MicrobeHunter.com Microscopy Forum

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Very beautiful video Sansub2, the 2nd video which has blue background is very nice.


cheers,


Suphot.

Thank you very much for your comment and video, Sansub2.

Your video is beautiful sample, I can see detail inside Paramecium clearly.
I'm glade to hear that this Oblique filter work well with your microscope.

Hopefully it work well with your WI 40X.


Cheers,

Suphot.

Thank You very much for your comment Ross, I hope it work well for your Objective. Please share your result if it is available. Thank you very much for your comment MichaelG. My microscope has simple Abbe condenser, it clearly show Chromatic aberration that you saw in the picture. I just don't know ...

Note: This small water droplet is very difficult to see under Bright Field Illumination. It is colorless and small under cover slip in my wet mount sample. It is easier to see when using the Oblique Illumination Filter and make the droplet look 3D Image. All photo samples above made by Afocal Photog...

Pic 8. Show 60X Objective with Oblique Illumination Filter (Total Magnification is 600X with 10X Eyepieces).

Pic 7. Show 60X Objective Bright Field image (Total Magnification is 600X with 10X Eyepieces).

Pic 6. Show 40X Objective with Oblique Illumination Filter (Total Magnification is 400X with 10X Eyepieces).

Pic 5. Show 40X Objective Bright Field image (Total Magnification is 400X with 10X Eyepieces).

Pic 4. Show 20X Objective with Oblique Illumination Filter (Total Magnification is 200X with 10X Eyepieces).

Pic 3. Show 20X Objective Bright Field image (Total Magnification is 200X with 10X Eyepieces).

Using this Oblique Illumination Filter is the same way as GUF Filter. It is a trial and error approach, first step you have to fully open the condenser diaphragm and slide the filter in to the condenser filter tray. Then slide back and forth, Left and Right until you get the optimum result. You may ...

I found that, if I remove the Gradient area from the GUF filter, by cut the Black Poster paper in to “L” shape like this. It seem to work well for Oblique Illumination with 40X and 60X Objective. Pic 2. Oblique Filter size, cut from Black Poster paper. The edge of cut paper is white, So I reduce sca...

After I made Gradient Universal Filter (GUF) and use it for a while for Oblique Illumination, it is not work very well with High N.A. Objective (40X and Higher). So I try to improve it to use with High N.A. 40X and 60X dry Objective that I have. Pic 1. GUF Filter size that I made by stacking 4 layer...

I did not fusing diatom on slide, but let it slowly dry under cover slip. It can kept diatom several months. For details you may read my post from here; https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=10&t=12781 microbob comment that the diatom may be clean by using alcohol burner. I ...

Thank you very much for your comment Hobbyst46 Your suggestion about other dissolve solution is very interesting. I think it will be benefit to members who would like to try Rosin for mountant. For amateur, Rosin may be one of alternative for permanent mount. I think it is cheap, easy to make and wo...

I did not see Rosin crystalize under cover slip in my sample slides and I still can observe it quite well under microscope. If you are looking for cheap and simple permanent mountant , I think you may try Rosin dissolve in Xylene. It may not suitable with some kind of samples, because I did not try ...

Compound eyes of Butterfly 400X

Compound eyes of Butterfly 100X

Plant Stem 400X

It was more than 40 years now, I kept these slides in the box and forgot about it. the slides still looking OK, the Rosin mountant become more yellow as you can see in the photo.

Under microscope, cross section of plant stem and compound eye of butterfly sample still looking good.

Plant Stem 100X

I would like to share my experience about using Rosin dissolve in Xylene for permanent slide mount. Back in 1977, I experiment to made permanent slide for my Biology class at that time. I know that Canada Balsam is a good permanent mountant, but it is very expensive and difficult to buy one. I think...

Thank you very much for all comments, Charles; I afraid to seal cover slip with vanish or finger nail polish because it may leak in to diatom and made it more difficult to see. Hobbyst46, Yes, it is difficult to avoid capillary effect, so I use scotch tape to fix cover slip in place. MicroBob, Thank...

But this is not for serious observation of diatoms, because diatoms was dry under cover slip which contain thin layer of air. So it is not give good result with 100X Oil objective. I think it is a simple and easy way to keep diatoms sample for a while. It need not complicate process and high refract...

Now it was more than 6 – 7 months, I still can observe diatoms sample in this slide. It look OK for me under 60X dry objective.

So I use thin strip of scotch tape to fix 4 sides of the cover slip to slide. I don’t know how long it can be kept in this way. I can store this slide with algae and diatom vertically in the slide box in the same way as permanent slide.

But cleaning and permanent mounting them is complicate and difficult, so I let it slowly dry under cover slip in the room.

After several day, I observe it under microscope, it is still looking OK. Some way some how, when it was dry up, algae and diatoms was fix on slide under cover slip.

I saw some algae on water tray, so I sampling them on slide by wet mount. It contain some diatoms which I think it is good to keep it for a while.

Hi Hobbyst46, Thank you very much for your comment. I think the distortion may came from S10E camera, I use S10E take photo via Afocal technic. The S10E camera lens has equivalent to 26 mm focus length, it is consider wide angle lens. So it may cause distortion when look into the eyepiece. I may try...

This is another Diatom sample by 60X Objective at 600X magnification, Oblique illumination. Single shoot (no stacking), Noise reduction to reduce background noise. The Diatom was leave it to slowly dry under cover slip for a few weeks. I calculate it size from micrometer scale around 35 Micrometer l...