Search found 58 matches

by admin
Thu Jan 21, 2021 4:30 pm
Forum: Specimens, samples and slides
Topic: How to make Insect w.m. Microscope Slide
Replies: 6
Views: 800

Re: How to make Insect w.m. Microscope Slide

W.M. stands for "Whole Mount". The whole specimen is in mounting medium. Good only for small insects or insect parts. Put insect in alcohol to dehydrate and then into Euparal mounting medium. If this is not available, place insect into acetone for some time and then try mounting with clear nail poli...
by admin
Wed Jan 13, 2021 6:02 pm
Forum: Camera systems and imaging
Topic: DSLR photography
Replies: 5
Views: 845

Re: DSLR photography

stereo microscopes? yes, same principle.
by admin
Tue Jan 12, 2021 8:28 pm
Forum: Camera systems and imaging
Topic: DSLR photography
Replies: 5
Views: 845

Re: DSLR photography

you need a dslr adapter with optics. the image must be picked up there where the objective projects it (1cm down the tube). If not, then you need to refocus the microscope, which results in the objective not operating at the ideal distance and lower image quality. dslr adapters without optics (prime...
by admin
Tue Jan 12, 2021 8:16 pm
Forum: Beginner's corner
Topic: phase contrast microscopy, the right condenser
Replies: 2
Views: 830

Re: phase contrast microscopy, the right condenser

I would contact someone from Olympus. The phase annuli of the condenser and the phase rings have to match as well.
by admin
Tue Jan 12, 2021 7:52 pm
Forum: Beginner's corner
Topic: Canon EOS DSLR to 23.2mm adapter
Replies: 15
Views: 1953

Re: Canon EOS DSLR to 23.2mm adapter

Watch this video, where I tested a DSLR adapter: https://youtu.be/Ju8rgeJr3bI The one with the optics is better, because it can give you mostly a parfocal image. It will pick up the image from inside the tube. The one without optics will introduce extra empty magnification and it is not parfocal, be...
by admin
Sat Jun 06, 2020 6:34 am
Forum: Pictures and Videos
Topic: Identification Help
Replies: 4
Views: 652

Re: Identification Help

Hello, Please no medical talk on this forum. I want to provide a justification for this rule. First, it is impossible to diagnose illnesses like this using microscopy. The shape of blood cells says nothing about health and any irregular or unusual shapes can be explained by artifacts. For medical ad...
by admin
Thu Apr 23, 2020 10:45 pm
Forum: Illumination Techniques
Topic: DIC prism purchasing
Replies: 7
Views: 1664

Re: DIC prism purchasing

Hello, This is not an easy thing. According to Olympus (I talked to them), they only provide DIC with plan Apos. The non-plan are not certified for DIC, so maybe they would still work? You need a DIC condenser and a Wollasone Prism and depending on the system you might need a separate prism for each...
by admin
Fri Apr 17, 2020 9:43 am
Forum: About the website, the magazine and this forum
Topic: What happened to the Microbe Hunter magazine?
Replies: 9
Views: 8030

Re: What happened to the Microbe Hunter magazine?

Sorry for the late reply, but thank you for asking. Yes, the last issue was back in 2017 and one reason why I (temporarily?) stopped making the magazine was becasue I had somewhat of a difficulty in obtaining articles. There were several microscopists who were submitting articles on a regular basis ...
by admin
Thu Apr 02, 2020 7:24 pm
Forum: Beginner's corner
Topic: ID of pathogen of minor skin infection.
Replies: 2
Views: 866

Re: ID of pathogen of minor skin infection.

Hello! First of all, we, the microscopy community, would like to welcome new members to the forum! Please be advised, however, that this is an amateur microscopy forum and that medical advice (or medical issues) generally are not to be discussed here. One reason is, that there are many people out th...
by admin
Mon Nov 25, 2019 11:39 pm
Forum: Important announcements
Topic: Updated Forum Rules
Replies: 4
Views: 11446

Updated Forum Rules

Dear Microscopy Community, In response to some abusive posts in the past and some feuds between members, I have updated the forum rules - and I will become strict. From now on, I will not only remove offensive posts, but I will also block/remove members that attack other members on this forum. Shoul...
by admin
Tue Sep 03, 2019 9:09 pm
Forum: Important announcements
Topic: Forum Update
Replies: 7
Views: 5929

Forum Update

Dear Microscopy Enthusiasts! You probably have noticed, that this forum now looks a bit different than it used to. It was updated to the newest version, which was recommended for a variety of reasons, including security and performance. In addition I updated the PHP version (for those who are intere...
by admin
Tue Jul 23, 2019 8:52 pm
Forum: Specimens, samples and slides
Topic: Bubbles in drying mountant
Replies: 1
Views: 1941

Re: Bubbles in drying mountant

While I do not know this mountant, I suggest that you try to de-gas it somehow by applying a vacuum. Maybe air was mixed into the medium during transport (shaking) and this one might form bubbles during drying. If the medium shrinks during drying, then this can also cause bubbles. I suggest then to ...
by admin
Tue Jul 23, 2019 8:47 pm
Forum: Beginner's corner
Topic: Phase contrast objectives
Replies: 2
Views: 1842

Re: Phase contrast objectives

The phase plate in the objective will reduce the resolution somewhat. But I think that there are other factors that can outweigh the loss of quality (dust, mounting medium etc).... A wrong condenser aperture setting will also reduce the resolution and I still often use it, because I get a larger dep...
by admin
Tue Jul 23, 2019 8:43 pm
Forum: Beginner's corner
Topic: Phase contrast vs. Brightfield
Replies: 1
Views: 1403

Re: Phase contrast vs. Brightfield

Go for brightfield first. It is cheaper and once you are more experienced, you can always invest in a more expensive one later. Phase contrast is significantly more expensive. Phase contrast is nice for sure, but it is also not suitable for all specimens. If you use filters than you can get a lot ou...
by admin
Sun Jul 14, 2019 5:56 pm
Forum: Identification help
Topic: Is this a mould spore?
Replies: 2
Views: 2561

Re: Is this a mould spore?

Hello,
yes, it looks like a conidium:
https://en.wikipedia.org/wiki/Conidium

Oliver
by admin
Wed Jul 03, 2019 7:21 pm
Forum: Pictures and Videos
Topic: Help Identify Gram Negative Cocci Please
Replies: 9
Views: 2866

Re: Help Identify Gram Negative Cocci Please

Hello, It is not possible to identify bacteria based on microscopic observation alone. Totally unrelated bacteria can have the same shape and bacteria that are related might have different shapes. For identification of the bacteria, you need to do either a DNA study or a biochemical analysis of the ...
by admin
Wed Jun 05, 2019 5:28 pm
Forum: Important announcements
Topic: New Youtube Channel
Replies: 10
Views: 10553

New Youtube Channel

Dear Forum Members, First of all, a welcome to all new forum members, who have recently joined this forum. Many of you may already know that I am not only administrating this forum, but also creating Youtube videos. I have now started a second microscopy-related Youtube Channel and want to invite yo...
by admin
Wed May 22, 2019 7:50 pm
Forum: Illumination Techniques
Topic: Darkfield using Olympus BH2/BHS
Replies: 5
Views: 5257

Re: Darkfield using Olympus BH2/BHS

Hello, 1. Check if the condenser has a push on filter holder (and not a swing out type). The filter holder sometimes contains a blue glass filter. The patch stop goes on top of the blue glass. 2. If you do not have a filter holder at all, then make yourself a patch stop using overhead foil. Tape it ...
by admin
Sun Apr 21, 2019 8:33 pm
Forum: Identification help
Topic: Can't find an answer on what these black lines could be?
Replies: 4
Views: 3857

Re: Can't find an answer on what these black lines could be?

Hello,
Had similar lines on my slide. Try to clean the slide first before use by dry-rubbing it with a paper towel. Could be some stuff left over from the slide-manufacturing process.
greets, Oliver.
by admin
Wed Mar 13, 2019 9:06 pm
Forum: Introduce yourself
Topic: Olympus BH2 - AFocal vs projection eyepiece?
Replies: 3
Views: 3025

Re: Olympus BH2 - AFocal vs projection eyepiece?

Hi,
in my setup I project it directly into the camera, but there is the problem of stray light (and thus lower contrast) if you do not have Koehler illumination. So this might be seen as another advantage for the afocal setup.
Oliver.
by admin
Tue Mar 12, 2019 11:13 pm
Forum: Introduce yourself
Topic: Olympus BH2 - AFocal vs projection eyepiece?
Replies: 3
Views: 3025

Re: Olympus BH2 - AFocal vs projection eyepiece?

Hello, The NFK projection eyepieces are so-called compensating eyepieces. This means that they correct lens errors (aberrations) that are still present from the objective. The NFK eyepieces are designed to project the image diretly on flat photographic film or sensor. They are not designed for afoca...
by admin
Tue Jan 29, 2019 9:45 pm
Forum: Introduce yourself
Topic: Hello, from Vienna, Austria -(Technically Mobile, Alabama USA)
Replies: 10
Views: 3691

Re: Hello, from Vienna, Austria -(Technically Mobile, Alabama USA)

Hello Cfloridanus! I am the administrator of this forum and also want to welcome you personally - because I also studied at the University of Vienna (Microbiology). I now live in Upper Austria and hope that you enjoy your stay here in Austria. At this point I also want to "advertise" my Youtube Chan...
by admin
Mon Aug 13, 2018 9:37 pm
Forum: Illumination Techniques
Topic: How is this not DIC? Oblique illumination? HOW?
Replies: 10
Views: 5927

Re: How is this not DIC? Oblique illumination? HOW?

Oblique illumination - several points: 1. Not all specimens will produce a good result. To test it, I would try to make leaf-impressions with glue and then observe the glue, because this worked fine for me. White glue on bottom side of leaf, let it dry, peel it off, observe it. Stomata should be vis...
by admin
Sun Aug 12, 2018 6:02 am
Forum: Illumination Techniques
Topic: How is this not DIC? Oblique illumination? HOW?
Replies: 10
Views: 5927

Re: How is this not DIC? Oblique illumination? HOW?

Hello,

I once wrote an article about it, but will also do a video soon:
http://www.microbehunter.com/oblique-illumination/

Oliver
by admin
Sun Jan 14, 2018 8:54 pm
Forum: Important announcements
Topic: Announcement
Replies: 7
Views: 12840

Announcement

Hello forum members!



Link to the new youtube channel (please subscribe):
https://www.youtube.com/channel/UCDpKY8 ... BPNzNsbLug

greetings, Oliver
by admin
Mon May 29, 2017 5:50 am
Forum: Pictures and Videos
Topic: Questions from our town's old reservoir
Replies: 6
Views: 2265

Re: Questions from our town's old reservoir

Hello, first of all, great pictures. Microscopy alone often does not provide enough information to identify something down to the species level. And: due to the large number of yet unclassified microorganisms, it is not even unlikely that you are dealing with a new species. The typical spiral shape ...
by admin
Mon May 15, 2017 4:54 am
Forum: Sandbox
Topic: test post
Replies: 3
Views: 5802

Re: test post

I was just wondering if the forum still works, because there are few posts for some days. (I will remove this thread anyway)
Oliver.
by admin
Sat May 13, 2017 8:29 pm
Forum: Sandbox
Topic: test post
Replies: 3
Views: 5802

test post

this is a test post
by admin
Fri Nov 25, 2016 10:51 pm
Forum: Important announcements
Topic: Forum Rules!
Replies: 0
Views: 5714

Forum Rules!

These rules are occasionally updated, therefore if is the obligation of every forum user to check this post regularly. Rules: 1. No bad language of any kind. 2. No personal attacks of any kind. This includes ridicule, condescending remarks or threats. 3. No criticism of products that you are not sat...
by admin
Thu Nov 24, 2016 7:42 pm
Forum: Sandbox
Topic: easyzzom test
Replies: 11
Views: 9320

easyzzom test