MicrobeHunter.com Microscopy Forum

Thank you for the warm welcome everyone. I have taken everyone's comments into consideration and it is much appreciated. I recently had to learn how to set up calcium imaging in the institute and I'm having trouble figuring out the minutia of the system. Perhaps to elaborate more on my question. I ...

Thank you for the warm welcome everyone. I have taken everyone's comments into consideration and it is much appreciated. I recently had to learn how to set up calcium imaging in the institute and I'm having trouble figuring out the minutia of the system. Perhaps to elaborate more on my question. I d...

Hi Andrew, apart from the cover slip thickness the further medium between cover slip and object is part of the calculation.If there is a tiny water layer cover slips of less than 0,17mm might actually offer a better image. Apart from that: a #1 is thinner than a #1,5! So if you can't focus the prob...

To be honest, Andrew ... I had never heard of a #1 chambered coverslip :oops: So, I looked here for the spec. https://www.thermofisher.com/order/catalog/product/155361#/155361 ... and also saw the price I assume, and sincerely hope, that your colleagues know exactly what they are doing. MichaelG. ....

Hi, My name is Andrew, I'm new to the forum. I have a question about why a lab in my institution is using a #1.0 chambered coverglass for an objective lens that's meant for 0.17 thickness. I have tried using #1.5 chambered coverslip but the image never focuses. The lab does calcium signaling with F...

At the risk of being obvious, have you asked them? While someone here might have a good explanation as to why you might want to,- I don't - it's only speculation as to why they are doing it. Yes, I have asked them. The lab has been using this specific product for years. Unfortunately, I was never t...

Here is a conjecture: it is non-uniform nomenclature. 0.17mm-thick coverslips are considered to be ideal for high-NA imaging. This includes fluorescence studies (like the Fura-2). There are several mafg. of "chambered coverslips" (the term is new to me - knew them by other names). For example, Ibid...

Hi, My name is Andrew, I'm new to the forum. I have a question about why a lab in my institution is using a #1.0 chambered coverglass for an objective lens that's meant for 0.17 thickness coverslip. I have tried using #1.5 chambered coverslip but the image never focuses. The lab does calcium signali...