MicrobeHunter.com Microscopy Forum

Looks like an Euglenoid to me. The type of movement, known as metaboly is characteristic for this group. A flexible protein layer, the pellicle, envelopes the whole cell and its what gives this organisms that extreme flexibility and resilience.

Nice video!

Does anyone have PDFs (or printed copies) of the 'Variable Amplitude Contrast' (VAC) articles that were published on the Balsam Post: Strange's Variable Amplitude Contrast, Balsam Post 91.12, Brian Wilkinson (2011) VAC Applications in Pond Life Microscopy, Balsam Post 100.22, B Wilkinson & J Wright ...

The problem with using a microarray approach is that one of the key goals is to reduce sample prep -- this means we are not culturing the colony on any growth media I don't think you'd need to go as far as culturing, though. There would definitely be a sample prep step, but so for any FISH like app...

OK, what you want is a modular set of probes each of which hybridizes to specific regions of a single 16s rRNA so that you'd need to know the combination of probes to determine the species in a FISH like approach. I suppose that's one way to go, and PALM/super-resolution might help on that but that'...

Nice! Thanks for sharing them. I recently designed a couple of custom darkfield/oblique filters with FreeCAD, but I'll try some of your designs too. The customizer/openscad source is a nice touch; will let people adapt the designs to work with a broad range of microscopes.

Out of curiosity, would you care to elaborate more about what you mean by this: The application I am thinking of is a computer vision / imaging based alternative to 16S ribosomal RNA sequencing for where the individual strains could be identified from images Taxonomy based on 16S rRNA relies on chan...

This is a crop of a photo, about 1/3 of the field of view in the original, of an Euglena roaming in 3mm of water inside a plastic petri dish that I recently took using the Invertoscope. Video of the Euglena engaged in full metaboly looks better than this slightly out of focus photo. Total magnificat...

This is a brochure of a rebranded LOMO Invertoscope; the original model was named BIOLAM P-1. Will give you an idea of how complete your's is. Judging by the photo you have all the phase plates and the polarized light analyzer (a-filter tray). If it has the objectives then you might be all set to g...

If you have access to a 3D printer and 1.5ml tubes this might be of use: dremelfuge . You can use it with a regular drill. Just be aware that running it at full tilt it might 1) generate more than 50kG's of centrifugal force and 2) in the event of a failure plastic pieces flying around can cause inj...

Just found on the web a brochure from a re-branded LOMO BIOLAM P-1 inverted microscope here . It shows that indeed DIC was possible for 10x and 20x using 2 separate filter/prism sliders with this microscope. The bigger slider, shown in the photos in previous posts, has a slith and a polarized filter...

Thanks Dmi3n and Michael. I got Pluta's paper, will be an interesting read.

Thanks for the info Bob. I've found some references to that slit condenser from the PZO in the forums.

Regards,
Jmp

Hi Dmi3n, Seems I can't reply via PM (registered recently, can receive but not send PM). Any way, thanks for the info, and yes, please send me the PDF that you have for the Biolam P-1. The model sold in the US specifically mentioned DIC as an option for it. Then there's this: https://i.ebayimg.com/i...

Hi Dmi3n, My first microscope was one of those ubiquitous BIOLAMs, which I still have and use. Recently I aquired a few more LOMO models that I'm restoring: a Metam-IQ , a Laboroscope and an Invertoscope (I'm quite fond of this last one). I've been keeping an eye on Ebay but some parts are a bit har...