MicrobeHunter.com Microscopy Forum

The best demonstration of image formation by a lens that I've ever seen in youtube:



Its insanely cool that the author of the video etched on chromium coated glass the patterns that he used for all the experiments!

Just thought others here might enjoy this video too.

I recently found out that a PAXCAM, modified as described in previous posts, works with Micro-Manager 2.0 (april version or newer, better yet, latest nightly bluild) using the bundled Lumenera driver adapter. There's a catch though, Lumenera's Infinity analyze (v7.1.1 or later) must also be installe...

Those appear to be fungal zoospores within a zoosporangium. Perhaps Saprolegnia?

As far as I know, WINE does not have a USB emulation layer. You'd need to use a virtual machine (KVM, VMWARE or VirtualBox) if you want to run Windows software in a Linux host and have access to USB devices.

I've had some success using a single Sanderson prism for DYI DIC. Here´s a diatom lineup, from one of those Victorian slide makers, with a 10x LOMO objective: DSC_3854_small.JPG This is the result of translating the prism along its shorter edge (left to right across the interference pattern, see the...

You are on the right path. Assuming that you have the same model that I have (PX-CM) the bytes from the original EEPROM are: address|content 0|0xC0 1|0x54 2|0x53 3|0x17 4|0x80 5|0x00 6|0x00 7|0x00 That's vendor_id=0x5354, product_id=0x8017 with device id and configuration bytes all equal to 0x00. Se...

essence25 wrote: ↑

Mon Oct 17, 2022 4:48 pm

Thank you for letting me know. Updated! Send me a PM...

PM sent.

I found these LOMO? or whatever DIC prisms, the upoer bigger one has a circular glass and a secondary slit with another glass behind it. The lower has a clear squarish prism glued at the edges. Can I do a anything with these to obtain some form of DIC? b664492e0435492aa3988fc70a753490 The link that...

Some thoughts about this. First, I second MicroBob's suggestion that perhaps the sleeves were swapped. I have a couple of Lomo 40x objectives, one for use with and one for use without coverslip: 20220330_170627_small.jpg I was puzzled by the poor performance of the coverslip version, until I got aro...

Are you trying to do it with like normal prism sliders? I think that would be difficult - which is why Rathi used the open frame design. The sliders for this microscope are relatively large. The smallest one is l=80mm by w=30mm, with the constraint being on the width. Two stacked polycarbonate pris...

Yes, it be good to know if that's the case. I've postponed work on this project for several reasons, one being that I need to re-design the prism sliders. My current 3D printed sliders did work for some tests, but they deform even when a small bending force is applied to the prism... and then don't ...

All that is needed is to place a piece of correctly oriented, unstrained polycarbonate in front of the condenser prism (between the condenser prism and the condenser lens) and the fringe patterns are shifted to allow proper adjustment. Such a simple answer to what seemed an intractable problem befo...

Hi Louise, Is difficult to see in this picture: https://www.microbehunter.com/microscopy-forum/download/file.php?id=24397 Are the short edges of the prism touching the sides of the frame? What about the prism edge and the bottom of the frame? From that photo it looks like there is uneven bending, as...

Very nice video!

Ah, ok jmp, thanks. Sounds like I'm trying too hard. I did post one pic of the two prisms together - 'Prism A+B'. Sorry, I didn't see the Prism A+B picture before. Though now that I've seen it, it appears to be the same photo as Prism B, right? I think I need to go back a step and try again. Also I...

Hi Louise, Note that for a single prism the interference pattern that I obtain within crossed polarizers is asymmetric, without a dark fringe band in the center: https://www.microbehunter.com/microscopy-forum/download/file.php?id=24232 The interference pattern from above is similar to the one at the...

I haven't read up on the Sanderson prism theory, and skimmed parts of this thread, so I may be missing something obvious here, but do you really need to measure and replicate the exact same divergence angles as those used in the paper? Replicating the divergence angles from the paper is, in my case...

Louise, I agree with you, the divergence angle equation is not the problem. I am clearly plugging in the wrong values for the variables in my attempt to replicate their angle vs screw displacement plot, yet I've not been able to put my finger on what exactly I'm doing wrong... it should have been pr...

Some images of the setup I've been using for testing. The individual parts: 20210602_205101.jpg left to wright, top to bottom: filter tray with a polarizer, sanderson prism slider #1 (for the back focal plane), rubber bands, analyzer, sanderson prism slider #2 (between light source and condenser). B...

The contraption that I used to obtain the image of that interference pattern is extremely simple, and it did not involve the microscope or any complex optic setup: literally the prism sliders were directly stacked on top of each other, flanked by cross polarized filters. Just imagine two of these: h...

Built the second prism and slider. The interference pattern between crossed polarizers from the two prisms, stacked and aligned on their main prism axes, look like this now: two_prism_interference_pattern.jpg A bit wavy, the 2 screws were not pushing evenly on the prisms, but it approximates the pat...

To clarify, the problem is not that the angles are negative but that the results that I get from that expression do not agree with the displacement-to-angle relationship depicted in the figure from their paper, unless I make the changes that I mentioned. I might have missed something though, its not...

(Continued) In the end I built a crude 3D printed slider to accommodate a prism of 70x16x2.38mm: slider_prototype.jpg It was quite interesting to see how the interference pattern changed as the bending force on the prism increased, when the prism was hold between a pair of crossed polar filters. Her...

I've been experimenting with Sanderson prisms too, after going over the paper from Rathi et. al. I believe there is an error in the expression that gives the divergence angle: divergence_function.jpg The problem is that with r and l defined as in their figure (Fig. 1): prism_schematic.jpg the expres...

Lactophenol coupled with methylene blue (cotton blue) seems to be the classic mounting medium used in mycology. Not sure if it can be considered a permanent medium though. Here's a page with some info about it: Lactophenol Cotton Blue (LPCB) Staining

Just out today in Science:

Electron ptychography achieves atomic-resolution limits set by lattice vibrations

I had some very old but new slides that were also covered with a layer of some white residue, stuck together and cloudy, similar to what Hobbyst46 described. Tried to clean them in a similar way, and the results were similar. What made a difference though was polishing them with toothpaste; a larger...

I will try to find how filters for oblique illumination are designed... maybe I can print them too

Here's a link to a post in this forum where a member shared a set of filter designs for 3D printing.

Anyone else fancy having a go? Yes. I have an inverted microscope that provides easy slider access to the condenser and to the back focal plane of the objective. I have the original phase sliders and also a condenser silt slider (and polarizer). The slit slider is used in conjunction with a wolasto...

Thanks Bruce. The motion of the euglenids is quite distinctive, that pellicle really imparts flexibility to this organisms; they are truly contortionists. I noticed that the movements of the organism in the OP's video were different, somehow less fluid, and clearly showed pseudopodia. Yet, its behav...