Search found 575 matches

by BramHuntingNematodes
Mon Nov 30, 2020 5:43 am
Forum: For forum members who want to buy and sell equipment
Topic: GIA Gemolite MkV 60x gemological stereo microscope refurbished
Replies: 6
Views: 176

Re: GIA Gemolite MkV 60x gemological stereo microscope refurbished

The price seems pretty good for them Gem-O-Lites, but danged if that isn't the most expensive stand for a Stereozoom.
by BramHuntingNematodes
Mon Nov 30, 2020 5:40 am
Forum: Beginner's corner
Topic: ...awakening a Leitz SM microscope...
Replies: 6
Views: 121

Re: ...awakening a Leitz SM microscope...

For Kohler you need a field lens to collimate your LED, but not necessarily a field iris (Kohler used a cardboard stop I think?). After that your Berek will be fine, and has that additional iris that can serve as field diaphragm. It's just like any flip top condenser in its use I should think. I'm n...
by BramHuntingNematodes
Sun Nov 29, 2020 4:06 pm
Forum: Beginner's corner
Topic: ...awakening a Leitz SM microscope...
Replies: 6
Views: 121

Re: ...awakening a Leitz SM microscope...

The field will be small but the resolution should be excellent. This is a superb microscope, and if you ever get a new one it will likely not be as sharp. I don't find the lack of plan corrections to be overly troublesome, even in photography. This is especially true when being able focus on an orga...
by BramHuntingNematodes
Sun Nov 29, 2020 8:12 am
Forum: Miscellaneous
Topic: Desktop electron microscope
Replies: 2
Views: 94

Re: Desktop electron microscope

This is an electric microscope and not an electron microscope-- the vintage one I have seen (university research machine) occupied the better part of the room it was in.
by BramHuntingNematodes
Sun Nov 29, 2020 8:05 am
Forum: Specimens, samples and slides
Topic: Slide Prep Techniques for Nematodes
Replies: 4
Views: 84

Re: Slide Prep Techniques for Nematodes

Oh also you introduce the specimen to the fixative immediately, like as in the creatures are still alive and in the root knot
by BramHuntingNematodes
Sun Nov 29, 2020 8:02 am
Forum: Specimens, samples and slides
Topic: Slide Prep Techniques for Nematodes
Replies: 4
Views: 84

Re: Slide Prep Techniques for Nematodes

I use formalin but hear that glutaraldehyde on its own or as an adjunct works better(for nematodes). I clear with histoclear as it smells better than xylene, and omnimount pairs perfectly with it. The H-E stain would be about the closest thing to a general anatomical stain-- it's all some microtomis...
by BramHuntingNematodes
Wed Nov 25, 2020 2:34 am
Forum: Sandbox
Topic: Do we need Phase Contrast?
Replies: 37
Views: 2160

Re: Do we need Phase Contrast?

Dialux seems like a pretty competent platform and Leitz seems to have had an extensive phase line at one time or another I don't think there would be a big advantage in switching
by BramHuntingNematodes
Mon Nov 23, 2020 4:42 pm
Forum: Illumination Techniques
Topic: How can one improve polarization?
Replies: 9
Views: 181

Re: How can one improve polarization?

Can you obtain extinction?
by BramHuntingNematodes
Mon Nov 23, 2020 4:01 pm
Forum: Illumination Techniques
Topic: How can one improve polarization?
Replies: 9
Views: 181

Re: How can one improve polarization?

Do you have both a polarizer and an analyzer wet up? The effect is not subtle.
by BramHuntingNematodes
Mon Nov 23, 2020 3:32 pm
Forum: My microscope
Topic: Objective lens image quality
Replies: 2
Views: 113

Re: Objective lens image quality

To paraphrase Tolstoy, every good objective is good in the same way, and every bad objective is bad in its own way.
by BramHuntingNematodes
Mon Nov 23, 2020 4:32 am
Forum: Sandbox
Topic: Do we need Phase Contrast?
Replies: 37
Views: 2160

Re: Do we need Phase Contrast?

Oh also you can often do dark field and circular oblique with a rotary phase condenser and normal bf objectives in addition to regular bf, so that's neat
by BramHuntingNematodes
Mon Nov 23, 2020 4:28 am
Forum: Sandbox
Topic: Do we need Phase Contrast?
Replies: 37
Views: 2160

Re: Do we need Phase Contrast?

Yes, you can look at bright field with a phase setup, sometimes with small reduction in resolution as a result of interference the phase plate introduces. The manufacturer phase objectives do a p. good job of minimizing this loss I tell you a homemade phase plate I have been having some real trouble...
by BramHuntingNematodes
Wed Nov 18, 2020 5:06 pm
Forum: Pictures and Videos
Topic: Can anyone ID this?
Replies: 8
Views: 270

Re: Can anyone ID this?

There are some wacky marine nematodes that have furry coatings, but I think you might only very rarely encounter them if not looking for them specifically.
by BramHuntingNematodes
Mon Nov 16, 2020 7:17 pm
Forum: Microscopes and optics
Topic: Need help selecting Eyepieces
Replies: 5
Views: 239

Re: Need help selecting Eyepieces

OEM eyepieces are preferable. I think just about everything on Ali Express is a gamble, probably even more so for precision optical components.
by BramHuntingNematodes
Mon Nov 16, 2020 4:29 pm
Forum: Camera systems and imaging
Topic: Microscope compatible with Sony nex-5n camera
Replies: 10
Views: 331

Re: Microscope compatible with Sony nex-5n camera

I think the APS-C size is good for direct imaging as well. You shouldn't need either a reduction lens like you might for a smaller sensor, nor a projection lens employed for 35mm formats. You might encounter slight vignetting, but you will probably still have significantly over-sampled the cropped a...
by BramHuntingNematodes
Sun Nov 15, 2020 9:40 pm
Forum: Home-made microscope adaptations
Topic: Homemade phase annuli
Replies: 13
Views: 2649

Re: Homemade phase annuli

I have a broken AO finite phase objective, but I can't bring myself to break it open to try to salvage the phase plate as it is so beautiful. Let me know if you find success.
by BramHuntingNematodes
Sun Nov 15, 2020 7:12 am
Forum: Home-made microscope adaptations
Topic: Homemade phase annuli
Replies: 13
Views: 2649

Re: Homemade phase rings

So I am following along with great interest the paper mentioned previously by Apo, Bennett, Jupnik, Osterberg and Richards "Phase Microscopy"(1946), as well as some of the previous and later citing work. I can produce the annuli pretty readily now, so on to the phase rings. Were going to put them cl...
by BramHuntingNematodes
Sat Nov 14, 2020 5:52 pm
Forum: Microscopes and optics
Topic: Broken Microscope
Replies: 20
Views: 4665

Re: Broken Microscope

There are no bad AO lenses to my knowledge.
by BramHuntingNematodes
Sat Nov 14, 2020 5:49 am
Forum: Microscopes and optics
Topic: Broken Microscope
Replies: 20
Views: 4665

Re: Broken Microscope

It is my feeling that this gizmo must twist, push or pull to switch viewing from the front tube to the vertical tube. I have seen other AO 60s with an auxiliary tube and they also had this part.
by BramHuntingNematodes
Fri Nov 13, 2020 3:29 pm
Forum: My microscope
Topic: Things I've seen from the past but haven't seen available now.
Replies: 10
Views: 845

Re: Things I've seen from the past but haven't seen available now.

I think the availability of brass shim rings is the same or better maybe just not marketed to the microscope set as much as they aren't as widely used now that manufacturers have settled on some standards and intermixing of lenses is not as easy. They got laminated brass shim rings for bearing races...
by BramHuntingNematodes
Thu Nov 12, 2020 9:47 pm
Forum: Camera systems and imaging
Topic: B&L Stereozoom Photography ?
Replies: 25
Views: 1320

Re: B&L Stereozoom Photography ?

Hmm yeah looks better when zoomed in. It looks pretty good though!
by BramHuntingNematodes
Thu Nov 12, 2020 6:26 pm
Forum: Illumination Techniques
Topic: Achieving oblique illumination with motic ba310e
Replies: 18
Views: 1107

Re: Achieving oblique illumination with motic ba310e

There is a solid aluminum foil tape used for duct work, no not "duct tape" foil tape, that can be put over masks plastic or cardboard to great effect. I used it once on a iris diaphragm I made out of paper and it worked a charm.
by BramHuntingNematodes
Thu Nov 12, 2020 6:05 pm
Forum: Sandbox
Topic: Do we need Phase Contrast?
Replies: 37
Views: 2160

Re: Do we need Phase Contrast?

Mmm, if scarodactyl still has a pair after I win the lottery I may inquire after those.
by BramHuntingNematodes
Thu Nov 12, 2020 3:53 pm
Forum: Sandbox
Topic: Do we need Phase Contrast?
Replies: 37
Views: 2160

Re: Do we need Phase Contrast?

Regrettably, Bausch & Lomb did not get into phase too deeply. Although just the default dark system seems to be all, the flat field and planachromat versions are first class. I finally found a 20x Planachromat Phase objective. It was tricky as it had a Leica sticker on it, and I think I probably ha...
by BramHuntingNematodes
Wed Nov 11, 2020 5:44 pm
Forum: Microscopes and optics
Topic: So, will I get the optics clean (Wild drawing tube)?
Replies: 6
Views: 306

Re: So, will I get the optics clean (Wild drawing tube)?

You'll be fine if nothing else you will learn something
by BramHuntingNematodes
Tue Nov 10, 2020 3:56 pm
Forum: Home-made microscope adaptations
Topic: LED Lighting and optical resolution questions
Replies: 86
Views: 2930

Re: LED Lighting and optical resolution questions

Yeah bulbs and arrays can have as bad of artifacts as filaments, but that's what kohler was trying to mitigate in the first place. A diffuser might be a good idea for a bulb, but then also kohler using the diffuser surface as your light source
by BramHuntingNematodes
Tue Nov 10, 2020 3:09 pm
Forum: Miscellaneous
Topic: Mineralogy, Metallurgy
Replies: 11
Views: 439

Re: Mineralogy, Metallurgy

Yeah I was going to say a petro setup is pretty doable but back in school days you had grad students making those thin sections had all kinds of equipment long lab hours take a couple quarters to have enough good in situ sphene crystals to write a paper on
by BramHuntingNematodes
Tue Nov 10, 2020 3:06 pm
Forum: Home-made microscope adaptations
Topic: LED Lighting and optical resolution questions
Replies: 86
Views: 2930

Re: LED Lighting and optical resolution questions

I usually start with a field lens with point source lighting. I needed a diffuser for an led bulb once that had a large array of leds that cast uneven shadows, but if you don't see those than a diffuser probably won't help.. I think a test slide is often employed when trying to determine resolution...
by BramHuntingNematodes
Tue Nov 10, 2020 1:28 pm
Forum: Home-made microscope adaptations
Topic: LED Lighting and optical resolution questions
Replies: 86
Views: 2930

Re: LED Lighting and optical resolution questions

I usually start with a field lens with point source lighting. I needed a diffuser for an led bulb once that had a large array of leds that cast uneven shadows, but if you don't see those than a diffuser probably won't help.. I think a test slide is often employed when trying to determine resolution....