MicrobeHunter.com Microscopy Forum

Hello,

Are the DIC videos on youtube edited to add color?

I assume they have to be because DIC is in black and white usually right?

If so, whats a good software for this?

Hello, I recently starting working in a research lab as an undergrad and have worked with some pretty impressive dissecting scopes such as the nikon SNZ 1500. I perused ebay and found that they are like 5k on a good deal. My budget is more in the <$500. I want something that can give me anywhere fro...

Hey, so im looking to get oblique illumination to work as well as it does at low na <.6 at high na >.6. Whenever i try using simple oblique techniques like setting the condenser off or using a patch the effect ruins resolution on my objectives with an NA higher than .6. i am wondering if there is a ...

Like others have said im almost positive its due to the bulb. Try to get a white light LED

75RR wrote: ↑

Fri Feb 19, 2021 2:35 pm

Nice catch and video!

Still not sure why they are also called Water Bears ... Water Piglets would seem a better fit.

they are often called moss piglets as well as water bears

hello, i didnt get to see the image but i assume it was simply the blood cells drying out. I often observe this while looking at blood samples, the same phenomenon happens when the cells are in a hypertonic solution. If you are diabetic, cells often look like this when your blood sugar is high. It s...

hello, ive been looking into plasdic, as i think i will soon be ready to purchase a prism or two. So, from my understanding, rather than using the analyzer prism you replace it with a 'slit' patch stop and the length/width of the slit effects the amount of contrast? additionally, i understand that y...

okay thank you, ill return it then

hello, i realized the objective i bought is Ph2, does this mean it will have lower image quality in brightfield, darkfield and DIC?

Hello, will DIC work if the objectives are not made for DIC? If they are just neofluar or planapo but not DIC specified could those still be used if I had the slider in the tube and the other prism beneath the condenser?

it is a chinese brand scope named 'amscope'. it is this exact one: https://www.amscope.com/40x-1000x-plan-infinity-kohler-laboratory-research-grade-trinocular-compound-microscope.html I have it fitted with infinity corrected nikon objectives but i would like to upgrade those to planf or planapo exce...

i called the company and asked their parts department to measure for me

hey, i have a scope with a 180mm tube length.
Would there be anyway to use objectives for 160 or 210 on my scope?
if so, how?
Thanks.

hi, ive been looking for a good under $200 40 or 50x planapo or plan fluor objective. the only stipulation is it needs to be infinity corrected. does anyone know a good source of these? i search american and german ebay but i havent been able to find any for under 200 that were infinity corrected. i...

i was looking through todays footage and also found this:

https://www.youtube.com/watch?v=syraR853u_s

this time i kept the camera still, id recommend watching in at least 720p due to the high compression and low video quality.

i dont believe they were responding to the light beam, this phenomenon happened multiple times where i kept the slide still, i just got excited when i saw it while i was recording so i followed it to keep in frame. it happened 3 or 4 times in an hour or so with different groups of same species param...

https://www.youtube.com/watch?v=XPlWSW9PYZM

does anyone know what behavior these ciliates demonstrate?

alright, thank you, do you think my 20x .75NA planapo would do well with the aforementioned condenser? the reason i ask is because when i use DF patch stops for my 20x and 40x the resolution goes down significantly.

hi, im looking into purchasing a high NA darkfield condenser to work with NA>.65 objectives. If i bought a 1.3-1.4 oil condenser would this work with objectives with .65-.75NA (Nikon plan 40x .65 and Nikon planapo 20x .75) what would be the lower limit of functionality for this condenser?

wow, those 40x images look amazing, are they DIC?

nevermind, i contacted the seller and it turns out this was a metallurgic objective lol my bad.

also, the objective is:
https://www.ebay.com/itm/Nikon-CF-Plan- ... SwUu5b7Eqw

okay, thank you!

im using an amscope t610 with nikon objectives. Also, ive read that you dont want to hit the NA cap on the condenser and you should be below it, does this mean would be able to get an DF condenser with a NA of .7-.9 and it would work? Pretty much, how far past the NA of my objectives should the cond...

hey, im looking at some high mag (40-100x) nikon objectives and i noticed that some are very low priced and they all have a coverglass thickness of 0, would the resolution of these objectives be hindered if i used .13-.17mm coverglass? typically, they are the Nikon CF plan infinity corrected. Thanks

hey, does anyone know of a good DF condenser that has a NA of .65-.75 within its working range?

wow, thank you for the wealth of information, do you know if DIC components functionality depends on their brands matching the scopes brand? if i had an infinity corrected DIC system using olympus components could i get the same results if i moved the components to a nikon scope?

alright, thanks for the information guys, and i have never heard of reflected light DIC, ill research that, i just have a chinese unbranded scope with filter holders and koheler illumination fitted with nikon planapos and apos for 20x, 40x and 100x. The 20x is specified to work with DIC n2 prisms i ...

hi, ive been looking into DIC and buying prisms on ebay, how do i tell if a slider will work with a prism or if either will work with my objectives? and is planapo a requirement? Are brands able to cross? like if i got an olympus prism with a zeiss slider? Pretty much, what do i need to look for whe...

hey, i need an adapter for my microscope objective. i have a nikon 25M obj that i would like to fit to a nosepiece with RMS threading. Because this whole quarantine thing i am unable to ship one to the US from other countries (everyone on ebay is either canadian, russian or chinese) does anyone know...