MicrobeHunter.com Microscopy Forum

Hi, this forum is packed with very knowledgeable folk re equipment. Yours is an often-asked question of course, and a look through past posts - many very recent or active, will get you off to an interesting and useful start. My one piece of advice is to buy nothing until you've spent a little time w...

Urk - those SEM images are quite stunning - the detail & complexity is extraordinary. BUT, all you diatom-botherers must not let this discourage you, rather let it enliven, augment and increase your enjoyment - every session of observation has the chance of revealing that known but as-yet unseen ext...

Nice video - very crisp and some good detail.

Aha! It's a rudimentary hand-section cutter - for soft stems of small diameter. Push a small soft stem piece through one of the holes, from below. Set it to just be sticking up above the hole's side by about 1mm then turn the blade-holding part to cut a section. Place under your 'scope - in a drop o...

You may try a weak glycerin/water mix - perhaps 1:10 (v/v) - but with a glycerin content that is enough to cover the sample if used on it's own. The idea being to put the leaves into the mixture then allow the water content to slowly evaporate - thus gradually increasing the concentration of glyceri...

Urk - I see what you mean - can't open it. AVS doc converter says it's an invalid or broken file also.... I'll have a closer look at it and get back.

Once downloaded it'll open with Chrome?

Hi 75' Here's a link to the pdf in my shared g-drive... When you click on the link the message will tell you that the document can't be viewed - but it CAN be downloaded - look top RHS for the download icon on this dark message screen - it'll download to your machine for you - I hope... See this scr...

A really nice set of images!

Hi Apo' and Hobby' - I'd agree - definite differences - perhaps made a tad murky by jpg, but they exist. Good enough though for the purchase of a phase objective whilst waiting for a non-phase (assuming the use of phase is also relevant of course). What we need is some better images for comparison -...

ARRGGHHHH!!! Goomba - I just accidentally 'reported' your post - oops - I meant to use the quote button but accidentally used the exclamation-mark (report-post button...) - apologies :oops: :oops: I'll PP these images also to see just how close they can get to each other. The posted 'faithful' versi...

Hi, I just had a quick look at a couple of images for a rough comparison. Comparing an Achromat Ph2 40x Olympus infinity objective to an Olympus PlanC N UIS2 'ordinary' BF objective, as image, WS_phase nosepiece (3).jpg I also keep a phase and BF nosepiece, WS_phase nosepiece (2).jpg The subject isn...

Hi, I'd say you have a cracking start with the Olympus!

Hi, I posted a video on Youtube of the process myself some time ago now -

Microsoft ICE is superb and fast for stitching.....

That's why it's anatomy isn't the unifacial stem type - it's a petiole ('leaf stalk') - a little like a stem that hasn't fully 'wrapped into a cylinder' as it were - hence the lack of a ring of vascular bundles etc. In fact Bob's also looks like a petiole rather than a stem section - a lovely fresh ...

Hi Marco, a nice looking 'scope! Perhaps a variable neutral -density filter (as screwed onto the fron of a camera) on top of the light port will help. With this you'll be able to to keep the halogen at it's whitest full-ish setting and control light intensity with a turn of the rotating VND filter.....

I think you're right 75' - I definitely see different effects with the various (Weighted-Average, Depth-Map, Pyramid) options in Helicon Focus - especially I suspect with the very often 'soft' images of obscure detail within a mounted-section rather than optimally at the coverslip's underside.

Hi 75', I've just started using Bridge/Photoshop (and Lightroom-Classic for fast batch edits) and have found the stacking to be pretty good in PS also. Is there a way to know what type of stacking PS is performing, or even maybe to alter parameters do you know? Perhaps the absence of parameters poin...

MichaelG. wrote: ↑

Sun Feb 09, 2020 10:21 am

Fabulous image !

At this resolution I can’t see any evidence of the stitching
... is it really that good ?

MichaelG.

Ditto Michael!
It really is - when combined with 75's consummate skill with this work - fantastic inspiration and goal for others.

Hi zero, I use sewing-machine oil on my 'tome's (Shandon) advance threads. I use mine quite often but it only seems to require oil about once every couple of years if that!

Hi, the 170 TL NPL and PL are for the SM-Lux, The EF are, as far as I'm aware, for the 160TL 'scopes.

Personally I'd choose the 25x in either set - a very handy magnification that I always include on my 'scopes (or a near-by 20x...).
The SM-Lux is a lovely 'scope in use in my experience.

Simply Stunning - almost looks like a Japanese fan. A
beautiful image, superbly executed - your standards always leave me astonished 75'.

Hi Jan, a tricky manoeuvre for sure! I'd try to secure or even seal the coverslip before going to oil. Perhaps a couple of dabs of quick-setting 'stuff' at a few points around the coverslip/slide edges. I wonder if some small dabs of wax may be able to secure the coverslip and remain unaffected by t...

This looks like the Nikon F equivalent to the Canon?

Hi, for my BX50's phototube (as mentioned, it 'comes with an Olympus connection') I simply fitted an Olympus--->Canon adapter-ring (cost under £10) and all's perfectly parfocal. This is an infinity system with the 'PE' projection eyepiece rather than the corrected NFK version. These are the pieces I...

Hi, looks very much like a pollen grain, possibly from the Lily family....

A very nice image and effect/s indeed. As said - very dramatic.
You've clearly a great technique old chap, nicely-done!

Volpi made fiber goosenecks that clip on to at least some leica focus mounts, though this one is for the older style rack and pinion https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F163986506082 I just clamped one onto the back of my Olympus stand with literal hardwa...