MicrobeHunter.com Microscopy Forum

Hi Hobbyst46, Thank you very much for your comment. I think the distortion may came from S10E camera, I use S10E take photo via Afocal technic. The S10E camera lens has equivalent to 26 mm focus length, it is consider wide angle lens. So it may cause distortion when look into the eyepiece. I may try...

This is another Diatom sample by 60X Objective at 600X magnification, Oblique illumination. Single shoot (no stacking), Noise reduction to reduce background noise. The Diatom was leave it to slowly dry under cover slip for a few weeks. I calculate it size from micrometer scale around 35 Micrometer l...

I have a chance to purchase Micrometer slide at last. So I can take a photo of micrometer scale and calculate size of sample under the same objective magnification easily. Here is photo of micrometer scale at 60X Objective with 10X Eyepiece. 600X total magnification. Photograph by using smart Phone ...

Hi Gerard,


Thank you very much for your comment and information. This prepared slide did not contain any more details than the label that show Testis of Fish.

Your details information will be very useful for all members.



Cheers,


Suphot

Hi John B,


Thank you very much for your comment, it is my first time to see chromosome of the fish.



Suphot

I purchased 200 prepared slides from ebay (similar to AMscope prepared slides set) and look at it for my free time.

This Testis of Fish TS slide, it look like cluster of chromosome and some of them was in separation phase.

600X, Afocal photography using Samsung Galaxy S6


Suphot

Very sharp and fine details photo, it look 3 dimensions more than photo.



Suphot

The 3rd photo was very colorful.



Suphot

Very beautiful and clear.


Suphot

Thank you very much Mike, I don't know about Variable Asymmetrical Contrast before. It look very good in your photo. I have to find the polarize sheet to try this one. You may post your photo from this technique some more. The Oblique illumination that I used, came from GUF Filter or Offset my Dark ...

Very Beautiful,

The first one look like star in the sky.


Suphot

Thank you very much for all comments and sharing your opinion.

The photo from photomicro was very sharp and clear. You may share more information about your set up and illumination technique.


Suphot

Thank you very much for your comment 75RR and billbillt,


75RR, I have not a chance to measure it, the Oblique illumination look 3 dimensions to me especially the small water drop near diatom.


billbillt, Yes, I like the effect and it look like DIC in some degree, for 40X Objective.



Suphot

Diatom Sample at 640X (40X Objective x 16X Eyepieces) Single shot, Crop and Adjust Contrast / Brightness Diatom was wet mount, but the surrounding water was dry up, some small drop can be seen in the photo. The 40X Objective work well for Oblique Illumination. The 16X Eyepieces magnify a little more...

Hi Bob, Thank you very much for your information, I may try chinese micrometer sometime. My test sample was a simple wet mount and try to see how the objective can show the details. I may not perform the critical test as you mention, according to my limit experience. By the way, it is good to know s...

Hi coominya, You may need some support to your smart phone to easier focus. I use smart phone zoom to enlarge the picture in screen and focus it. Then zoom back to 1X and take photo. It is a hit and miss (some photo sharp, some photo not so sharp). My LED is white and look a liitle blue, so I think ...

Hi Vasselle,

Thank you very much for your comment.

Your beautiful photo always the one that I enjoy to see.


Suphot.

Hi 75RR,

Thank you very much for your recommend, I will check on it.

Your sample photo is very sharp, very high resolution. It is a good reference one.


Suphot.

Hi Bob, The Diatom still wet, but the water around it was dry up, you can see some small drop remaining on top of the photo. After a few minute, I saw water inside th Diatom dry up. But I am not take the photo when it was dry. This Diatom sample was quite flat, so I can focus it sharp almost the who...

Hi Bob, Thank you very much for your comment. My set up for DF is not work well for 40X Objective at the first time. I found that the Condenser is not properly align with the Objective. So I have to loose the Condenser mounting screw a little bit and centering it to the Objective. Then it work well ...

Hi coominya,

Thank you very much for your comment. My microscope use LED and I use camera setting to auto in my smart phone. I focus by seeing on the smart phone screen. The smart phone is Samsung Galaxy S6.

Suphot

Now I found a clear Diatom to test with this Dry 60X Objective. I use 16X Eyepieces so the total magnification is 960X.

Afocal Techic by using smart phone take photo from Eyepieces.

60X Objective X 16X Eyepieces (960X)
Bright Field Illumination

Resize and Unsharp Mask

When I swing the DF patch in the filter tray off center a little bit, it give me Oblique Illumination picture, which is also looking good.

I have 16X Eyepieces that came with the microscope, it is not in use much. When I try Dark field technic with simple patch made from transparency with black dot glue in the center. I can use Dark Field patch up to 40X Objective. With 10X Eyepieces it give me 400X magnification. But I cannot use DF p...

Hi MicroBob,

Thank you very much for your comment and for post the original photo of Human Blood smear.

It is very good to see it in Negative filter. Sometime it look like plant cell when the color is green.


Cheers,


Suphot

Hi MicroBob,

I did not prepare the slide by myself. I bought this prepared slides set from ebay. Your slide is much clearer and contain more details, very beautiful one.



Suphot

Very beautiful color and sharp image. You can go on for more


Suphot

Human Blood smear, Prepared slide at 400X (40X Objective x 10X Eyepiece)
Afocal Photography using smart phone, zoom in about 2X at smart phone. (Look like 800X Magnification)

Oblique Illumination and Digital Process by using Negative filter effect.

Thank you very much for your comment, charlie g and Radazz, I capture photo by using simple DIY smart phone adapter with small tripod to hold it in place over microscope eyepiece. You can see the details of DIY smart phone adapter in Microbehunter Magazine Volume 2 Number 11, November 2012. This DIY...

Thank you very much for your comment, MichaelG. , Jonnyvine, and John B. I have ever try Onion root tips and squash it for wet mount. But I can not find clearly chromosome in the root tip sample. Only saw nucleus of Onion cell. But this prepared slide of Lily Ovary has some chromosome in it. Suphot