Search found 1202 matches

by Charles
Mon Jun 01, 2020 8:51 pm
Forum: Pictures and Videos
Topic: First attempts at arranging diatoms
Replies: 8
Views: 196

Re: First attempts at arranging diatoms

Good job Wes! Nicely done.
by Charles
Mon Jun 01, 2020 8:40 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

You would need to remove the salt first through several water changes.
by Charles
Mon Jun 01, 2020 8:37 pm
Forum: Specimens, samples and slides
Topic: Build Your Own Needel Puller, Part Two
Replies: 16
Views: 371

Re: Build Your Own Needel Puller, Part Two

I really appreciate this fine work. I assume the needle is fixed into a micromanipulator? Pictures of those would be nice to see, especially if they are self made. I use a micromanipulator made by Prior. I use a hypodermic needle to put the glass needle on and then onto a brass rod which is held by...
by Charles
Mon Jun 01, 2020 2:15 pm
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 14
Views: 269

Re: Fresh water diatoms

Beautiful Charles! I agree with 75RR. There is a lot of skill getting the shots. The processing just shows the specimen better.
by Charles
Mon Jun 01, 2020 2:13 pm
Forum: Specimens, samples and slides
Topic: Build Your Own Needel Puller, Part Two
Replies: 16
Views: 371

Re: Build Your Own Needel Puller, Part Two

OK, I'm a beginner so I need some education on this. What is the purpose of the needle? Where is the needle to be pulled? Embedded in something? Why pull the needle? to reuse it? Looking forward to learning, Hi Mary, Pulling needles means making glass needles from capillary tubes. These particular ...
by Charles
Sun May 31, 2020 2:52 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

I started cooking the sample now in Sulfuric acid. The flasks had about 30ml of sample and water and I added about 30ml of acid. I placed the flasks in a metal pot with enough water to keep it from boiling off. You don't want too much water where the flasks float off the bottom and could tip over. I...
by Charles
Sun May 31, 2020 2:44 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

I have completed about five water changes to the sample heated in HCl. I have been only waiting about four hours to let things settle and then siphon off the liquid and putting it in pickle jars. Due to short period of time letting things settle (ideally you would want to let it settle for 24 hrs) I...
by Charles
Sun May 31, 2020 12:19 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

The diatoms shop in Holland sells pre-cleaned diatom samples (Website in English) and Mountants and other gear you may need. So no unknown dangerous acids to mess around with: https://www.diatoms.nl/index.php?seo_path=samples Happy customer only, You are right deBult. I have bought stuff from him a...
by Charles
Sun May 31, 2020 12:11 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Charles, can you add a word on where you obtain your chemicals? Muriatic acid I know to be available in home improvement/hardware stores, but any comment regarding concentration? I seem to remember there being some discussion of having to be careful to buy the 'strong stuff'. And where do you get s...
by Charles
Sun May 31, 2020 11:59 am
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

when I collect diatoms silt and sand are usually my problem contents. I try to remove them by selecting after the speed in which they settle in a suspension. How do you remove stuff like silt and sand and when do you do it? Hi Bob, I do this after the material has been cleaned. Once it is cleaned a...
by Charles
Sat May 30, 2020 11:27 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Is heating necessary, or simply a way to speed the process? I frequently use HCl to clear calcium carbonate from marine equipment, but even at room temperature the acid is aggressive. I’m also curious what the sulfuric dissolves that HCl doesn’t. It’s been a while since my last chem class. :D Some ...
by Charles
Sat May 30, 2020 3:37 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Hobbyst46 wrote:
Sat May 30, 2020 1:24 pm
Watching from a safe distance... :)
Yes, I think you are at a safe distance!

Trying to HCl cook, hoping the rains will hold off.
Diatom Cooking HCl.jpg
Diatom Cooking HCl.jpg (300.76 KiB) Viewed 160 times
by Charles
Sat May 30, 2020 12:25 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Thanks. I will look into the Falcon tubes. I was going to start the acid processing and need to do it outside, since I don't have a fume hood, but the weather will not cooperate. It's suppose to rain most of the day. I want to start with the HCl 30%, to get rid of any calcium carbonates and any meta...
by Charles
Sat May 30, 2020 11:53 am
Forum: For forum members who want to buy and sell equipment
Topic: Newbie with Antique Carl Zeiss HELP!!!
Replies: 4
Views: 151

Re: Newbie with Antique Carl Zeiss HELP!!!

It was probably made around the turn of the century since it has a black enameled base. The back of the base is oddly shaped. Would need a serial number to date it correctly. Look closely at the tube under the Carl Zeiss name to see if you can see a serial number. Can you put it together and post pi...
by Charles
Fri May 29, 2020 2:19 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Hi Charles, I wouldn't have expected that to happen with a PE or PP bottle. Perhaps you could use a silicone form for ice cubes or choclate making? Bob Bob, Yes, I think using open topped containers like ice cube trays or just storage dishes would work better. It not only has lots of room to expand...
by Charles
Thu May 28, 2020 10:21 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

Hobbyst46 wrote:
Thu May 28, 2020 10:08 pm
In general (Wikipedia) PP tends to be brittle upon freezing...
Good thing I have lots of spares. Surprisingly the 5ml tubes have not cracked even after about 30 cycles.
by Charles
Thu May 28, 2020 10:15 pm
Forum: My microscope
Topic: New Microscope. Help identify?
Replies: 10
Views: 200

Re: New Microscope. Help identify?

I sent you the manuals to both emails.
by Charles
Thu May 28, 2020 9:52 pm
Forum: My microscope
Topic: New Microscope. Help identify?
Replies: 10
Views: 200

Re: New Microscope. Help identify?

I will also send you an instruction sheet for the DIC system and your 12v 60W lighting system.
by Charles
Thu May 28, 2020 9:40 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

I don't know what brand they are but they were inexpensive. I noticed that even though I left an air gap for expansion, a couple of the samples actually expanded it's volume in the tube during the freeze/thaw cycle.
by Charles
Thu May 28, 2020 9:34 pm
Forum: My microscope
Topic: New Microscope. Help identify?
Replies: 10
Views: 200

Re: New Microscope. Help identify?

Nico, If you post your email, I have a manual for the GFL I can send you.
by Charles
Thu May 28, 2020 9:24 pm
Forum: My microscope
Topic: New Microscope. Help identify?
Replies: 10
Views: 200

Re: New Microscope. Help identify?

You will need a 12V Zeiss power regulator like this one: https://www.ebay.com/itm/CARL-ZEISS-MOD ... Sw6f1esIVR
The phototube will allow you to use your DSLR but you may need to find a proper mount for your model of camrea.
by Charles
Thu May 28, 2020 9:15 pm
Forum: My microscope
Topic: New Microscope. Help identify?
Replies: 10
Views: 200

Re: New Microscope. Help identify?

Welcome Nico,

You have a very nice Zeiss GFL with DIC. There is the old style DIC single beam slide in the top intermediate piece and the old style DIC condenser with the white arrow. I don't know what the black cable is but the 12V 60 lamp will need a power regulator.
by Charles
Thu May 28, 2020 4:44 pm
Forum: Specimens, samples and slides
Topic: New Diatom Project
Replies: 37
Views: 605

Re: New Diatom Project

I've gone through about 25 freeze/ thaw cycles with the 5ml tubes and decided to process more material, while I await the arrival of my 250ml flasks to do the rest of the acid processing. So I put some raw material into a 10ml plastic tube for the freeze/thaw cycles. Got about 10 cycles and one of t...
by Charles
Wed May 27, 2020 9:48 pm
Forum: Collecting microscopes and slides
Topic: Just purchased a bunch of mid-century Zeiss components, need help identifying
Replies: 19
Views: 221

Re: Just purchased a bunch of mid-century Zeiss components, need help identifying

And here are the two different DIC systems, one with the single beam slide and the other with individual objective DIC slides:
Zeiss DIC sets.jpg
Zeiss DIC sets.jpg (72.13 KiB) Viewed 106 times
by Charles
Wed May 27, 2020 6:41 pm
Forum: Collecting microscopes and slides
Topic: Just purchased a bunch of mid-century Zeiss components, need help identifying
Replies: 19
Views: 221

Re: Just purchased a bunch of mid-century Zeiss components, need help identifying

The Optovar one would need a analyzer :lol: I somehow forgot about the existence of the new DIC system, you are correct. So in this case how do you get an analyzer inside the piece with the Optovar? Is it a makeshift solution or is there an intrinsic design feature that allows it? Yes, some (but no...
by Charles
Wed May 27, 2020 6:19 pm
Forum: Collecting microscopes and slides
Topic: Just purchased a bunch of mid-century Zeiss components, need help identifying
Replies: 19
Views: 221

Re: Just purchased a bunch of mid-century Zeiss components, need help identifying

In the first photo, the two large round items are intermediate pieces, which goes between the viewing head and objectives. The left one is for analyzers but also can be used for a single DIC beam slide (the insert in it now is an analyzer which would also need a condenser with polarizer to give pol...
by Charles
Wed May 27, 2020 2:34 pm
Forum: Specimens, samples and slides
Topic: Build Your Own Needel Puller, Part Two
Replies: 16
Views: 371

Re: Build Your Own Needel Puller, Part Two

Those are some fine glass needles Wes! This looks all very fine. However, as I know nothing about diatomes, could you explain for what purpose you actually use these? Is "needle" solid or are these capillaries? If capillaries, you could make microinjections to egg cells :) . I am just curious... The...
by Charles
Wed May 27, 2020 2:26 pm
Forum: Collecting microscopes and slides
Topic: Just purchased a bunch of mid-century Zeiss components, need help identifying
Replies: 19
Views: 221

Re: Just purchased a bunch of mid-century Zeiss components, need help identifying

In the first photo, the two large round items are intermediate pieces, which goes between the viewing head and objectives. The left one is for analyzers but also can be used for a single DIC beam slide (the insert in it now is an analyzer which would also need a condenser with polarizer to give pola...
by Charles
Wed May 27, 2020 2:06 pm
Forum: Beginner's corner
Topic: Microscope Dust Cover
Replies: 5
Views: 93

Re: Microscope Dust Cover

I've used plastic bags to nylon bags. They say plastic bags could be bad, especially in humid climates because it holds moisture i. nSomething like this is probably optimal. Although it says it's plastic, it's probably rip-stop nylon used in parachutes. I got a few and like them. https://www.ebay.co...
by Charles
Mon May 25, 2020 9:45 pm
Forum: My microscope
Topic: Brinkmann
Replies: 9
Views: 2743

Re: Brinkmann

Nice diatom shot.

Brinkmann rebranded/put their name, on a lot of microscopes they didn't make. The microscope has Made in Germany under the focus knob and was probably made by Beck Kassel.