Search found 1538 matches
- Tue Mar 26, 2024 12:18 am
- Forum: Microscopes and optics
- Topic: Substituting LED bulb for incandescent bulb
- Replies: 7
- Views: 366
Re: Substituting LED bulb for incandescent bulb
It's never too bright
- Mon Mar 25, 2024 6:43 pm
- Forum: Microscopes and optics
- Topic: Substituting LED bulb for incandescent bulb
- Replies: 7
- Views: 366
Re: Substituting LED bulb for incandescent bulb
The wiring'll be fine, you already noted that it will pull half the wattage. There are sometimes issues when filament position is critical, but I don't think it's too sensitive with the optilume.
- Mon Mar 18, 2024 5:49 am
- Forum: Microscopy accessories
- Topic: Anti-Static for lenses.
- Replies: 26
- Views: 1390
Re: Anti-Static for lenses.
I seem to recall one of the diatomists here using that anti static gun to help when positioning things.
- Tue Dec 05, 2023 12:06 am
- Forum: Beginner's corner
- Topic: Good Stereo Microscope?
- Replies: 36
- Views: 22760
Re: Good Stereo Microscope?
I like the b&l sz7 but you might want to stay away if you aren't a hands in kind of person as almost all of them need a little work. An sz4 is cheaper and they made a million of them. Sometimes can be had for very little. They're not the most superb imaging available but they are madly convenient. T...
- Thu Oct 12, 2023 2:36 pm
- Forum: Microscopes and optics
- Topic: Different Nikon 20/0.75 160 Plan Apos
- Replies: 14
- Views: 3718
Re: Different Nikon 20/0.75 160 Plan Apos
I don't think Nikon ever made a cheap plan apo.
- Wed Oct 11, 2023 12:25 am
- Forum: Beginner's corner
- Topic: Equipment for the microscopy hobby
- Replies: 11
- Views: 3619
Re: Equipment for the microscopy hobby
Good forceps
- Thu May 25, 2023 4:58 am
- Forum: Microscopes and optics
- Topic: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
- Replies: 16
- Views: 2607
Re: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
Yeah you oughta talk to Apochronaut about buying direct if you're contemplating laying down six or seven thou on a microscope. A little more legwork maybe but might be worth your time.
- Thu May 25, 2023 2:50 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2524
Re: Types of Phase Contrast and when to use which type
Yeah the black knobbly base. Some are Bausch and Lomb, some are Leica.
- Thu May 25, 2023 2:18 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2524
Re: Types of Phase Contrast and when to use which type
Hmm well they're marked 160. In any case, they're pretty good but not worth going out of your way to accommodate.
- Thu May 25, 2023 1:42 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2524
Re: Types of Phase Contrast and when to use which type
Ah right, I meant the kyowa ones. I have seen that I miller has some of the Olympus lenses but I have never handled one, nor have I even seen a photo of the Galen Olympus turret.
- Thu May 25, 2023 12:13 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2524
Re: Types of Phase Contrast and when to use which type
The Galen phase lenses are not compatible with either the regular B&L phase or the flat field. The regular and flat field phase lenses/ diaphragms are close, except the 10x. The Galen lenses are pretty good looking plan dark medium phase achromat lenses! It is uncommon to see the proper turret, part...
- Wed May 24, 2023 7:40 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 8579
Re: How many microscopes are enough? :<)
The 1942 B&L
- Wed May 24, 2023 4:47 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 8579
Re: How many microscopes are enough? :<)
The difference in hoarding and collecting is organization and storage
- Thu May 11, 2023 9:46 pm
- Forum: Microscopes and optics
- Topic: Inverted vs Stereoscope or Compound for Pond water organisms
- Replies: 51
- Views: 30146
Re: Inverted vs Stereoscope or Compound for Pond water organisms
AN inverted scope is best used to look at things that sink to the bottom so the can press up against the glass, or things you need to access while looking at them. You can get LWD condensers with NA of .5 readily-- they are pretty big and heavy and don't always fit. I use a .4 Nikon condenser when u...
- Sat May 06, 2023 2:38 pm
- Forum: Microscopy accessories
- Topic: Amateur microscopy and professional micrometer
- Replies: 13
- Views: 3150
Re: Amateur microscopy and professional micrometer
Yeah, using a big competent microtome the 10 μm setting is for course shaping of the block, then you set it to about half of that to cut the ribbon. Thinner than 5 μm is what you might call "ultramicrotomy," but in any case even your regular microtome should do about an order of magnitude thinner (a...
- Fri May 05, 2023 12:48 pm
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 7712
Re: Epi-Fluorescence Microscopy and Deconvolution
I guess I am confused, does Photoshop have a deconvolution algorithm? I assumed that was what was being discussed as gnu image manipulation program has a genuine one and it is merely a free clone of whatever version Photoshop was at a few years ago.
- Fri May 05, 2023 12:43 pm
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 7712
Re: Epi-Fluorescence Microscopy and Deconvolution
I am not immediately familiar with Photoshop, but I assume when Berdan said he was using Photoshop deconvolution he was using some implementation of a deconvolution algorithm in Photoshop, not one of photoshops non-deconvolution algorithms. I would argue that a method that estimates a psf just from ...
- Thu May 04, 2023 1:57 pm
- Forum: Microscopes and optics
- Topic: Zeiss GFL compatible objectives?
- Replies: 17
- Views: 2332
Re: Zeiss GFL compatible objectives?
The epiplan hd objectives require a special set up, larger threads and different lighting. No problem, the others work well. If I'm not mistaken, all Zeiss objectives from that era were designed to work with compensating eyepieces, so they should be interchangeable. The neofluars are famous for.bein...
- Wed May 03, 2023 12:16 am
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 7712
Re: Epi-Fluorescence Microscopy and Deconvolution
Call me a numbskull, but I don't understand how you'd deconvolve an image without having a solid grip on how the image was convoluted in the first place. I guess that why you'd (have to) figure out the point spread function of your optical system and then feed that into a specifically designed de-c...
- Wed Apr 12, 2023 11:45 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 8579
Re: How many microscopes are enough? :<)
Gonna want a stain scope for doing your stains it gets messy and chemically so good to have a separate scope
- Fri Mar 31, 2023 5:07 am
- Forum: Microscopes and optics
- Topic: Poor man’s "Kohler" illumination
- Replies: 41
- Views: 5672
Re: Poor man’s Kohler illumination
Also, if you get a chance to read the paper I think you will find that a "poor man's Kohler" is exactly Kohler, as he had limited equipment but a good understanding of the principles and a knack for ingenious improvisation.
- Fri Mar 31, 2023 5:04 am
- Forum: Microscopes and optics
- Topic: Poor man’s "Kohler" illumination
- Replies: 41
- Views: 5672
Re: Poor man’s Kohler illumination
You can find and read Kohler's paper--its actually quite entertaining and not really overly technical. Although he did make use of a field aperture, really the essential components are a field lens and an adjustable condenser. Without these you can't really get the even illumination effect. Without ...
- Mon Mar 27, 2023 3:43 am
- Forum: Microscopes and optics
- Topic: Poor man’s "Kohler" illumination
- Replies: 41
- Views: 5672
Re: Poor man’s Kohler illumination
Kohler himself used cardboard with holes cut in them. It seems also like you could focus on the frosted glass and then simply move the condenser up about 10mm or so.
- Wed Mar 22, 2023 3:54 pm
- Forum: Microscopes and optics
- Topic: PL 25 na 0.40 PHP ROSSBACH?
- Replies: 5
- Views: 1107
Re: PL 25 na 0.40 PHP ROSSBACH?
Some of those Galen objectives are really quite nice. The condenser is tough to track down.
- Wed Mar 22, 2023 3:18 am
- Forum: Microscopes and optics
- Topic: What are the most common inverted scopes?
- Replies: 4
- Views: 968
Re: What are the most common inverted scopes?
Bausch and Lomb presented an inverted scope in the 19th century. It didn't really catch on right away and they stopped making inverted scopes until the projection scope design from mid century or the I think Photozoom a little while later. They also sold a few inverted metalloscopes now and then tha...
- Sat Mar 18, 2023 4:27 am
- Forum: Camera systems and imaging
- Topic: A potential Game-Changer
- Replies: 25
- Views: 7021
Re: A potential Game-Changer
If I ever get it working I might try to post a video of a 'tode whipping around to demonstrate the shutter system. Until then, I'm out two cameras.
- Sat Mar 18, 2023 4:24 am
- Forum: Camera systems and imaging
- Topic: A potential Game-Changer
- Replies: 25
- Views: 7021
Re: A potential Game-Changer
So, after having used the Pi HQ camera for a couple of years, I of course bought this one. I can say that it seems like a decent enough camera, but I would recommend against it. There is essentially no software to run it that I can find anywhere, just a thousand supercilious dweebs whose advice is m...
- Fri Mar 10, 2023 8:55 pm
- Forum: Microscopes and optics
- Topic: Spencer fluorite objetive
- Replies: 3
- Views: 961
Re: Spencer fluorite objetive
I think you will likely want the compensating eyepieces 167 or 168 with that one, or perhaps the planoscopic.
- Tue Mar 07, 2023 8:25 pm
- Forum: Microscopes and optics
- Topic: Are 60x objectives worth it?
- Replies: 8
- Views: 1525
Re: Are 60x objectives worth it?
Now a 60x immersion lens, that's a good lens
- Thu Mar 02, 2023 4:36 am
- Forum: Introduce yourself
- Topic: Brand new to microscopy
- Replies: 9
- Views: 2411
Re: Brand new to microscopy
That's a cool little scope there and some decent lenses. Should give good images.