Search found 107 matches

by mazo4033
Fri Jan 07, 2022 11:23 pm
Forum: Specimens, samples and slides
Topic: Containers for FAA fixed plants
Replies: 7
Views: 3139

Re: Containers for FAA fixed plants

20ml glass scintillation vials
This is a good suggestion, thank you Leonard! Definitely smaller and easier to store than the wider jars, I think.
by mazo4033
Mon Jan 03, 2022 2:44 am
Forum: Specimens, samples and slides
Topic: Containers for FAA fixed plants
Replies: 7
Views: 3139

Re: Containers for FAA fixed plants

These have that insert too. Glad I can use the jars! Thank you for the advice!
by mazo4033
Mon Jan 03, 2022 2:08 am
Forum: Specimens, samples and slides
Topic: Containers for FAA fixed plants
Replies: 7
Views: 3139

Re: Containers for FAA fixed plants

as the acetic acid chewed right through the steel cap. That's why I want to make sure I use the right jar! :shock: Plastic cap and either glass or plastic container - must be airtight, which is more likely with softer plastic caps Thank you! I have these jars, the cap and the jar are plastic (I thi...
by mazo4033
Wed Dec 29, 2021 12:36 am
Forum: Specimens, samples and slides
Topic: Containers for FAA fixed plants
Replies: 7
Views: 3139

Containers for FAA fixed plants

Hi everyone,

I was wondering what the best container is for long-term storage of plants that are fixed in FAA (to be stored for later processing and sectioning). Should these containers be plastic or glass? On a side note, what type of container should be used to store the FAA itself?

Thanks!
by mazo4033
Wed Dec 29, 2021 12:32 am
Forum: Specimens, samples and slides
Topic: Advice for sampling
Replies: 6
Views: 2466

Re: Advice for sampling

Thank you crb5 and MicroBob. The plankton net is a good idea.
by mazo4033
Sat Dec 25, 2021 7:54 pm
Forum: Specimens, samples and slides
Topic: Advice for sampling
Replies: 6
Views: 2466

Re: Advice for sampling

Thank you both for the advice. Culturing/feeding the container is a good idea, and I will make sure to keep more plants in the sample!
by mazo4033
Wed Dec 22, 2021 10:45 pm
Forum: Specimens, samples and slides
Topic: Advice for sampling
Replies: 6
Views: 2466

Advice for sampling

Hi all, I've run into a sort of problem with sampling water. I take water samples and they yield few microorganisms. I aim to sample from water that shows signs of life (algae growing on the surface, plants around the edge of a pond, plants in the water, etc). I also sample from water that looks kin...
by mazo4033
Sat Aug 21, 2021 10:16 pm
Forum: Pictures and Videos
Topic: Buxus sp. TS stained
Replies: 0
Views: 3577

Buxus sp. TS stained

Buxus sp. , a favorite of mine because its common name is "boxwood," and the transverse sections are rather box-shaped! :lol: But the wood from the shrub was commonly used to make boxes, among other pieces of woodworking, which is where the common name really comes from. 1 - introduction https://lh...
by mazo4033
Sat Aug 21, 2021 1:16 am
Forum: Pictures and Videos
Topic: Platycola decumbens = "hot water bottle animal" :-)
Replies: 6
Views: 5315

Re: Platycola decumbens = "hot water bottle animal" :-)

Amazing documentation and lovely photos!! A very interesting organism.
by mazo4033
Mon Aug 16, 2021 4:01 pm
Forum: Identification help
Topic: I have no idea
Replies: 6
Views: 3227

Re: I have no idea

Perhaps Clathrulina
by mazo4033
Thu Aug 05, 2021 11:33 pm
Forum: Identification help
Topic: What is this thing?
Replies: 9
Views: 3834

Re: What is this thing?

This is a very interesting video and a phenomenal capture, Javier! I think I have come to a conclusion of what this could be: Volvocacean algae infected by chytrids. See this paper for more info and pictures that look a lot like yours: https://www.researchgate.net/publication/325110064_Diversity_and...
by mazo4033
Tue Aug 03, 2021 4:25 pm
Forum: Pictures and Videos
Topic: Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain
Replies: 6
Views: 2263

Re: Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain

Thanks, Kevin! I look forward to seeing your results when you try some sectioning and staining!
by mazo4033
Tue Aug 03, 2021 2:51 pm
Forum: Pictures and Videos
Topic: Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain
Replies: 6
Views: 2263

Re: Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain

Thank you, John!

Interesting arrangement you describe, I’d like to find that sometime!
by mazo4033
Mon Aug 02, 2021 8:11 pm
Forum: Pictures and Videos
Topic: Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain
Replies: 6
Views: 2263

Brassica rapa var. parachinensis TS safranin-o and crystal-violet stain

Hi everyone, I have been drawn to the bright yellow flowers of the yu choy plant so I have spent some time sectioning and staining their stem, using the opportunity to test some crystal-violet stain. 1 - background Yu choy/choy sum, or Brassica rapa var. parachinensis , is a vegetable very similar t...
by mazo4033
Fri Jul 30, 2021 11:38 pm
Forum: Pictures and Videos
Topic: Ligustrum spp. TS safranin-o stain
Replies: 10
Views: 3516

Re: Ligustrum spp. TS safranin-o stain

Definitely - TBO is a superb stain, especially for live (i.e. unprocessed as per hand-sectioned) tissue. Make sure you rinse it well after staining to get the very best from TBO - take the time (only a matter of say 10 minutes really) to rinse well with water (preferable deionised - whatever water ...
by mazo4033
Fri Jul 30, 2021 6:15 pm
Forum: Pictures and Videos
Topic: Ligustrum spp. TS safranin-o stain
Replies: 10
Views: 3516

Re: Ligustrum spp. TS safranin-o stain

Thank you jimur, perrywespa, and John! Perry: very nice result with the sweet gum section!! I like the tonal shifts with the blues and the section came out very nicely. John: The metachromatic aspect of safranin is my favorite part; it creates these layers of colors that are really nice (especially ...
by mazo4033
Thu Jul 29, 2021 11:08 pm
Forum: Camera systems and imaging
Topic: Microscope Digital Camera Image Quality Comparisons
Replies: 10
Views: 4627

Re: Microscope Digital Camera Image Quality Comparisons

Hi AntoniScott, I unfortunately don’t have much advice to give on how to overcome image quality challenges, although in terms of camera comparisons, fellow forum member Rob Berdan did a very good and in-depth camera comparison here: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=...
by mazo4033
Thu Jul 29, 2021 10:42 pm
Forum: Pictures and Videos
Topic: Ligustrum spp. TS safranin-o stain
Replies: 10
Views: 3516

Ligustrum spp. TS safranin-o stain

Hi all, Recently I began botanical sectioning, and I give my thanks to forum members mrsonchus and MicroBob for their excellent and very helpful posts on this subject matter. After experimenting with various plants nearby, I found that the privet bush ( Ligustrum spp. ) happens to be a particularly ...
by mazo4033
Sat Jul 17, 2021 1:38 pm
Forum: Pictures and Videos
Topic: Pleodorina
Replies: 7
Views: 2329

Re: Pleodorina

Very lovely!!
by mazo4033
Wed Jul 14, 2021 8:29 pm
Forum: Identification help
Topic: Please help with identification of a very small circular organism
Replies: 6
Views: 5110

Re: Please help with identification of a very small circular organism

I have accidentally found what I think is the identification for this organism: Phacotus lenticularis!
by mazo4033
Wed May 12, 2021 6:18 pm
Forum: Pictures and Videos
Topic: Conjugation of oxytrichidae
Replies: 2
Views: 1185

Re: Conjugation of oxytrichidae

Thanks Jimbo!
by mazo4033
Wed May 12, 2021 2:40 pm
Forum: Pictures and Videos
Topic: Conjugation of oxytrichidae
Replies: 2
Views: 1185

Conjugation of oxytrichidae

Conjugation of Oxytrichidae (I think it's Oxytrichidae?) with 40x, 100x, 400x, and 600x magnification. Water sample was from a small stream near me. It was the first time I saw ciliate conjugation in one of my own samples, so that was pretty cool!!

by mazo4033
Sun Apr 25, 2021 1:44 am
Forum: Identification help
Topic: Amoeba? (Marine sample)
Replies: 10
Views: 5168

Re: Amoeba? (Marine sample)

Though I still think that this might be an amoeba preparing for division, given that it looks very similar to that, I do have an interesting image. I had been reading the book Handbook of Protozoology by Richard Kudo when I encountered an organism of a shape similar to one I had only seen once befor...
by mazo4033
Sat Apr 24, 2021 4:08 pm
Forum: Pictures and Videos
Topic: Actinocyclus subtilis Video
Replies: 8
Views: 2327

Re: Actinocyclus subtilis Video

Very cool video, 75RR! I love that you were able to catch the spinning movement and girdle view. That's not something seen too often :D
by mazo4033
Wed Apr 07, 2021 6:50 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 12592

Re: Slide Making with 61°C Wax

Great and very informative post, John, thanks for sharing! It is always a treat to read these kinds of posts :D Wonderful stain result as well with the safranin + alacian blue, especially on the monbretia flower. Your posts and advice do have a big impact - per your advice on a different post, I wen...
by mazo4033
Sat Apr 03, 2021 5:45 pm
Forum: Identification help
Topic: Staurastrum?
Replies: 2
Views: 1439

Re: Staurastrum?

Great photo! Yes, I think you got the identification correct, it is Staurastrum which is a desmid. Maybe Staurastrum planctonicum but from an apical view, not a frontal view.
by mazo4033
Fri Mar 26, 2021 2:56 pm
Forum: Pictures and Videos
Topic: Seed Shrimp
Replies: 4
Views: 1855

Re: Seed Shrimp

Very nice picture!! I like the polarized lighting that gives a rainbow-like effect.
by mazo4033
Thu Mar 25, 2021 8:49 pm
Forum: Identification help
Topic: Coleps?
Replies: 6
Views: 2550

Re: Coleps?

It is in the family Colepidae, but is not necessarily Coleps . The old genus Coleps has been split, and identification now requires a close view of the calcified armour & spines, to rule out Nolandia , Reticoleps , Pinacocoleps , etc. We don't see enough detail, here, for ID to genus. Fascinating, ...
by mazo4033
Thu Mar 25, 2021 8:44 pm
Forum: Pictures and Videos
Topic: Euglenoid
Replies: 2
Views: 1212

Re: Euglenoid

Thank you, Bruce! :D