MicrobeHunter.com Microscopy Forum

Dry mounts are good for low power observation. The optical reason you might want a resin or wet mount might be to maintain more homogenous optical medium when using immersion oil. Also, especially for in vertebrate skeletons, chemical processing can allow for flattening, and scopes love a flat subje...

The reduction sizes are also important to match the image projected and the size of your sensor. If you have a very small sensor and use the 1x the image will be blurry. If you have a full size sensor and use too much reduction you will get a small circle of image but the rest will be black. You can...

Well this lister thinks this is a dark field condenser and says it also might be a Leica? So maybe they don't have.it labeled oll korrect.

Well nematodes are pretty transparent so phase can help you find them or look at stuff like their stylet bulb a little better but morphological if of nematodes is p hard but phase is good for lots of stuff in soil like bacteria shapes even but you are able to peer into transparent things and see the...

There is also a reasonably flat portion of the focal length/ frequency function of the humble achromat lens around the green wavelength. Predominantly green light can make black and white photos of subjects through aachromat lenses very sharp.

Might have a copy of that one around here somewhere. I can look this weekend.

I have tried to see if the .5 lens from the regular line would work on the sz7 and it was unsuccessful. It may be that the different attachment points were introduced to prevent mixing the lenses.

The 2x sz7 is decently common, and you could probably find one for around $50 with a bit of patience

You could try white paper. It's not extremely reflective and really designed to have the Nicholas shining right one it.

It's never too bright

The wiring'll be fine, you already noted that it will pull half the wattage. There are sometimes issues when filament position is critical, but I don't think it's too sensitive with the optilume.

I seem to recall one of the diatomists here using that anti static gun to help when positioning things.

I like the b&l sz7 but you might want to stay away if you aren't a hands in kind of person as almost all of them need a little work. An sz4 is cheaper and they made a million of them. Sometimes can be had for very little. They're not the most superb imaging available but they are madly convenient. T...

I don't think Nikon ever made a cheap plan apo.

Good forceps

Yeah you oughta talk to Apochronaut about buying direct if you're contemplating laying down six or seven thou on a microscope. A little more legwork maybe but might be worth your time.

Yeah the black knobbly base. Some are Bausch and Lomb, some are Leica.

Hmm well they're marked 160. In any case, they're pretty good but not worth going out of your way to accommodate.

Ah right, I meant the kyowa ones. I have seen that I miller has some of the Olympus lenses but I have never handled one, nor have I even seen a photo of the Galen Olympus turret.

The Galen phase lenses are not compatible with either the regular B&L phase or the flat field. The regular and flat field phase lenses/ diaphragms are close, except the 10x. The Galen lenses are pretty good looking plan dark medium phase achromat lenses! It is uncommon to see the proper turret, part...

The 1942 B&L

The difference in hoarding and collecting is organization and storage

AN inverted scope is best used to look at things that sink to the bottom so the can press up against the glass, or things you need to access while looking at them. You can get LWD condensers with NA of .5 readily-- they are pretty big and heavy and don't always fit. I use a .4 Nikon condenser when u...

Yeah, using a big competent microtome the 10 μm setting is for course shaping of the block, then you set it to about half of that to cut the ribbon. Thinner than 5 μm is what you might call "ultramicrotomy," but in any case even your regular microtome should do about an order of magnitude thinner (a...

I guess I am confused, does Photoshop have a deconvolution algorithm? I assumed that was what was being discussed as gnu image manipulation program has a genuine one and it is merely a free clone of whatever version Photoshop was at a few years ago.

I am not immediately familiar with Photoshop, but I assume when Berdan said he was using Photoshop deconvolution he was using some implementation of a deconvolution algorithm in Photoshop, not one of photoshops non-deconvolution algorithms. I would argue that a method that estimates a psf just from ...

The epiplan hd objectives require a special set up, larger threads and different lighting. No problem, the others work well. If I'm not mistaken, all Zeiss objectives from that era were designed to work with compensating eyepieces, so they should be interchangeable. The neofluars are famous for.bein...

Call me a numbskull, but I don't understand how you'd deconvolve an image without having a solid grip on how the image was convoluted in the first place. I guess that why you'd (have to) figure out the point spread function of your optical system and then feed that into a specifically designed de-c...

Gonna want a stain scope for doing your stains it gets messy and chemically so good to have a separate scope

Also, if you get a chance to read the paper I think you will find that a "poor man's Kohler" is exactly Kohler, as he had limited equipment but a good understanding of the principles and a knack for ingenious improvisation.

You can find and read Kohler's paper--its actually quite entertaining and not really overly technical. Although he did make use of a field aperture, really the essential components are a field lens and an adjustable condenser. Without these you can't really get the even illumination effect. Without ...