dissecting. I purchased mine from here https://www.finescience.com/en-US/Produ ... ors?pg=all
You may also find them in Ebay for much less price. However I don't know about the quality.
by Leitzcycler
Thu Nov 23, 2023 3:13 pm
Forum: Microscopes and optics
Topic: Nikon Diaphot project
Replies: 5
Views: 1853

Re: Nikon Diaphot project

I have a PDF of exploded diagrams, not sure it would be helpful but if you want it I can send a link to you. Yes, It would be nice to have anyway. Please, send it. You may not need to disassemble it. Just soak the whole thing in solvent, then re-lubricate externally (let lubricant drip in). That is...
by Leitzcycler
Thu Nov 23, 2023 2:07 pm
Forum: Microscopes and optics
Topic: Nikon Diaphot project
Replies: 5
Views: 1853

Nikon Diaphot project

Nikon Diaphot x-y stage control needs cleaning and relubrication. However, I don't know how to disassemble this handle. Has anyone done this and could give some hints how to take this apart?
by Leitzcycler
Wed Jul 19, 2023 6:26 am
Forum: Microscopy accessories
Topic: Making glass knives from microscope slides
Replies: 6
Views: 1896

Re: Making glass knives from microscope slides

I have done this a lot. However, I have always used glass strips specifically made for EM ultra microtomy. You first cut a square and then divide it half to make two triangles. You may use an ordinary glass knife and pliers to get a reasonable good quality knife for light microscopy. There seems to ...
by Leitzcycler
Tue Mar 21, 2023 8:00 am
Forum: Important announcements
Topic: Leitz Orthoplan - How to adjust focus tension?
Replies: 27
Views: 35867

Re: Leitz Orthoplan - How to adjust focus tension?

I recommend total disassembly, mineral spirit and toothbrush/wipes, lubrication and reassembly for the x-y stage. I have done that myself and it is not a big job. Old heated lubricant may stuck again later or does not work as it should lubricating the surfaces. For iris diaphragm I have immersed the...
by Leitzcycler
Sat Mar 04, 2023 1:07 pm
Forum: Important announcements
Topic: Leitz Orthoplan - How to adjust focus tension?
Replies: 27
Views: 35867

Re: Leitz Orthoplan - How to adjust focus tension?

We are talking about oil only if you have the focus block opened. There is no way to get oil inside Orthoplan focus block without taking it off the stand. This may, in turn, lead to collimation problems. Xylene is only used when the focus block is completely serviced. That means all the parts will b...
by Leitzcycler
Wed Mar 01, 2023 8:14 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

The tube (in your picture) might be originally for another type of epi illuminator nosepiece. My tube is 76,5mm of total lenght. If the length is ok you may use it with ploemopak.
by Leitzcycler
Wed Mar 01, 2023 8:00 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

I have just a simple tube. In your tube in the picture there is probably an iris diaphragm. Maybe useful in some applications. Ploemopak has its own diaphragm. The tube will prevent light scattering. The diffusion filter makes the lightning more even and important with many more primitive lightning ...
by Leitzcycler
Fri Feb 17, 2023 10:15 am
Forum: Important announcements
Topic: Leitz Orthoplan - How to adjust focus tension?
Replies: 27
Views: 35867

Re: Leitz Orthoplan - How to adjust focus tension?

Try pouring a tiny amount of mineral spirits around the focus knob, David. You may get lucky. I don't think it will penetrate inside. In the similar situation I would probably take the focusing block out of the stand and add some lubricant e.g. WD40 into the coarse focusing parts. This might work f...
by Leitzcycler
Tue Jan 31, 2023 1:06 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

Since i lost the Ploemopak auction, sadly,
I am so sorry. It went for a good price. Don't worry, they will come. :)
by Leitzcycler
Sun Jan 29, 2023 1:15 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

I don't know if you Phaco objectives would work well. I would guess the phaco ring would do some harm for the light intensity. Not sure what will happen, never tried myself.
by Leitzcycler
Sun Jan 29, 2023 12:53 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

So this Ploemopak for sale can be used with epi-fluerescence too if i later make the transition for LED? Yes. If you are seriously interested in fluorescence microscopy, that Ploemopak for affordable price would be a very good investment. Personally I have no experience with transmitted fluorescenc...
by Leitzcycler
Sat Jan 28, 2023 9:12 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

In case you would like to play a game :mrgreen: https://www.ebay.de/itm/155361922357?hash=item242c4acd35:g:h8YAAOSwlgdjxT49&amdata=enc%3AAQAHAAAAoA0XXWvz9PjQxqcjNAd4b%2FpqfEw8Rtzjs8k4ByJnaFRli0vmnIJxXc8wr2W7IBMkYIV7Hsw%2FMcDHhlTXg3qPzLekee3tTNqlAuGAI1iewsFe63itM%2BqdEPaBmDzUhYX7YdQCHI3yYyFkivMg5R5SZ...
by Leitzcycler
Sat Jan 28, 2023 8:07 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

Your Orthoplan is already configured for transient light fluorescence.
Sorry, I didn't realize there are some filters. Be careful with the mercury lamp as I think it may be even more dangerous if used with transient light.
by Leitzcycler
Sat Jan 28, 2023 7:21 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

The price on that ploemopak is normal?
It is overpriced. I think mine was around 250-300 EUR with three filter cubes.
by Leitzcycler
Sat Jan 28, 2023 11:03 am
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

Well, you have a very nice high quality scope. Now collecting parts and accessories will start and probably will cost at least double the price of the scope anyway :) Here are some suggestions how to construct led lighting for fluorescence https://www.microbehunter.com/microscopy-forum/viewtopic.php...
by Leitzcycler
Thu Jan 26, 2023 8:23 pm
Forum: My microscope
Topic: Orthoplan lightning
Replies: 25
Views: 10524

Re: Orthoplan lightning

My latest version.

Version 2.0.jpg
Version 2.0.jpg (196.48 KiB) Viewed 4217 times
by Leitzcycler
Thu Jan 26, 2023 7:47 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

There are both lamp houses in your pictures. If you use fluorescence, you need a ploemopak with filter cubes. I highly recommend led conversion. The focus block in Orthoplan is very complicated and is a problem IF it needs servicing. If focus turns smoothly, no problem. These are good reading, you w...
by Leitzcycler
Thu Jan 26, 2023 1:03 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9864

Re: Help with decision on Leitz Orthoplan

After about 5 years of experience with Orthoplan: It is a very high quality scope with plenty of objectives and parts available in Ebay. However, DIC parts are extremely rare and hard to find (I don't have myself). It is a rather large stand and not easily portable so it needs lot of space. I do mos...
by Leitzcycler
Sat Dec 03, 2022 8:23 pm
Forum: Microscopes and optics
Topic: Leitz filter cube numbering
Replies: 0
Views: 518

Leitz filter cube numbering

I have found three data sheets describing Leitz filter cube specifications and have the following information H3: BP 420-490 RKP 510 LP 520 K3: BP 470-490 RKP 510 LP 520 K2: extremely narrow band blue at 495 nm It appears that K2 information is only available in the oldest(?) datasheet. And accordin...
by Leitzcycler
Mon Nov 21, 2022 7:48 pm
Forum: Microscopes and optics
Topic: Questions about Zeiss objectives on a Olympus microscope
Replies: 10
Views: 1736

Re: Questions about Zeiss objectives on a Olympus microscope

I always put oil on the slide and then turn the nose piece. Just some fine-focusing after that. No mess at all. :) Though I think the other way is more professional, I have found this quick and easy and most comfortable for me.
by Leitzcycler
Thu Nov 10, 2022 5:41 pm
Forum: Specimens, samples and slides
Topic: Embedding cheese for slicing
Replies: 6
Views: 1861

Re: Embedding cheese for slicing

Or you may construct a poor mans cryo microtome: buy a surplus ice cream cabinet and put your microtome and tools in there :)
by Leitzcycler
Thu Nov 10, 2022 10:31 am
Forum: Specimens, samples and slides
Topic: Embedding cheese for slicing
Replies: 6
Views: 1861

Re: Embedding cheese for slicing

If you are able to freeze your microtome, making cryo sections sounds an excellent method to try. Some thoughts: You may freeze your microtome in the freezer (a few hours may be ok) and then try to keep it cold during your work by wrapping most part of it into some plastic foam. It would perhaps be ...
by Leitzcycler
Wed Nov 09, 2022 7:30 pm
Forum: Specimens, samples and slides
Topic: Embedding cheese for slicing
Replies: 6
Views: 1861

Re: Embedding cheese for slicing

Very interesting project indeed. Your staining looks absolutely great!

Just came to my mind if agar would work.