Search found 253 matches

by BramHuntingNematodes
Wed Jul 08, 2020 1:18 am
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 14
Views: 109

Re: Please help a microscope noob with objectives

I got a raspberry pi on the old dynazoom right now. It runs the camera and some software. Very useful for adjusting all the camera settings and capturing images. Probably could hook up some stepper motors here and there to dials and knobs and get an automated scope. I hear there are some neat techni...
by BramHuntingNematodes
Tue Jul 07, 2020 6:39 pm
Forum: Beginner's corner
Topic: 'Noise' when viewing at higher magnifications
Replies: 12
Views: 117

Re: 'Noise' when viewing at higher magnifications

Keep at it. I have fiddled with my microscope a long while before getting comfortable enough with it to know how to set it up instinctively.
by BramHuntingNematodes
Tue Jul 07, 2020 6:19 pm
Forum: Beginner's corner
Topic: 'Noise' when viewing at higher magnifications
Replies: 12
Views: 117

Re: 'Noise' when viewing at higher magnifications

It happens when I use the 40x objective, with either of the two eyepieces I have, which are 16x and 5x. No! I have no idea how to use oil, appropriately or inappropriately! What should I be doing? Whenever the objectives get higher magnifications and numerical apertures, everything also gets more f...
by BramHuntingNematodes
Tue Jul 07, 2020 5:04 pm
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 14
Views: 109

Re: Please help a microscope noob with objectives

Also to echo Zuul's sentiments, if this project is just for your personal enthusiasm for 3D printing go for it, but the end result will undoubtedly be a much inferior product to an old cast iron and machined brass microscope stand made by and for professionals that could be had for $50 or less.
by BramHuntingNematodes
Tue Jul 07, 2020 5:01 pm
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 14
Views: 109

Re: Please help a microscope noob with objectives

Yeah you should be able to get a whole set of old B&L achromat lenses pretty cheap they are 160mm tube length but 35 and 2/3mm parfocal length. Think you can move the stage up 9mm?
by BramHuntingNematodes
Tue Jul 07, 2020 1:57 pm
Forum: Beginner's corner
Topic: 'Noise' when viewing at higher magnifications
Replies: 12
Views: 117

Re: 'Noise' when viewing at higher magnifications

Hi, I was hoping someone might help me work out what's going on... When I looked through the lower magnification objectives I can see things pretty well, but when I get up to the highest ones, suddenly it's like I'm trying to see through layers of murk. how high we talking about? I assume oil is be...
by BramHuntingNematodes
Tue Jul 07, 2020 4:54 am
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

I love that guy's lighting setup with the busted out spray paint top.
by BramHuntingNematodes
Tue Jul 07, 2020 3:46 am
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

I somehow figured length D to be about 187.8mm which puts the overall focal length of the thing at -33.24mm.
by BramHuntingNematodes
Tue Jul 07, 2020 3:38 am
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

The doublet has to be assembly 21 if it is immediately in front of the objectives. It's the shared fixed position negative corrector lens. I have seen some "dense crown" glass with specs close to one of the elements, but only within the ranges and not at the ideal. In any case, at least one of thos...
by BramHuntingNematodes
Tue Jul 07, 2020 3:32 am
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

The doublet has to be assembly 21 if it is immediately in front of the objectives. It's the shared fixed position negative corrector lens. I have seen some "dense crown" glass with specs close to one of the elements, but only within the ranges and not at the ideal. In any case, at least one of those...
by BramHuntingNematodes
Mon Jul 06, 2020 6:38 pm
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

Hmm maybe I can manufacture a replacement doublet after all if these specifications held true in production. Requires some relatively specific dense crown and dense flint glass lenses, the double concave of which has a somewhat unusual focal length for the 7/16ths diameter. Might require some speci...
by BramHuntingNematodes
Mon Jul 06, 2020 3:20 pm
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 92
Views: 5152

Re: Breaking the baffling B & L Balplan buffalo.

Hmm maybe I can manufacture a replacement doublet after all if these specifications held true in production.
by BramHuntingNematodes
Mon Jul 06, 2020 3:11 pm
Forum: Beginner's corner
Topic: Help needed in deciding on first time microscope
Replies: 13
Views: 414

Re: Help needed in deciding on first time microscope

Much of the book is an index or reference to specific stains or preparations used by slidemakers past. The first section has useful general advice. You might also try Gray's other book, the handbook of basic microtechnique, for an excellent overview of microscopy and slide preparation.
by BramHuntingNematodes
Sat Jul 04, 2020 7:45 pm
Forum: Beginner's corner
Topic: Help needed in deciding on first time microscope
Replies: 13
Views: 414

Re: Help needed in deciding on first time microscope

If you would like a nearly exhaustive treatment I recommend:
https://archive.org/details/microtomistsform00gray

Vital stains are a compromise between being good at staining and not killing/ needing fixed tissue. They are simple enough to use. You just add a drop to your wet mount.
by BramHuntingNematodes
Sat Jul 04, 2020 3:10 pm
Forum: Beginner's corner
Topic: The Dirty Cover Glass Blues
Replies: 25
Views: 760

Re: The Dirty Cover Glass Blues

It's usually not too problematic unless you are doing darkfield and then every little speck, smudge and wipe mark shows up pretty starkly
by BramHuntingNematodes
Fri Jul 03, 2020 4:32 am
Forum: My microscope
Topic: Flat Field Dynazoom Operational
Replies: 14
Views: 848

Re: Flat Field Dynazoom Operational

Nothing wrong with that. At only .65 too. According to the catalogues, they made a 50X .80 planachromat too but I have never seen one, just a 50X .80 oil planfluorite, which I have never seen in a catalogue. I see someone is selling a 80x .85 Planachromat in the BF/DF Met. style. One of our Balplan...
by BramHuntingNematodes
Fri Jul 03, 2020 4:05 am
Forum: My microscope
Topic: Flat Field Dynazoom Operational
Replies: 14
Views: 848

Re: Flat Field Dynazoom Operational

Wow, what color would they even use for it, like orange?
by BramHuntingNematodes
Fri Jul 03, 2020 3:45 am
Forum: Microscopy accessories
Topic: B&L Phase Contrast Condenser Sliders
Replies: 12
Views: 474

Re: B&L Phase Contrast Condenser Sliders

I believe the Balplan, like the earlier B&L phase system with inserts, has a special purpose condenser with a huge slot in it above the iris but below the condenser lens, as well as an additional set of centering screws.
by BramHuntingNematodes
Fri Jul 03, 2020 3:32 am
Forum: My microscope
Topic: Flat Field Dynazoom Operational
Replies: 14
Views: 848

Re: Flat Field Dynazoom Operational

The latter three were fully zoomed. 40x planachromat.

Also the gastrotrich was wiggling about quite a bit
by BramHuntingNematodes
Fri Jul 03, 2020 12:47 am
Forum: My microscope
Topic: Flat Field Dynazoom Operational
Replies: 14
Views: 848

Re: Flat Field Dynazoom Operational

What a difference a lens can make! The old lens was a 16mm m12 lens for a 1/2" sensor. My sensor is only 2/5", so I got a new lens, decided to make a 12mm also to capture the whole FOV now that my lenses are plan. I destroyed my first LED driver and so am using a flashlight as the replacement arrive...
by BramHuntingNematodes
Thu Jul 02, 2020 11:50 pm
Forum: Beginner's corner
Topic: The Dirty Cover Glass Blues
Replies: 25
Views: 760

Re: The Dirty Cover Glass Blues

Oh, and if you ever want to test your skills with a real challenge, try cleaning up a dirty camera sensor.
by BramHuntingNematodes
Thu Jul 02, 2020 11:48 pm
Forum: Beginner's corner
Topic: The Dirty Cover Glass Blues
Replies: 25
Views: 760

Re: The Dirty Cover Glass Blues

Alcohol works better than soap? No, not exactly. Ideally you want to triple-team your cover slips with a mixture of dish soap, water and alcohol. I like about 10-15% alcohol by volume, just a little squirt of dish soap and water. A spray bottle works pretty good here, or poke a needle hole into the...
by BramHuntingNematodes
Thu Jul 02, 2020 2:29 pm
Forum: Pictures and Videos
Topic: Garden Plant Preparing to Seed
Replies: 2
Views: 316

Re: Garden Plant Preparing to Seed

dare I say it, these pictures are slowly improving, Don. Looks good!
by BramHuntingNematodes
Wed Jul 01, 2020 11:57 pm
Forum: Identification help
Topic: ID freshwater microbes from wetlands 100x
Replies: 6
Views: 463

Re: ID freshwater microbes from wetlands 100x

1. A very flexible hypotrich ciliate. It's probably a urostylid, but we don't see it closely enough to be sure. 2. A ciliate, probably in the genus Loxophyllum , but it could also be Bryophyllum (a closer view would be helpful) 3. A rotifer in the genus Platyias . *Blows imaginary smoke from tips o...
by BramHuntingNematodes
Wed Jul 01, 2020 4:33 pm
Forum: Microscopes and optics
Topic: Fixed Köhler
Replies: 19
Views: 653

Re: Fixed Köhler

I think it's somewhat relevant as Kohler himself describes the field diaphragm, the "blenden" close to his light source, as part of his light train whereas this system seems to lack one. Then again, Kohler, a man after my own heart, also talks about using a cardboard cutout for this and improvising ...
by BramHuntingNematodes
Wed Jul 01, 2020 2:57 pm
Forum: Microscopes and optics
Topic: Fixed Köhler
Replies: 19
Views: 653

Re: Fixed Köhler

Koehler is such a trope in amateur microscopy anymore. People who have never looked in a microscope launch themselves after Koehler illumination, as though it is going to make or break the quality of a microscope. Every self illuminated microscope ever made was designed as closely as possible to ac...
by BramHuntingNematodes
Wed Jul 01, 2020 1:30 pm
Forum: Beginner's corner
Topic: Help needed. Want to buy microscope for my girlfriend
Replies: 2
Views: 156

Re: Help needed. Want to buy microscope for my girlfriend

Maybe look around for a zeiss standard. I could pretty readily get one stateside for that much and it's much more romantic
by BramHuntingNematodes
Wed Jul 01, 2020 4:11 am
Forum: Introduce yourself
Topic: Microphotography newbie
Replies: 24
Views: 751

Re: Microphotography newbie

Well, a nice thing about a 571 is that they are pretty simple to use-- no obscure adjustments made to several knobs, dials, levers and switches all at once. I am still experimenting with camera set ups myself, but you might want to start cheaply and work your way up. Also, for stereo samples, a lot ...
by BramHuntingNematodes
Tue Jun 30, 2020 7:32 pm
Forum: Microscopes and optics
Topic: Focus loss upon microscope stage movement
Replies: 6
Views: 283

Re: Focus loss upon microscope stage movement

Is the slide getting listed off of the stage at any point? I have a slightly bent arm on my stage and this has given me some terrible frustrations only for me to notice the slide is slightly lifted, tilted on the stage.
by BramHuntingNematodes
Tue Jun 30, 2020 6:31 pm
Forum: My microscope
Topic: Amscope T720QB and Phase Contrast
Replies: 9
Views: 361

Re: Amscope T720QB and Phase Contrast

There is no switch to select the light to either the eyepiece or trinocular. Amscope rep replied to me that "The microscope does not have a switch to select light to go to the eyepiece. You can either use the microscope eyepieces or the camera but not both at the same time." I am not sure what they...