Search found 502 matches

by hans
Wed Nov 25, 2020 4:33 am
Forum: Beginner's corner
Topic: Tripod exanding and contracting with 3°C temp swings when shooting timelapses at 5x?
Replies: 3
Views: 53

Re: Tripod exanding and contracting with 3°C temp swings when shooting timelapses at 5x?

With a distinctive target like a ruler with fine graduations, and with the camera in live view mode zoomed in all the way, maybe you can see the motion in real time relative to fixed guide lines or focus area boxes or whatever on the screen? If so you could try to narrow down where the sensitivity i...
by hans
Wed Nov 25, 2020 3:16 am
Forum: Identification help
Topic: Colpoda?
Replies: 6
Views: 120

Re: Colpoda?

Thanks Bruce. Do you know if ciliates like these actively change cell shape to help fit around obstacles? I know intuition about how stuff moves doesn't scale down well, but after watching them navigate the tight spaces for a while I get the sense they are deforming a lot more than would be expected...
by hans
Tue Nov 24, 2020 9:13 am
Forum: Identification help
Topic: Naked bicosoecid?
Replies: 0
Views: 30

Naked bicosoecid?

Video is normal speed with gamma adjustment to enhance contrast. 100x objective, 3.5 um diameter, trailing flagellum is 7 um long:
Image
by hans
Tue Nov 24, 2020 2:48 am
Forum: Identification help
Topic: Heterolobosean amoeboflagellates?
Replies: 2
Views: 66

Re: Heterolobosean amoeboflagellates?

After watching a few more I really think the swirling/tumbling motion is internal. There is no apparent disturbance of stuff surrounding the cell and there appears to be quite a bit of relative among contents at different depths within the shell, inconsistent with the entire cell rotating and the co...
by hans
Tue Nov 24, 2020 2:22 am
Forum: Pictures and Videos
Topic: Pond Life!
Replies: 12
Views: 203

Re: Pond Life!

LouiseScot wrote:
Sun Nov 22, 2020 9:21 pm
(Anyone know what the filaments with the moving granules are?)
Possibly fungal?


by hans
Mon Nov 23, 2020 9:34 pm
Forum: Identification help
Topic: Colpoda?
Replies: 6
Views: 120

Re: Colpoda?

Made a slide with some small flakes of growth scraped from the side of the sample jar. The unbroken flakes are dense enough to create a large number of small voids, restraining their movement a bit, so I could get some video at 100x: https://storage.googleapis.com/microscope-share/DSC_9422.jpg In th...
by hans
Sat Nov 21, 2020 9:05 am
Forum: Identification help
Topic: Colpoda?
Replies: 6
Views: 120

Re: Colpoda?

Sorry, forgot to specify size: 40x objective, camera is covering most of the FOV diagonally, and the ciliates are around 30 by 15 by 10 um. Hadn't seen Chilodonellidae but some photos do look similar. The Colpoda guess I was basing a lot on the style of movement which looks very similar to some vide...
by hans
Sat Nov 21, 2020 9:04 am
Forum: Identification help
Topic: Heterolobosean amoeboflagellates?
Replies: 2
Views: 66

Heterolobosean amoeboflagellates?

Previous, possibly-related observations: Green algae (?) with steady internal swirling motion (possibly cysts) An amoebozoan in order Leptomyxida? (no, probably class Heterolobosea) Euglenid? (possibly the flagellate form) Amoeba video processing (more examples of sustained, eruptive movement charac...
by hans
Sat Nov 21, 2020 3:25 am
Forum: Camera systems and imaging
Topic: Need help connecting a Nikon Coolpix L120 to a microscope
Replies: 32
Views: 919

Re: Need help connecting a Nikon Coolpix L120 to a microscope

Always nice when the fix turns out to be simple. Mostly mentioning this out of my own curiosity, in case you feel like experimenting further, but this part still seems suspicious: Now that meant that the C-mount would go TOO far down, but that is ok, because part A can be unscrewed/raised by a large...
by hans
Fri Nov 20, 2020 9:52 pm
Forum: Camera systems and imaging
Topic: Need help connecting a Nikon Coolpix L120 to a microscope
Replies: 32
Views: 919

Re: Need help connecting a Nikon Coolpix L120 to a microscope

Sounds like that pretty well rules out a binocular vs. photo tube mismatch. Another thought, to measure the required displacement more accurately, could you focus the camera in the photo port, then put and eyepiece in the photo port and see how far it is necessary to lift the eyepiece to get focus? ...
by hans
Fri Nov 20, 2020 8:20 pm
Forum: Camera systems and imaging
Topic: Need help connecting a Nikon Coolpix L120 to a microscope
Replies: 32
Views: 919

Re: Need help connecting a Nikon Coolpix L120 to a microscope

Hmm, yeah what I said about unscrewing the lens was backwards, but it sounds like you already understood that. I don't have any other suggestions, but permanently shortening the tube may not be a good idea until the camera/lens are conclusively ruled out, otherwise you will have a problem in the fut...
by hans
Fri Nov 20, 2020 8:02 pm
Forum: Identification help
Topic: Colpoda?
Replies: 6
Views: 120

Colpoda?

Apparently very common but just started showing up in my cultures from dew-watered spots in the last couple days. Finally enough around to get decent video, were too difficult to track down and follow around when they were more scarce:
Image
by hans
Fri Nov 20, 2020 6:14 pm
Forum: Camera systems and imaging
Topic: Need help connecting a Nikon Coolpix L120 to a microscope
Replies: 32
Views: 919

Re: Need help connecting a Nikon Coolpix L120 to a microscope

A few other thoughts: Did you buy the camera and lens preassembled together as a unit? Is it possible a C/CS mount spacer ring between the camera and lens is either missing, or present but not supposed to be there? On some of my older C-mount cameras there is a non-obvious way to focus -- loosen set...
by hans
Fri Nov 20, 2020 5:12 pm
Forum: Camera systems and imaging
Topic: Need help connecting a Nikon Coolpix L120 to a microscope
Replies: 32
Views: 919

Re: Need help connecting a Nikon Coolpix L120 to a microscope

In addition to tube length of the photo port there are at least two other things that could be off to cause this: position of the intermediate image relative to the top of the tube the camera/lens is inserted into (property of the camera/lens) and tube length going to the eyepieces. Some questions t...
by hans
Fri Nov 20, 2020 2:29 am
Forum: Pictures and Videos
Topic: Amoeba bites more than it could chew.
Replies: 6
Views: 98

Re: Amoeba bites more than it could chew.

Nice action shot. Some of the dark-outline granules in the amoeba looks suspiciously similar to ones in the sessilid -- possibly acquired from previous victims?
by hans
Wed Nov 18, 2020 12:11 am
Forum: Pictures and Videos
Topic: Amoeba video processing
Replies: 9
Views: 237

Re: Amoeba video processing

My own suspicion is that they are all the same heterolobosean species... Browsing around some more, I noticed this: https://www.arcella.nl/naegleria-gruberi/ The size of cysts matches (10 um) and they look similar (in particular, "perinuclear layer of coarse granules") as the things (from the same ...
by hans
Tue Nov 17, 2020 8:31 pm
Forum: Pictures and Videos
Topic: Rotifer (Dissotrocha macrostyla)
Replies: 7
Views: 183

Re: Rotifer (Dissotrocha macrostyla)

Nice, clear video of the feeding action. Looks like cilia leading down to the esophagus are visible a few times? Reminiscent of a little wood chipper. The ones in my sample seem to only feed while crawling/swimming and thrusting their heads around making it difficult to get good video of the trochi ...
by hans
Tue Nov 17, 2020 8:25 pm
Forum: Pictures and Videos
Topic: Amoeba video processing
Replies: 9
Views: 237

Re: Amoeba video processing

Great video, Hans! I showed it to a friend (an expert in amoeboids), who confirms that this is a heterolobosean, showing classic eruptive pseudopod formation. So, it is an amoeba, in the casual sense, but not in the group Amoebozoa. Thank you, Bruce. I have been trying to be careful about the amoeb...
by hans
Mon Nov 16, 2020 10:17 pm
Forum: Pictures and Videos
Topic: Trophi in bdelloid rotifer carcass
Replies: 32
Views: 1120

Re: Trophi in bdelloid rotifer carcass

Wow that ciliate it eats is almost as big is its head. I see the ones in my sample feeding a lot but not visibly ingest anything. I think they are eating much smaller stuff. Found another contracted one nicely presenting its resolution test targets close to the cover glass. Spacing of the finer ridg...
by hans
Mon Nov 16, 2020 7:33 pm
Forum: Home-made microscope adaptations
Topic: LED Lighting and optical resolution questions
Replies: 86
Views: 2320

Re: LED Lighting and optical resolution questions

It looks like the ToupTek software may be boosting contrast quite a bit more than Canon? Also looks like the Canon image may be suffering more from noise and subsequent noise reduction? What ISO setting are you using on the Canon? I do not get the impression that pixel size is currently the limiting...
by hans
Mon Nov 16, 2020 7:28 pm
Forum: Pictures and Videos
Topic: Amoeba video processing
Replies: 9
Views: 237

Re: Amoeba video processing

I will have to give Shotcut a try. Mainly using Avidemux because I have used it in the past for really simple stuff so already somewhat familiar with the UI. I did try Kdenlive but found the UI with large number of tiny buttons a bit cumbersome for simple usage. Main frustration with Avidemux so far...
by hans
Mon Nov 16, 2020 6:27 am
Forum: Pictures and Videos
Topic: Amoeba video processing
Replies: 9
Views: 237

Amoeba video processing

Some more messing around with speed-up and contrast enhancement of amoeba video: https://storage.googleapis.com/microbehunter-share/DSC_9308.jpg 100x objective, video recorded with Nikon D5100 which has some annoying quirks related to video mode but seems to produce good quality video once set up. H...
by hans
Mon Nov 16, 2020 4:06 am
Forum: Pictures and Videos
Topic: Trophi in bdelloid rotifer carcass
Replies: 32
Views: 1120

Re: Trophi in bdelloid rotifer carcass

My very uninformed guess is that may be a rotifer but not a bdelloid, which is the only type I have found and read about so far. I believe it is only bdelloids that have this type of ridged trophi and while most other rotifers have trophi they are generally not so distinctive or easily seen? I don't...
by hans
Mon Nov 16, 2020 3:09 am
Forum: Pictures and Videos
Topic: Another Bursting
Replies: 8
Views: 260

Re: Another Bursting

Normally labeled "#1", "no. 1" or with thickness range "0.13 - 0.16 mm" but I am not sure what a good source would be in Canada. Where do you usually buy microscope stuff?
by hans
Mon Nov 16, 2020 12:07 am
Forum: Pictures and Videos
Topic: Another Bursting
Replies: 8
Views: 260

Re: Another Bursting

DonSchaeffer wrote:
Sat Nov 14, 2020 10:10 pm
It may be the 60 X objective that does it.
Sort of looks like it from the video. Have you considered trying thinner #1 cover glass rather than the more common #1.5? Gives a little more room to focus without squishing stuff.
by hans
Sun Nov 15, 2020 9:43 pm
Forum: Microscopes and optics
Topic: Broken Microscope
Replies: 20
Views: 4495

Re: Broken Microscope

...horizontal gizmo sticking out from the head opposite the angled mono viewing tube. Searching the web for a manual, I found the one shown in the Broken Microscope posts but nothing with a head shown in the attached photo. What does it do and could it complicate the repair? Illuminated pointer for...
by hans
Sun Nov 15, 2020 9:35 pm
Forum: Home-made microscope adaptations
Topic: Homemade phase annuli
Replies: 13
Views: 2537

Re: Homemade phase annuli

Nice work, I am following with interest, having recently bough a pair (10x and 40x) of "for parts or not working" phase objectives for my Microstar IV to mess with.
by hans
Sun Nov 15, 2020 9:28 pm
Forum: Home-made microscope adaptations
Topic: LED Lighting and optical resolution questions
Replies: 86
Views: 2320

Re: LED Lighting and optical resolution questions

I'm afraid I'm not a very good judge of these things. I thing it's quite a difficult subject since the contrast is low. I've adjusted contrast and brightness somewhat. Also, Nikon 20x/0.75 is difficult to focus because it has a miniscule depth of focus. ... Someone mentioned previously I had a lot ...
by hans
Sun Nov 15, 2020 5:38 pm
Forum: Pictures and Videos
Topic: Brown Hydra & unfortunate Ciliate
Replies: 7
Views: 251

Re: Brown Hydra & unfortunate Ciliate

Nice video. Sort of looks like there are bulges of clear fluid swelling around the ciliate in the bright field shot 2:45 to 3:45. Any idea what that is? Related to the damage from the hydra?
by hans
Sun Nov 15, 2020 5:29 pm
Forum: Pictures and Videos
Topic: Trophi in bdelloid rotifer carcass
Replies: 32
Views: 1120

Re: Trophi in bdelloid rotifer carcass

Thanks Michael. I'm not sure, whether it was somehow accelerated by your procedure, but the young girl seems to feel quite good. Yeah, I suspect it was not a coincidence since it happened so quickly. Possibly pressure from the cover glass but the casing was intact when I first rehydrated it, not muc...