Search found 19 matches

by Micro_UTRG
Sun Oct 30, 2022 8:38 pm
Forum: For forum members who want to buy and sell equipment
Topic: For Sale: Olympus CH-2
Replies: 0
Views: 589

For Sale: Olympus CH-2

My old Olympus CH-2/ CH-S served me well to learn about microscopy, but since I purchased a BX it is collecting dust. It comes with: Cable, obviously Binocular head (clear and moves smoothly) WHK 10x 18L eyepieces (Some coating loss is evident) Right hand stage (stiff, but easily fixed) 4 Achromatic...
by Micro_UTRG
Sun Oct 30, 2022 7:14 pm
Forum: For forum members who want to buy and sell equipment
Topic: Olympus BX items for sale
Replies: 11
Views: 2221

Re: Olympus BX items for sale

Hi Pete.

I would like to ask if you have a U-TRU, U-TRUS or a trinocular head for a BX50.

I would also be interested in prices for the Universal condenser, U-D6RE, U-DICT (or equivalent) and U-ANT.

Thanks.
by Micro_UTRG
Thu Jan 06, 2022 8:45 pm
Forum: Illumination Techniques
Topic: Problems with my Oblique Lighting
Replies: 16
Views: 5309

Re: Problems with my Oblique Lighting

The posted diatom images seem to show non-optimized brightness of field. Thanks for your reply Hobbyst46. That's caused by the way I'm taking the photographs. I used to do an afocal projection onto my DSLR with a 50mm lens, but I kept seeing tons of dust spots, so now I do a "direct" projection. I ...
by Micro_UTRG
Wed Jan 05, 2022 5:45 am
Forum: Illumination Techniques
Topic: Problems with my Oblique Lighting
Replies: 16
Views: 5309

Re: Problems with my Oblique Lighting

3/3 These next images were made with my 40x objective. N71_4572.jpg N71_4573.jpg N71_4574.jpg N71_4575.jpg N71_4576.jpg This configuration offers much less flexibility. The relief effect can be noticed, but it is not nearly as pronounced, the cell also never starts glowing and I certainly don't reac...
by Micro_UTRG
Wed Jan 05, 2022 5:34 am
Forum: Illumination Techniques
Topic: Problems with my Oblique Lighting
Replies: 16
Views: 5309

Re: Problems with my Oblique Lighting

2/3 Here is the second part of images with the 10x objective. N71_4568.jpg N71_4569.jpg N71_4570.jpg N71_4571.jpg The 3D effect is even greater; the cell walls start glowing and the chromatic aberration is more noticeable. At some point it can be removed with Lightroom, but at the end it is just gre...
by Micro_UTRG
Wed Jan 05, 2022 5:30 am
Forum: Illumination Techniques
Topic: Problems with my Oblique Lighting
Replies: 16
Views: 5309

Problems with my Oblique Lighting

Hi everyone. I have been enjoying my Olympus BX50. The U-PCD is a very good condenser and with one wheel I can change between Brightfield and Darkfield, but I like my Oblique better and it is my most used technique. However, it works best with my 10x objective only, the 4x can be used, but only a sm...
by Micro_UTRG
Mon Feb 01, 2021 9:45 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB Olympus BX40
Replies: 5
Views: 2450

Re: WTB Olympus BX40

Here's one for $2000 and seller takes offers. https://www.ebay.com/itm/Olympus-BX40-Microscope-with-3-Objectives-with-Sharp-Clear-Optics-/274425469516?_trksid=p2349624.m46890.l49292 Thanks, but it doesn't ship to Mexico unfortunately, even though it is in the USA. BX microscopes seem to be fairly a...
by Micro_UTRG
Mon Feb 01, 2021 2:05 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB Olympus BX40
Replies: 5
Views: 2450

WTB Olympus BX40

Hi Everyone.

I currently have an Olympus CH-2, but I want to upgrade to an infinity corrected system.
I would like to buy an Olympus BX40 or Bx41 with a 10x and 40x only to keep costs below 2,200 USD if that´s possible.

Thanks
Uriel
by Micro_UTRG
Mon Jul 27, 2020 1:17 am
Forum: Specimens, samples and slides
Topic: Staining a Chironomid Larva
Replies: 2
Views: 2058

Re: Staining a Chironomid Larva

That would be cool, but unfortunately I don't have access to a Microtome,Confocal or Fluorescence microscope. Can Lactophenol be used to mount such specimens?
by Micro_UTRG
Sun Jul 26, 2020 7:55 pm
Forum: Specimens, samples and slides
Topic: Staining a Chironomid Larva
Replies: 2
Views: 2058

Staining a Chironomid Larva

I've caught a Chironomid larva that I want to mount. As of right now, I've it stored in 70% Et OH. Is there a protocol for staining the nervous system and/or muscle tissue? I've a Polarization setup to see the muscle fibers, but I would like to view more things. Will Luxol Fast Blue work? How about ...
by Micro_UTRG
Sun Jul 26, 2020 7:50 pm
Forum: Specimens, samples and slides
Topic: Picric acid substitute in Johansen's Safranin-Fast Green Protocol
Replies: 5
Views: 3177

Re: Picric acid substitute in Johansen's Safranin-Fast Green Protocol

I've only done Sass Safranin - Fast Green on Plant tissue sections. However, I think a mild acid like Acetic acid is enough to differentiate the Safranin. Not that I'm an expert, but I think that Differentiation just refers to the Safranin being de-stained more slowly from lignified tissues or nucle...
by Micro_UTRG
Thu Jul 09, 2020 2:52 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB: Olympus short-barrel PlanApo 40x and 100x
Replies: 5
Views: 2962

Re: WTB: Olympus short-barrel PlanApo 40x and 100x

Hi.
Although not exactly what you requested, I have a Short barrel FL 40x 0.75. I got it for my CH-2 but didn't know it wouldn't function properly.
Please contact me if you want it.
Pd. I'm in Mexico
by Micro_UTRG
Thu Jul 09, 2020 1:52 am
Forum: Microscopes and optics
Topic: Some problems with my microscope
Replies: 11
Views: 3992

Re: Some problems with my microscope

Thanks everyone, That is something I didn't thought of.
I'm going to get an Olympus condenser, since most of you agree it is best.
I will also sell my 40x Objective and try to get a new Long Barrel one. Are other brand Objectives totally compatible?
by Micro_UTRG
Wed Jul 08, 2020 4:26 pm
Forum: Microscopes and optics
Topic: Some problems with my microscope
Replies: 11
Views: 3992

Re: Some problems with my microscope

Well, the condenser is cheap, high NA and of metal construction, I'm a fan of rare looking equipment. It comes with its own stand, but if there is a problem I would rather use an Olympus. :D Regarding the BH2 vs Infinity scopes, the newer objectives have improved coatings, aspherical elements and so...
by Micro_UTRG
Wed Jul 08, 2020 3:18 pm
Forum: Microscopes and optics
Topic: Some problems with my microscope
Replies: 11
Views: 3992

Re: Some problems with my microscope

As suggested earlier, just a complete 1.25 na Olympus condenser that fits your scope would work well. Olympus also made a better color corrected 1.4na condenser, but the extra $100 it might cost won't be all that obvious until you, say, start taking photos with a 100x oil immersion lens, with the c...
by Micro_UTRG
Wed Jul 08, 2020 4:52 am
Forum: Microscopes and optics
Topic: CH-2 objective options
Replies: 3
Views: 2646

Re: CH-2 objective options

I just ordered a FL 40x 0.75 from Ebay for less than 75 USD for my CH-2 and the stage doesn't go far enough.
by Micro_UTRG
Wed Jul 08, 2020 4:16 am
Forum: Microscopes and optics
Topic: Some problems with my microscope
Replies: 11
Views: 3992

Re: Some problems with my microscope

Thanks for the response.
I only had a 4x, 10x, 20x and a 100x, so a 40x was a must. Could you tell me a bit more about the extender? Advantages and Disadvantages.
Also, what are some good and rare Condensers? Say, below 150 USD.
by Micro_UTRG
Wed Jul 08, 2020 1:32 am
Forum: Microscopes and optics
Topic: Some problems with my microscope
Replies: 11
Views: 3992

Some problems with my microscope

Hi everyone, I have several questions regarding my microscope: I bought an Olympus CH-2 with a set of D Achromats and I recently bought a Short barrel FL 40x 0.75. The Shrot barrel objective requires much closer focusing distance than my microscope can provide. How can I work around the short barrel...