MicrobeHunter.com Microscopy Forum

W.M. stands for "Whole Mount". The whole specimen is in mounting medium. Good only for small insects or insect parts. Put insect in alcohol to dehydrate and then into Euparal mounting medium. If this is not available, place insect into acetone for some time and then try mounting with clear nail poli...

stereo microscopes? yes, same principle.

you need a dslr adapter with optics. the image must be picked up there where the objective projects it (1cm down the tube). If not, then you need to refocus the microscope, which results in the objective not operating at the ideal distance and lower image quality. dslr adapters without optics (prime...

I would contact someone from Olympus. The phase annuli of the condenser and the phase rings have to match as well.

Watch this video, where I tested a DSLR adapter: https://youtu.be/Ju8rgeJr3bI The one with the optics is better, because it can give you mostly a parfocal image. It will pick up the image from inside the tube. The one without optics will introduce extra empty magnification and it is not parfocal, be...

Hello, Please no medical talk on this forum. I want to provide a justification for this rule. First, it is impossible to diagnose illnesses like this using microscopy. The shape of blood cells says nothing about health and any irregular or unusual shapes can be explained by artifacts. For medical ad...

Hello, This is not an easy thing. According to Olympus (I talked to them), they only provide DIC with plan Apos. The non-plan are not certified for DIC, so maybe they would still work? You need a DIC condenser and a Wollasone Prism and depending on the system you might need a separate prism for each...

Sorry for the late reply, but thank you for asking. Yes, the last issue was back in 2017 and one reason why I (temporarily?) stopped making the magazine was becasue I had somewhat of a difficulty in obtaining articles. There were several microscopists who were submitting articles on a regular basis ...

Hello! First of all, we, the microscopy community, would like to welcome new members to the forum! Please be advised, however, that this is an amateur microscopy forum and that medical advice (or medical issues) generally are not to be discussed here. One reason is, that there are many people out th...

Dear Microscopy Community, In response to some abusive posts in the past and some feuds between members, I have updated the forum rules - and I will become strict. From now on, I will not only remove offensive posts, but I will also block/remove members that attack other members on this forum. Shoul...

Dear Microscopy Enthusiasts! You probably have noticed, that this forum now looks a bit different than it used to. It was updated to the newest version, which was recommended for a variety of reasons, including security and performance. In addition I updated the PHP version (for those who are intere...

While I do not know this mountant, I suggest that you try to de-gas it somehow by applying a vacuum. Maybe air was mixed into the medium during transport (shaking) and this one might form bubbles during drying. If the medium shrinks during drying, then this can also cause bubbles. I suggest then to ...

The phase plate in the objective will reduce the resolution somewhat. But I think that there are other factors that can outweigh the loss of quality (dust, mounting medium etc).... A wrong condenser aperture setting will also reduce the resolution and I still often use it, because I get a larger dep...

Go for brightfield first. It is cheaper and once you are more experienced, you can always invest in a more expensive one later. Phase contrast is significantly more expensive. Phase contrast is nice for sure, but it is also not suitable for all specimens. If you use filters than you can get a lot ou...

Hello,
yes, it looks like a conidium:
https://en.wikipedia.org/wiki/Conidium

Oliver

Hello, It is not possible to identify bacteria based on microscopic observation alone. Totally unrelated bacteria can have the same shape and bacteria that are related might have different shapes. For identification of the bacteria, you need to do either a DNA study or a biochemical analysis of the ...

Dear Forum Members, First of all, a welcome to all new forum members, who have recently joined this forum. Many of you may already know that I am not only administrating this forum, but also creating Youtube videos. I have now started a second microscopy-related Youtube Channel and want to invite yo...

Hello, 1. Check if the condenser has a push on filter holder (and not a swing out type). The filter holder sometimes contains a blue glass filter. The patch stop goes on top of the blue glass. 2. If you do not have a filter holder at all, then make yourself a patch stop using overhead foil. Tape it ...

Hello,
Had similar lines on my slide. Try to clean the slide first before use by dry-rubbing it with a paper towel. Could be some stuff left over from the slide-manufacturing process.
greets, Oliver.

Hi,
in my setup I project it directly into the camera, but there is the problem of stray light (and thus lower contrast) if you do not have Koehler illumination. So this might be seen as another advantage for the afocal setup.
Oliver.

Hello, The NFK projection eyepieces are so-called compensating eyepieces. This means that they correct lens errors (aberrations) that are still present from the objective. The NFK eyepieces are designed to project the image diretly on flat photographic film or sensor. They are not designed for afoca...

Hello Cfloridanus! I am the administrator of this forum and also want to welcome you personally - because I also studied at the University of Vienna (Microbiology). I now live in Upper Austria and hope that you enjoy your stay here in Austria. At this point I also want to "advertise" my Youtube Chan...

Oblique illumination - several points: 1. Not all specimens will produce a good result. To test it, I would try to make leaf-impressions with glue and then observe the glue, because this worked fine for me. White glue on bottom side of leaf, let it dry, peel it off, observe it. Stomata should be vis...

Hello,

I once wrote an article about it, but will also do a video soon:
http://www.microbehunter.com/oblique-illumination/

Oliver

Hello forum members!



Link to the new youtube channel (please subscribe):
https://www.youtube.com/channel/UCDpKY8 ... BPNzNsbLug

greetings, Oliver

Hello, first of all, great pictures. Microscopy alone often does not provide enough information to identify something down to the species level. And: due to the large number of yet unclassified microorganisms, it is not even unlikely that you are dealing with a new species. The typical spiral shape ...

I was just wondering if the forum still works, because there are few posts for some days. (I will remove this thread anyway)
Oliver.

this is a test post

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