Search found 3553 matches

by mrsonchus
Sun Oct 20, 2019 10:35 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 136

Re: Marine Alga

75', this may be a stupid question, but what is needed for a start with DIC, re my BX40?
by mrsonchus
Sun Oct 20, 2019 10:18 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 136

Re: Marine Alga

What an image 75'!

Such detail, this DIC is pretty impressive! Very nicely-done old chap.
by mrsonchus
Sat Oct 19, 2019 11:18 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 114

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Hi Wes, here are a few images of longitudinal sections (permanently-mounted & stained) of Sonchus (AKA 'milkweed' I think) stems, as seen with polarisation and the full wave plate. Each image is at a different orientation and shows as in your excellent images, fiber orientation variance relative to ...
by mrsonchus
Sat Oct 19, 2019 8:16 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 404

Re: NO LONGER WANTED - Olympus nosepiece adapter

75RR wrote:
Sat Oct 19, 2019 7:37 pm
Excellent news! Congratulations.
Thanks 75' - I'm so pleased I'm almost purring this evening!

You look like a fellow that needs a BX40 to me my friend! :D :D ;)
by mrsonchus
Sat Oct 19, 2019 8:15 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 404

Re: NO LONGER WANTED - Olympus nosepiece adapter

EXACTLY my friend - that's the part I have been looking for to enable the major upgrade of my BX40. I found another BX40 with one fitted, and bought it. Now I'm selling it on and my BX40 has a lovely 6-way removable nosepiece thanks to the adapter! The part name is 'READB' - not easy to find, not ea...
by mrsonchus
Sat Oct 19, 2019 7:52 pm
Forum: For forum members who want to buy and sell equipment
Topic: FOR SALE - OLYMPUS BX40 COMPLETE WITH UIS2 OBJECTIVES
Replies: 0
Views: 43

FOR SALE - OLYMPUS BX40 COMPLETE WITH UIS2 OBJECTIVES

Hi all, I have a spare Olympus BX40 that I'm offering for sale. I CAN OFFER A SIGNIFICANT DISCOUNT FROM MY E-BAY PRICE TO FORUM MEMBERS IN THE U.K. WITH A DIRECT SALE AS E-BAY FEES WILL NOT BE CHARGED This one is available as I bought 2 to effectively make one, that I now use, and have this one to s...
by mrsonchus
Sat Oct 19, 2019 7:26 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 404

Re: NO LONGER WANTED - Olympus nosepiece adapter

75! Well, erm, the thing-is my friend, I saw then bought another BX40, for a very affordable price that will allow me to sell it on at a sensible price... The 'extra' BX40 arrived safely and has all the parts I'm after - and more! For starters, it had, past-tense :D , the adapter to enable the use o...
by mrsonchus
Sat Oct 19, 2019 11:16 am
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 404

Re: NO LONGER WANTED - Olympus nosepiece adapter

Hi Bob, yes there's a tiny 'piece of dovetail-profile' as it were, that the set-screw pushed against. This then, being a nice fit for the edge of the adapter, by virtue of it's matching profile, completes the fit. This image shows the tiny piece in-situ, https://lh3.googleusercontent.com/0f71RZ7EENB...
by mrsonchus
Sat Oct 19, 2019 9:01 am
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 114

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Lovely and as said informative post Wes! These spirals are in fact the lignified secondary cell-walls from the Xylem-vessels of a plant. The primary cell-wall (which would have covered the spirals like a sleeve as it were) are gone and just the reinforcing spirals of lignin remain. The reason they'r...
by mrsonchus
Thu Oct 17, 2019 10:46 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 404

NO LONGER WANTED - Olympus nosepiece adapter

Hi all,

this request is now over - I have found the parts I need!
by mrsonchus
Thu Oct 17, 2019 10:45 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED Olympus intermediate polariser
Replies: 31
Views: 889

NO LONGER NEEDED Olympus intermediate polariser

Hi all,

this request is now over - I have found the polarising parts I need!
by mrsonchus
Tue Oct 15, 2019 11:07 pm
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 125

Re: Mixed FN objectives on same head?

Hi Pete, I see what you mean.. I also have (it arrived today) an aplanat/achromat 1.4 n.a. condenser that doesn't show this effect with either of the 60x objectives of n.a. 0.8 and 0.9. What I have subsequently found after some testing and comparisons this afternoon is that the apparent resolution a...
by mrsonchus
Tue Oct 15, 2019 8:37 am
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 125

Re: Mixed FN objectives on same head?

Hi Michael, I don't know but the super and norma widefield eyepieces look the same size.. What would be the reasoning for this? It's an interesting question though. one I may contact Olympus about? The cheapo Chinese 60x 0.8 n.a. FN22 objective I have has exctly the same image in terms of brightness...
by mrsonchus
Tue Oct 15, 2019 6:20 am
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 125

Re: Mixed FN objectives on same head?

Thanks Pete. There's a difference though when setting the condenser's n.a. to that of the objective. When the rear focal plane of the objective is viewed through a phase telescope the condenser's iris is correctly the width of the view (i.e. the condenser's n.a. matches that of the objective at 0.9)...
by mrsonchus
Mon Oct 14, 2019 10:53 pm
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 125

Mixed FN objectives on same head?

Hi all,a question that intrigues me. Microscope heads for the olympus BX infinity range come in a 'normal widefield' version, with 22mm FN eyepieces and 22mm FN objectives below. There is also a 'super widefiled' head with 26.5 FN eyepieces (SWH10x-H for example) and 26.5mm FN objectives below. Ques...
by mrsonchus
Mon Oct 14, 2019 4:01 pm
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 99
Views: 17684

Re: Pollen. There's lots of it about.

What a beautiful little plant! I like the hidden pollen-grains too.
Nicely-done.
by mrsonchus
Sat Oct 12, 2019 10:13 pm
Forum: My microscope
Topic: DIY DIC Effect on Darkfield.... unreal
Replies: 6
Views: 586

Re: DIY DIC Effect on Darkfield.... unreal

Hmm, there's a touch of oblique there for sure, but DIC - I don't think so. Pretty nice to experiment though, keep at it as these little surprises are by no means rare with microscopy.... By the way, your microscope looks to be a nice instrument also. Keep exploring, experimenting and sharing - you'...
by mrsonchus
Sat Oct 12, 2019 8:42 am
Forum: Beginner's corner
Topic: Fusing glass slides
Replies: 23
Views: 840

Re: Fusing glass slides

I don't suppose some-sort of glass cuvette, perhaps even 'sawn-off', would be any use?
by mrsonchus
Sat Oct 12, 2019 8:34 am
Forum: Beginner's corner
Topic: Newbie with information overload looking for first microscope
Replies: 22
Views: 919

Re: Newbie with information overload looking for first microscope

What a lovely-looking 'scope that is!
I must agree with your choice of teaching-head, perfect for the kids.

You're all in for a real treat, great to see microscopy being explored together, congratulations! :D :D
by mrsonchus
Thu Oct 10, 2019 10:54 pm
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 939

Re: Trinocular Modification for an Olympus CX31?

I agree completely Gene, it's always just a matter of time I find.
I like the look of the stereo-zoom trinoc, but way-above my budget I'm afraid old chap. If I hear of any interest I'll surely let you know.
by mrsonchus
Thu Oct 10, 2019 8:19 pm
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 939

Re: Trinocular Modification for an Olympus CX31?

That sounds about right Gene. The choice of the side-port intermediate or the trinocular.

Incidentally, did you find a supplier for the U-TRU, as I'm looking for one too?
by mrsonchus
Thu Oct 10, 2019 5:52 pm
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 939

Re: Trinocular Modification for an Olympus CX31?

Hi Gene, I've seen the -1, -2 etc additions to the end of other Olympus part numbers such as the ergonomic heads, and can't find any definitive info re this..... The super-widefield head is for use with the super-widefield objectives, i.e. those marked with a 26.6 mm fild number on the barrel. The 2...
by mrsonchus
Thu Oct 10, 2019 9:08 am
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 939

Re: Trinocular Modification for an Olympus CX31?

Hi there, will this trinocular-head fit your 'scope - the price is good and I've bought from this chap and know him to be reliable.
Link to trinocular head
by mrsonchus
Tue Oct 08, 2019 12:34 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 2941

Re: Home made (Very Basic) Microtome

Sounds-good!

I'll have a visit later, maybe even join-up.
I have to take the dog to the vet' soon..... bad teeth and partial deafness, the dog that is, not me! :D

Thanks agin Bob, I'll let you know how I get on. :)
by mrsonchus
Tue Oct 08, 2019 12:10 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 2941

Re: Home made (Very Basic) Microtome

Great link Bob - some great examples there! There's nothing more colourful than a hand-section! Some truly beautiful examples, and I love the labelling and interest.

I may have to have a look at that forum. What's the German for 'Hello'! :D

Many thanks Bob. A superb lead.
by mrsonchus
Tue Oct 08, 2019 10:15 am
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 2941

Re: Home made (Very Basic) Microtome

Plant-section posts! Have a look over my posts -- over 3k posts re plants! :D :D Hand-sectioned plants, May 2017, see my post. Some great results are possible with hand-sectioning, even without a hand-microtome. I take mine without using a microtome, just the 'back of a fingernail' does the job. htt...
by mrsonchus
Sat Oct 05, 2019 12:20 am
Forum: Pictures and Videos
Topic: Festuca Grass Hand Sections
Replies: 23
Views: 704

Re: Festuca Grass Hand Sections

Hi Bob - an interesting source for the limones!
Yes Histoclear in the lab does smell a bit of oranges, but not as much as the poor cleaner that has to spread the stuff all-over labels etc!
I can imagine that would be a bit of a horror!

Thanks for the idea, I'll see what I can find-out.
by mrsonchus
Thu Oct 03, 2019 3:10 pm
Forum: Pictures and Videos
Topic: Festuca Grass Hand Sections
Replies: 23
Views: 704

Re: Festuca Grass Hand Sections

Hi all, I ran a few - in fact I'm very low now on slides in wax ready to mount, slides of TS of the monocotyledonous garden-plant 'Solomon's seals' through the Wacker-inspired stains that I had mixed back in February I think it was. I quickly tried a few variations and in particular like the way the...
by mrsonchus
Wed Oct 02, 2019 9:16 pm
Forum: Pictures and Videos
Topic: Festuca Grass Hand Sections
Replies: 23
Views: 704

Re: Festuca Grass Hand Sections

Hi Bob - great links - I'll have a proper look over those! Interesting to immediately see that a proposed replacement for the acridine-red is Rhodamine(B)! Of course, reading further down it was now I remember the W-ASim II stain that I read of in an article - which I have somewhere. The stains was ...